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The map label for this gene is trmD [H]

Identifier: 225630685

GI number: 225630685

Start: 1051762

End: 1052472

Strand: Reverse

Name: trmD [H]

Synonym: WRi_009780

Alternate gene names: 225630685

Gene position: 1052472-1051762 (Counterclockwise)

Preceding gene: 225630687

Following gene: 225630684

Centisome position: 72.79

GC content: 35.58

Gene sequence:

>711_bases
ATGGCATTCAATGTTACAATATTAACCATATTCCCAGAAATGTTCCCGGGGTTTTTGAACTATTCTCTTGCCGGAAAAGC
GTTAGAAAAAAAAATATGGAACCTTGAAGTAATAAATATTCGTTCTTTTGCGAAAGATAAACATTCAACTGTGGATGATG
TTCCATATGGAGGTGGAGCAGGAATGGTTATGCGTTCTGATGTAGTTGGTGATGCAGTAGATAGTATGCTCTCTGTTCAT
AAAAACACTAAGTTTATTTACATGACTCCATCTGGAACTAAGTTTAATCAGAATATTGCCAGAGAATTATTAGAGTTTCC
TCATATAACAATATTGTGTGGTCGATTTGAAGGTATTGACCAAAGGATAATTGATGCGTATACTCCTTATGAGTTAAGTA
TTGGAGATTATATACTTTCAGGAGGTGAGCCAGCTGCAATGGTGGTTCTTGATGCATGCATTAGACTTCTTCCAGGTGTA
GTAAATAATACCGATAGTATTACTGAAGAAAGTTTTAATTATGGTGGTGGTGTACTTGAATATCCTCAATATACTAGACC
TGAGCAGTGGAAGGGATATAAAGTACCTGAGGTTTTGTTATCTGGCAATCACAAAAAAATAAGTGATTGGAGGCAGAAGC
AGTCTCATGTTATAACAAAAAAGCGTAGGCCTGAATTATTGAGTGGAGAGATAAATGACAAATTTACTTAA

Upstream 100 bases:

>100_bases
TCGTATATCGTTTTATTCAGTGACATGTTTAATTTAACTATATATTATATCAATTTACTATTCTTAGAGTATAGCCCCAA
ATAACTTAAAATAAACCTTA

Downstream 100 bases:

>100_bases
AAAATTTAACGAACAGCAAATGCAAGTGTTAGCTAAAGAAATACCAGAATTTCGTCCTGGCGATGATTTGAAGGTCACTT
TTAAAGTAGTTGACGGTACA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MAFNVTILTIFPEMFPGFLNYSLAGKALEKKIWNLEVINIRSFAKDKHSTVDDVPYGGGAGMVMRSDVVGDAVDSMLSVH
KNTKFIYMTPSGTKFNQNIARELLEFPHITILCGRFEGIDQRIIDAYTPYELSIGDYILSGGEPAAMVVLDACIRLLPGV
VNNTDSITEESFNYGGGVLEYPQYTRPEQWKGYKVPEVLLSGNHKKISDWRQKQSHVITKKRRPELLSGEINDKFT

Sequences:

>Translated_236_residues
MAFNVTILTIFPEMFPGFLNYSLAGKALEKKIWNLEVINIRSFAKDKHSTVDDVPYGGGAGMVMRSDVVGDAVDSMLSVH
KNTKFIYMTPSGTKFNQNIARELLEFPHITILCGRFEGIDQRIIDAYTPYELSIGDYILSGGEPAAMVVLDACIRLLPGV
VNNTDSITEESFNYGGGVLEYPQYTRPEQWKGYKVPEVLLSGNHKKISDWRQKQSHVITKKRRPELLSGEINDKFT
>Mature_235_residues
AFNVTILTIFPEMFPGFLNYSLAGKALEKKIWNLEVINIRSFAKDKHSTVDDVPYGGGAGMVMRSDVVGDAVDSMLSVHK
NTKFIYMTPSGTKFNQNIARELLEFPHITILCGRFEGIDQRIIDAYTPYELSIGDYILSGGEPAAMVVLDACIRLLPGVV
NNTDSITEESFNYGGGVLEYPQYTRPEQWKGYKVPEVLLSGNHKKISDWRQKQSHVITKKRRPELLSGEINDKFT

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=224, Percent_Identity=43.75, Blast_Score=187, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 26516; Mature: 26385

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFNVTILTIFPEMFPGFLNYSLAGKALEKKIWNLEVINIRSFAKDKHSTVDDVPYGGGA
CCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHCCCCCCCCCCCCCCCC
GMVMRSDVVGDAVDSMLSVHKNTKFIYMTPSGTKFNQNIARELLEFPHITILCGRFEGID
CCEEECHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCH
QRIIDAYTPYELSIGDYILSGGEPAAMVVLDACIRLLPGVVNNTDSITEESFNYGGGVLE
HHHHHCCCCEEEECCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCEEE
YPQYTRPEQWKGYKVPEVLLSGNHKKISDWRQKQSHVITKKRRPELLSGEINDKFT
CCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCC
>Mature Secondary Structure 
AFNVTILTIFPEMFPGFLNYSLAGKALEKKIWNLEVINIRSFAKDKHSTVDDVPYGGGA
CEEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHCCCCCCCCCCCCCCCC
GMVMRSDVVGDAVDSMLSVHKNTKFIYMTPSGTKFNQNIARELLEFPHITILCGRFEGID
CCEEECHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCH
QRIIDAYTPYELSIGDYILSGGEPAAMVVLDACIRLLPGVVNNTDSITEESFNYGGGVLE
HHHHHCCCCEEEECCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCEEE
YPQYTRPEQWKGYKVPEVLLSGNHKKISDWRQKQSHVITKKRRPELLSGEINDKFT
CCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA