Click here to switch to the map view.

The map label for this gene is birA [H]

Identifier: 225630637

GI number: 225630637

Start: 990745

End: 991617

Strand: Direct

Name: birA [H]

Synonym: WRi_009160

Alternate gene names: 225630637

Gene position: 990745-991617 (Clockwise)

Preceding gene: 225630636

Following gene: 225630638

Centisome position: 68.52

GC content: 35.17

Gene sequence:

>873_bases
GTGATCCCTGAGGGTTTTCATATTCATCATTACAAAGAAGTTTCAAGCACTAATAAAGAAGCGTTGGATTTAATTGATAA
AGGAATATCAAATGAAACCGCTATTATTGCCGACAAACAAACAGAGGGTAGAGGGCGTACCGGAAAAAGTTGGATTTCTC
CCGAGGGTAATTTTTATGCAAGTTTGGTAATAAACCTCTTTAACGATTATCTGAATGAAAGCCAGCTTTCGGTGTCATTC
CAGCGCGTGACGCTGGAATCTAGAAAAAAAGAAGAATGGATCCCAGTGTCAAGCACTGGGATGACATCAGAGGGCATTGG
GATGACAGAGAAGGGTTTGGTGAATAAAATTGCGGATGTTAGCAAATTAACAGAATTGACTTTTGTTACTGCTCTTACTG
TTGGAAATACTATACTGTCATTTATAAATGGTCTAAATCTCCAGTATAAGTGGCCAAATGATGTCCTTATTGATGGCAAG
AAAATAAGTGGAATATTGCTTGAAAAAAGATCCAATTCGAATTGGCTTATTGTAGGAATTGGAATTAATGTCAGTCATGC
ACCACTTCCAGGAACAACGTGCATTAGCAATTACGGTGAGTCTGTGTCTAACATAGATCTGTTAAAGGAATTAATAATAA
ATTTTAATAAGCTAAGAAAGCAATGGCTATTTGATGGGTTTTATGCTATAAGAGAAATGTGGTTAAAAAAAGCATTCAAA
ATGAATGAGCAAATCAGTGTAAAGTTAGCTGACAAATTGTATGAAGGAATTTTTGCTGATATAGATAAAAGTGGTAAATT
GGTGTTGCAGCAAAAGGATGAAAGCTTAATTTATTTTGATGCAGGTGAGTTATTTATTAACGATATATTATGA

Upstream 100 bases:

>100_bases
ATTCTAAGGGTCTATTCATTATTACTTCAGTGGCTTCACTGATCAACCTCGTTCTTTTCTTGTACGTTTTACTCACTCAT
TGAACTAAAGGACTGACAAG

Downstream 100 bases:

>100_bases
ACACGTATGTGATTTTTGCTTACCTTATTAGTCTTACATTGATTACCGGAGAACTGATTCTTACCATTTCTTGCTATATT
AAAAGTAAAAAAATTCTAGA

Product: biotin-[acetyl-coa-carboxylase] ligase, putative

Products: NA

Alternate protein names: Biotin--protein ligase [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MIPEGFHIHHYKEVSSTNKEALDLIDKGISNETAIIADKQTEGRGRTGKSWISPEGNFYASLVINLFNDYLNESQLSVSF
QRVTLESRKKEEWIPVSSTGMTSEGIGMTEKGLVNKIADVSKLTELTFVTALTVGNTILSFINGLNLQYKWPNDVLIDGK
KISGILLEKRSNSNWLIVGIGINVSHAPLPGTTCISNYGESVSNIDLLKELIINFNKLRKQWLFDGFYAIREMWLKKAFK
MNEQISVKLADKLYEGIFADIDKSGKLVLQQKDESLIYFDAGELFINDIL

Sequences:

>Translated_290_residues
MIPEGFHIHHYKEVSSTNKEALDLIDKGISNETAIIADKQTEGRGRTGKSWISPEGNFYASLVINLFNDYLNESQLSVSF
QRVTLESRKKEEWIPVSSTGMTSEGIGMTEKGLVNKIADVSKLTELTFVTALTVGNTILSFINGLNLQYKWPNDVLIDGK
KISGILLEKRSNSNWLIVGIGINVSHAPLPGTTCISNYGESVSNIDLLKELIINFNKLRKQWLFDGFYAIREMWLKKAFK
MNEQISVKLADKLYEGIFADIDKSGKLVLQQKDESLIYFDAGELFINDIL
>Mature_290_residues
MIPEGFHIHHYKEVSSTNKEALDLIDKGISNETAIIADKQTEGRGRTGKSWISPEGNFYASLVINLFNDYLNESQLSVSF
QRVTLESRKKEEWIPVSSTGMTSEGIGMTEKGLVNKIADVSKLTELTFVTALTVGNTILSFINGLNLQYKWPNDVLIDGK
KISGILLEKRSNSNWLIVGIGINVSHAPLPGTTCISNYGESVSNIDLLKELIINFNKLRKQWLFDGFYAIREMWLKKAFK
MNEQISVKLADKLYEGIFADIDKSGKLVLQQKDESLIYFDAGELFINDIL

Specific function: Activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins [H]

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR003142
- InterPro:   IPR004143 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 32592; Mature: 32592

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPEGFHIHHYKEVSSTNKEALDLIDKGISNETAIIADKQTEGRGRTGKSWISPEGNFYA
CCCCCCEEEEEHHHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHH
SLVINLFNDYLNESQLSVSFQRVTLESRKKEEWIPVSSTGMTSEGIGMTEKGLVNKIADV
HHHHHHHHHHHCCHHHEEEHEEEEHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
SKLTELTFVTALTVGNTILSFINGLNLQYKWPNDVLIDGKKISGILLEKRSNSNWLIVGI
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCEECEEEEEECCCCCEEEEEE
GINVSHAPLPGTTCISNYGESVSNIDLLKELIINFNKLRKQWLFDGFYAIREMWLKKAFK
ECCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
MNEQISVKLADKLYEGIFADIDKSGKLVLQQKDESLIYFDAGELFINDIL
CCCCEEHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCHHHHHHCC
>Mature Secondary Structure
MIPEGFHIHHYKEVSSTNKEALDLIDKGISNETAIIADKQTEGRGRTGKSWISPEGNFYA
CCCCCCEEEEEHHHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHH
SLVINLFNDYLNESQLSVSFQRVTLESRKKEEWIPVSSTGMTSEGIGMTEKGLVNKIADV
HHHHHHHHHHHCCHHHEEEHEEEEHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
SKLTELTFVTALTVGNTILSFINGLNLQYKWPNDVLIDGKKISGILLEKRSNSNWLIVGI
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCEECEEEEEECCCCCEEEEEE
GINVSHAPLPGTTCISNYGESVSNIDLLKELIINFNKLRKQWLFDGFYAIREMWLKKAFK
ECCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
MNEQISVKLADKLYEGIFADIDKSGKLVLQQKDESLIYFDAGELFINDIL
CCCCEEHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]