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The map label for this gene is lysC [H]

Identifier: 225630627

GI number: 225630627

Start: 975878

End: 977110

Strand: Direct

Name: lysC [H]

Synonym: WRi_008990

Alternate gene names: 225630627

Gene position: 975878-977110 (Clockwise)

Preceding gene: 225630626

Following gene: 225630628

Centisome position: 67.49

GC content: 34.96

Gene sequence:

>1233_bases
ATGGCTTGCAAGCTGCTATCTCTACAAAAACTATATTTTTCTTCAGAAGAGAGTTATATTGTAACTATGGACAAAATAAT
CATAAAAAAATTTGGTGGGACTTCGCTAACTGATTTAAACCGAGTTGCAAATTTGATAAAAAACGATATTGAGAAAGGTT
GTAATGTAATCGTTGTTGTATCTGCTGTTGCAGGATTTACTGACCAAATGGCTTTTCAGGCTAGGCAAATCTCAAATTTA
AGTTGCAGACAAGAGTTATCAGAGTATGACGTTATGCTTTCAGCAGGAGAACAAATTTCTTGCGGTCTATTAGCTATCAC
TCTCCAATCGATCGGAGTTAATGCTAAATCGTGGCTTGCTTGGCAGTTACCAATTGTAACTGATGATTTTTATTCTGAGT
CTAAAATAAAAACAATAAAGATTGACCGTGTGAAAAGATCTTTTGCTGAGGGTTATACTGCTGCGATCATCGCTGGTTTT
CAGGGTATAAATGATGATAGAATCACTACTTTTGGAAGAGGAGGCTCTGATATATCAGCAGTTGCCTTCACGGTAGCCTT
TGGTGTTAGAACTTGCGAAATTTTTACTGATATTGATGGAATATATACAGCAGACCCGAGAATTGTTCCAAAGGCACGCA
AGCTCAAATTTATCTCTTACGATGAAATGTTGGAAATGTCGTCATCTGGTGCTAAAATACTGCATAATCGTTCAGTACAA
CTTGCAATGAAACATAACATTAAAGTGCAAGTGCTATCCACTTTTAAGGAAGTAGAAGGCACCACAGTGCTACACAAAAG
GGATGTACTAGAGAGATACTTAATTACTGGAATAACTTATAGCACTAATGAAGCTCTTGTAACTTTCACTAACCTTGCAA
ATAACTTGCGTACTTTAAGAGATATAGTAGGGGCAAACGTTAAAATTGATATGGTACATGGGTCAAGTTTTGTTATCTCC
AAATTTGATATTGATTTAATGGAAAAGTTACTGAATAAGAATGAAAATTATGCTATAAACGATAATGTAGCTAAAATTTC
AATAATTGGTATTGGTGTTATGTCTAACACTGAAGTGATGCACCGTACACTCAAGGTTTTAAGCGAAAAAAAGATAGAAA
TACTTGCTATCACAACATCCGAAATCAAAATCAGTATAATTGTTCAAAAAGAATACGTTGTAGCTTTGGTCAAAGATTTA
CATACTGAGTATGAGCTTGATATGCAACACTAG

Upstream 100 bases:

>100_bases
CATTTGCTGTGCAATTTACCTTCAAAAATGAATGTTCGTACAGCTATGGAATGAATCGTGGTATGACGCGTTAGCTATAG
CTTTGTCTTCCCTTTTCTGA

Downstream 100 bases:

>100_bases
GATAGGCTCGACGTCCAGTTGTCTAAGAGTATGTCTTGAAAAGGAATGAGGAAAAAAATATGATAGTGTAAGTAAAAAAT
AGGAGGGAAAATTGTATCCA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MACKLLSLQKLYFSSEESYIVTMDKIIIKKFGGTSLTDLNRVANLIKNDIEKGCNVIVVVSAVAGFTDQMAFQARQISNL
SCRQELSEYDVMLSAGEQISCGLLAITLQSIGVNAKSWLAWQLPIVTDDFYSESKIKTIKIDRVKRSFAEGYTAAIIAGF
QGINDDRITTFGRGGSDISAVAFTVAFGVRTCEIFTDIDGIYTADPRIVPKARKLKFISYDEMLEMSSSGAKILHNRSVQ
LAMKHNIKVQVLSTFKEVEGTTVLHKRDVLERYLITGITYSTNEALVTFTNLANNLRTLRDIVGANVKIDMVHGSSFVIS
KFDIDLMEKLLNKNENYAINDNVAKISIIGIGVMSNTEVMHRTLKVLSEKKIEILAITTSEIKISIIVQKEYVVALVKDL
HTEYELDMQH

Sequences:

>Translated_410_residues
MACKLLSLQKLYFSSEESYIVTMDKIIIKKFGGTSLTDLNRVANLIKNDIEKGCNVIVVVSAVAGFTDQMAFQARQISNL
SCRQELSEYDVMLSAGEQISCGLLAITLQSIGVNAKSWLAWQLPIVTDDFYSESKIKTIKIDRVKRSFAEGYTAAIIAGF
QGINDDRITTFGRGGSDISAVAFTVAFGVRTCEIFTDIDGIYTADPRIVPKARKLKFISYDEMLEMSSSGAKILHNRSVQ
LAMKHNIKVQVLSTFKEVEGTTVLHKRDVLERYLITGITYSTNEALVTFTNLANNLRTLRDIVGANVKIDMVHGSSFVIS
KFDIDLMEKLLNKNENYAINDNVAKISIIGIGVMSNTEVMHRTLKVLSEKKIEILAITTSEIKISIIVQKEYVVALVKDL
HTEYELDMQH
>Mature_409_residues
ACKLLSLQKLYFSSEESYIVTMDKIIIKKFGGTSLTDLNRVANLIKNDIEKGCNVIVVVSAVAGFTDQMAFQARQISNLS
CRQELSEYDVMLSAGEQISCGLLAITLQSIGVNAKSWLAWQLPIVTDDFYSESKIKTIKIDRVKRSFAEGYTAAIIAGFQ
GINDDRITTFGRGGSDISAVAFTVAFGVRTCEIFTDIDGIYTADPRIVPKARKLKFISYDEMLEMSSSGAKILHNRSVQL
AMKHNIKVQVLSTFKEVEGTTVLHKRDVLERYLITGITYSTNEALVTFTNLANNLRTLRDIVGANVKIDMVHGSSFVISK
FDIDLMEKLLNKNENYAINDNVAKISIIGIGVMSNTEVMHRTLKVLSEKKIEILAITTSEIKISIIVQKEYVVALVKDLH
TEYELDMQH

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=348, Percent_Identity=27.0114942528736, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1790455, Length=357, Percent_Identity=26.890756302521, Blast_Score=89, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6320893, Length=192, Percent_Identity=24.4791666666667, Blast_Score=74, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 45698; Mature: 45566

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACKLLSLQKLYFSSEESYIVTMDKIIIKKFGGTSLTDLNRVANLIKNDIEKGCNVIVVV
CCCCHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
SAVAGFTDQMAFQARQISNLSCRQELSEYDVMLSAGEQISCGLLAITLQSIGVNAKSWLA
EHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCCCEEEEHHHCCCCCCCEEE
WQLPIVTDDFYSESKIKTIKIDRVKRSFAEGYTAAIIAGFQGINDDRITTFGRGGSDISA
EECCEEECCCCCCCCEEEEEHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCCHHH
VAFTVAFGVRTCEIFTDIDGIYTADPRIVPKARKLKFISYDEMLEMSSSGAKILHNRSVQ
HHHHHHHCCEEEEEEECCCCEEECCCCCCCCHHHEEEECHHHHHHHCCCCCEEEECCCEE
LAMKHNIKVQVLSTFKEVEGTTVLHKRDVLERYLITGITYSTNEALVTFTNLANNLRTLR
EEEECCCEEEEEHHHHHCCCCEEEHHHHHHHHHHHHCEEEECCCEEEEEHHHHHHHHHHH
DIVGANVKIDMVHGSSFVISKFDIDLMEKLLNKNENYAINDNVAKISIIGIGVMSNTEVM
HHHCCCEEEEEEECCEEEEEECCHHHHHHHHCCCCCEEECCCCEEEEEEEEEEECCHHHH
HRTLKVLSEKKIEILAITTSEIKISIIVQKEYVVALVKDLHTEYELDMQH
HHHHHHHCCCCEEEEEEEECCEEEEEEEEHHHHHHHHHHHCCCCEEECCC
>Mature Secondary Structure 
ACKLLSLQKLYFSSEESYIVTMDKIIIKKFGGTSLTDLNRVANLIKNDIEKGCNVIVVV
CCCHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
SAVAGFTDQMAFQARQISNLSCRQELSEYDVMLSAGEQISCGLLAITLQSIGVNAKSWLA
EHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCCCEEEEHHHCCCCCCCEEE
WQLPIVTDDFYSESKIKTIKIDRVKRSFAEGYTAAIIAGFQGINDDRITTFGRGGSDISA
EECCEEECCCCCCCCEEEEEHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCCHHH
VAFTVAFGVRTCEIFTDIDGIYTADPRIVPKARKLKFISYDEMLEMSSSGAKILHNRSVQ
HHHHHHHCCEEEEEEECCCCEEECCCCCCCCHHHEEEECHHHHHHHCCCCCEEEECCCEE
LAMKHNIKVQVLSTFKEVEGTTVLHKRDVLERYLITGITYSTNEALVTFTNLANNLRTLR
EEEECCCEEEEEHHHHHCCCCEEEHHHHHHHHHHHHCEEEECCCEEEEEHHHHHHHHHHH
DIVGANVKIDMVHGSSFVISKFDIDLMEKLLNKNENYAINDNVAKISIIGIGVMSNTEVM
HHHCCCEEEEEEECCEEEEEECCHHHHHHHHCCCCCEEECCCCEEEEEEEEEEECCHHHH
HRTLKVLSEKKIEILAITTSEIKISIIVQKEYVVALVKDLHTEYELDMQH
HHHHHHHCCCCEEEEEEEECCEEEEEEEEHHHHHHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]