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The map label for this gene is folKP [H]

Identifier: 225630594

GI number: 225630594

Start: 932453

End: 933736

Strand: Reverse

Name: folKP [H]

Synonym: WRi_008620

Alternate gene names: 225630594

Gene position: 933736-932453 (Counterclockwise)

Preceding gene: 225630595

Following gene: 225630593

Centisome position: 64.58

GC content: 38.24

Gene sequence:

>1284_bases
ATGATTTACATTTCTATTGGCTCAAATATGGGGAATCGCTTTTCCCATTTACAAAAGGCTGCTCAGTTACTGCAGGAACG
CTATTTTAAAAATTTAAAATCTTCAATTATTCTGGAAACTAAGGCTATTTTACCAAATGATGCTCCACCTGGGTGGGACA
GGCCATTTCTAAATATGGTTGTGTATGGAAGTTGTTCTTCTTCTCCTGAAGAGCTGTTAAAGGGCCTTAAACAAATTGAA
TGTGACATTGGCCGTCTGCAGGTCTATGAAAAATGGGCACCTCGCGTTATTGATTTGGATATTTTGTTATGGGATGACCT
GACACTTGATACACCCTACCTTAAGATTCCTCACCCAGAATTGATAAACAGACCATTTTTGCTTCACTTGATGGCAATGC
TGAGTCCCATGGCAGTAGTTAACAAAACGTTTGGTACTATTAACCCTAACATTAAGGATTGCTTTTTAAGGAGTTTTACG
CTTTCACCAGAGCTTGTAGGTATTGTTAATATTACTCCTGATTCATTTTCAGATGGTGGTCTTTATTATGATGCAGATCA
AGCAACCAAGCAGGCACTGCGGCTGTTATCAGATGGTGCAAGCATAGTTGAACTTGGTGCTCAATCAACAAGACCTGGCT
CCTCAATACAAACTCCAAAAGCAGAATATGCACGCTTAAAACCAGTGCTTGATAATCTCAATCAGTATATGAAAGTTGGT
GACATTAAAGTCAGCATTGATAGTTTCTGGCCAGATGTTATTTTGAATGTTTTGGAGCACTACAACATTGCCTGGGTAAA
CGATCAGAAGGGAGATCTAGATGATAATACCTTAAAAGCGATTGCTAGCAGTGGATGTAGTATCGTTATTATGCATTCAC
TCTCCATACCACCACATAGTGACAACATTATCCCAAGTGACATTGATCCAATTGATACCATAAATAATTGGGCAGAAAGG
AACATTAATAGATTACTAGCTCTAGGTTTTGATCAAAGCTCGATCATTATTGATCCTGGTATTGGTTTTGGCAAATCTAT
GTACCAGAATATCCAGATTTTACGCAGTATAGAAACGTTACAAAATTTTGGCTGCAAAGTTCTGGTTGGTCATTCTAGAA
AGTCATTTATTTCTTCTTTTTCTACAGAACCTGTCTTTAACCGAGATTTAGAAACAATTGCTTTATCATCTGCATTGCAC
AACAAGGTTGATTTCCTTCGAGTGCATAATGTGCGTGATCACATGCGATTTTTTGTAGCTCAAGCTGCTTTACAGGGATG
CTAG

Upstream 100 bases:

>100_bases
TTATTATTTCTTCTATAGGCGTAACTATTCACAAAGTTGCACCACCTGTCCCCAGTGTGCATGGAGGTGTTTTCTTTACT
TATCGTAATGCGTTGCAAGA

Downstream 100 bases:

>100_bases
TTATCGGAATTATGGCTGTTGATCCCAAGGGGGTAATTGGAATAAATAATGGATTGCCCTGGCATTATCCAAGTGAGTTA
GATCATTTTCGTCAGGTGAC

Product: Folate synthesis bifunctional protein

Products: NA

Alternate protein names: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase; 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK; Dihydropteroate synthase; DHPS; Dihydropteroate pyrophosphorylase [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MIYISIGSNMGNRFSHLQKAAQLLQERYFKNLKSSIILETKAILPNDAPPGWDRPFLNMVVYGSCSSSPEELLKGLKQIE
CDIGRLQVYEKWAPRVIDLDILLWDDLTLDTPYLKIPHPELINRPFLLHLMAMLSPMAVVNKTFGTINPNIKDCFLRSFT
LSPELVGIVNITPDSFSDGGLYYDADQATKQALRLLSDGASIVELGAQSTRPGSSIQTPKAEYARLKPVLDNLNQYMKVG
DIKVSIDSFWPDVILNVLEHYNIAWVNDQKGDLDDNTLKAIASSGCSIVIMHSLSIPPHSDNIIPSDIDPIDTINNWAER
NINRLLALGFDQSSIIIDPGIGFGKSMYQNIQILRSIETLQNFGCKVLVGHSRKSFISSFSTEPVFNRDLETIALSSALH
NKVDFLRVHNVRDHMRFFVAQAALQGC

Sequences:

>Translated_427_residues
MIYISIGSNMGNRFSHLQKAAQLLQERYFKNLKSSIILETKAILPNDAPPGWDRPFLNMVVYGSCSSSPEELLKGLKQIE
CDIGRLQVYEKWAPRVIDLDILLWDDLTLDTPYLKIPHPELINRPFLLHLMAMLSPMAVVNKTFGTINPNIKDCFLRSFT
LSPELVGIVNITPDSFSDGGLYYDADQATKQALRLLSDGASIVELGAQSTRPGSSIQTPKAEYARLKPVLDNLNQYMKVG
DIKVSIDSFWPDVILNVLEHYNIAWVNDQKGDLDDNTLKAIASSGCSIVIMHSLSIPPHSDNIIPSDIDPIDTINNWAER
NINRLLALGFDQSSIIIDPGIGFGKSMYQNIQILRSIETLQNFGCKVLVGHSRKSFISSFSTEPVFNRDLETIALSSALH
NKVDFLRVHNVRDHMRFFVAQAALQGC
>Mature_427_residues
MIYISIGSNMGNRFSHLQKAAQLLQERYFKNLKSSIILETKAILPNDAPPGWDRPFLNMVVYGSCSSSPEELLKGLKQIE
CDIGRLQVYEKWAPRVIDLDILLWDDLTLDTPYLKIPHPELINRPFLLHLMAMLSPMAVVNKTFGTINPNIKDCFLRSFT
LSPELVGIVNITPDSFSDGGLYYDADQATKQALRLLSDGASIVELGAQSTRPGSSIQTPKAEYARLKPVLDNLNQYMKVG
DIKVSIDSFWPDVILNVLEHYNIAWVNDQKGDLDDNTLKAIASSGCSIVIMHSLSIPPHSDNIIPSDIDPIDTINNWAER
NINRLLALGFDQSSIIIDPGIGFGKSMYQNIQILRSIETLQNFGCKVLVGHSRKSFISSFSTEPVFNRDLETIALSSALH
NKVDFLRVHNVRDHMRFFVAQAALQGC

Specific function: Dhps Catalyzes The Formation Of The Immediate Precursor Of Folic Acid. It Is Implicated In Resistance To Sulfonamide. [C]

COG id: COG0294

COG function: function code H; Dihydropteroate synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082224, Length=252, Percent_Identity=30.1587301587302, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1786335, Length=138, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324073, Length=515, Percent_Identity=26.6019417475728, Blast_Score=137, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006390
- InterPro:   IPR011005
- InterPro:   IPR000550
- InterPro:   IPR000489 [H]

Pfam domain/function: PF01288 HPPK; PF00809 Pterin_bind [H]

EC number: =2.7.6.3; =2.5.1.15 [H]

Molecular weight: Translated: 47760; Mature: 47760

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS50972 PTERIN_BINDING ; PS00792 DHPS_1 ; PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYISIGSNMGNRFSHLQKAAQLLQERYFKNLKSSIILETKAILPNDAPPGWDRPFLNMV
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHCCCCCCCCCCCCCCEEEE
VYGSCSSSPEELLKGLKQIECDIGRLQVYEKWAPRVIDLDILLWDDLTLDTPYLKIPHPE
EEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEEEEEEECCCCCCCCEEECCCHH
LINRPFLLHLMAMLSPMAVVNKTFGTINPNIKDCFLRSFTLSPELVGIVNITPDSFSDGG
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCC
LYYDADQATKQALRLLSDGASIVELGAQSTRPGSSIQTPKAEYARLKPVLDNLNQYMKVG
EEECCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
DIKVSIDSFWPDVILNVLEHYNIAWVNDQKGDLDDNTLKAIASSGCSIVIMHSLSIPPHS
CEEEEHHHHCHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCC
DNIIPSDIDPIDTINNWAERNINRLLALGFDQSSIIIDPGIGFGKSMYQNIQILRSIETL
CCCCCCCCCCHHHHHHHHHHHHHHHEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHH
QNFGCKVLVGHSRKSFISSFSTEPVFNRDLETIALSSALHNKVDFLRVHNVRDHMRFFVA
HHCCCEEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAALQGC
HHHHHCC
>Mature Secondary Structure
MIYISIGSNMGNRFSHLQKAAQLLQERYFKNLKSSIILETKAILPNDAPPGWDRPFLNMV
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHCCCCCCCCCCCCCCEEEE
VYGSCSSSPEELLKGLKQIECDIGRLQVYEKWAPRVIDLDILLWDDLTLDTPYLKIPHPE
EEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEEEEEEECCCCCCCCEEECCCHH
LINRPFLLHLMAMLSPMAVVNKTFGTINPNIKDCFLRSFTLSPELVGIVNITPDSFSDGG
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCC
LYYDADQATKQALRLLSDGASIVELGAQSTRPGSSIQTPKAEYARLKPVLDNLNQYMKVG
EEECCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
DIKVSIDSFWPDVILNVLEHYNIAWVNDQKGDLDDNTLKAIASSGCSIVIMHSLSIPPHS
CEEEEHHHHCHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCC
DNIIPSDIDPIDTINNWAERNINRLLALGFDQSSIIIDPGIGFGKSMYQNIQILRSIETL
CCCCCCCCCCHHHHHHHHHHHHHHHEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHH
QNFGCKVLVGHSRKSFISSFSTEPVFNRDLETIALSSALHNKVDFLRVHNVRDHMRFFVA
HHCCCEEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAALQGC
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12682364 [H]