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The map label for this gene is trpS [H]

Identifier: 225630518

GI number: 225630518

Start: 832611

End: 833609

Strand: Direct

Name: trpS [H]

Synonym: WRi_007700

Alternate gene names: 225630518

Gene position: 832611-833609 (Clockwise)

Preceding gene: 225630517

Following gene: 225630525

Centisome position: 57.59

GC content: 34.53

Gene sequence:

>999_bases
ATGACAAGTATTGTCTTTTCAGGTATCCAGCCAAGTGGCGTACTACATTTAGGTAATTATCTTGGTGCAATCAAACAGTG
GATAGACTTACAGGATAAATACAAATCTCTTTTTTGTATTGTCGACCTACACGCAATCACAGCAAATAAGCTTCCCGCAA
GTGAATTAAAAAGTAATATTTTCAAAACAGCAGCAGCTTATATTGCATGCGGAATAGATACAGAAAAGTCCATTATTTTT
AGTCAGTCCACAGTTAGTGGTCATGCAGAACTGGGCTGGCTGCTTGGATGCTATACACCAATTGGTTGGCTTAATCGCAT
GACTCAATTTAAAGATAAAGCTGGCAGCGATAAACAAAAAGCCTCTCTTGGATTATATAGTTATCCAGTACTTATGGCTG
CAGATATATTGTTGTATCAAACTAAATACGTTCCTGTTGGTGATGATCAAAAACAGCATTTGGAGCTTGCACGTGATATT
GCATCAGCTTTTAACAATCATTATAAGCTGGATTATTTCATCGTACCTGAAATCTTAACTTTGGATTGCACATCAAGGAT
AATGAGCTTAAGGGATGGCACAAGCAAGATGAGTAAATCTGATCCTTCAGAACATTCGTGCATTAACCTTGATGATACGG
ATGACTTAATTGTCAAAAAAATAGAAAAAGCAAAAACAGATTCAATTCTAGGCTTTGCTACTTTAAAGTGCCGACCAGAA
ATAAGCAATTTGATAAACATTTATTCAGTGCTTAGTAATGTAAATGCAGAAAAAGTATGTGAAGAAGTGAACAAACATGA
CATGAAGCACTTCAAAAAAGAGTTAGCTGATTTAATTATCAGTGTTATATCACCCATACGTGAGAAAATGAATGATCTTT
TAAAAGACCAATTCCATTTACATGAAATATTAAAAAAAGGCACAAAGAAAGCAGCAGAAATTGCAACCCATAATATAAAA
GAAATCAAGGATATTATTGGATTTGCTCAATATCTGTAA

Upstream 100 bases:

>100_bases
CTGGTTATACTATCAAAAATAGGTTGCTCCGTCCTGCAATGGTTATTCTTTCTAAGAAATCTGCTGATTGTGGAAGCGAT
TAGCATGAGGTAAATGTATA

Downstream 100 bases:

>100_bases
GGTTCCAAAGTGTGATGGTGCGAACCGTCATACCGCCGCGGTATCTCAGCCGCTAACACGTAGCGGGATGACGGTTTTTC
AAATTGTCGGTAAATCTAAG

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MTSIVFSGIQPSGVLHLGNYLGAIKQWIDLQDKYKSLFCIVDLHAITANKLPASELKSNIFKTAAAYIACGIDTEKSIIF
SQSTVSGHAELGWLLGCYTPIGWLNRMTQFKDKAGSDKQKASLGLYSYPVLMAADILLYQTKYVPVGDDQKQHLELARDI
ASAFNNHYKLDYFIVPEILTLDCTSRIMSLRDGTSKMSKSDPSEHSCINLDDTDDLIVKKIEKAKTDSILGFATLKCRPE
ISNLINIYSVLSNVNAEKVCEEVNKHDMKHFKKELADLIISVISPIREKMNDLLKDQFHLHEILKKGTKKAAEIATHNIK
EIKDIIGFAQYL

Sequences:

>Translated_332_residues
MTSIVFSGIQPSGVLHLGNYLGAIKQWIDLQDKYKSLFCIVDLHAITANKLPASELKSNIFKTAAAYIACGIDTEKSIIF
SQSTVSGHAELGWLLGCYTPIGWLNRMTQFKDKAGSDKQKASLGLYSYPVLMAADILLYQTKYVPVGDDQKQHLELARDI
ASAFNNHYKLDYFIVPEILTLDCTSRIMSLRDGTSKMSKSDPSEHSCINLDDTDDLIVKKIEKAKTDSILGFATLKCRPE
ISNLINIYSVLSNVNAEKVCEEVNKHDMKHFKKELADLIISVISPIREKMNDLLKDQFHLHEILKKGTKKAAEIATHNIK
EIKDIIGFAQYL
>Mature_331_residues
TSIVFSGIQPSGVLHLGNYLGAIKQWIDLQDKYKSLFCIVDLHAITANKLPASELKSNIFKTAAAYIACGIDTEKSIIFS
QSTVSGHAELGWLLGCYTPIGWLNRMTQFKDKAGSDKQKASLGLYSYPVLMAADILLYQTKYVPVGDDQKQHLELARDIA
SAFNNHYKLDYFIVPEILTLDCTSRIMSLRDGTSKMSKSDPSEHSCINLDDTDDLIVKKIEKAKTDSILGFATLKCRPEI
SNLINIYSVLSNVNAEKVCEEVNKHDMKHFKKELADLIISVISPIREKMNDLLKDQFHLHEILKKGTKKAAEIATHNIKE
IKDIIGFAQYL

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7710154, Length=327, Percent_Identity=42.2018348623853, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI41352700, Length=181, Percent_Identity=51.3812154696133, Blast_Score=191, Evalue=8e-49,
Organism=Escherichia coli, GI1789786, Length=329, Percent_Identity=44.6808510638298, Blast_Score=283, Evalue=9e-78,
Organism=Caenorhabditis elegans, GI71982800, Length=333, Percent_Identity=36.3363363363363, Blast_Score=225, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI71982793, Length=333, Percent_Identity=36.3363363363363, Blast_Score=223, Evalue=8e-59,
Organism=Saccharomyces cerevisiae, GI6320474, Length=346, Percent_Identity=41.3294797687861, Blast_Score=248, Evalue=8e-67,
Organism=Drosophila melanogaster, GI24666151, Length=328, Percent_Identity=40.5487804878049, Blast_Score=244, Evalue=4e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.2 [H]

Molecular weight: Translated: 37203; Mature: 37072

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIVFSGIQPSGVLHLGNYLGAIKQWIDLQDKYKSLFCIVDLHAITANKLPASELKSNI
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHCCCCCHHHHHHHH
FKTAAAYIACGIDTEKSIIFSQSTVSGHAELGWLLGCYTPIGWLNRMTQFKDKAGSDKQK
HHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ASLGLYSYPVLMAADILLYQTKYVPVGDDQKQHLELARDIASAFNNHYKLDYFIVPEILT
HHCCHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCHHHH
LDCTSRIMSLRDGTSKMSKSDPSEHSCINLDDTDDLIVKKIEKAKTDSILGFATLKCRPE
HHHHHHHHHHHCCHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHCCCHHEEEEEECCHH
ISNLINIYSVLSNVNAEKVCEEVNKHDMKHFKKELADLIISVISPIREKMNDLLKDQFHL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEILKKGTKKAAEIATHNIKEIKDIIGFAQYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSIVFSGIQPSGVLHLGNYLGAIKQWIDLQDKYKSLFCIVDLHAITANKLPASELKSNI
CCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHCCCCCHHHHHHHH
FKTAAAYIACGIDTEKSIIFSQSTVSGHAELGWLLGCYTPIGWLNRMTQFKDKAGSDKQK
HHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ASLGLYSYPVLMAADILLYQTKYVPVGDDQKQHLELARDIASAFNNHYKLDYFIVPEILT
HHCCHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCHHHH
LDCTSRIMSLRDGTSKMSKSDPSEHSCINLDDTDDLIVKKIEKAKTDSILGFATLKCRPE
HHHHHHHHHHHCCHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHCCCHHEEEEEECCHH
ISNLINIYSVLSNVNAEKVCEEVNKHDMKHFKKELADLIISVISPIREKMNDLLKDQFHL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEILKKGTKKAAEIATHNIKEIKDIIGFAQYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]