Click here to switch to the map view.

The map label for this gene is nuoE [H]

Identifier: 225630491

GI number: 225630491

Start: 803773

End: 804273

Strand: Reverse

Name: nuoE [H]

Synonym: WRi_007410

Alternate gene names: 225630491

Gene position: 804273-803773 (Counterclockwise)

Preceding gene: 225630493

Following gene: 225630490

Centisome position: 55.63

GC content: 34.53

Gene sequence:

>501_bases
ATGATAGAAAAAGAAGAGCAGTTTAGTTTTACATCAGAAAACCTCAAGAAAGCGGGAAAGTTTATAGAGATGTATCCCAA
AGGTAGAGAAGGTAGTGCTGTTATGCCTTTACTATATCTGGTTCAAGAGCAATGCGGATGGGTTTCTGAATCTGCTATGC
GTTATGTTGCTGATATGCTGCATATTCCACATATTCGTGTATATGAAGTGGCAAATTTTTACACCATGTATAATTTAAAA
CCAGTAGGCAAATATCTAATACAAATTTGTAGAACAACTCCTTGCTGGTTGTGCAATAGTGAAGAAGTTTTAAATACCTT
TAAAAAAAAACTTGGAATCAATATCGGTGAGACTACTAAAGATAATTTGTTCACTTTAAAAGAAGTTGAATGCCTAGGTG
CATGCATTAATGCTCCCGTTGTGCAGATTAATAATGACTTCTATGAAAATCTTACTCCTGAGAAAGTAGAAAACATCATA
GCAGAACTTTCAGGCAAATAG

Upstream 100 bases:

>100_bases
GAAGAGGGCTAAGAAGCTTTATGCTATTTCTCAAAAAACCAACAATTGCAATCATTGGTTCTACAGGTATAATCTGTTAA
AGTTTACACGAGTCTCATGA

Downstream 100 bases:

>100_bases
ATGAAAGAAGAATTTGCATTCAAGATAATCAAGCAATCAGGTTCTGCAAGAGTTGGCATTATTAAAACTCCAAATGGAAA
CATAGAAACACCAGCATTTA

Product: NADH dehydrogenase subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MIEKEEQFSFTSENLKKAGKFIEMYPKGREGSAVMPLLYLVQEQCGWVSESAMRYVADMLHIPHIRVYEVANFYTMYNLK
PVGKYLIQICRTTPCWLCNSEEVLNTFKKKLGINIGETTKDNLFTLKEVECLGACINAPVVQINNDFYENLTPEKVENII
AELSGK

Sequences:

>Translated_166_residues
MIEKEEQFSFTSENLKKAGKFIEMYPKGREGSAVMPLLYLVQEQCGWVSESAMRYVADMLHIPHIRVYEVANFYTMYNLK
PVGKYLIQICRTTPCWLCNSEEVLNTFKKKLGINIGETTKDNLFTLKEVECLGACINAPVVQINNDFYENLTPEKVENII
AELSGK
>Mature_166_residues
MIEKEEQFSFTSENLKKAGKFIEMYPKGREGSAVMPLLYLVQEQCGWVSESAMRYVADMLHIPHIRVYEVANFYTMYNLK
PVGKYLIQICRTTPCWLCNSEEVLNTFKKKLGINIGETTKDNLFTLKEVECLGACINAPVVQINNDFYENLTPEKVENII
AELSGK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=163, Percent_Identity=57.6687116564417, Blast_Score=198, Evalue=2e-51,
Organism=Escherichia coli, GI1788621, Length=138, Percent_Identity=35.5072463768116, Blast_Score=95, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17561328, Length=157, Percent_Identity=54.7770700636943, Blast_Score=197, Evalue=3e-51,
Organism=Drosophila melanogaster, GI18859877, Length=156, Percent_Identity=54.4871794871795, Blast_Score=193, Evalue=5e-50,
Organism=Drosophila melanogaster, GI21355353, Length=162, Percent_Identity=41.358024691358, Blast_Score=149, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 19031; Mature: 19031

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEKEEQFSFTSENLKKAGKFIEMYPKGREGSAVMPLLYLVQEQCGWVSESAMRYVADML
CCCCCHHHCCCHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
HIPHIRVYEVANFYTMYNLKPVGKYLIQICRTTPCWLCNSEEVLNTFKKKLGINIGETTK
HCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCCCCC
DNLFTLKEVECLGACINAPVVQINNDFYENLTPEKVENIIAELSGK
CCCCHHHHHHHHHHHHCCCEEEECCHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MIEKEEQFSFTSENLKKAGKFIEMYPKGREGSAVMPLLYLVQEQCGWVSESAMRYVADML
CCCCCHHHCCCHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
HIPHIRVYEVANFYTMYNLKPVGKYLIQICRTTPCWLCNSEEVLNTFKKKLGINIGETTK
HCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCCCCC
DNLFTLKEVECLGACINAPVVQINNDFYENLTPEKVENIIAELSGK
CCCCHHHHHHHHHHHHCCCEEEECCHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA