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The map label for this gene is tgt [H]

Identifier: 225630490

GI number: 225630490

Start: 802555

End: 803772

Strand: Reverse

Name: tgt [H]

Synonym: WRi_007400

Alternate gene names: 225630490

Gene position: 803772-802555 (Counterclockwise)

Preceding gene: 225630491

Following gene: 225630489

Centisome position: 55.59

GC content: 36.78

Gene sequence:

>1218_bases
ATGAAAGAAGAATTTGCATTCAAGATAATCAAGCAATCAGGTTCTGCAAGAGTTGGCATTATTAAAACTCCAAATGGAAA
CATAGAAACACCAGCATTTATATTTTGTGCAACCAAAGCTGCTATTAAAGCTGCAGATATCGAGAGAATCAGAGAAGAAG
GTACTCAGATAATACTTTCCAACACCTATCACCTAATGCTTCAACCAGGAGAGAATACTGTTGCAAAACTTGGTGGTTTG
CACAAGATGATGGGATGGAATGGGCCAATGCTAACTGACTCAGGTGGATATCAGATATTTAGCTTAGGCCACGGATCAGT
TTCGGAAGAGATAAAAGGAATAAGAAAAAAACAAAAAACTTTGATCAAAATTAATGAGGATGGGGCAATTTTTCGTTCTT
ACATCAATGGCAAAACTTATTGCTTAACTCCTGAAAAGTCTATACAAATTCAACAAAAATTAGGTGCAGATTTAATCCTA
GTTTTAGATGAATGCACTCCATTTCACGTAAGCAAAGAATACACAGCAAAATCAATGCTCATGAGCCACAGATGGGCTGA
ACGTTCTTTAAATGAATTTGAAAAAAACAATAATGGCAAGCAGGCACTGTATGGAATTAGTCAAGGCGGAATATATCACG
ATCTGCGTAGAGAAAGTTGTGATTTTATCAACAATTTACCATTTTTCGGCCAAGCAATAGGTGGATCACTTGGTCAAAGT
AAAGAGCAGATGTACGATGTAGTTTCTTTCACTATGGACCACTTGAAAAAAGACAGGCCTACTCATTTGCTTGGTATCGG
TGGGATTGTGGACATCTTTCATGGAGTAAGTCTTGGAATTGACACGTTTGATTGTGTGCATCCAACTCGTTTGGCAAGGC
ATGGTGGTGCGCTTATTAAAGTAAAAAATAGAGATTCTATTTCTTCAAAGTGCAAAGAGCATATCAATTTGCGAAATCAA
CAATTCGAGCTGGACGATAACCCAATTGAAAGTGACTGTTTATGTTTTACCTGCAGAAAACATTCAAGAGCTTATATACA
CCATTTACTGAAAGCTAAAGAGCTCCTGGCTTACACGCTCGTTACCATTCATAATATTTTCTTTATGAATAAGTTAATGG
CATCAATAAGGCAGGCAATATTAGATGATAGGCTAGATCAGGAGAAAAATAATTGGATTAGTGAAATACCATTGCACTTT
GATTTGGCCTCTCTTTAA

Upstream 100 bases:

>100_bases
ATGCATTAATGCTCCCGTTGTGCAGATTAATAATGACTTCTATGAAAATCTTACTCCTGAGAAAGTAGAAAACATCATAG
CAGAACTTTCAGGCAAATAG

Downstream 100 bases:

>100_bases
GTCTTTTCTTCATTAGACTTCTTGCATAACCATATAACGAACTTTTATTGGAAATAGAAACGAAAAAACTTACTTGACAA
ACTCCGCCAGCCCCCTTATC

Product: Queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MKEEFAFKIIKQSGSARVGIIKTPNGNIETPAFIFCATKAAIKAADIERIREEGTQIILSNTYHLMLQPGENTVAKLGGL
HKMMGWNGPMLTDSGGYQIFSLGHGSVSEEIKGIRKKQKTLIKINEDGAIFRSYINGKTYCLTPEKSIQIQQKLGADLIL
VLDECTPFHVSKEYTAKSMLMSHRWAERSLNEFEKNNNGKQALYGISQGGIYHDLRRESCDFINNLPFFGQAIGGSLGQS
KEQMYDVVSFTMDHLKKDRPTHLLGIGGIVDIFHGVSLGIDTFDCVHPTRLARHGGALIKVKNRDSISSKCKEHINLRNQ
QFELDDNPIESDCLCFTCRKHSRAYIHHLLKAKELLAYTLVTIHNIFFMNKLMASIRQAILDDRLDQEKNNWISEIPLHF
DLASL

Sequences:

>Translated_405_residues
MKEEFAFKIIKQSGSARVGIIKTPNGNIETPAFIFCATKAAIKAADIERIREEGTQIILSNTYHLMLQPGENTVAKLGGL
HKMMGWNGPMLTDSGGYQIFSLGHGSVSEEIKGIRKKQKTLIKINEDGAIFRSYINGKTYCLTPEKSIQIQQKLGADLIL
VLDECTPFHVSKEYTAKSMLMSHRWAERSLNEFEKNNNGKQALYGISQGGIYHDLRRESCDFINNLPFFGQAIGGSLGQS
KEQMYDVVSFTMDHLKKDRPTHLLGIGGIVDIFHGVSLGIDTFDCVHPTRLARHGGALIKVKNRDSISSKCKEHINLRNQ
QFELDDNPIESDCLCFTCRKHSRAYIHHLLKAKELLAYTLVTIHNIFFMNKLMASIRQAILDDRLDQEKNNWISEIPLHF
DLASL
>Mature_405_residues
MKEEFAFKIIKQSGSARVGIIKTPNGNIETPAFIFCATKAAIKAADIERIREEGTQIILSNTYHLMLQPGENTVAKLGGL
HKMMGWNGPMLTDSGGYQIFSLGHGSVSEEIKGIRKKQKTLIKINEDGAIFRSYINGKTYCLTPEKSIQIQQKLGADLIL
VLDECTPFHVSKEYTAKSMLMSHRWAERSLNEFEKNNNGKQALYGISQGGIYHDLRRESCDFINNLPFFGQAIGGSLGQS
KEQMYDVVSFTMDHLKKDRPTHLLGIGGIVDIFHGVSLGIDTFDCVHPTRLARHGGALIKVKNRDSISSKCKEHINLRNQ
QFELDDNPIESDCLCFTCRKHSRAYIHHLLKAKELLAYTLVTIHNIFFMNKLMASIRQAILDDRLDQEKNNWISEIPLHF
DLASL

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=371, Percent_Identity=37.7358490566038, Blast_Score=255, Evalue=5e-68,
Organism=Homo sapiens, GI13375872, Length=222, Percent_Identity=26.5765765765766, Blast_Score=83, Evalue=6e-16,
Organism=Escherichia coli, GI1786607, Length=381, Percent_Identity=39.6325459317585, Blast_Score=292, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI17544680, Length=382, Percent_Identity=34.8167539267016, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI25146502, Length=233, Percent_Identity=27.0386266094421, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920510, Length=386, Percent_Identity=34.7150259067358, Blast_Score=241, Evalue=5e-64,
Organism=Drosophila melanogaster, GI21355311, Length=148, Percent_Identity=29.0540540540541, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 45641; Mature: 45641

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEEFAFKIIKQSGSARVGIIKTPNGNIETPAFIFCATKAAIKAADIERIREEGTQIILS
CCCHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCEEEEE
NTYHLMLQPGENTVAKLGGLHKMMGWNGPMLTDSGGYQIFSLGHGSVSEEIKGIRKKQKT
CCEEEEEECCCHHHHHHCCHHHHHCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHCCE
LIKINEDGAIFRSYINGKTYCLTPEKSIQIQQKLGADLILVLDECTPFHVSKEYTAKSML
EEEECCCCHHHHHHHCCCEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHH
MSHRWAERSLNEFEKNNNGKQALYGISQGGIYHDLRRESCDFINNLPFFGQAIGGSLGQS
HHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCH
KEQMYDVVSFTMDHLKKDRPTHLLGIGGIVDIFHGVSLGIDTFDCVHPTRLARHGGALIK
HHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCHHCCCCCHHHCCHHHHHHCCCCEEE
VKNRDSISSKCKEHINLRNQQFELDDNPIESDCLCFTCRKHSRAYIHHLLKAKELLAYTL
ECCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
VTIHNIFFMNKLMASIRQAILDDRLDQEKNNWISEIPLHFDLASL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEECCCC
>Mature Secondary Structure
MKEEFAFKIIKQSGSARVGIIKTPNGNIETPAFIFCATKAAIKAADIERIREEGTQIILS
CCCHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCEEEEE
NTYHLMLQPGENTVAKLGGLHKMMGWNGPMLTDSGGYQIFSLGHGSVSEEIKGIRKKQKT
CCEEEEEECCCHHHHHHCCHHHHHCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHCCE
LIKINEDGAIFRSYINGKTYCLTPEKSIQIQQKLGADLILVLDECTPFHVSKEYTAKSML
EEEECCCCHHHHHHHCCCEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHH
MSHRWAERSLNEFEKNNNGKQALYGISQGGIYHDLRRESCDFINNLPFFGQAIGGSLGQS
HHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCH
KEQMYDVVSFTMDHLKKDRPTHLLGIGGIVDIFHGVSLGIDTFDCVHPTRLARHGGALIK
HHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCHHCCCCCHHHCCHHHHHHCCCCEEE
VKNRDSISSKCKEHINLRNQQFELDDNPIESDCLCFTCRKHSRAYIHHLLKAKELLAYTL
ECCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
VTIHNIFFMNKLMASIRQAILDDRLDQEKNNWISEIPLHFDLASL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA