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The map label for this gene is mrdA [H]

Identifier: 225630425

GI number: 225630425

Start: 712437

End: 714011

Strand: Reverse

Name: mrdA [H]

Synonym: WRi_006600

Alternate gene names: 225630425

Gene position: 714011-712437 (Counterclockwise)

Preceding gene: 225630426

Following gene: 225630424

Centisome position: 49.38

GC content: 37.02

Gene sequence:

>1575_bases
ATGTGGACAAAAAATAAAGTCTTTAACCGCAGAGCATTCATATTAGGTGGTATTCAGCTTACCATTTCCGCGGTTTTTAG
TTGCAGGTTATATAATTTACAAATACGAAACAGACAAAAGTACGAAGCACTATCTAATAGTAATAGAATAAGAGTTGCTA
CTATTATGCCTAAGCGGGGCAAAATTTTAGATAGGAATAGCATTGAACTTGCGGTAAACAAAATTTCGTATGTTGTTTTG
TTTGATGGTTCTGGTAAGGAAGTTGATTTGCAGACATTATCAGAAGTCGAGTCTAAAATAGCAAAATCGTCAGAAAAAAT
AACTGCTCTTTATAAACGTTATTACCCGTTTGGTTCAATGTGTTCTCATGTAATCGGATATACAAAAAGACAGCAAGGCA
TAAGCGAAGTAGGAATCAGCGGTATTGAATATACATATGATCATATATTGAAAGGCAAGTCAGGAAAATCTGAGCAGGAA
ATAAATTCTAAAAAACGCGTCATAAAAGAATTATCAAGCATACCACAACAAGACGGACAAGATGTACAACTGACAATTGA
TATTAATCTGCAAGAGAAAACCGCAGAGGTATTTAAAGATCACCAAGGCTCCGCAGTGGTAATTGATGTAAATAATGGAG
AAATTTTAGCATTATATAATTCACCTTCTTACGATAATAATCTTTTTGCTAGCAGGCTATCAAATGAGACTTGGGAGAGC
TTGAATGCTCCTTCATTACCGCTTGTGAATCGTGCGTTATCATATCAAATTCCACCTGGTTCAATATTTAAAGTAATAGT
TGCGCTTGCGGGTTTAAAAGACGGGATAATAACACCAGAAGAGAAATTCTCGTGCAAGGGCTATATGAAAATAGGTGAGC
GGAAATTTCGTTGTCTGAAAAGCAAAGTCCATGGGTATGTATCTTTAAATGAGGCAATGGCATTATCATGCAACACTTAC
TTTTATAATATAGGGAAAAAAATAAGTGTAGACTCTCTAGTAGAAATGGCCAGAAAATTTGGTATTGGAAGTGGGCCACT
GATTGGAACGTTTAAAGAAGAAGCTCCAGGGTTGTTGCCAGATAGAGATTGGCGTACACGAAAGCTATATTCGCAGTGGT
ATTTAGGTGACACTATCAACTTAGTTATAGGACAAGGGTATATGCTTACAACACCACTACAGCTTGCAGTTCTTGCAGCG
AGGATTGCAACAGGAAAAGAGGTAATTCCCCGCATTAAGATGAATGAAACGATACAAGATTTTCCCGATATTGATGTGGA
TTGTGAGCATCTTAGCATAGTTCGAAAAGCTATGTTTGATGTGGTGAATTCTAAAACTGGAACCTATAAGAAAGGGCTAA
GCGGTATACAAATTGCCGGTAAAACCGGTACACCAGAGATAAACTCTAAGGGTGAAAGTCATAAGTTATTTATCGCTTAT
GGCCCTTACCATAACCCGCGCTATGCAATCTCTGTGTTTATAGAACACGGCAAAGCCCCACGCCAAGATGTTGCTATAGC
TAATGAAATATTTCAGTATATGCTTGAAACTATGAGTATTAAACTTCTTGCATAA

Upstream 100 bases:

>100_bases
TTGTGACCTTAAGTGAGTGGGAAAAAAGGGTTTGTAATACGGTAAAAGCCAGAAGTGTAACAATAAAAGAGGGGAAAAAA
GTGCGTTTTGAAGGAATGTT

Downstream 100 bases:

>100_bases
CCATATAACAAACTTTTATTGGGAATAGAAACGAAAAAACTTACTTGACACCCTTCGCCAGCGCCCTTATCATGATAGTG
AAGCTATTGTATTTGCTTTC

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MWTKNKVFNRRAFILGGIQLTISAVFSCRLYNLQIRNRQKYEALSNSNRIRVATIMPKRGKILDRNSIELAVNKISYVVL
FDGSGKEVDLQTLSEVESKIAKSSEKITALYKRYYPFGSMCSHVIGYTKRQQGISEVGISGIEYTYDHILKGKSGKSEQE
INSKKRVIKELSSIPQQDGQDVQLTIDINLQEKTAEVFKDHQGSAVVIDVNNGEILALYNSPSYDNNLFASRLSNETWES
LNAPSLPLVNRALSYQIPPGSIFKVIVALAGLKDGIITPEEKFSCKGYMKIGERKFRCLKSKVHGYVSLNEAMALSCNTY
FYNIGKKISVDSLVEMARKFGIGSGPLIGTFKEEAPGLLPDRDWRTRKLYSQWYLGDTINLVIGQGYMLTTPLQLAVLAA
RIATGKEVIPRIKMNETIQDFPDIDVDCEHLSIVRKAMFDVVNSKTGTYKKGLSGIQIAGKTGTPEINSKGESHKLFIAY
GPYHNPRYAISVFIEHGKAPRQDVAIANEIFQYMLETMSIKLLA

Sequences:

>Translated_524_residues
MWTKNKVFNRRAFILGGIQLTISAVFSCRLYNLQIRNRQKYEALSNSNRIRVATIMPKRGKILDRNSIELAVNKISYVVL
FDGSGKEVDLQTLSEVESKIAKSSEKITALYKRYYPFGSMCSHVIGYTKRQQGISEVGISGIEYTYDHILKGKSGKSEQE
INSKKRVIKELSSIPQQDGQDVQLTIDINLQEKTAEVFKDHQGSAVVIDVNNGEILALYNSPSYDNNLFASRLSNETWES
LNAPSLPLVNRALSYQIPPGSIFKVIVALAGLKDGIITPEEKFSCKGYMKIGERKFRCLKSKVHGYVSLNEAMALSCNTY
FYNIGKKISVDSLVEMARKFGIGSGPLIGTFKEEAPGLLPDRDWRTRKLYSQWYLGDTINLVIGQGYMLTTPLQLAVLAA
RIATGKEVIPRIKMNETIQDFPDIDVDCEHLSIVRKAMFDVVNSKTGTYKKGLSGIQIAGKTGTPEINSKGESHKLFIAY
GPYHNPRYAISVFIEHGKAPRQDVAIANEIFQYMLETMSIKLLA
>Mature_524_residues
MWTKNKVFNRRAFILGGIQLTISAVFSCRLYNLQIRNRQKYEALSNSNRIRVATIMPKRGKILDRNSIELAVNKISYVVL
FDGSGKEVDLQTLSEVESKIAKSSEKITALYKRYYPFGSMCSHVIGYTKRQQGISEVGISGIEYTYDHILKGKSGKSEQE
INSKKRVIKELSSIPQQDGQDVQLTIDINLQEKTAEVFKDHQGSAVVIDVNNGEILALYNSPSYDNNLFASRLSNETWES
LNAPSLPLVNRALSYQIPPGSIFKVIVALAGLKDGIITPEEKFSCKGYMKIGERKFRCLKSKVHGYVSLNEAMALSCNTY
FYNIGKKISVDSLVEMARKFGIGSGPLIGTFKEEAPGLLPDRDWRTRKLYSQWYLGDTINLVIGQGYMLTTPLQLAVLAA
RIATGKEVIPRIKMNETIQDFPDIDVDCEHLSIVRKAMFDVVNSKTGTYKKGLSGIQIAGKTGTPEINSKGESHKLFIAY
GPYHNPRYAISVFIEHGKAPRQDVAIANEIFQYMLETMSIKLLA

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=586, Percent_Identity=30.3754266211604, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1786272, Length=431, Percent_Identity=28.3062645011601, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 58677; Mature: 58677

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWTKNKVFNRRAFILGGIQLTISAVFSCRLYNLQIRNRQKYEALSNSNRIRVATIMPKRG
CCCCCCCCCCEEEEEECHHHHHHHHHHEEEEEEEECCCHHHHHHCCCCCEEEEEECCCCC
KILDRNSIELAVNKISYVVLFDGSGKEVDLQTLSEVESKIAKSSEKITALYKRYYPFGSM
CEECCCCCEEEEEEEEEEEEECCCCCEEHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHH
CSHVIGYTKRQQGISEVGISGIEYTYDHILKGKSGKSEQEINSKKRVIKELSSIPQQDGQ
HHHHHHHHHHHCCHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
DVQLTIDINLQEKTAEVFKDHQGSAVVIDVNNGEILALYNSPSYDNNLFASRLSNETWES
EEEEEEEECCCHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHCCHHHHH
LNAPSLPLVNRALSYQIPPGSIFKVIVALAGLKDGIITPEEKFSCKGYMKIGERKFRCLK
CCCCCCHHHHHHHEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SKVHGYVSLNEAMALSCNTYFYNIGKKISVDSLVEMARKFGIGSGPLIGTFKEEAPGLLP
HHHCCEEEECCEEEEEECEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEECHHHCCCCCC
DRDWRTRKLYSQWYLGDTINLVIGQGYMLTTPLQLAVLAARIATGKEVIPRIKMNETIQD
CCCCHHHHHHHHHHCCCEEEEEEECCEEEECHHHHHHHHHHHHCCHHHHCCCCCCCHHHH
FPDIDVDCEHLSIVRKAMFDVVNSKTGTYKKGLSGIQIAGKTGTPEINSKGESHKLFIAY
CCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCCCCCCCCCCCCCEEEEEEE
GPYHNPRYAISVFIEHGKAPRQDVAIANEIFQYMLETMSIKLLA
CCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCEEEEC
>Mature Secondary Structure
MWTKNKVFNRRAFILGGIQLTISAVFSCRLYNLQIRNRQKYEALSNSNRIRVATIMPKRG
CCCCCCCCCCEEEEEECHHHHHHHHHHEEEEEEEECCCHHHHHHCCCCCEEEEEECCCCC
KILDRNSIELAVNKISYVVLFDGSGKEVDLQTLSEVESKIAKSSEKITALYKRYYPFGSM
CEECCCCCEEEEEEEEEEEEECCCCCEEHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHH
CSHVIGYTKRQQGISEVGISGIEYTYDHILKGKSGKSEQEINSKKRVIKELSSIPQQDGQ
HHHHHHHHHHHCCHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
DVQLTIDINLQEKTAEVFKDHQGSAVVIDVNNGEILALYNSPSYDNNLFASRLSNETWES
EEEEEEEECCCHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHCCHHHHH
LNAPSLPLVNRALSYQIPPGSIFKVIVALAGLKDGIITPEEKFSCKGYMKIGERKFRCLK
CCCCCCHHHHHHHEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SKVHGYVSLNEAMALSCNTYFYNIGKKISVDSLVEMARKFGIGSGPLIGTFKEEAPGLLP
HHHCCEEEECCEEEEEECEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEECHHHCCCCCC
DRDWRTRKLYSQWYLGDTINLVIGQGYMLTTPLQLAVLAARIATGKEVIPRIKMNETIQD
CCCCHHHHHHHHHHCCCEEEEEEECCEEEECHHHHHHHHHHHHCCHHHHCCCCCCCHHHH
FPDIDVDCEHLSIVRKAMFDVVNSKTGTYKKGLSGIQIAGKTGTPEINSKGESHKLFIAY
CCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCCCCCCCCCCCCCEEEEEEE
GPYHNPRYAISVFIEHGKAPRQDVAIANEIFQYMLETMSIKLLA
CCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]