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The map label for this gene is tsaA [H]

Identifier: 225630373

GI number: 225630373

Start: 645141

End: 645740

Strand: Reverse

Name: tsaA [H]

Synonym: WRi_005990

Alternate gene names: 225630373

Gene position: 645740-645141 (Counterclockwise)

Preceding gene: 225630374

Following gene: 225630372

Centisome position: 44.66

GC content: 35.5

Gene sequence:

>600_bases
ATGAATCTTGTAACAAAATCTGCTATCGATTTTACTGCTTCATCTGTTCTCTCTGACGGAGAAATTGTTGATGATTTCTG
TCTGAGTAAGCGCATAAAAAATAAGTATGCTGTCCTTTTTTTCTATCCACTTGATTTTACTTTTGTTTGCCCAACTGAAT
TGATATCGCTCAATAATAGAATAAATGAGTTTGCAAAGCGTGGTGTTGAAGTGATAGGAATAAGTGTAGATTCAAAATTT
TCACATTATAAATGGCGAAACACTCCAGTTAATGATGGTGGTATTGGAGAGATTAGCTACACTTTAGTGTCTGATATCAA
AAAGTCTATATCAAGAGACTATGGGGTCTTATATGATGACTCAATTGCGCTGAGAGCAACTTTCGTTATTGATGATAAAT
TTATTGTACGTCATCAATCGATAAATGATTTGCCTTTAGGGCGTAATATCGATGAATTCGTTAGAATCGTTGATGCAATT
AAACATAATGAAGAGCATGGTGAGGTGTGTCCAGCAGGGTGGAAAAAAGGTAAGCCAGCAATGCAGGCAAGCGATGAAGG
AGTAGCTGATTATCTAAACTCATACAGTGAAGAATTATAA

Upstream 100 bases:

>100_bases
GCTATTTTAGGAAGTTTTTATAGGACTAAAACCAAAGAAAACTTGCATATAAGTTTATCAAGATGTAAAATTAAAGTTAT
TTTAAAAGAATAGGTTAATT

Downstream 100 bases:

>100_bases
ATTTAGTACAAAAGTTCTTATTATTGGATCTGGGGCAGCGGGTTATGCTGCTGCTATATATGCAGCACGTGCAAACTTAG
AGCCAATTGTAGTAACGGGA

Product: antioxidant, AhpC/Tsa family

Products: NA

Alternate protein names: 26 kDa antigen; Thioredoxin reductase [H]

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MNLVTKSAIDFTASSVLSDGEIVDDFCLSKRIKNKYAVLFFYPLDFTFVCPTELISLNNRINEFAKRGVEVIGISVDSKF
SHYKWRNTPVNDGGIGEISYTLVSDIKKSISRDYGVLYDDSIALRATFVIDDKFIVRHQSINDLPLGRNIDEFVRIVDAI
KHNEEHGEVCPAGWKKGKPAMQASDEGVADYLNSYSEEL

Sequences:

>Translated_199_residues
MNLVTKSAIDFTASSVLSDGEIVDDFCLSKRIKNKYAVLFFYPLDFTFVCPTELISLNNRINEFAKRGVEVIGISVDSKF
SHYKWRNTPVNDGGIGEISYTLVSDIKKSISRDYGVLYDDSIALRATFVIDDKFIVRHQSINDLPLGRNIDEFVRIVDAI
KHNEEHGEVCPAGWKKGKPAMQASDEGVADYLNSYSEEL
>Mature_199_residues
MNLVTKSAIDFTASSVLSDGEIVDDFCLSKRIKNKYAVLFFYPLDFTFVCPTELISLNNRINEFAKRGVEVIGISVDSKF
SHYKWRNTPVNDGGIGEISYTLVSDIKKSISRDYGVLYDDSIALRATFVIDDKFIVRHQSINDLPLGRNIDEFVRIVDAI
KHNEEHGEVCPAGWKKGKPAMQASDEGVADYLNSYSEEL

Specific function: Directly Reduces Organic Hydroperoxides In Its Reduced Dithiol Form. [C]

COG id: COG0450

COG function: function code O; Peroxiredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Homo sapiens, GI4505591, Length=188, Percent_Identity=51.063829787234, Blast_Score=194, Evalue=5e-50,
Organism=Homo sapiens, GI32455266, Length=188, Percent_Identity=51.063829787234, Blast_Score=194, Evalue=5e-50,
Organism=Homo sapiens, GI32455264, Length=188, Percent_Identity=51.063829787234, Blast_Score=194, Evalue=5e-50,
Organism=Homo sapiens, GI32189392, Length=194, Percent_Identity=47.4226804123711, Blast_Score=191, Evalue=3e-49,
Organism=Homo sapiens, GI5802974, Length=199, Percent_Identity=45.7286432160804, Blast_Score=188, Evalue=2e-48,
Organism=Homo sapiens, GI32483377, Length=176, Percent_Identity=47.7272727272727, Blast_Score=185, Evalue=2e-47,
Organism=Homo sapiens, GI5453549, Length=162, Percent_Identity=50, Blast_Score=177, Evalue=4e-45,
Organism=Homo sapiens, GI33188454, Length=82, Percent_Identity=54.8780487804878, Blast_Score=87, Evalue=7e-18,
Organism=Homo sapiens, GI4758638, Length=160, Percent_Identity=27.5, Blast_Score=64, Evalue=7e-11,
Organism=Escherichia coli, GI1786822, Length=186, Percent_Identity=32.258064516129, Blast_Score=114, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI193204376, Length=189, Percent_Identity=48.6772486772487, Blast_Score=173, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI32565831, Length=189, Percent_Identity=48.6772486772487, Blast_Score=173, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17554494, Length=162, Percent_Identity=46.2962962962963, Blast_Score=167, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI25153706, Length=161, Percent_Identity=27.9503105590062, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323613, Length=192, Percent_Identity=41.6666666666667, Blast_Score=159, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6320661, Length=188, Percent_Identity=42.0212765957447, Blast_Score=147, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6319407, Length=169, Percent_Identity=29.585798816568, Blast_Score=79, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24656348, Length=191, Percent_Identity=46.5968586387435, Blast_Score=181, Evalue=2e-46,
Organism=Drosophila melanogaster, GI17864676, Length=191, Percent_Identity=46.5968586387435, Blast_Score=181, Evalue=2e-46,
Organism=Drosophila melanogaster, GI17738015, Length=143, Percent_Identity=55.2447552447552, Blast_Score=174, Evalue=3e-44,
Organism=Drosophila melanogaster, GI17157991, Length=197, Percent_Identity=43.6548223350254, Blast_Score=173, Evalue=7e-44,
Organism=Drosophila melanogaster, GI24641739, Length=197, Percent_Identity=43.6548223350254, Blast_Score=173, Evalue=7e-44,
Organism=Drosophila melanogaster, GI21357347, Length=190, Percent_Identity=41.5789473684211, Blast_Score=165, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24581278, Length=157, Percent_Identity=33.7579617834395, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17975518, Length=162, Percent_Identity=31.4814814814815, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24652436, Length=162, Percent_Identity=31.4814814814815, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24652434, Length=162, Percent_Identity=31.4814814814815, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2250 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 6040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1580 Molecules/Cell In: Stationary-Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000866
- InterPro:   IPR019479
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF10417 1-cysPrx_C; PF00578 AhpC-TSA [H]

EC number: =1.11.1.15 [H]

Molecular weight: Translated: 22399; Mature: 22399

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00014 ER_TARGET

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLVTKSAIDFTASSVLSDGEIVDDFCLSKRIKNKYAVLFFYPLDFTFVCPTELISLNNR
CCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEECCCEEEECHHHHHHHHHH
INEFAKRGVEVIGISVDSKFSHYKWRNTPVNDGGIGEISYTLVSDIKKSISRDYGVLYDD
HHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECC
SIALRATFVIDDKFIVRHQSINDLPLGRNIDEFVRIVDAIKHNEEHGEVCPAGWKKGKPA
CEEEEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
MQASDEGVADYLNSYSEEL
CCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNLVTKSAIDFTASSVLSDGEIVDDFCLSKRIKNKYAVLFFYPLDFTFVCPTELISLNNR
CCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEECCCEEEECHHHHHHHHHH
INEFAKRGVEVIGISVDSKFSHYKWRNTPVNDGGIGEISYTLVSDIKKSISRDYGVLYDD
HHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECC
SIALRATFVIDDKFIVRHQSINDLPLGRNIDEFVRIVDAIKHNEEHGEVCPAGWKKGKPA
CEEEEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
MQASDEGVADYLNSYSEEL
CCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682; 1987145 [H]