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The map label for this gene is hpaIM [H]

Identifier: 225630341

GI number: 225630341

Start: 598474

End: 599700

Strand: Reverse

Name: hpaIM [H]

Synonym: WRi_005640

Alternate gene names: 225630341

Gene position: 599700-598474 (Counterclockwise)

Preceding gene: 225630342

Following gene: 225630340

Centisome position: 41.48

GC content: 36.67

Gene sequence:

>1227_bases
ATGAATTTAGCAATCCACTACTATCCTGTTGAAAACCTCGTCGAATATAAGCGTAATCCTCGTAAAAATGATGATGTAGT
AAATAGAATGTGTGCTTCAATCAGGGAATTTGGCTTTCGTATACCAATAGTTGCAAAAAGCGATGGAACTGTGGTTGATG
GTCATTTAAGACTTAAAGCAGCAAGAAAACTTGGTATGGAGAGTATTCCAGTGGTCTTAAGTGATAATTTAAATGAACCA
CAAACTAAAGCTTTTCGGTTACTGGCAAATCAATCAGCTAACTGGGCAAAGTGGGACGATGAGCTTTTGAAAGTGGAAAT
TCAAGAGTTAGAAGATTTGCAGTTTGATCTTAAAATGACTGGATTTGAATTGGAAAAAGTTCAACGTTTTCTCGATGATT
TTGATGGAAAAGAAGAAGTAGAAAGAGGAGTTTTGGAGTTAATTGATAAAAAGGTAGAAATAGCAAAACCAGGGGATTTA
TGGATTTTAGGTGATCATAGAATCTATTGCGGTGATAGCTGCGTAGTTGAATCATTTAAAGCAGTATTAGATGATAAAAT
GGCAGATATTACTGTTTGTGATCCTCCATATAACGTTGATTATGGTAGTAGCCAGGAGAGAGAAGATAAAAAAATATTGA
ATGATAATCAAGGTGAAAAGTATGAGCTTTTTCTCTACGACATCTGTTCCAATATTTTAGCATATACGAAAGGAGCAATT
TACATCTGCATATCATCATCAGAGTTTTCAACGCTGCAAAAAGCATTTGAGGAAGCAGGAGGAAAATGGTCAACATTTAT
CATTTGGGCAAAGAATCATTTTACGCTAGGCAGGTCTGATTATCAAAGACAATACGAAGCAATGCTCTATGGATGGAAAA
GCGGCAATAAACGTGAGTGGCATGGAGGTAGAAATCAAAGTGATCTATGGTTTTATGATAAGCCAATACATAACTCACTG
CACCCAACAATGAAGCCAGTAGAGCTAATGGAGAAAGCAATAGTTAATAGCAGCAGACCTGGGGATACTGTACTTGATCC
ATTTAGCGGTTCTGGCAGCACACTGATTGCATGTGAGAGAACAGGAAGAATTTGCAGAACAATAGAGCTAGATTCAAAAT
TTGTAGATGTAGCGATAAAACGTTGGCAAGTATACACAGGTAGGGAAGCAATTCTTTCTGGTACTGGCAAAACTTTTGCA
CAAATTCAAGAAGAAAAACAGCAATAA

Upstream 100 bases:

>100_bases
TCAGCCAGAATGGCGGATTTGGTGGGTTCAACCCCAGGCCTTCTTTAGCGTCAGAGATATTTTGAATATTAACTTTTTAA
TTATTAAGGATTTAAATTAT

Downstream 100 bases:

>100_bases
AGGGGCAAAAGAAGTGGAAAAGATCACACAAACGGAATGGGCAAGAGAGATAGGAGTTTCAAAGCAATATGTCTGTTATT
TAGTAAAAAAAGGAATAATT

Product: DNA methylase

Products: NA

Alternate protein names: M.HpaI; Adenine-specific methyltransferase HpaI [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MNLAIHYYPVENLVEYKRNPRKNDDVVNRMCASIREFGFRIPIVAKSDGTVVDGHLRLKAARKLGMESIPVVLSDNLNEP
QTKAFRLLANQSANWAKWDDELLKVEIQELEDLQFDLKMTGFELEKVQRFLDDFDGKEEVERGVLELIDKKVEIAKPGDL
WILGDHRIYCGDSCVVESFKAVLDDKMADITVCDPPYNVDYGSSQEREDKKILNDNQGEKYELFLYDICSNILAYTKGAI
YICISSSEFSTLQKAFEEAGGKWSTFIIWAKNHFTLGRSDYQRQYEAMLYGWKSGNKREWHGGRNQSDLWFYDKPIHNSL
HPTMKPVELMEKAIVNSSRPGDTVLDPFSGSGSTLIACERTGRICRTIELDSKFVDVAIKRWQVYTGREAILSGTGKTFA
QIQEEKQQ

Sequences:

>Translated_408_residues
MNLAIHYYPVENLVEYKRNPRKNDDVVNRMCASIREFGFRIPIVAKSDGTVVDGHLRLKAARKLGMESIPVVLSDNLNEP
QTKAFRLLANQSANWAKWDDELLKVEIQELEDLQFDLKMTGFELEKVQRFLDDFDGKEEVERGVLELIDKKVEIAKPGDL
WILGDHRIYCGDSCVVESFKAVLDDKMADITVCDPPYNVDYGSSQEREDKKILNDNQGEKYELFLYDICSNILAYTKGAI
YICISSSEFSTLQKAFEEAGGKWSTFIIWAKNHFTLGRSDYQRQYEAMLYGWKSGNKREWHGGRNQSDLWFYDKPIHNSL
HPTMKPVELMEKAIVNSSRPGDTVLDPFSGSGSTLIACERTGRICRTIELDSKFVDVAIKRWQVYTGREAILSGTGKTFA
QIQEEKQQ
>Mature_408_residues
MNLAIHYYPVENLVEYKRNPRKNDDVVNRMCASIREFGFRIPIVAKSDGTVVDGHLRLKAARKLGMESIPVVLSDNLNEP
QTKAFRLLANQSANWAKWDDELLKVEIQELEDLQFDLKMTGFELEKVQRFLDDFDGKEEVERGVLELIDKKVEIAKPGDL
WILGDHRIYCGDSCVVESFKAVLDDKMADITVCDPPYNVDYGSSQEREDKKILNDNQGEKYELFLYDICSNILAYTKGAI
YICISSSEFSTLQKAFEEAGGKWSTFIIWAKNHFTLGRSDYQRQYEAMLYGWKSGNKREWHGGRNQSDLWFYDKPIHNSL
HPTMKPVELMEKAIVNSSRPGDTVLDPFSGSGSTLIACERTGRICRTIELDSKFVDVAIKRWQVYTGREAILSGTGKTFA
QIQEEKQQ

Specific function: This methylase recognizes the double-stranded sequence GTTAAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the HpaI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 46763; Mature: 46763

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLAIHYYPVENLVEYKRNPRKNDDVVNRMCASIREFGFRIPIVAKSDGTVVDGHLRLKA
CCEEEEEECHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEECCEEEHH
ARKLGMESIPVVLSDNLNEPQTKAFRLLANQSANWAKWDDELLKVEIQELEDLQFDLKMT
HHHCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEC
GFELEKVQRFLDDFDGKEEVERGVLELIDKKVEIAKPGDLWILGDHRIYCGDSCVVESFK
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHCHHEEECCCCEEEEECCEEEECCHHHHHHHH
AVLDDKMADITVCDPPYNVDYGSSQEREDKKILNDNQGEKYELFLYDICSNILAYTKGAI
HHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCCEE
YICISSSEFSTLQKAFEEAGGKWSTFIIWAKNHFTLGRSDYQRQYEAMLYGWKSGNKREW
EEEECCCHHHHHHHHHHHCCCCEEEEEEEECCCEEECCHHHHHHHHHHEEECCCCCCCCC
HGGRNQSDLWFYDKPIHNSLHPTMKPVELMEKAIVNSSRPGDTVLDPFSGSGSTLIACER
CCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEECCCCCCCCEEEEEEC
TGRICRTIELDSKFVDVAIKRWQVYTGREAILSGTGKTFAQIQEEKQQ
CCCEEEEEECCCHHHHHHHHHHEEECCCHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNLAIHYYPVENLVEYKRNPRKNDDVVNRMCASIREFGFRIPIVAKSDGTVVDGHLRLKA
CCEEEEEECHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEECCEEEHH
ARKLGMESIPVVLSDNLNEPQTKAFRLLANQSANWAKWDDELLKVEIQELEDLQFDLKMT
HHHCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEC
GFELEKVQRFLDDFDGKEEVERGVLELIDKKVEIAKPGDLWILGDHRIYCGDSCVVESFK
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHCHHEEECCCCEEEEECCEEEECCHHHHHHHH
AVLDDKMADITVCDPPYNVDYGSSQEREDKKILNDNQGEKYELFLYDICSNILAYTKGAI
HHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCCEE
YICISSSEFSTLQKAFEEAGGKWSTFIIWAKNHFTLGRSDYQRQYEAMLYGWKSGNKREW
EEEECCCHHHHHHHHHHHCCCCEEEEEEEECCCEEECCHHHHHHHHHHEEECCCCCCCCC
HGGRNQSDLWFYDKPIHNSLHPTMKPVELMEKAIVNSSRPGDTVLDPFSGSGSTLIACER
CCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEECCCCCCCCEEEEEEC
TGRICRTIELDSKFVDVAIKRWQVYTGREAILSGTGKTFAQIQEEKQQ
CCCEEEEEECCCHHHHHHHHHHEEECCCHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1542567 [H]