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The map label for this gene is carA [H]

Identifier: 225630304

GI number: 225630304

Start: 554501

End: 555592

Strand: Reverse

Name: carA [H]

Synonym: WRi_005240

Alternate gene names: 225630304

Gene position: 555592-554501 (Counterclockwise)

Preceding gene: 225630306

Following gene: 225630303

Centisome position: 38.43

GC content: 35.99

Gene sequence:

>1092_bases
GTGCAGGACGCAGTTTTAATTTTACAAGATGGTAAATGCTTTTGGGGAAAATCAATAGGTAAAAAAGGTAAGTGCATAGG
TGAGGTCTGTTTTACCACTGGTATGACCGGTTATCAGCATACTATAACTGATCCTTCTTTTGCCGATCAAATTATAACGT
TCACTTTTCCTCATATTGGTAACGTTGGAATAAACCATAAGGATAATGAAGGAAAGAAAATTTTTGCAAGTGGTGTGGTT
ATGCGTGAACTTTCTTCTATGTCCCACCCTTCTTCATATATCAGTTTAAATGATTGGTTAGAGAAAAATAACGTGATTGG
GATATCAGGAGTTGATACTAGAGCTTTAACGAGACATTTGAGAGAACATGGATCTCAAAATGGAATGATATGCTCATCGA
GTAAGGCGCATGCGTTGGACAAATTGAAGGAATACAAATCTGTAAATGGGATAGAAATAACCAATAAAGTCAGCTTGCAT
AGTAATTTTAAAAGTGACCTTAATGCAAAATATAGGGTTGTAATCGTTGATTTTGGCGTAAAAATCAGTATAGTTTCACG
CTTAATAGAACTTGGTTGTACAGTTGAATTAATCAGACCAAGCACAGGTTTTGCTCAAAAAGTGTTGAGTATGAATCCAG
ATGGGATAGTGCTTTCAAATGGTCCTGGTGATCCGCAAGAAATAGGAGAAAGTGTAGTTTCAGAAATAGACATTATTATA
AAATCTAAAATACCAATTTTTGGCATATGCATGGGCCATCAATTACTTGCGATTACTTTGGGGGCAAAGACCATCAAGAT
GGATATAGGTCACCGAGGGAGCAACCATCCAGTTTATGATGTAAGTAGTGGAAAAGTTGGGATCACTAGCCAAAATCATG
GTTTTGTTGTTGATTCAGCTTCTCTTCCAAGTAATGTTGAAATTACTCACATTTCTCTATTTGATAATAGTGTAGAGGGA
ATAATGATGAAGGATTACTCAGTCTTTTCTGTGCAATATCATCCAGAAGAAGCGCCAGGTACGCATGATTCGCATTATTT
GTTTAGACGTTTTATTGACAATATTGTGTTGTATAAAATGAAATCTGCATGA

Upstream 100 bases:

>100_bases
ATTTTTTGCTGCTTCGTGGTTAAATTTGGAATTTTAGAAAGAGTTACGTATTATTTGAAATTCTTGTATACTGTTTTGAA
TCAGTTAAATAGATAATGAA

Downstream 100 bases:

>100_bases
TTTTAATTAGGGTAGGTTTTATTGCTTGATTTTTTAATTTTAGGCGTTTAGTATTAAATACAATAAGAGCTTAAGTTAAG
ATGCTAAAATAGTATTCTCT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MQDAVLILQDGKCFWGKSIGKKGKCIGEVCFTTGMTGYQHTITDPSFADQIITFTFPHIGNVGINHKDNEGKKIFASGVV
MRELSSMSHPSSYISLNDWLEKNNVIGISGVDTRALTRHLREHGSQNGMICSSSKAHALDKLKEYKSVNGIEITNKVSLH
SNFKSDLNAKYRVVIVDFGVKISIVSRLIELGCTVELIRPSTGFAQKVLSMNPDGIVLSNGPGDPQEIGESVVSEIDIII
KSKIPIFGICMGHQLLAITLGAKTIKMDIGHRGSNHPVYDVSSGKVGITSQNHGFVVDSASLPSNVEITHISLFDNSVEG
IMMKDYSVFSVQYHPEEAPGTHDSHYLFRRFIDNIVLYKMKSA

Sequences:

>Translated_363_residues
MQDAVLILQDGKCFWGKSIGKKGKCIGEVCFTTGMTGYQHTITDPSFADQIITFTFPHIGNVGINHKDNEGKKIFASGVV
MRELSSMSHPSSYISLNDWLEKNNVIGISGVDTRALTRHLREHGSQNGMICSSSKAHALDKLKEYKSVNGIEITNKVSLH
SNFKSDLNAKYRVVIVDFGVKISIVSRLIELGCTVELIRPSTGFAQKVLSMNPDGIVLSNGPGDPQEIGESVVSEIDIII
KSKIPIFGICMGHQLLAITLGAKTIKMDIGHRGSNHPVYDVSSGKVGITSQNHGFVVDSASLPSNVEITHISLFDNSVEG
IMMKDYSVFSVQYHPEEAPGTHDSHYLFRRFIDNIVLYKMKSA
>Mature_363_residues
MQDAVLILQDGKCFWGKSIGKKGKCIGEVCFTTGMTGYQHTITDPSFADQIITFTFPHIGNVGINHKDNEGKKIFASGVV
MRELSSMSHPSSYISLNDWLEKNNVIGISGVDTRALTRHLREHGSQNGMICSSSKAHALDKLKEYKSVNGIEITNKVSLH
SNFKSDLNAKYRVVIVDFGVKISIVSRLIELGCTVELIRPSTGFAQKVLSMNPDGIVLSNGPGDPQEIGESVVSEIDIII
KSKIPIFGICMGHQLLAITLGAKTIKMDIGHRGSNHPVYDVSSGKVGITSQNHGFVVDSASLPSNVEITHISLFDNSVEG
IMMKDYSVFSVQYHPEEAPGTHDSHYLFRRFIDNIVLYKMKSA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=365, Percent_Identity=36.4383561643836, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI169790915, Length=368, Percent_Identity=33.695652173913, Blast_Score=188, Evalue=5e-48,
Organism=Homo sapiens, GI21361331, Length=368, Percent_Identity=33.695652173913, Blast_Score=188, Evalue=6e-48,
Organism=Escherichia coli, GI1786215, Length=379, Percent_Identity=43.5356200527704, Blast_Score=330, Evalue=8e-92,
Organism=Escherichia coli, GI1789760, Length=155, Percent_Identity=29.0322580645161, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=366, Percent_Identity=33.879781420765, Blast_Score=209, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6324878, Length=375, Percent_Identity=37.0666666666667, Blast_Score=220, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6322331, Length=393, Percent_Identity=33.587786259542, Blast_Score=212, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6322638, Length=179, Percent_Identity=28.4916201117318, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI45555749, Length=375, Percent_Identity=34.6666666666667, Blast_Score=201, Evalue=6e-52,
Organism=Drosophila melanogaster, GI24642586, Length=375, Percent_Identity=34.6666666666667, Blast_Score=201, Evalue=6e-52,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 39785; Mature: 39785

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDAVLILQDGKCFWGKSIGKKGKCIGEVCFTTGMTGYQHTITDPSFADQIITFTFPHIG
CCCCEEEEECCCEEECCCCCCCCCHHHHHHHHCCCCCCEEECCCCHHHCCEEEEECCCCC
NVGINHKDNEGKKIFASGVVMRELSSMSHPSSYISLNDWLEKNNVIGISGVDTRALTRHL
CCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCEEEHHHHHCCCCEEEECCCCHHHHHHHH
REHGSQNGMICSSSKAHALDKLKEYKSVNGIEITNKVSLHSNFKSDLNAKYRVVIVDFGV
HHHCCCCCEEECCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCEEEEEEEECCC
KISIVSRLIELGCTVELIRPSTGFAQKVLSMNPDGIVLSNGPGDPQEIGESVVSEIDIII
HHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHEEEE
KSKIPIFGICMGHQLLAITLGAKTIKMDIGHRGSNHPVYDVSSGKVGITSQNHGFVVDSA
ECCCCEEEEECCCEEEEEEECCEEEEEECCCCCCCCCEEEECCCEEEEEECCCCEEEECC
SLPSNVEITHISLFDNSVEGIMMKDYSVFSVQYHPEEAPGTHDSHYLFRRFIDNIVLYKM
CCCCCEEEEEEEEECCCCCEEEEECEEEEEEEECCCCCCCCCCHHHHHHHHHHHEEEEEE
KSA
ECC
>Mature Secondary Structure
MQDAVLILQDGKCFWGKSIGKKGKCIGEVCFTTGMTGYQHTITDPSFADQIITFTFPHIG
CCCCEEEEECCCEEECCCCCCCCCHHHHHHHHCCCCCCEEECCCCHHHCCEEEEECCCCC
NVGINHKDNEGKKIFASGVVMRELSSMSHPSSYISLNDWLEKNNVIGISGVDTRALTRHL
CCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCEEEHHHHHCCCCEEEECCCCHHHHHHHH
REHGSQNGMICSSSKAHALDKLKEYKSVNGIEITNKVSLHSNFKSDLNAKYRVVIVDFGV
HHHCCCCCEEECCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCEEEEEEEECCC
KISIVSRLIELGCTVELIRPSTGFAQKVLSMNPDGIVLSNGPGDPQEIGESVVSEIDIII
HHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHEEEE
KSKIPIFGICMGHQLLAITLGAKTIKMDIGHRGSNHPVYDVSSGKVGITSQNHGFVVDSA
ECCCCEEEEECCCEEEEEEECCEEEEEECCCCCCCCCEEEECCCEEEEEECCCCEEEECC
SLPSNVEITHISLFDNSVEGIMMKDYSVFSVQYHPEEAPGTHDSHYLFRRFIDNIVLYKM
CCCCCEEEEEEEEECCCCCEEEEECEEEEEEEECCCCCCCCCCHHHHHHHHHHHEEEEEE
KSA
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]