Click here to switch to the map view.

The map label for this gene is tuf [H]

Identifier: 225630303

GI number: 225630303

Start: 552978

End: 554150

Strand: Reverse

Name: tuf [H]

Synonym: WRi_005210

Alternate gene names: 225630303

Gene position: 554150-552978 (Counterclockwise)

Preceding gene: 225630304

Following gene: 225630302

Centisome position: 38.33

GC content: 42.28

Gene sequence:

>1173_bases
ATGACAGCAGTAGTGGAAGCATTTGGAAAGCCGCATGTAAATGTGGGAACAATAGGACATGTGGATCATGGGAAGACAAC
GTTAACAGCGGCAATAACAAAGCATTATGGAAATTTTGTAGCGTACGATCAGATAGATAAAGCGCCAGAAGAAAGGAAGA
GGGGGATAACGATAGCAACAGCGCATGTTGAGTATCAGACGGAAAAAAGGCATTATGCACACGTTGATTGTCCTGGACAT
GCTGACTATGTAAAGAACATGATAGTAGGTGCAGCACAGATGGATGCGGCGATATTGGTAGTGTCAGGGGTTGATGGGCC
AATGCCACAAACGAGAGAGCATATATTGCTGGCGAAGCAGGTAGGTGTTGGATATATCGTGGTGTATATAAATAAAGCTG
ATGTTGCTGATGCTGATATGATGGATTTAGTAGAAATGGAAGTGAGGGAATTGCTGAATAAATACGGATTTCCAGGGGAT
GAAGTTCCTGTGGTAGTGGGATCTGCATTGAAAGCGCTGGAGGATGACAGCAGCGAATATGGAAAGAAATCAATAGATAA
ATTGATGGAAAAGTTAGATGAATATGTGGCGGTTCCACCAAGGCCTGTGGATTTACCATTTTTATTGCCAATCGAGGATG
TATTTTCGATATCGGGGCGAGGAACAGTGGTAACAGGAAGAATAGAGAAGGGAGAGATAAAGACGGGAGAAGAGATAGAG
ATAATAGGTTTAAAAGCAACACAAAAGACGATATGTACAGGTGTTGAGATGTTTAAGAAGTTGCTGGATAAGGGAAGTGC
AGGGCTCAATGTAGGAATATTGCTGAGAGGAACAAAAAGAGAAGAAGTGGAGAGAGGGCAAGTATTAGCAAAACCGGGGA
CGATAACGCCGCATAGAAAGTTTAAGGCGGAGGTTTATATATTAAAGAAAGAGGAAGGAGGAAGGCATACACCATTTTTT
GCGAATTACCAGCCACAGTTTTATTTAAGGACAACGGATGTAACTGGGAGCATAAAATTACTAGATGGGAAGGAGATGGT
AATGCCAGGAGATAATGTGAGTGTAGAAGTAGAATTGCAAGTACCGATAGCAATGGATAAGGGATTGCGTTTTGCGATAA
GAGAAGGCGGTAGAACTGTTGGTTCTGGTGTTGTTTCTGAAATTTTGGAGTGA

Upstream 100 bases:

>100_bases
GGTTCGATTCCCACTATCCGCTCTTTTTTTTGTTGTATTTTTATAAAATCAACATATTATTTATTGATGTATTTGTATTT
TAGGTAGAAAAGTTTAAATT

Downstream 100 bases:

>100_bases
GTGTAGTAACTAAGATGAAGCAAGATATATATATTAACATTAAGGCTTTCGATTGTTCTTTATTGGAGGAGTGTGTTCGA
AAATTTGTTGATGAATTAAA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MTAVVEAFGKPHVNVGTIGHVDHGKTTLTAAITKHYGNFVAYDQIDKAPEERKRGITIATAHVEYQTEKRHYAHVDCPGH
ADYVKNMIVGAAQMDAAILVVSGVDGPMPQTREHILLAKQVGVGYIVVYINKADVADADMMDLVEMEVRELLNKYGFPGD
EVPVVVGSALKALEDDSSEYGKKSIDKLMEKLDEYVAVPPRPVDLPFLLPIEDVFSISGRGTVVTGRIEKGEIKTGEEIE
IIGLKATQKTICTGVEMFKKLLDKGSAGLNVGILLRGTKREEVERGQVLAKPGTITPHRKFKAEVYILKKEEGGRHTPFF
ANYQPQFYLRTTDVTGSIKLLDGKEMVMPGDNVSVEVELQVPIAMDKGLRFAIREGGRTVGSGVVSEILE

Sequences:

>Translated_390_residues
MTAVVEAFGKPHVNVGTIGHVDHGKTTLTAAITKHYGNFVAYDQIDKAPEERKRGITIATAHVEYQTEKRHYAHVDCPGH
ADYVKNMIVGAAQMDAAILVVSGVDGPMPQTREHILLAKQVGVGYIVVYINKADVADADMMDLVEMEVRELLNKYGFPGD
EVPVVVGSALKALEDDSSEYGKKSIDKLMEKLDEYVAVPPRPVDLPFLLPIEDVFSISGRGTVVTGRIEKGEIKTGEEIE
IIGLKATQKTICTGVEMFKKLLDKGSAGLNVGILLRGTKREEVERGQVLAKPGTITPHRKFKAEVYILKKEEGGRHTPFF
ANYQPQFYLRTTDVTGSIKLLDGKEMVMPGDNVSVEVELQVPIAMDKGLRFAIREGGRTVGSGVVSEILE
>Mature_389_residues
TAVVEAFGKPHVNVGTIGHVDHGKTTLTAAITKHYGNFVAYDQIDKAPEERKRGITIATAHVEYQTEKRHYAHVDCPGHA
DYVKNMIVGAAQMDAAILVVSGVDGPMPQTREHILLAKQVGVGYIVVYINKADVADADMMDLVEMEVRELLNKYGFPGDE
VPVVVGSALKALEDDSSEYGKKSIDKLMEKLDEYVAVPPRPVDLPFLLPIEDVFSISGRGTVVTGRIEKGEIKTGEEIEI
IGLKATQKTICTGVEMFKKLLDKGSAGLNVGILLRGTKREEVERGQVLAKPGTITPHRKFKAEVYILKKEEGGRHTPFFA
NYQPQFYLRTTDVTGSIKLLDGKEMVMPGDNVSVEVELQVPIAMDKGLRFAIREGGRTVGSGVVSEILE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=386, Percent_Identity=56.2176165803109, Blast_Score=454, Evalue=1e-128,
Organism=Homo sapiens, GI223555963, Length=433, Percent_Identity=30.9468822170901, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI5729864, Length=433, Percent_Identity=30.9468822170901, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI4503471, Length=440, Percent_Identity=29.3181818181818, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI4503475, Length=441, Percent_Identity=29.0249433106576, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI194018520, Length=338, Percent_Identity=31.0650887573964, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI194097354, Length=338, Percent_Identity=31.0650887573964, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI194018522, Length=437, Percent_Identity=28.1464530892449, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI46094014, Length=377, Percent_Identity=28.6472148541114, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI54607086, Length=316, Percent_Identity=28.1645569620253, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI4503507, Length=267, Percent_Identity=27.7153558052434, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI53729339, Length=241, Percent_Identity=31.9502074688797, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI53729337, Length=241, Percent_Identity=31.9502074688797, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1790412, Length=386, Percent_Identity=69.9481865284974, Blast_Score=536, Evalue=1e-153,
Organism=Escherichia coli, GI1789737, Length=386, Percent_Identity=69.9481865284974, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI2367247, Length=366, Percent_Identity=29.5081967213115, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI48994988, Length=236, Percent_Identity=31.7796610169492, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1789108, Length=249, Percent_Identity=28.5140562248996, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=394, Percent_Identity=49.492385786802, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI25141371, Length=386, Percent_Identity=43.0051813471503, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17552884, Length=440, Percent_Identity=29.7727272727273, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17569207, Length=440, Percent_Identity=29.7727272727273, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI115532067, Length=435, Percent_Identity=28.2758620689655, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=435, Percent_Identity=28.2758620689655, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32566303, Length=427, Percent_Identity=28.3372365339578, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=436, Percent_Identity=26.1467889908257, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17506081, Length=267, Percent_Identity=28.8389513108614, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI32566301, Length=145, Percent_Identity=37.2413793103448, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17509919, Length=279, Percent_Identity=28.673835125448, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=230, Percent_Identity=27.8260869565217, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=402, Percent_Identity=59.9502487562189, Blast_Score=492, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6325337, Length=438, Percent_Identity=30.1369863013699, Blast_Score=156, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6319594, Length=438, Percent_Identity=30.1369863013699, Blast_Score=156, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6320377, Length=339, Percent_Identity=32.1533923303835, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6322937, Length=466, Percent_Identity=24.2489270386266, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=277, Percent_Identity=28.5198555956679, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6322359, Length=175, Percent_Identity=30.2857142857143, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI281363316, Length=385, Percent_Identity=55.8441558441558, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI17864358, Length=385, Percent_Identity=55.8441558441558, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI19921738, Length=384, Percent_Identity=49.4791666666667, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI45553807, Length=446, Percent_Identity=27.3542600896861, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI45553816, Length=446, Percent_Identity=27.3542600896861, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24651721, Length=446, Percent_Identity=27.3542600896861, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI17864154, Length=446, Percent_Identity=27.3542600896861, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24652838, Length=446, Percent_Identity=27.5784753363229, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI17137572, Length=446, Percent_Identity=27.5784753363229, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI19922690, Length=300, Percent_Identity=30.6666666666667, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17137380, Length=345, Percent_Identity=31.304347826087, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI45550900, Length=432, Percent_Identity=25.9259259259259, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24647046, Length=275, Percent_Identity=27.2727272727273, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24647048, Length=275, Percent_Identity=27.2727272727273, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI78706572, Length=243, Percent_Identity=27.9835390946502, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 42686; Mature: 42555

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAVVEAFGKPHVNVGTIGHVDHGKTTLTAAITKHYGNFVAYDQIDKAPEERKRGITIAT
CCCCHHHCCCCCCCCCEEECCCCCCEEHHHHHHHHCCCEEEEHHHHCCHHHHHCCEEEEE
AHVEYQTEKRHYAHVDCPGHADYVKNMIVGAAQMDAAILVVSGVDGPMPQTREHILLAKQ
EEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHEEEEEE
VGVGYIVVYINKADVADADMMDLVEMEVRELLNKYGFPGDEVPVVVGSALKALEDDSSEY
CCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCCCCHHH
GKKSIDKLMEKLDEYVAVPPRPVDLPFLLPIEDVFSISGRGTVVTGRIEKGEIKTGEEIE
HHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHCCCCCCEEEEEEEECCCCCCCCEEE
IIGLKATQKTICTGVEMFKKLLDKGSAGLNVGILLRGTKREEVERGQVLAKPGTITPHRK
EEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHCCCEEECCCCCCCCCC
FKAEVYILKKEEGGRHTPFFANYQPQFYLRTTDVTGSIKLLDGKEMVMPGDNVSVEVELQ
EEEEEEEEEECCCCCCCCEEECCCCEEEEEEECCCCEEEEECCCEEEECCCCEEEEEEEE
VPIAMDKGLRFAIREGGRTVGSGVVSEILE
EEEEECCCCEEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TAVVEAFGKPHVNVGTIGHVDHGKTTLTAAITKHYGNFVAYDQIDKAPEERKRGITIAT
CCCHHHCCCCCCCCCEEECCCCCCEEHHHHHHHHCCCEEEEHHHHCCHHHHHCCEEEEE
AHVEYQTEKRHYAHVDCPGHADYVKNMIVGAAQMDAAILVVSGVDGPMPQTREHILLAKQ
EEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHEEEEEE
VGVGYIVVYINKADVADADMMDLVEMEVRELLNKYGFPGDEVPVVVGSALKALEDDSSEY
CCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCCCCHHH
GKKSIDKLMEKLDEYVAVPPRPVDLPFLLPIEDVFSISGRGTVVTGRIEKGEIKTGEEIE
HHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHCCCCCCEEEEEEEECCCCCCCCEEE
IIGLKATQKTICTGVEMFKKLLDKGSAGLNVGILLRGTKREEVERGQVLAKPGTITPHRK
EEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHCCCEEECCCCCCCCCC
FKAEVYILKKEEGGRHTPFFANYQPQFYLRTTDVTGSIKLLDGKEMVMPGDNVSVEVELQ
EEEEEEEEEECCCCCCCCEEECCCCEEEEEEECCCCEEEEECCCEEEECCCCEEEEEEEE
VPIAMDKGLRFAIREGGRTVGSGVVSEILE
EEEEECCCCEEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA