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The map label for this gene is dnaG [H]

Identifier: 225630229

GI number: 225630229

Start: 471773

End: 473521

Strand: Direct

Name: dnaG [H]

Synonym: WRi_004380

Alternate gene names: 225630229

Gene position: 471773-473521 (Clockwise)

Preceding gene: 225630226

Following gene: 225630231

Centisome position: 32.63

GC content: 33.79

Gene sequence:

>1749_bases
ATGGATCATATAGATATTATAAAATCAAAATTATTATTATCCGACATAGTAGGTAAAAAAGTCAGATTAATAAAAAGAGG
AGATAGTTTTGTTGGGCTCTGTCCATTTCATAACGAAAAAACCCCATCTTTTTCGGTGAGCAATACTAAGGGGTTATATT
ACTGTTTTGGTTGCTTGGCTAGTGGTGATGCATTTGAATTTATTTCACAAACTGAAGGATTAAGCTTTAAAGAAGCATTG
GAAAAATTAGCATCGGTTACAGGTGTTGAACTGCCCAAAAATCTTAGTATTACAAAAGAGGACAATAAACTATTTTTAGC
GCTTGATTTAGCTGCAAATTGGTTTGCACAAAAAAATCAAGGTGTTGTAGATTATTTAAAGCAGCGTAAGATTTCACCTA
AAATCATAGATCAATTTAAGATAGGTTATGCACCAAGCTCTGGTTTGAAAGAGTATTTAAACTCTTCAGGTATTGAAGAC
AGAATTTTGATTGATATTGGATTAGTAAACAAAAATTTTCGTGATTATTTTTATGACCGGCTGATATTTCCTATACAGAG
CATTGCAGGGAGAGTTATTGGTTTTGGCGGACGTGTGCTAAGTTCTGAGCAACAGCCGAAATATTTAAACAGTCCAGAGA
GTCAGCTCTTTAAGAAGAGGGAGAATTTATACGGATTAAACTTTGCTTTAAGCGAAATACGTAAAAAACAGCACATACTC
GTTGTTGAAGGATATATGGACACTATAGCGCTACATCAAGCGGGAATTAGTAATGCAGTTGCTCCGCTTGGTACCGCAAT
TTCTGCAGAACAGATAAAAAATCTATGGAGATTTGCTAAAGAAATCTCCATCTGTATGGATGGCGATAGTGCTGGACGTC
ATGCTGCTACTCGAGTTGCAGAACTTGCTCTGCCAATATTAGAGCCTGGATACACGTTAAAGTTTGTGACTTTGCCGAGT
AATAAAGATCCATACGATATATGTAACGAACTGGAATACAAGAAAGAAGACGTATTGACTGCGCTTGATCACTCAACAAA
ACTTCATTCTGAATATTTATGGCATCACATAATCGATAGCAATTTGCAAAATTATGAAAAACTTGCTCCGGAAAAATATT
CAATTCTTGAGCACAAGTTCATGGAATATGTAAATACTATTAGCAACAGCAGTATTAGAAGGTATTACAGAGATTATTTT
TACAATAAGGCTAGTGAACTAAGAAGTAGCTTCAAAAAACAGATTTTTAATAGTAAAACTAGAGCAACAAAAGGTGAGTA
TCTTCATAACAAATCTCCAGAGCTAATTGAAGCAGAGCAAAATCAGGCCGTAATTTTGCGTATAGCCGTAGAATTTCCTG
AAATCTTGAACCGTCCTATATTTTTTGAGCAATTTTCTCATTTTGAATTTACTAATGAGATGAAAAGATTACAACAGCGT
ATAATTGATGTAATAAGTAACAAAAACAAACTCGATAAAGAGGCTTTGTTACAAGAATTGAAACAGTTTAACGTTATAAA
GTATGTATTTGAAAAAACTAGTGTACTAAATAGCCAACTGAACGAGAGAAAATCTGCAGAAATTGTGTGGAATAATATAG
TGTTACTAAAAGAATTAAATGCATTACGAAAAGAAAGAACCGAAGCGAGGCTGAGCGGTAATCTTGACTTAGAAGAAAGG
TTAATAGAACAAATAGAGCAAATAGAAAGTAGTATACAAGAAATGCAAATGGAGTTTATTGAAAAGTAA

Upstream 100 bases:

>100_bases
CATTGTCGGTAAATCTAAATTGCTTTAGTTATAGAAATAAATTTCGAGTCAGATGTGTATAAATTCTATATAATTTTTAT
CCTACTACTAATTTAACAAG

Downstream 100 bases:

>100_bases
ATATCGTCTCGTTAGTCAACAAGAAAATTACAAAATAAGACAATATTTTACATAAGATTGAAATTTTTAACTAATTAAGC
CTTCCTCCAACAGCTTTGCC

Product: DNA primase, DnaG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MDHIDIIKSKLLLSDIVGKKVRLIKRGDSFVGLCPFHNEKTPSFSVSNTKGLYYCFGCLASGDAFEFISQTEGLSFKEAL
EKLASVTGVELPKNLSITKEDNKLFLALDLAANWFAQKNQGVVDYLKQRKISPKIIDQFKIGYAPSSGLKEYLNSSGIED
RILIDIGLVNKNFRDYFYDRLIFPIQSIAGRVIGFGGRVLSSEQQPKYLNSPESQLFKKRENLYGLNFALSEIRKKQHIL
VVEGYMDTIALHQAGISNAVAPLGTAISAEQIKNLWRFAKEISICMDGDSAGRHAATRVAELALPILEPGYTLKFVTLPS
NKDPYDICNELEYKKEDVLTALDHSTKLHSEYLWHHIIDSNLQNYEKLAPEKYSILEHKFMEYVNTISNSSIRRYYRDYF
YNKASELRSSFKKQIFNSKTRATKGEYLHNKSPELIEAEQNQAVILRIAVEFPEILNRPIFFEQFSHFEFTNEMKRLQQR
IIDVISNKNKLDKEALLQELKQFNVIKYVFEKTSVLNSQLNERKSAEIVWNNIVLLKELNALRKERTEARLSGNLDLEER
LIEQIEQIESSIQEMQMEFIEK

Sequences:

>Translated_582_residues
MDHIDIIKSKLLLSDIVGKKVRLIKRGDSFVGLCPFHNEKTPSFSVSNTKGLYYCFGCLASGDAFEFISQTEGLSFKEAL
EKLASVTGVELPKNLSITKEDNKLFLALDLAANWFAQKNQGVVDYLKQRKISPKIIDQFKIGYAPSSGLKEYLNSSGIED
RILIDIGLVNKNFRDYFYDRLIFPIQSIAGRVIGFGGRVLSSEQQPKYLNSPESQLFKKRENLYGLNFALSEIRKKQHIL
VVEGYMDTIALHQAGISNAVAPLGTAISAEQIKNLWRFAKEISICMDGDSAGRHAATRVAELALPILEPGYTLKFVTLPS
NKDPYDICNELEYKKEDVLTALDHSTKLHSEYLWHHIIDSNLQNYEKLAPEKYSILEHKFMEYVNTISNSSIRRYYRDYF
YNKASELRSSFKKQIFNSKTRATKGEYLHNKSPELIEAEQNQAVILRIAVEFPEILNRPIFFEQFSHFEFTNEMKRLQQR
IIDVISNKNKLDKEALLQELKQFNVIKYVFEKTSVLNSQLNERKSAEIVWNNIVLLKELNALRKERTEARLSGNLDLEER
LIEQIEQIESSIQEMQMEFIEK
>Mature_582_residues
MDHIDIIKSKLLLSDIVGKKVRLIKRGDSFVGLCPFHNEKTPSFSVSNTKGLYYCFGCLASGDAFEFISQTEGLSFKEAL
EKLASVTGVELPKNLSITKEDNKLFLALDLAANWFAQKNQGVVDYLKQRKISPKIIDQFKIGYAPSSGLKEYLNSSGIED
RILIDIGLVNKNFRDYFYDRLIFPIQSIAGRVIGFGGRVLSSEQQPKYLNSPESQLFKKRENLYGLNFALSEIRKKQHIL
VVEGYMDTIALHQAGISNAVAPLGTAISAEQIKNLWRFAKEISICMDGDSAGRHAATRVAELALPILEPGYTLKFVTLPS
NKDPYDICNELEYKKEDVLTALDHSTKLHSEYLWHHIIDSNLQNYEKLAPEKYSILEHKFMEYVNTISNSSIRRYYRDYF
YNKASELRSSFKKQIFNSKTRATKGEYLHNKSPELIEAEQNQAVILRIAVEFPEILNRPIFFEQFSHFEFTNEMKRLQQR
IIDVISNKNKLDKEALLQELKQFNVIKYVFEKTSVLNSQLNERKSAEIVWNNIVLLKELNALRKERTEARLSGNLDLEER
LIEQIEQIESSIQEMQMEFIEK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=425, Percent_Identity=36.2352941176471, Blast_Score=242, Evalue=6e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 66997; Mature: 66997

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHIDIIKSKLLLSDIVGKKVRLIKRGDSFVGLCPFHNEKTPSFSVSNTKGLYYCFGCLA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHC
SGDAFEFISQTEGLSFKEALEKLASVTGVELPKNLSITKEDNKLFLALDLAANWFAQKNQ
CCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCEEEEEEEHHHHHHHHCCC
GVVDYLKQRKISPKIIDQFKIGYAPSSGLKEYLNSSGIEDRILIDIGLVNKNFRDYFYDR
CHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHH
LIFPIQSIAGRVIGFGGRVLSSEQQPKYLNSPESQLFKKRENLYGLNFALSEIRKKQHIL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCEE
VVEGYMDTIALHQAGISNAVAPLGTAISAEQIKNLWRFAKEISICMDGDSAGRHAATRVA
EEECHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHH
ELALPILEPGYTLKFVTLPSNKDPYDICNELEYKKEDVLTALDHSTKLHSEYLWHHIIDS
HHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLQNYEKLAPEKYSILEHKFMEYVNTISNSSIRRYYRDYFYNKASELRSSFKKQIFNSKT
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHH
RATKGEYLHNKSPELIEAEQNQAVILRIAVEFPEILNRPIFFEQFSHFEFTNEMKRLQQR
CCCCCCHHCCCCCCCEECCCCCEEEEEEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IIDVISNKNKLDKEALLQELKQFNVIKYVFEKTSVLNSQLNERKSAEIVWNNIVLLKELN
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
ALRKERTEARLSGNLDLEERLIEQIEQIESSIQEMQMEFIEK
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDHIDIIKSKLLLSDIVGKKVRLIKRGDSFVGLCPFHNEKTPSFSVSNTKGLYYCFGCLA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHC
SGDAFEFISQTEGLSFKEALEKLASVTGVELPKNLSITKEDNKLFLALDLAANWFAQKNQ
CCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCEEEEEEEHHHHHHHHCCC
GVVDYLKQRKISPKIIDQFKIGYAPSSGLKEYLNSSGIEDRILIDIGLVNKNFRDYFYDR
CHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHH
LIFPIQSIAGRVIGFGGRVLSSEQQPKYLNSPESQLFKKRENLYGLNFALSEIRKKQHIL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCEE
VVEGYMDTIALHQAGISNAVAPLGTAISAEQIKNLWRFAKEISICMDGDSAGRHAATRVA
EEECHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHH
ELALPILEPGYTLKFVTLPSNKDPYDICNELEYKKEDVLTALDHSTKLHSEYLWHHIIDS
HHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLQNYEKLAPEKYSILEHKFMEYVNTISNSSIRRYYRDYFYNKASELRSSFKKQIFNSKT
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHH
RATKGEYLHNKSPELIEAEQNQAVILRIAVEFPEILNRPIFFEQFSHFEFTNEMKRLQQR
CCCCCCHHCCCCCCCEECCCCCEEEEEEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IIDVISNKNKLDKEALLQELKQFNVIKYVFEKTSVLNSQLNERKSAEIVWNNIVLLKELN
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
ALRKERTEARLSGNLDLEERLIEQIEQIESSIQEMQMEFIEK
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]