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The map label for this gene is dnaB [H]

Identifier: 225630226

GI number: 225630226

Start: 464431

End: 465882

Strand: Direct

Name: dnaB [H]

Synonym: WRi_004330

Alternate gene names: 225630226

Gene position: 464431-465882 (Clockwise)

Preceding gene: 225630221

Following gene: 225630229

Centisome position: 32.12

GC content: 36.91

Gene sequence:

>1452_bases
ATGAATAAATTAGCTGATCTCCTTGATTCAAGAGGTGTAGACAAAGAAATATGTAAATTACCGCATAATTTAGAAGCTGA
ACAGATGCTTATTGGTGCAATGATTCGTGATAATAGAATCTGTGATGCGGTTGAAGACACAATTACAGCAGAAAATTTCT
ATGATCCACTGCATCAAAGTATTTTTACGCAAATATCTAAAACCAGAAAACATGGCATAGTTGCCAATGAACTGAGCCTC
AAAATGTTTTTTGAGAACGACCAAGCGTTTACCGAATGTGGTGGGGTAGAGTACTTAGCAAAGCTTGCAGCAAAGGCAAG
TATTGCACTTGATATTTATAGTTTGACTAGAATAATCCGTGATACTTACTTAAGGAGATGTTTAATCAAGCTCGGGCAAG
AAATAGTTGGTGATAGCTACAACTACGATATTGAAAATCCTGCACAGGCGCAAATTGAACAAGCAATGACAAAATTGTTC
AATTTGGCAGTAAAAAAACAAGGTGAGAAAACATATATAAAACTTGCAAGTTCAATTAAAGATGTAGTTGAGAAGATCAG
CACACTGAAAAATAATCCAGAAGCGCTAGGCGTTACAACCGGACTTCAAGATCTAAATCAGCTTCTTGGTGGTCTACAAA
AATCTGATTTATTAATTTTAGCTGCAAGGCCTTCTATGGGTAAAACAGCATTGGCGCTAAACATCGCACTTAATGCTTGT
AAGATGTTGCAAAAAAGAGCAGATAAGCAACACTATGTAGCGTTTTTCTCGCTTGAAATGTCAGCAGAGCAATTAACTGC
AAGGCTGATCACTATAGATTCAGGGATTAGTTATTATAAGGCATTAACCGGGAGAATTAGTGATTTTGAATTGCATGAAT
TTATCAATACTAGTACAGAATTATCTGAACTGCCTTTCATCATAGATGACACCCCTGCGCTATCAATTAGTGCCCTTCGC
ACCAGAATACGCTTGCTGTATCAATTATATAATGTAGAAGTGGTATTCATTGATTATTTGCAGCTAATCAGAGGAACAAC
AAAAAGGAGTAATGAAAATAGAGTGCAAGAAATCTCGGAAGTGACGCAGGGTTTGAAAGCAATTGCAAAGGAGCTAAATA
TTCCCATTGTTGCACTATCTCAGCTCTCTCGTTCTGTTGAGCAGAGGGATGATAAAAAACCACAACTTTCTGATTTACGT
GATTCAGGGAGCATAGAGCAAGACGCAGATATAGTAATGTTTCTCTATAGAGAAGAGTATTATGAACTAAGAAAACAGCC
AAATGAAGGAAACAATAAACATCGGGAATGGCAAGAGAAAATGGAAAAAATTAGAAACATTGCAGAGCTCATTATTGCAA
AGCAGAGAAATGGACCAATTGGCAGTGTGAAGCTATATTTTGACTCTAACAGAGGTGCATTCAAAGATTACACGGAAAGG
CATAGTTCATAG

Upstream 100 bases:

>100_bases
TTTTAATGCTATAGGCAACACGGAATTAGTCAAATTTTATATGTCTTTCAAATCAAAACTAAATATTCTACAATAATCGA
TAGTTACTCTTAAATGCAGA

Downstream 100 bases:

>100_bases
CTTGGCAGCAATCTTCTTGTTCCCAAGTTCATTGATTATTTTTTTTATGTAAAGATAGCTTGACAACTTCTTAAAAATCC
TTTTATAATGGTATTATATT

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MNKLADLLDSRGVDKEICKLPHNLEAEQMLIGAMIRDNRICDAVEDTITAENFYDPLHQSIFTQISKTRKHGIVANELSL
KMFFENDQAFTECGGVEYLAKLAAKASIALDIYSLTRIIRDTYLRRCLIKLGQEIVGDSYNYDIENPAQAQIEQAMTKLF
NLAVKKQGEKTYIKLASSIKDVVEKISTLKNNPEALGVTTGLQDLNQLLGGLQKSDLLILAARPSMGKTALALNIALNAC
KMLQKRADKQHYVAFFSLEMSAEQLTARLITIDSGISYYKALTGRISDFELHEFINTSTELSELPFIIDDTPALSISALR
TRIRLLYQLYNVEVVFIDYLQLIRGTTKRSNENRVQEISEVTQGLKAIAKELNIPIVALSQLSRSVEQRDDKKPQLSDLR
DSGSIEQDADIVMFLYREEYYELRKQPNEGNNKHREWQEKMEKIRNIAELIIAKQRNGPIGSVKLYFDSNRGAFKDYTER
HSS

Sequences:

>Translated_483_residues
MNKLADLLDSRGVDKEICKLPHNLEAEQMLIGAMIRDNRICDAVEDTITAENFYDPLHQSIFTQISKTRKHGIVANELSL
KMFFENDQAFTECGGVEYLAKLAAKASIALDIYSLTRIIRDTYLRRCLIKLGQEIVGDSYNYDIENPAQAQIEQAMTKLF
NLAVKKQGEKTYIKLASSIKDVVEKISTLKNNPEALGVTTGLQDLNQLLGGLQKSDLLILAARPSMGKTALALNIALNAC
KMLQKRADKQHYVAFFSLEMSAEQLTARLITIDSGISYYKALTGRISDFELHEFINTSTELSELPFIIDDTPALSISALR
TRIRLLYQLYNVEVVFIDYLQLIRGTTKRSNENRVQEISEVTQGLKAIAKELNIPIVALSQLSRSVEQRDDKKPQLSDLR
DSGSIEQDADIVMFLYREEYYELRKQPNEGNNKHREWQEKMEKIRNIAELIIAKQRNGPIGSVKLYFDSNRGAFKDYTER
HSS
>Mature_483_residues
MNKLADLLDSRGVDKEICKLPHNLEAEQMLIGAMIRDNRICDAVEDTITAENFYDPLHQSIFTQISKTRKHGIVANELSL
KMFFENDQAFTECGGVEYLAKLAAKASIALDIYSLTRIIRDTYLRRCLIKLGQEIVGDSYNYDIENPAQAQIEQAMTKLF
NLAVKKQGEKTYIKLASSIKDVVEKISTLKNNPEALGVTTGLQDLNQLLGGLQKSDLLILAARPSMGKTALALNIALNAC
KMLQKRADKQHYVAFFSLEMSAEQLTARLITIDSGISYYKALTGRISDFELHEFINTSTELSELPFIIDDTPALSISALR
TRIRLLYQLYNVEVVFIDYLQLIRGTTKRSNENRVQEISEVTQGLKAIAKELNIPIVALSQLSRSVEQRDDKKPQLSDLR
DSGSIEQDADIVMFLYREEYYELRKQPNEGNNKHREWQEKMEKIRNIAELIIAKQRNGPIGSVKLYFDSNRGAFKDYTER
HSS

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=481, Percent_Identity=36.5904365904366, Blast_Score=278, Evalue=4e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 54820; Mature: 54820

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLADLLDSRGVDKEICKLPHNLEAEQMLIGAMIRDNRICDAVEDTITAENFYDPLHQS
CCHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
IFTQISKTRKHGIVANELSLKMFFENDQAFTECGGVEYLAKLAAKASIALDIYSLTRIIR
HHHHHHHHHHCCCEEECEEEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
DTYLRRCLIKLGQEIVGDSYNYDIENPAQAQIEQAMTKLFNLAVKKQGEKTYIKLASSIK
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
DVVEKISTLKNNPEALGVTTGLQDLNQLLGGLQKSDLLILAARPSMGKTALALNIALNAC
HHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHH
KMLQKRADKQHYVAFFSLEMSAEQLTARLITIDSGISYYKALTGRISDFELHEFINTSTE
HHHHHHCCCCCEEEEEEEEHHHHHHHHHHEEECCCHHHHHHHHCCCCCHHHHHHHCCCCH
LSELPFIIDDTPALSISALRTRIRLLYQLYNVEVVFIDYLQLIRGTTKRSNENRVQEISE
HHHCCEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHH
VTQGLKAIAKELNIPIVALSQLSRSVEQRDDKKPQLSDLRDSGSIEQDADIVMFLYREEY
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHH
YELRKQPNEGNNKHREWQEKMEKIRNIAELIIAKQRNGPIGSVKLYFDSNRGAFKDYTER
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHH
HSS
CCC
>Mature Secondary Structure
MNKLADLLDSRGVDKEICKLPHNLEAEQMLIGAMIRDNRICDAVEDTITAENFYDPLHQS
CCHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
IFTQISKTRKHGIVANELSLKMFFENDQAFTECGGVEYLAKLAAKASIALDIYSLTRIIR
HHHHHHHHHHCCCEEECEEEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
DTYLRRCLIKLGQEIVGDSYNYDIENPAQAQIEQAMTKLFNLAVKKQGEKTYIKLASSIK
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
DVVEKISTLKNNPEALGVTTGLQDLNQLLGGLQKSDLLILAARPSMGKTALALNIALNAC
HHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHH
KMLQKRADKQHYVAFFSLEMSAEQLTARLITIDSGISYYKALTGRISDFELHEFINTSTE
HHHHHHCCCCCEEEEEEEEHHHHHHHHHHEEECCCHHHHHHHHCCCCCHHHHHHHCCCCH
LSELPFIIDDTPALSISALRTRIRLLYQLYNVEVVFIDYLQLIRGTTKRSNENRVQEISE
HHHCCEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHH
VTQGLKAIAKELNIPIVALSQLSRSVEQRDDKKPQLSDLRDSGSIEQDADIVMFLYREEY
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHH
YELRKQPNEGNNKHREWQEKMEKIRNIAELIIAKQRNGPIGSVKLYFDSNRGAFKDYTER
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHH
HSS
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA