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The map label for this gene is ispE [H]

Identifier: 225630220

GI number: 225630220

Start: 457221

End: 458087

Strand: Reverse

Name: ispE [H]

Synonym: WRi_004260

Alternate gene names: 225630220

Gene position: 458087-457221 (Counterclockwise)

Preceding gene: 225630222

Following gene: 225630219

Centisome position: 31.68

GC content: 37.49

Gene sequence:

>867_bases
ATGAAAAGTTTTTGTGTAAAGGCGCCTGCAAAAATCAATCTTTTTTTGCATGTTGTAGACAAAAAAGAAACAGGGTATCA
CTTGATTGAAGGTTTATTTGTCTTTGCTAATCTCTCTAATTTTTTGGAAATAAAAGTAGGTGAGAAGGATTCTAGATACG
ATAACTCTACAGTTGAATTTATAAACTCCGAGTCTAAAATAAATAACCAATATAACACTGTAATGAAAGCGGTTAATCTG
CTGCTTAGGCATGCTCCTGTACGCACTAAAGTCACTGTAAAGGTTGTAAAAAACATACCAATTGCTGCAGGACTTGGTAG
CGGATCCTCAGATGCTGGAGCTGTGGTACGTACGCTAGGGAAGCTGTGGGAGATTGATAGGACGATTTTAAATGAAATAG
CCTTAAACGTTGGTGCTGATGTTCCCGCAAGCGTAGATAGCAAGCCAGTTTTTGTTAGAGGTATTGGCGAAGAATTATGC
CACATTAAAAAATTCTCTTTACCTACAAATGTGGTGCTTGTGAAACCGAAAAAAAGGTTTTTGAGTACACCAGAGGTATT
TTCCAAACATGAAGGAAAATTTTCTGAGCCAATTAAATGGAGCGATGACGCTGAAAAGGATTTGTTGAAGCTTCTTAAAG
AGACGAGAAACGATCTTCAAGAAATAGCAATAAGCTTTGTACCTGAAATTAAGGATGTGATATCAACACTTGAGTCACAA
GAAGGCTCTATACTTTCTCGCATGTCAGGCAGTGGTGTATCATGTTTTGGAATATTCGATAGTGAGGAAAATGCAAAAGC
TGCTGCAGTTAATATCGGAAAAAAGCAACCAGAATGGTGGGTATGTGACACTCAATTAATAGTTTGA

Upstream 100 bases:

>100_bases
TTATGGCATTTGATCTCATCAGAAGTGCGAGAACCTTATAAAAGTTGACTAGAATAGCATTATACATATAATGTAAAAAT
AGTGTTAGTGAAGATATATA

Downstream 100 bases:

>100_bases
CTTATGGATGTAACTCCCTTAATTTCCGAGAATAAAAATCTTATAAATGGTTACAGGGAAGGAAAATTTTTGGTGAATAA
TAGAGAATATTGCGGCTCAA

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKSFCVKAPAKINLFLHVVDKKETGYHLIEGLFVFANLSNFLEIKVGEKDSRYDNSTVEFINSESKINNQYNTVMKAVNL
LLRHAPVRTKVTVKVVKNIPIAAGLGSGSSDAGAVVRTLGKLWEIDRTILNEIALNVGADVPASVDSKPVFVRGIGEELC
HIKKFSLPTNVVLVKPKKRFLSTPEVFSKHEGKFSEPIKWSDDAEKDLLKLLKETRNDLQEIAISFVPEIKDVISTLESQ
EGSILSRMSGSGVSCFGIFDSEENAKAAAVNIGKKQPEWWVCDTQLIV

Sequences:

>Translated_288_residues
MKSFCVKAPAKINLFLHVVDKKETGYHLIEGLFVFANLSNFLEIKVGEKDSRYDNSTVEFINSESKINNQYNTVMKAVNL
LLRHAPVRTKVTVKVVKNIPIAAGLGSGSSDAGAVVRTLGKLWEIDRTILNEIALNVGADVPASVDSKPVFVRGIGEELC
HIKKFSLPTNVVLVKPKKRFLSTPEVFSKHEGKFSEPIKWSDDAEKDLLKLLKETRNDLQEIAISFVPEIKDVISTLESQ
EGSILSRMSGSGVSCFGIFDSEENAKAAAVNIGKKQPEWWVCDTQLIV
>Mature_288_residues
MKSFCVKAPAKINLFLHVVDKKETGYHLIEGLFVFANLSNFLEIKVGEKDSRYDNSTVEFINSESKINNQYNTVMKAVNL
LLRHAPVRTKVTVKVVKNIPIAAGLGSGSSDAGAVVRTLGKLWEIDRTILNEIALNVGADVPASVDSKPVFVRGIGEELC
HIKKFSLPTNVVLVKPKKRFLSTPEVFSKHEGKFSEPIKWSDDAEKDLLKLLKETRNDLQEIAISFVPEIKDVISTLESQ
EGSILSRMSGSGVSCFGIFDSEENAKAAAVNIGKKQPEWWVCDTQLIV

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [H]

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787459, Length=281, Percent_Identity=28.4697508896797, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N [H]

EC number: =2.7.1.148 [H]

Molecular weight: Translated: 31846; Mature: 31846

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSFCVKAPAKINLFLHVVDKKETGYHLIEGLFVFANLSNFLEIKVGEKDSRYDNSTVEF
CCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHH
INSESKINNQYNTVMKAVNLLLRHAPVRTKVTVKVVKNIPIAAGLGSGSSDAGAVVRTLG
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCCCCHHHHHHHHH
KLWEIDRTILNEIALNVGADVPASVDSKPVFVRGIGEELCHIKKFSLPTNVVLVKPKKRF
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCCEEEECCHHHH
LSTPEVFSKHEGKFSEPIKWSDDAEKDLLKLLKETRNDLQEIAISFVPEIKDVISTLESQ
CCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EGSILSRMSGSGVSCFGIFDSEENAKAAAVNIGKKQPEWWVCDTQLIV
CCHHHHHCCCCCEEEEEEECCCCCCCEEEEECCCCCCCEEEECEEEEC
>Mature Secondary Structure
MKSFCVKAPAKINLFLHVVDKKETGYHLIEGLFVFANLSNFLEIKVGEKDSRYDNSTVEF
CCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHH
INSESKINNQYNTVMKAVNLLLRHAPVRTKVTVKVVKNIPIAAGLGSGSSDAGAVVRTLG
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCCCCHHHHHHHHH
KLWEIDRTILNEIALNVGADVPASVDSKPVFVRGIGEELCHIKKFSLPTNVVLVKPKKRF
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCCEEEECCHHHH
LSTPEVFSKHEGKFSEPIKWSDDAEKDLLKLLKETRNDLQEIAISFVPEIKDVISTLESQ
CCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EGSILSRMSGSGVSCFGIFDSEENAKAAAVNIGKKQPEWWVCDTQLIV
CCHHHHHCCCCCEEEEEEECCCCCCCEEEEECCCCCCCEEEECEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA