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The map label for this gene is prfB [H]

Identifier: 225630212

GI number: 225630212

Start: 449287

End: 450297

Strand: Direct

Name: prfB [H]

Synonym: WRi_004150

Alternate gene names: 225630212

Gene position: 449287-450297 (Clockwise)

Preceding gene: 225630210

Following gene: 225630221

Centisome position: 31.07

GC content: 37.59

Gene sequence:

>1011_bases
TTGCGTCTTGAAGAGCTGGATTCCCAGGCGTCGAATGATAATCTGTGGCAAGACAATCAAAAGGCACAAGAAATTTTAAA
AGAACGTTCTAAAATTAAGAATGACGTAGAGTCGTTTTTAAAGCTGGAAAGCGATTACAACGATGCCATCAGCTTAATGA
AGTCTGCTATTGATGAAAATGATGAAGAATTTTTCTCTGAAGTTGAAAATGAATTAGCTAAGCTAGAGAAATTAATCAAG
TATAAAGAAACAGAGTCCTTATTTACTGGTGAAGCAGATAATAATAACTGCTTTTTAGAAATCCACTCAGGAGCTGGTGG
AACAGAGAGCAATGATTGGGCTGAAATGCTGATGCGCATGTATGTAAGGTGGGCAGAAATTTATCACAACTTTAAAGTTG
AAGTTGTAGAAAAATTAGAAGGAGAGTCGGTTGGTATAAAGTCCGCAATGATAAAAATCGTTGGAGAAAAAGCTTATGGG
TGGGCAAAAAGTGAAAGTGGAATTCACAGGCTGGTTAGAATATCGCCGTTTGATGCAAATGGTAAACGTCATACCAGTTT
TGCAAGTGTAGGAGTAACTCCAGTGATCGAAGATTCAATTGATATTGCTGTGGATGAAAAGGATCTAAAGATCGACACCT
ACCGTGCTTCCGGAGCAGGCGGTCAGCATGTGAACAAAACTGAAAGTGCAGTACGCATTACGCATATTCCAACTGGTGTT
GTAGTTCAATGCCAAAATGGTCGTTCTCAACACAGAAATAAAGATGAGGCATTGAAATTACTTAAAGGACGTTTATACCA
AATTGAACTGGAAAAAAAAGAACAGAAAATGGCTGAAGAATATGGTAAAAAATGCGATATAGGCTGGGGCAATCAGATCA
GATCGTACGTTATGCATCCATATCAAATGGTGAAGGATTTAAGAACCGGACATGAAGTGGGCAATATAAATTCTGTTTTT
GATGGTAATATAGATTGTTTCATAGTTAGTGTGCTTACTAAGCAAAATTAG

Upstream 100 bases:

>100_bases
AGTATAATGAAAACTTATCCAGAAATTCTTGAACACTTTCAAAGCTTAAATAAAAGTATTTCTCTTATCAGGAGGTGTCT
TTGACATAGAAAAATTAAAG

Downstream 100 bases:

>100_bases
GTAGGGTTGACTTTCTTGCCATAATATATGATAATTAAAATATTGTTAAAGTAGGAGTAAAGTTATGGCGATTAAATATG
ATCAGTGGAAAAGTGACCTT

Product: Protein chain release factor B

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MRLEELDSQASNDNLWQDNQKAQEILKERSKIKNDVESFLKLESDYNDAISLMKSAIDENDEEFFSEVENELAKLEKLIK
YKETESLFTGEADNNNCFLEIHSGAGGTESNDWAEMLMRMYVRWAEIYHNFKVEVVEKLEGESVGIKSAMIKIVGEKAYG
WAKSESGIHRLVRISPFDANGKRHTSFASVGVTPVIEDSIDIAVDEKDLKIDTYRASGAGGQHVNKTESAVRITHIPTGV
VVQCQNGRSQHRNKDEALKLLKGRLYQIELEKKEQKMAEEYGKKCDIGWGNQIRSYVMHPYQMVKDLRTGHEVGNINSVF
DGNIDCFIVSVLTKQN

Sequences:

>Translated_336_residues
MRLEELDSQASNDNLWQDNQKAQEILKERSKIKNDVESFLKLESDYNDAISLMKSAIDENDEEFFSEVENELAKLEKLIK
YKETESLFTGEADNNNCFLEIHSGAGGTESNDWAEMLMRMYVRWAEIYHNFKVEVVEKLEGESVGIKSAMIKIVGEKAYG
WAKSESGIHRLVRISPFDANGKRHTSFASVGVTPVIEDSIDIAVDEKDLKIDTYRASGAGGQHVNKTESAVRITHIPTGV
VVQCQNGRSQHRNKDEALKLLKGRLYQIELEKKEQKMAEEYGKKCDIGWGNQIRSYVMHPYQMVKDLRTGHEVGNINSVF
DGNIDCFIVSVLTKQN
>Mature_336_residues
MRLEELDSQASNDNLWQDNQKAQEILKERSKIKNDVESFLKLESDYNDAISLMKSAIDENDEEFFSEVENELAKLEKLIK
YKETESLFTGEADNNNCFLEIHSGAGGTESNDWAEMLMRMYVRWAEIYHNFKVEVVEKLEGESVGIKSAMIKIVGEKAYG
WAKSESGIHRLVRISPFDANGKRHTSFASVGVTPVIEDSIDIAVDEKDLKIDTYRASGAGGQHVNKTESAVRITHIPTGV
VVQCQNGRSQHRNKDEALKLLKGRLYQIELEKKEQKMAEEYGKKCDIGWGNQIRSYVMHPYQMVKDLRTGHEVGNINSVF
DGNIDCFIVSVLTKQN

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=329, Percent_Identity=31.9148936170213, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI34577120, Length=249, Percent_Identity=38.5542168674699, Blast_Score=148, Evalue=6e-36,
Organism=Homo sapiens, GI166795305, Length=241, Percent_Identity=31.9502074688797, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI2367172, Length=331, Percent_Identity=50.4531722054381, Blast_Score=353, Evalue=6e-99,
Organism=Escherichia coli, GI1787462, Length=243, Percent_Identity=41.1522633744856, Blast_Score=188, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=292, Percent_Identity=30.4794520547945, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=311, Percent_Identity=32.475884244373, Blast_Score=142, Evalue=7e-35,
Organism=Drosophila melanogaster, GI19921226, Length=229, Percent_Identity=39.3013100436681, Blast_Score=149, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 38148; Mature: 38148

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLEELDSQASNDNLWQDNQKAQEILKERSKIKNDVESFLKLESDYNDAISLMKSAIDEN
CCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
DEEFFSEVENELAKLEKLIKYKETESLFTGEADNNNCFLEIHSGAGGTESNDWAEMLMRM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHH
YVRWAEIYHNFKVEVVEKLEGESVGIKSAMIKIVGEKAYGWAKSESGIHRLVRISPFDAN
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHEEEECCCCCC
GKRHTSFASVGVTPVIEDSIDIAVDEKDLKIDTYRASGAGGQHVNKTESAVRITHIPTGV
CCCCCCHHHCCCCEEECCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCE
VVQCQNGRSQHRNKDEALKLLKGRLYQIELEKKEQKMAEEYGKKCDIGWGNQIRSYVMHP
EEEECCCCCHHCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHCH
YQMVKDLRTGHEVGNINSVFDGNIDCFIVSVLTKQN
HHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEHHCCC
>Mature Secondary Structure
MRLEELDSQASNDNLWQDNQKAQEILKERSKIKNDVESFLKLESDYNDAISLMKSAIDEN
CCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
DEEFFSEVENELAKLEKLIKYKETESLFTGEADNNNCFLEIHSGAGGTESNDWAEMLMRM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHH
YVRWAEIYHNFKVEVVEKLEGESVGIKSAMIKIVGEKAYGWAKSESGIHRLVRISPFDAN
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHEEEECCCCCC
GKRHTSFASVGVTPVIEDSIDIAVDEKDLKIDTYRASGAGGQHVNKTESAVRITHIPTGV
CCCCCCHHHCCCCEEECCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCE
VVQCQNGRSQHRNKDEALKLLKGRLYQIELEKKEQKMAEEYGKKCDIGWGNQIRSYVMHP
EEEECCCCCHHCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHCH
YQMVKDLRTGHEVGNINSVFDGNIDCFIVSVLTKQN
HHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA