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The map label for this gene is lipA [H]

Identifier: 225630196

GI number: 225630196

Start: 426208

End: 427071

Strand: Reverse

Name: lipA [H]

Synonym: WRi_003970

Alternate gene names: 225630196

Gene position: 427071-426208 (Counterclockwise)

Preceding gene: 225630197

Following gene: 225630195

Centisome position: 29.54

GC content: 38.31

Gene sequence:

>864_bases
ATGCATAGCAAACCTCAATGGCTCAGAGCAAAAGCTCCGACCGGTGAAGTATTCAATGAAACCTTAAACATCGTTAAACT
GCATAACTTGCATACAGTGTGCGAAGAGGCTGCATGTCCAAACATTGGTGAATGTTGGAATAAACGTCATGCTACTGTGA
TGATTCTCGGTTCTGTTTGCACTCGTGCTTGTGCATTTTGCAACGTTGCAACTGGCATTCCTGATAAACTAGACCCTCAT
GAACCAGAAAATTTAGCAAAAGCAATAAAAAAGTTAAACTTAAAACATGTTGTCATTACTTCTGTTGATCGTGATGATTT
ACCAGATGGTGGCGCAAATCAGTTTATACAGTGCATAGAAGAAATTAGAAAGATAACTTCAGAAACAACAATAGAGATTT
TAACTCCTGATTTTTTAAATAAGAAAGGAGCATTTGAAGCAATTGCTGTTGCATCACCTGATGTCTATAACCACAATATT
GAAACAGTGCCGAGATTGTATGCAAAAATAAGACCACGAGCTCGCTATTTTCATTCACTATATTTACTGAAGATGGTAAA
GCAGATTAATCCCAAAGTTTTCACAAAGTCAGGGCTTATGGTCGGTCTTGGAGAGACAAAAGAGGAAATATTTCAGGTTA
TGGACGATTTGCGCAGTGCTGAGGTTGATTTTATTACAATTGGTCAATATCTACAACCAACTCCAAAACATGCAAAACTT
GATAGGTATGTTACCCCAGAAGAGTTTGAGCATTATAAATACATTGCTTACTCCAAAGGTTTCTTAGTGGTTGCATCAAG
CCCACTAACTCGGTCATCATACCACGCTGAAGAAGATTTTAACAGGCTCAAGGCCTGTCGTTGA

Upstream 100 bases:

>100_bases
CTCAACACAAGAACGATTAAACTAAGTGAGAAAGCGCAGAAGATAAAAAGAAGGTTGAAAAAAGTTTTAGTAAAGCAAGA
GGTAGAGTTAGCAGTTTCAG

Downstream 100 bases:

>100_bases
CATTTGTGTTAATTCAAATTAATTTTTACTAATTATAAAGGAGTTTTTACGTAATGTCTACTATAAAAATCGAGAAGAAA
TCACAATTAGTTAAAGAGGT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MHSKPQWLRAKAPTGEVFNETLNIVKLHNLHTVCEEAACPNIGECWNKRHATVMILGSVCTRACAFCNVATGIPDKLDPH
EPENLAKAIKKLNLKHVVITSVDRDDLPDGGANQFIQCIEEIRKITSETTIEILTPDFLNKKGAFEAIAVASPDVYNHNI
ETVPRLYAKIRPRARYFHSLYLLKMVKQINPKVFTKSGLMVGLGETKEEIFQVMDDLRSAEVDFITIGQYLQPTPKHAKL
DRYVTPEEFEHYKYIAYSKGFLVVASSPLTRSSYHAEEDFNRLKACR

Sequences:

>Translated_287_residues
MHSKPQWLRAKAPTGEVFNETLNIVKLHNLHTVCEEAACPNIGECWNKRHATVMILGSVCTRACAFCNVATGIPDKLDPH
EPENLAKAIKKLNLKHVVITSVDRDDLPDGGANQFIQCIEEIRKITSETTIEILTPDFLNKKGAFEAIAVASPDVYNHNI
ETVPRLYAKIRPRARYFHSLYLLKMVKQINPKVFTKSGLMVGLGETKEEIFQVMDDLRSAEVDFITIGQYLQPTPKHAKL
DRYVTPEEFEHYKYIAYSKGFLVVASSPLTRSSYHAEEDFNRLKACR
>Mature_287_residues
MHSKPQWLRAKAPTGEVFNETLNIVKLHNLHTVCEEAACPNIGECWNKRHATVMILGSVCTRACAFCNVATGIPDKLDPH
EPENLAKAIKKLNLKHVVITSVDRDDLPDGGANQFIQCIEEIRKITSETTIEILTPDFLNKKGAFEAIAVASPDVYNHNI
ETVPRLYAKIRPRARYFHSLYLLKMVKQINPKVFTKSGLMVGLGETKEEIFQVMDDLRSAEVDFITIGQYLQPTPKHAKL
DRYVTPEEFEHYKYIAYSKGFLVVASSPLTRSSYHAEEDFNRLKACR

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=283, Percent_Identity=46.643109540636, Blast_Score=276, Evalue=1e-74,
Organism=Homo sapiens, GI37577164, Length=244, Percent_Identity=46.7213114754098, Blast_Score=239, Evalue=2e-63,
Organism=Escherichia coli, GI1786846, Length=276, Percent_Identity=49.6376811594203, Blast_Score=278, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI32564533, Length=254, Percent_Identity=47.6377952755905, Blast_Score=252, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6324770, Length=283, Percent_Identity=46.643109540636, Blast_Score=261, Evalue=1e-70,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=46.9964664310954, Blast_Score=256, Evalue=1e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 32531; Mature: 32531

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSKPQWLRAKAPTGEVFNETLNIVKLHNLHTVCEEAACPNIGECWNKRHATVMILGSVC
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEHHHHH
TRACAFCNVATGIPDKLDPHEPENLAKAIKKLNLKHVVITSVDRDDLPDGGANQFIQCIE
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHH
EIRKITSETTIEILTPDFLNKKGAFEAIAVASPDVYNHNIETVPRLYAKIRPRARYFHSL
HHHHHHCCCEEEEECHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHH
YLLKMVKQINPKVFTKSGLMVGLGETKEEIFQVMDDLRSAEVDFITIGQYLQPTPKHAKL
HHHHHHHHCCCCEEECCCCEEECCCCHHHHHHHHHHHHHCCCCEEECCHHHCCCCCCCCC
DRYVTPEEFEHYKYIAYSKGFLVVASSPLTRSSYHAEEDFNRLKACR
CCCCCHHHHHHEEEEEEECCEEEEECCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MHSKPQWLRAKAPTGEVFNETLNIVKLHNLHTVCEEAACPNIGECWNKRHATVMILGSVC
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEHHHHH
TRACAFCNVATGIPDKLDPHEPENLAKAIKKLNLKHVVITSVDRDDLPDGGANQFIQCIE
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHH
EIRKITSETTIEILTPDFLNKKGAFEAIAVASPDVYNHNIETVPRLYAKIRPRARYFHSL
HHHHHHCCCEEEEECHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHH
YLLKMVKQINPKVFTKSGLMVGLGETKEEIFQVMDDLRSAEVDFITIGQYLQPTPKHAKL
HHHHHHHHCCCCEEECCCCEEECCCCHHHHHHHHHHHHHCCCCEEECCHHHCCCCCCCCC
DRYVTPEEFEHYKYIAYSKGFLVVASSPLTRSSYHAEEDFNRLKACR
CCCCCHHHHHHEEEEEEECCEEEEECCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA