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The map label for this gene is ubiE [H]

Identifier: 225630195

GI number: 225630195

Start: 425438

End: 426154

Strand: Reverse

Name: ubiE [H]

Synonym: WRi_003960

Alternate gene names: 225630195

Gene position: 426154-425438 (Counterclockwise)

Preceding gene: 225630196

Following gene: 225630194

Centisome position: 29.47

GC content: 34.59

Gene sequence:

>717_bases
ATGTCTACTATAAAAATCGAGAAGAAATCACAATTAGTTAAAGAGGTATTCGATTCCGTGGCAAGTCGCTACGACACCAT
GAATGATATAATGAGCCTCGGAATGCACAGATTATGGAAAGATAGGATGGTAAATAGTGTGCATTTTACAAAAAACTCTA
AGGTTTTAGATGTTGCTGGAGGAACTGGAGATATAGCAATAAGAATAGTAAGAAAAGAGCCAAGTGCTAAGGTTACAGTA
TGCGATATAAATCAAAACATGCTAAGCAGAGGACGTGATAAAGCTATAAATTCAAACCAAATTAATTTTGATTGGGTATG
TGCGAACGCAGAAAGTTTACCATTTGAAGACTCCGAATTTGATTATTGCACAATAGCTTTTGGCATTCGAAATGTTTCTG
ACCGTAAGAAGGCTTTAAATGAGGCGCACAGGGTATTAAAACCACATGGAAAATTTATCTGCTTAGAATTTGCCCCTATG
CACTATCAAAATGAGATATTTACCAAACTTTATGACTTATATTCATTTAAAGTAATTCCTAAAATTGGCAGCATAGTTGC
TAAAGACAAGAGTTCTTATGAATATTTAGTGAAGAGCATCAGAGAGTTTCCAACTCAGGCTGATTTTAAAATGGAAATTG
AAGAGGTAGGCTTTAAGAATGTTGAGTTTCATAATATGAGCTATGGAATAGTGGCATTACACATTGGAACAAAATGA

Upstream 100 bases:

>100_bases
CATACCACGCTGAAGAAGATTTTAACAGGCTCAAGGCCTGTCGTTGACATTTGTGTTAATTCAAATTAATTTTTACTAAT
TATAAAGGAGTTTTTACGTA

Downstream 100 bases:

>100_bases
ATATTAAACTCTGGTATAGAACGCTGGATTATTATCTCATCCTTCCAGTGTTTTTTTTGCTCACTATAAGCTTCATTCTT
GTTTATTCAGCAAGCCCTGT

Product: ubiquinone/menaquinone biosynthesis methlytransferase UbiE

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MSTIKIEKKSQLVKEVFDSVASRYDTMNDIMSLGMHRLWKDRMVNSVHFTKNSKVLDVAGGTGDIAIRIVRKEPSAKVTV
CDINQNMLSRGRDKAINSNQINFDWVCANAESLPFEDSEFDYCTIAFGIRNVSDRKKALNEAHRVLKPHGKFICLEFAPM
HYQNEIFTKLYDLYSFKVIPKIGSIVAKDKSSYEYLVKSIREFPTQADFKMEIEEVGFKNVEFHNMSYGIVALHIGTK

Sequences:

>Translated_238_residues
MSTIKIEKKSQLVKEVFDSVASRYDTMNDIMSLGMHRLWKDRMVNSVHFTKNSKVLDVAGGTGDIAIRIVRKEPSAKVTV
CDINQNMLSRGRDKAINSNQINFDWVCANAESLPFEDSEFDYCTIAFGIRNVSDRKKALNEAHRVLKPHGKFICLEFAPM
HYQNEIFTKLYDLYSFKVIPKIGSIVAKDKSSYEYLVKSIREFPTQADFKMEIEEVGFKNVEFHNMSYGIVALHIGTK
>Mature_237_residues
STIKIEKKSQLVKEVFDSVASRYDTMNDIMSLGMHRLWKDRMVNSVHFTKNSKVLDVAGGTGDIAIRIVRKEPSAKVTVC
DINQNMLSRGRDKAINSNQINFDWVCANAESLPFEDSEFDYCTIAFGIRNVSDRKKALNEAHRVLKPHGKFICLEFAPMH
YQNEIFTKLYDLYSFKVIPKIGSIVAKDKSSYEYLVKSIREFPTQADFKMEIEEVGFKNVEFHNMSYGIVALHIGTK

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=271, Percent_Identity=45.3874538745387, Blast_Score=241, Evalue=6e-64,
Organism=Escherichia coli, GI48994983, Length=239, Percent_Identity=47.6987447698745, Blast_Score=225, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17557039, Length=234, Percent_Identity=50, Blast_Score=240, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6323526, Length=253, Percent_Identity=42.2924901185771, Blast_Score=185, Evalue=4e-48,
Organism=Drosophila melanogaster, GI24641702, Length=251, Percent_Identity=46.6135458167331, Blast_Score=236, Evalue=7e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27265; Mature: 27134

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS01183 UBIE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIKIEKKSQLVKEVFDSVASRYDTMNDIMSLGMHRLWKDRMVNSVHFTKNSKVLDVAG
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEEEEEC
GTGDIAIRIVRKEPSAKVTVCDINQNMLSRGRDKAINSNQINFDWVCANAESLPFEDSEF
CCCCEEEEEEECCCCCEEEEEECCHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCC
DYCTIAFGIRNVSDRKKALNEAHRVLKPHGKFICLEFAPMHYQNEIFTKLYDLYSFKVIP
CEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHEEHH
KIGSIVAKDKSSYEYLVKSIREFPTQADFKMEIEEVGFKNVEFHNMSYGIVALHIGTK
HHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEHHHCCCCCEEEECCCEEEEEEEECCC
>Mature Secondary Structure 
STIKIEKKSQLVKEVFDSVASRYDTMNDIMSLGMHRLWKDRMVNSVHFTKNSKVLDVAG
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEEEEEC
GTGDIAIRIVRKEPSAKVTVCDINQNMLSRGRDKAINSNQINFDWVCANAESLPFEDSEF
CCCCEEEEEEECCCCCEEEEEECCHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCC
DYCTIAFGIRNVSDRKKALNEAHRVLKPHGKFICLEFAPMHYQNEIFTKLYDLYSFKVIP
CEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHEEHH
KIGSIVAKDKSSYEYLVKSIREFPTQADFKMEIEEVGFKNVEFHNMSYGIVALHIGTK
HHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEHHHCCCCCEEEECCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA