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The map label for this gene is tsf [H]

Identifier: 225630156

GI number: 225630156

Start: 370075

End: 370935

Strand: Reverse

Name: tsf [H]

Synonym: WRi_003450

Alternate gene names: 225630156

Gene position: 370935-370075 (Counterclockwise)

Preceding gene: 225630157

Following gene: 225630155

Centisome position: 25.65

GC content: 36.0

Gene sequence:

>861_bases
ATGAAGATGAATCCAGATGATATAAGAGAATTACGTGATAGAACAGGGCTTGGCTTAAGTGACTGTAAAAAAGCATTAGA
AGAATGTGATGGTGACATTAAGAAAGCCGTTGATAAGTTACGTACAATAGGTCTTGCTAAAGCTGACAAAAAATCTGATA
GAGTAGCTTCAGATGGGCTCGTTGCTATGTGTTTGACTGAAAATTGTGGTGTATTGATTGAACTCAATTGCGAAACCGAT
TTTGTCGCAAGGAATGAGAAATTTATAGAGTTAGTTTTAAATTTAGCATCAATTGCTCATCAAGAACGCTGTACTAGCGT
TGATGAGTTAAAAAATGCCAAGTATGAAAGCATTGGCACAGTGCAGGAAGCTATTATGAATGGTACGTCAGTTCTTGGTG
AGAAGCTAGAGTTAAGCAAGCTTTGTTACTTAGAAGCTAAGGATGGAGTTATTGCTGGCTACGTACATGGTGATGTGTGT
GGCTTAGGTAAGATTGGTGCTTTAATTGCATTGCAATCACCCGGTGATAAGGCAAAGTTACAGGAAATTGGAAAACAAAT
AGCGATGCACATAGTTGCTATGAAGCCTGAAGCTTTGTCTATAGATGATTTAGATCAAATGAAGTTGAAAAATGAACGTT
CTATAATTGAAGAACAAGTAAGGAGCTTAAATAAACCTGAAGAAGTAGCAAAAAAAATAGTAGATGGACGAATGGCTAAG
TACTATGAAGAAGTTGTTTTACTGGAACAAAAGTTCATAAAAGATGATAAAATGAAGGTTTCTGATTTTATAAAATCAAG
CGAGGTGAGTGCTGTTAAACTCTCTAATTACAAGTTACTTGTTTTGGGTGGTGCAAACTAA

Upstream 100 bases:

>100_bases
AGGGGTGATGAGTTTATTCAAGAAAAAGAAGATGGTATTGTGCAAACTAAAAGGAGACGTAGTAAAGTTTACAAAGAAGA
AGAAAGGGAGGTAGTAACAA

Downstream 100 bases:

>100_bases
ATGGTAAAAAATGTTTCCCTCAATAAATGAAAAAAGCGAAATAAAATATTCCAGAGTGTTATTTAAAATTTCTGGTGAAG
CTTTAATGGGGTCACAGTCT

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKMNPDDIRELRDRTGLGLSDCKKALEECDGDIKKAVDKLRTIGLAKADKKSDRVASDGLVAMCLTENCGVLIELNCETD
FVARNEKFIELVLNLASIAHQERCTSVDELKNAKYESIGTVQEAIMNGTSVLGEKLELSKLCYLEAKDGVIAGYVHGDVC
GLGKIGALIALQSPGDKAKLQEIGKQIAMHIVAMKPEALSIDDLDQMKLKNERSIIEEQVRSLNKPEEVAKKIVDGRMAK
YYEEVVLLEQKFIKDDKMKVSDFIKSSEVSAVKLSNYKLLVLGGAN

Sequences:

>Translated_286_residues
MKMNPDDIRELRDRTGLGLSDCKKALEECDGDIKKAVDKLRTIGLAKADKKSDRVASDGLVAMCLTENCGVLIELNCETD
FVARNEKFIELVLNLASIAHQERCTSVDELKNAKYESIGTVQEAIMNGTSVLGEKLELSKLCYLEAKDGVIAGYVHGDVC
GLGKIGALIALQSPGDKAKLQEIGKQIAMHIVAMKPEALSIDDLDQMKLKNERSIIEEQVRSLNKPEEVAKKIVDGRMAK
YYEEVVLLEQKFIKDDKMKVSDFIKSSEVSAVKLSNYKLLVLGGAN
>Mature_286_residues
MKMNPDDIRELRDRTGLGLSDCKKALEECDGDIKKAVDKLRTIGLAKADKKSDRVASDGLVAMCLTENCGVLIELNCETD
FVARNEKFIELVLNLASIAHQERCTSVDELKNAKYESIGTVQEAIMNGTSVLGEKLELSKLCYLEAKDGVIAGYVHGDVC
GLGKIGALIALQSPGDKAKLQEIGKQIAMHIVAMKPEALSIDDLDQMKLKNERSIIEEQVRSLNKPEEVAKKIVDGRMAK
YYEEVVLLEQKFIKDDKMKVSDFIKSSEVSAVKLSNYKLLVLGGAN

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Homo sapiens, GI171846268, Length=285, Percent_Identity=28.4210526315789, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI291084500, Length=307, Percent_Identity=26.0586319218241, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI291084498, Length=91, Percent_Identity=45.0549450549451, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI291084502, Length=93, Percent_Identity=44.0860215053763, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1786366, Length=258, Percent_Identity=34.4961240310078, Blast_Score=141, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17561440, Length=288, Percent_Identity=28.8194444444444, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI19921466, Length=222, Percent_Identity=31.5315315315315, Blast_Score=95, Evalue=4e-20,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 31495; Mature: 31495

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS01126 EF_TS_1 ; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMNPDDIRELRDRTGLGLSDCKKALEECDGDIKKAVDKLRTIGLAKADKKSDRVASDGL
CCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCE
VAMCLTENCGVLIELNCETDFVARNEKFIELVLNLASIAHQERCTSVDELKNAKYESIGT
EEEEEECCCCEEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHH
VQEAIMNGTSVLGEKLELSKLCYLEAKDGVIAGYVHGDVCGLGKIGALIALQSPGDKAKL
HHHHHHCCHHHHHHHHHHHHHEEEECCCCEEEEEECCCHHCCCCHHHEEEECCCCCHHHH
QEIGKQIAMHIVAMKPEALSIDDLDQMKLKNERSIIEEQVRSLNKPEEVAKKIVDGRMAK
HHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
YYEEVVLLEQKFIKDDKMKVSDFIKSSEVSAVKLSNYKLLVLGGAN
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCEEEEEEECCC
>Mature Secondary Structure
MKMNPDDIRELRDRTGLGLSDCKKALEECDGDIKKAVDKLRTIGLAKADKKSDRVASDGL
CCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCE
VAMCLTENCGVLIELNCETDFVARNEKFIELVLNLASIAHQERCTSVDELKNAKYESIGT
EEEEEECCCCEEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHH
VQEAIMNGTSVLGEKLELSKLCYLEAKDGVIAGYVHGDVCGLGKIGALIALQSPGDKAKL
HHHHHHCCHHHHHHHHHHHHHEEEECCCCEEEEEECCCHHCCCCHHHEEEECCCCCHHHH
QEIGKQIAMHIVAMKPEALSIDDLDQMKLKNERSIIEEQVRSLNKPEEVAKKIVDGRMAK
HHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
YYEEVVLLEQKFIKDDKMKVSDFIKSSEVSAVKLSNYKLLVLGGAN
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA