Click here to switch to the map view.

The map label for this gene is miaB [H]

Identifier: 225630141

GI number: 225630141

Start: 351525

End: 352844

Strand: Reverse

Name: miaB [H]

Synonym: WRi_003230

Alternate gene names: 225630141

Gene position: 352844-351525 (Counterclockwise)

Preceding gene: 225630142

Following gene: 225630139

Centisome position: 24.4

GC content: 39.17

Gene sequence:

>1320_bases
ATGAAAGGCCTATATATTAAAACTTACGGCTGTCAGATGAACGTTTATGACTCCGTTTTAATGGAAAATGTAATTAAACC
TTTGGGGTTCAACGTTGTTAGTGATGCAGGGAAAGCCGATTTAGTGATACTGAATACTTGTCACATTAGAGAGAAGGCAG
CAGAAAAACTTTACTCAGAGCTTGGGAAGATTCATTCGTTACGAAAAGAAATGACTATAGTGGTGGCTGGGTGTGTAGCG
CAAGCAGAGGGAGAAGAAGTTTTCAGAAGGGCTCCTTTTGTTGATATTGTTGTTGGTCCGCAGAGTATCGCTACCTTACC
GGAACTGATAGTCAAAGCAAGCAGAAGTAAAGGTCATGTAATAAACACTGATTTTCCTGAAGTTGCAAAGTTTGATAAAT
TGCCAGATGAATGCTATGGCAATAGCCAGGGGTCTTCTGCGTTTCTTGCCATACAAGAGGGTTGTGATAAATTTTGTACG
TTTTGTGTAGTGCCCTATACTCGTGGAGCTGAGTATTCACGGCCAGTGAATGAAATATTTCGTGAAGCATTGAAATTAGT
TGCAAATGGGGCAAATGAGATCAATTTGCTTGGTCAGAACGTCAATGCTTACCATGGGGAGTGCGAAGGGGAAGTGTGGG
ATTTAGGAAAATTAATTAGTCATATTGCTAAAATTGAAAAGCTAGAGAGGATTCGTTATACAACTTCTCATCCAAGAGAT
ATGCATGAATCTCTCTACTTGGCACATGCGGAAGAGCCAAAGCTCATGCCGTTTGTTCACCTGCCTGTGCAGTCTGGTTC
AAATAAAATATTGCACGCGATGAACAGGAAACACACTGCAGAGGAGTATTTGGAAATAATAGACAGATTTCGCAAATTGA
AACCTGAAATCGAATTTTCTTCTGATTTTATTGTTGGTTTTCCTGGAGAAACCGAAAAAGATTTTGAAGAAACTATGAAA
TTAGTAGAAAAAGTTAGATATGCTCAGGCTTATAGTTTTAAATACAGCCCAAGACCAGGCACACCGGGAGCGGAAAGAAA
AGATCAAGTACCAGAAGAGGTTAAAACAGAGCGCCTTCTTCGTTTACAGAAATTAATTAGTAAGCAACAACTTGAGTTTA
ACCAGAGTATGGTAGGTAAAACTATACCTGTTCTATTTAGCGATAAAAAAGGTAAACACCAAAACCAAATTATTGGTAAA
AGCCCATATATGCAATCAGTGTGCATTGATGATTCTGAAGATAAATACAGGGACAAAATAGTAAATGTGAAAGTGTTGGA
AGCTCGGCAGAGTAGTTTGCTGGGGTGTGCATTTCACTGA

Upstream 100 bases:

>100_bases
TGTCAAAATCTATTGTGCTAGCAAGTTCACTAGCGTCACGCGCTGGTCTGTTAGACATTTACGTGTAGGGTGAAGAAATT
TTGAAAAAGACATACCAAAT

Downstream 100 bases:

>100_bases
TTTTAAGCAGCGTTAAATATAAATCTTAAGCAAAGAGTATTGAAAAAATGCAATCATAATCTTTTTCAAAAGTTACAAAG
TTTAATTGCTACCTCTGCTG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MKGLYIKTYGCQMNVYDSVLMENVIKPLGFNVVSDAGKADLVILNTCHIREKAAEKLYSELGKIHSLRKEMTIVVAGCVA
QAEGEEVFRRAPFVDIVVGPQSIATLPELIVKASRSKGHVINTDFPEVAKFDKLPDECYGNSQGSSAFLAIQEGCDKFCT
FCVVPYTRGAEYSRPVNEIFREALKLVANGANEINLLGQNVNAYHGECEGEVWDLGKLISHIAKIEKLERIRYTTSHPRD
MHESLYLAHAEEPKLMPFVHLPVQSGSNKILHAMNRKHTAEEYLEIIDRFRKLKPEIEFSSDFIVGFPGETEKDFEETMK
LVEKVRYAQAYSFKYSPRPGTPGAERKDQVPEEVKTERLLRLQKLISKQQLEFNQSMVGKTIPVLFSDKKGKHQNQIIGK
SPYMQSVCIDDSEDKYRDKIVNVKVLEARQSSLLGCAFH

Sequences:

>Translated_439_residues
MKGLYIKTYGCQMNVYDSVLMENVIKPLGFNVVSDAGKADLVILNTCHIREKAAEKLYSELGKIHSLRKEMTIVVAGCVA
QAEGEEVFRRAPFVDIVVGPQSIATLPELIVKASRSKGHVINTDFPEVAKFDKLPDECYGNSQGSSAFLAIQEGCDKFCT
FCVVPYTRGAEYSRPVNEIFREALKLVANGANEINLLGQNVNAYHGECEGEVWDLGKLISHIAKIEKLERIRYTTSHPRD
MHESLYLAHAEEPKLMPFVHLPVQSGSNKILHAMNRKHTAEEYLEIIDRFRKLKPEIEFSSDFIVGFPGETEKDFEETMK
LVEKVRYAQAYSFKYSPRPGTPGAERKDQVPEEVKTERLLRLQKLISKQQLEFNQSMVGKTIPVLFSDKKGKHQNQIIGK
SPYMQSVCIDDSEDKYRDKIVNVKVLEARQSSLLGCAFH
>Mature_439_residues
MKGLYIKTYGCQMNVYDSVLMENVIKPLGFNVVSDAGKADLVILNTCHIREKAAEKLYSELGKIHSLRKEMTIVVAGCVA
QAEGEEVFRRAPFVDIVVGPQSIATLPELIVKASRSKGHVINTDFPEVAKFDKLPDECYGNSQGSSAFLAIQEGCDKFCT
FCVVPYTRGAEYSRPVNEIFREALKLVANGANEINLLGQNVNAYHGECEGEVWDLGKLISHIAKIEKLERIRYTTSHPRD
MHESLYLAHAEEPKLMPFVHLPVQSGSNKILHAMNRKHTAEEYLEIIDRFRKLKPEIEFSSDFIVGFPGETEKDFEETMK
LVEKVRYAQAYSFKYSPRPGTPGAERKDQVPEEVKTERLLRLQKLISKQQLEFNQSMVGKTIPVLFSDKKGKHQNQIIGK
SPYMQSVCIDDSEDKYRDKIVNVKVLEARQSSLLGCAFH

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=450, Percent_Identity=33.3333333333333, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI28872782, Length=450, Percent_Identity=33.3333333333333, Blast_Score=255, Evalue=6e-68,
Organism=Homo sapiens, GI93277076, Length=387, Percent_Identity=27.390180878553, Blast_Score=166, Evalue=4e-41,
Organism=Escherichia coli, GI1786882, Length=439, Percent_Identity=44.1913439635535, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1787057, Length=440, Percent_Identity=30, Blast_Score=183, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17553146, Length=414, Percent_Identity=34.0579710144928, Blast_Score=237, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI71996771, Length=286, Percent_Identity=29.3706293706294, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI21356207, Length=431, Percent_Identity=30.8584686774942, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI19922432, Length=459, Percent_Identity=26.797385620915, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49627; Mature: 49627

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGLYIKTYGCQMNVYDSVLMENVIKPLGFNVVSDAGKADLVILNTCHIREKAAEKLYSE
CCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEEEHHHHHHHHHHHHHH
LGKIHSLRKEMTIVVAGCVAQAEGEEVFRRAPFVDIVVGPQSIATLPELIVKASRSKGHV
HHHHHHHHHHHHHHEEHHHHHCCHHHHHHHCCEEEEEECCHHHHHHHHHHHHHCCCCCCE
INTDFPEVAKFDKLPDECYGNSQGSSAFLAIQEGCDKFCTFCVVPYTRGAEYSRPVNEIF
ECCCCHHHHHHHCCCHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
REALKLVANGANEINLLGQNVNAYHGECEGEVWDLGKLISHIAKIEKLERIRYTTSHPRD
HHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
MHESLYLAHAEEPKLMPFVHLPVQSGSNKILHAMNRKHTAEEYLEIIDRFRKLKPEIEFS
HHHHHEEEECCCCCCCCEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEC
SDFIVGFPGETEKDFEETMKLVEKVRYAQAYSFKYSPRPGTPGAERKDQVPEEVKTERLL
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCHHCCHHHHHHHHHH
RLQKLISKQQLEFNQSMVGKTIPVLFSDKKGKHQNQIIGKSPYMQSVCIDDSEDKYRDKI
HHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHH
VNVKVLEARQSSLLGCAFH
CCEEEEEHHHHCCCCCCCC
>Mature Secondary Structure
MKGLYIKTYGCQMNVYDSVLMENVIKPLGFNVVSDAGKADLVILNTCHIREKAAEKLYSE
CCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEEEHHHHHHHHHHHHHH
LGKIHSLRKEMTIVVAGCVAQAEGEEVFRRAPFVDIVVGPQSIATLPELIVKASRSKGHV
HHHHHHHHHHHHHHEEHHHHHCCHHHHHHHCCEEEEEECCHHHHHHHHHHHHHCCCCCCE
INTDFPEVAKFDKLPDECYGNSQGSSAFLAIQEGCDKFCTFCVVPYTRGAEYSRPVNEIF
ECCCCHHHHHHHCCCHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
REALKLVANGANEINLLGQNVNAYHGECEGEVWDLGKLISHIAKIEKLERIRYTTSHPRD
HHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
MHESLYLAHAEEPKLMPFVHLPVQSGSNKILHAMNRKHTAEEYLEIIDRFRKLKPEIEFS
HHHHHEEEECCCCCCCCEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEC
SDFIVGFPGETEKDFEETMKLVEKVRYAQAYSFKYSPRPGTPGAERKDQVPEEVKTERLL
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCHHCCHHHHHHHHHH
RLQKLISKQQLEFNQSMVGKTIPVLFSDKKGKHQNQIIGKSPYMQSVCIDDSEDKYRDKI
HHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHH
VNVKVLEARQSSLLGCAFH
CCEEEEEHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA