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The map label for this gene is gatA [H]

Identifier: 225630068

GI number: 225630068

Start: 251411

End: 252880

Strand: Reverse

Name: gatA [H]

Synonym: WRi_002430

Alternate gene names: 225630068

Gene position: 252880-251411 (Counterclockwise)

Preceding gene: 225630071

Following gene: 225630065

Centisome position: 17.49

GC content: 39.8

Gene sequence:

>1470_bases
ATGAATGAACTAAGAAAGTTAAGTATTACACAAATGCATGACGGGCTCAAAAAAAAGAGTTTTTCTGCCGTTGAACTCGT
AGAGGCGCACATCAGTGCGGTTGAAAATGAGAAATTAAATGCATTTATAACAAAAACCCCAGAGATAGCAATGAAAGCTG
CTAAAGCTGCAGATGAACATTTCTCTAGGCAAAAGGATGATTTAATCTCGCCGCTCATGGGTGTACCAGTTGGCATCAAA
GATCTATTCTGTACAAAAGGAATAAAAACAACAGCATGCTCAAAAATGCTGGAAAATTTCGTTCCGACTTACGAATCTAC
GGTGTCTGACTTGCTTTTAAAAAGTGGAGCAGCCATGCTCGGTAAGCTTAATATGGATGAATTTGCTATGGGTTCTGCGA
ACACAAATAGCTATTTTGGCCCTGTTGAAAATGTGTGGATTAGAAAAAGCGATGGGGAAAAAGTTGTTCCTGGTGGATCA
TCTGGTGGATCTGCAGCATCGGTTGCTGGATTTTTATGCGCTGGAGCGTTAGGGAGCGACACCGGTGGATCTGTACGCCA
ACCAGCAGCTTATTGTGGAGTGGTTGGAATAAAGCCAACTTATGGAAGATGCTCGCGTTTTGGTATGATTGCATTTGCGA
GTTCTCTGGATCAAGCAGGAGTCATTACTCGTTCTGTCTCTGATTCAGCATTAATGCTGGAAGCAATTTGTGGCTATGAT
ACAAAAGATTCAATATCGAGCGAAAAGCCAGTGCCTAAACTTTCTAGCTTTATAAATGGTGATGTCAAGGGTAAGTGTAT
TGGTATACCAAAAGAATATAGGATGGATGGAATTTCAGAGGAAATAGTTCATAATTGGGAAAGAGTTGCTTCCTATTTAA
AAGAAAATGGCGCTGAAGTTGTTGACATTACTCTGCCACATACTAAATATGCAATACCAGTCTATTATTTAATCTGTTCT
GCTGAAACTTCATCCAATCTTGCTCGTTACGATGGTGTGCGTTATGGATTAAGGGTTGATGCTGATACCCTTGAAGAAAT
GTATTCACTAACAAGAGCAGAAGGTTTCGGTAAAGAGGTGAAAAGAAGAATTTTAATTGGCGCTTATGCGCTTTCCTCAG
GTCATTACAATGAATATTACGAAAAAGCACAGTGTATCAGGGCATTAATCAGAAATGATTTTATAAAAGCGTTTGAAAAA
ATAGATTACATACTTGTGCCATCTGCTCCAACAGAAGCTTTTGGCTTAAATGAAAAGCCAGATCCGCTAATTATGTGCAT
CAATGATGTGTTTACTGTGCCGGCAAGTCTGGCAGGATTGCCTGCTATTTCTGTTCCTGTTGGGCTCTCCAATGAGGGTT
TACCACTTGCCTTGCAAGTGATTGGAAATTATTATGATGAAGCTGGAATATTAAATGTGGCAAGTGTGATAGAGCAAAAT
TGCGGCAGAATAATTAGACCTCTTGCATAA

Upstream 100 bases:

>100_bases
ATCTAAGTTACCTTAGCTACATTTCTAGCTGTAACTATAAATGTATGGATTTTTTATCGTGCCATGTTATAATTAACGTC
TTGCTAAAAATTAAATATTG

Downstream 100 bases:

>100_bases
TCTATTTTTTGATAGTAATTTTTGCATTAAAATTTGCTTACGCTCCTCAACGTACTCTATGGTTCCATTTTTAGATGAAT
AGCAAAAACAGGGTATATTA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: ADP; phosphate; Gln-tRNA(Gln); L-glutamate

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MNELRKLSITQMHDGLKKKSFSAVELVEAHISAVENEKLNAFITKTPEIAMKAAKAADEHFSRQKDDLISPLMGVPVGIK
DLFCTKGIKTTACSKMLENFVPTYESTVSDLLLKSGAAMLGKLNMDEFAMGSANTNSYFGPVENVWIRKSDGEKVVPGGS
SGGSAASVAGFLCAGALGSDTGGSVRQPAAYCGVVGIKPTYGRCSRFGMIAFASSLDQAGVITRSVSDSALMLEAICGYD
TKDSISSEKPVPKLSSFINGDVKGKCIGIPKEYRMDGISEEIVHNWERVASYLKENGAEVVDITLPHTKYAIPVYYLICS
AETSSNLARYDGVRYGLRVDADTLEEMYSLTRAEGFGKEVKRRILIGAYALSSGHYNEYYEKAQCIRALIRNDFIKAFEK
IDYILVPSAPTEAFGLNEKPDPLIMCINDVFTVPASLAGLPAISVPVGLSNEGLPLALQVIGNYYDEAGILNVASVIEQN
CGRIIRPLA

Sequences:

>Translated_489_residues
MNELRKLSITQMHDGLKKKSFSAVELVEAHISAVENEKLNAFITKTPEIAMKAAKAADEHFSRQKDDLISPLMGVPVGIK
DLFCTKGIKTTACSKMLENFVPTYESTVSDLLLKSGAAMLGKLNMDEFAMGSANTNSYFGPVENVWIRKSDGEKVVPGGS
SGGSAASVAGFLCAGALGSDTGGSVRQPAAYCGVVGIKPTYGRCSRFGMIAFASSLDQAGVITRSVSDSALMLEAICGYD
TKDSISSEKPVPKLSSFINGDVKGKCIGIPKEYRMDGISEEIVHNWERVASYLKENGAEVVDITLPHTKYAIPVYYLICS
AETSSNLARYDGVRYGLRVDADTLEEMYSLTRAEGFGKEVKRRILIGAYALSSGHYNEYYEKAQCIRALIRNDFIKAFEK
IDYILVPSAPTEAFGLNEKPDPLIMCINDVFTVPASLAGLPAISVPVGLSNEGLPLALQVIGNYYDEAGILNVASVIEQN
CGRIIRPLA
>Mature_489_residues
MNELRKLSITQMHDGLKKKSFSAVELVEAHISAVENEKLNAFITKTPEIAMKAAKAADEHFSRQKDDLISPLMGVPVGIK
DLFCTKGIKTTACSKMLENFVPTYESTVSDLLLKSGAAMLGKLNMDEFAMGSANTNSYFGPVENVWIRKSDGEKVVPGGS
SGGSAASVAGFLCAGALGSDTGGSVRQPAAYCGVVGIKPTYGRCSRFGMIAFASSLDQAGVITRSVSDSALMLEAICGYD
TKDSISSEKPVPKLSSFINGDVKGKCIGIPKEYRMDGISEEIVHNWERVASYLKENGAEVVDITLPHTKYAIPVYYLICS
AETSSNLARYDGVRYGLRVDADTLEEMYSLTRAEGFGKEVKRRILIGAYALSSGHYNEYYEKAQCIRALIRNDFIKAFEK
IDYILVPSAPTEAFGLNEKPDPLIMCINDVFTVPASLAGLPAISVPVGLSNEGLPLALQVIGNYYDEAGILNVASVIEQN
CGRIIRPLA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=497, Percent_Identity=42.2535211267606, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI195972892, Length=500, Percent_Identity=25.8, Blast_Score=136, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17543272, Length=451, Percent_Identity=37.2505543237251, Blast_Score=291, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI17537465, Length=503, Percent_Identity=26.441351888668, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17556264, Length=309, Percent_Identity=27.831715210356, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=221, Percent_Identity=30.7692307692308, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=325, Percent_Identity=27.0769230769231, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=325, Percent_Identity=27.0769230769231, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17538252, Length=205, Percent_Identity=28.780487804878, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=431, Percent_Identity=37.8190255220418, Blast_Score=239, Evalue=6e-64,
Organism=Saccharomyces cerevisiae, GI6319685, Length=426, Percent_Identity=28.4037558685446, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6320448, Length=208, Percent_Identity=26.9230769230769, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24648113, Length=494, Percent_Identity=42.3076923076923, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24652985, Length=500, Percent_Identity=25.6, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI19922090, Length=500, Percent_Identity=25.6, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24652981, Length=500, Percent_Identity=25.6, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24652983, Length=500, Percent_Identity=25.6, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24644968, Length=500, Percent_Identity=25, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI21356731, Length=504, Percent_Identity=23.8095238095238, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI161078093, Length=515, Percent_Identity=25.0485436893204, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI45550774, Length=192, Percent_Identity=29.1666666666667, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24648435, Length=192, Percent_Identity=29.1666666666667, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24648437, Length=192, Percent_Identity=29.1666666666667, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.7

Molecular weight: Translated: 52766; Mature: 52766

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELRKLSITQMHDGLKKKSFSAVELVEAHISAVENEKLNAFITKTPEIAMKAAKAADEH
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHH
FSRQKDDLISPLMGVPVGIKDLFCTKGIKTTACSKMLENFVPTYESTVSDLLLKSGAAML
HHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHH
GKLNMDEFAMGSANTNSYFGPVENVWIRKSDGEKVVPGGSSGGSAASVAGFLCAGALGSD
CCCCHHHHCCCCCCCCCCCCCCCCEEEEECCCCEECCCCCCCCCHHHHHHHHHHCCCCCC
TGGSVRQPAAYCGVVGIKPTYGRCSRFGMIAFASSLDQAGVITRSVSDSALMLEAICGYD
CCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHCCC
TKDSISSEKPVPKLSSFINGDVKGKCIGIPKEYRMDGISEEIVHNWERVASYLKENGAEV
CCCCCCCCCCCHHHHHHHCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCEE
VDITLPHTKYAIPVYYLICSAETSSNLARYDGVRYGLRVDADTLEEMYSLTRAEGFGKEV
EEEECCCCCCEEEEEEEEEECCCCCCHHHHCCEEECEEECHHHHHHHHHHHHHHCHHHHH
KRRILIGAYALSSGHYNEYYEKAQCIRALIRNDFIKAFEKIDYILVPSAPTEAFGLNEKP
HHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHCCCCCCC
DPLIMCINDVFTVPASLAGLPAISVPVGLSNEGLPLALQVIGNYYDEAGILNVASVIEQN
CCEEEEHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
CGRIIRPLA
HHHHHCCCC
>Mature Secondary Structure
MNELRKLSITQMHDGLKKKSFSAVELVEAHISAVENEKLNAFITKTPEIAMKAAKAADEH
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHH
FSRQKDDLISPLMGVPVGIKDLFCTKGIKTTACSKMLENFVPTYESTVSDLLLKSGAAML
HHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHH
GKLNMDEFAMGSANTNSYFGPVENVWIRKSDGEKVVPGGSSGGSAASVAGFLCAGALGSD
CCCCHHHHCCCCCCCCCCCCCCCCEEEEECCCCEECCCCCCCCCHHHHHHHHHHCCCCCC
TGGSVRQPAAYCGVVGIKPTYGRCSRFGMIAFASSLDQAGVITRSVSDSALMLEAICGYD
CCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHCCC
TKDSISSEKPVPKLSSFINGDVKGKCIGIPKEYRMDGISEEIVHNWERVASYLKENGAEV
CCCCCCCCCCCHHHHHHHCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCEE
VDITLPHTKYAIPVYYLICSAETSSNLARYDGVRYGLRVDADTLEEMYSLTRAEGFGKEV
EEEECCCCCCEEEEEEEEEECCCCCCHHHHCCEEECEEECHHHHHHHHHHHHHHCHHHHH
KRRILIGAYALSSGHYNEYYEKAQCIRALIRNDFIKAFEKIDYILVPSAPTEAFGLNEKP
HHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHCCCCCCC
DPLIMCINDVFTVPASLAGLPAISVPVGLSNEGLPLALQVIGNYYDEAGILNVASVIEQN
CCEEEEHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
CGRIIRPLA
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; Glu-tRNA(Gln); L-glutamine

Specific reaction: ATP + Glu-tRNA(Gln) + L-glutamine = ADP + phosphate + Gln-tRNA(Gln) + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA