Click here to switch to the map view.

The map label for this gene is mdtM [H]

Identifier: 225630065

GI number: 225630065

Start: 243628

End: 244791

Strand: Reverse

Name: mdtM [H]

Synonym: WRi_002350

Alternate gene names: 225630065

Gene position: 244791-243628 (Counterclockwise)

Preceding gene: 225630068

Following gene: 225630063

Centisome position: 16.93

GC content: 36.77

Gene sequence:

>1164_bases
ATGTTACTACCCTTTCTATTAATCCTGTCTCTAATTGCCAAGTTCATTGAAATTGACATTTCCGTACCTAGTTTTCCTGA
TATGGTGCACTATTTTAACGTATCAGAAGGAACAATTCAATTAACTATTGCTTATAATTTTTTAGGCTTTTGCATAGGAG
GGTTGTTTTTTGGTCCATTGTCTGAATGCTATGGCAGAAGGAGAATTATGATCATAGGTAACACTTTGTTATTAATCGGC
GCTGTTGGTTGTGTTTTTGCACCGTCGATTTTTTGGCTTTTAATCTCTCGCTTTGTTCAGGGCATTGGTGTTAGCACATC
TGTAGTTGTGTTTGCAATCGTTGCAGACAGCTACAAAGGCGACGAAGCGGTGAAATTTATTGGAATCATGAATTCTGTTC
TTACAGTTGTCATGGCAATTGCGCCAGTGTTAGGCAGTTTCATCAATGAAATTGTAGGATGGCGTGGTAATTATGCAACT
GTTGCAATACTTTGTTTAATCTCTTGGGTTTTGCTGCTTTTAGCGTTACCAGAAACAAAAAAGGACCGCGATATTTTCAG
TTTGAAAAAAATGATGAAAGATTACAGAAAACTACTATCTAGTCCGAGGTTTGTTACGTTGTCTTTGATGTCAAGCCTCT
TTTCTGCTGCGTATATGTCATTCATTACTTGTGGACCTTTTCTGTATATGAAGACTTTTGGTTTATCTAGCACTATTTAT
GCACTACATCAAGGTGCAATAGTTGGGTGCTTCTCACTCATCAGTCTATTTGCTGGCAAAATCTTGCAGAAACTTGGAGC
AATAAAGTGTGTAATTTCTGGTACGAGCGTGGGTGCTATTGGGTCTCTATTGCTTGTAATATTCAGTATAATTGTACCCC
ACTCTTTCTACTTAGTAACACTGTCTATGATTATTTTTTCTATCGGTTGTGCAATTTTCCAGCCAGTGATTTTTAATGCA
TCAATAAATGTTTTCCCTGAAATTAAAGGCACAGCTTCTTCTGCAGTCTCGTTCATTAGAGCTTTTATCATGGCTATTTT
TATCGGCTTAACAAGCTACATTTACAATGGTCAAACAATTAGTGTAGCTGTTCTTGTTTTATCCGCGATAGTACTAGAAC
TTGTTTTTACTGTCTATCTATTGCGTTTAAAAGATCCTATGTAA

Upstream 100 bases:

>100_bases
CTTCACATGAGGTGTTTCTAACCATTTTAATAACAGTAAAAAATGTTCTTCTTTCAACGCTTTAAATGTGATATTTTTAT
TCATTAATTGAAAATCTCAA

Downstream 100 bases:

>100_bases
TTAATAAAGATATTCCATTGTAGGCTGCTAACTTTCCCCGGCCAATCTTTCTGCTTCATTGCGTGAAATTAATGCCTCAA
TAAATTCATCTAGTTCACCT

Product: drug resistance transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MLLPFLLILSLIAKFIEIDISVPSFPDMVHYFNVSEGTIQLTIAYNFLGFCIGGLFFGPLSECYGRRRIMIIGNTLLLIG
AVGCVFAPSIFWLLISRFVQGIGVSTSVVVFAIVADSYKGDEAVKFIGIMNSVLTVVMAIAPVLGSFINEIVGWRGNYAT
VAILCLISWVLLLLALPETKKDRDIFSLKKMMKDYRKLLSSPRFVTLSLMSSLFSAAYMSFITCGPFLYMKTFGLSSTIY
ALHQGAIVGCFSLISLFAGKILQKLGAIKCVISGTSVGAIGSLLLVIFSIIVPHSFYLVTLSMIIFSIGCAIFQPVIFNA
SINVFPEIKGTASSAVSFIRAFIMAIFIGLTSYIYNGQTISVAVLVLSAIVLELVFTVYLLRLKDPM

Sequences:

>Translated_387_residues
MLLPFLLILSLIAKFIEIDISVPSFPDMVHYFNVSEGTIQLTIAYNFLGFCIGGLFFGPLSECYGRRRIMIIGNTLLLIG
AVGCVFAPSIFWLLISRFVQGIGVSTSVVVFAIVADSYKGDEAVKFIGIMNSVLTVVMAIAPVLGSFINEIVGWRGNYAT
VAILCLISWVLLLLALPETKKDRDIFSLKKMMKDYRKLLSSPRFVTLSLMSSLFSAAYMSFITCGPFLYMKTFGLSSTIY
ALHQGAIVGCFSLISLFAGKILQKLGAIKCVISGTSVGAIGSLLLVIFSIIVPHSFYLVTLSMIIFSIGCAIFQPVIFNA
SINVFPEIKGTASSAVSFIRAFIMAIFIGLTSYIYNGQTISVAVLVLSAIVLELVFTVYLLRLKDPM
>Mature_387_residues
MLLPFLLILSLIAKFIEIDISVPSFPDMVHYFNVSEGTIQLTIAYNFLGFCIGGLFFGPLSECYGRRRIMIIGNTLLLIG
AVGCVFAPSIFWLLISRFVQGIGVSTSVVVFAIVADSYKGDEAVKFIGIMNSVLTVVMAIAPVLGSFINEIVGWRGNYAT
VAILCLISWVLLLLALPETKKDRDIFSLKKMMKDYRKLLSSPRFVTLSLMSSLFSAAYMSFITCGPFLYMKTFGLSSTIY
ALHQGAIVGCFSLISLFAGKILQKLGAIKCVISGTSVGAIGSLLLVIFSIIVPHSFYLVTLSMIIFSIGCAIFQPVIFNA
SINVFPEIKGTASSAVSFIRAFIMAIFIGLTSYIYNGQTISVAVLVLSAIVLELVFTVYLLRLKDPM

Specific function: Confers resistance to acriflavine, chloramphenicol, norfloxacin, ethidium bromide and TPP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1790794, Length=383, Percent_Identity=22.976501305483, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1788509, Length=347, Percent_Identity=24.4956772334294, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1787065, Length=364, Percent_Identity=23.9010989010989, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI48994889, Length=377, Percent_Identity=23.6074270557029, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1790146, Length=225, Percent_Identity=25.7777777777778, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87082312, Length=329, Percent_Identity=22.4924012158055, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=163, Percent_Identity=26.3803680981595, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42208; Mature: 42208

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLPFLLILSLIAKFIEIDISVPSFPDMVHYFNVSEGTIQLTIAYNFLGFCIGGLFFGPL
CHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEECCCCEEEEEEHHHHHHHHHHHHHHHHH
SECYGRRRIMIIGNTLLLIGAVGCVFAPSIFWLLISRFVQGIGVSTSVVVFAIVADSYKG
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
DEAVKFIGIMNSVLTVVMAIAPVLGSFINEIVGWRGNYATVAILCLISWVLLLLALPETK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCC
KDRDIFSLKKMMKDYRKLLSSPRFVTLSLMSSLFSAAYMSFITCGPFLYMKTFGLSSTIY
CCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ALHQGAIVGCFSLISLFAGKILQKLGAIKCVISGTSVGAIGSLLLVIFSIIVPHSFYLVT
HHHCCHHHHHHHHHHHHHHHHHHHHCHHEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHH
LSMIIFSIGCAIFQPVIFNASINVFPEIKGTASSAVSFIRAFIMAIFIGLTSYIYNGQTI
HHHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
SVAVLVLSAIVLELVFTVYLLRLKDPM
HHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLLPFLLILSLIAKFIEIDISVPSFPDMVHYFNVSEGTIQLTIAYNFLGFCIGGLFFGPL
CHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEECCCCEEEEEEHHHHHHHHHHHHHHHHH
SECYGRRRIMIIGNTLLLIGAVGCVFAPSIFWLLISRFVQGIGVSTSVVVFAIVADSYKG
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
DEAVKFIGIMNSVLTVVMAIAPVLGSFINEIVGWRGNYATVAILCLISWVLLLLALPETK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCC
KDRDIFSLKKMMKDYRKLLSSPRFVTLSLMSSLFSAAYMSFITCGPFLYMKTFGLSSTIY
CCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ALHQGAIVGCFSLISLFAGKILQKLGAIKCVISGTSVGAIGSLLLVIFSIIVPHSFYLVT
HHHCCHHHHHHHHHHHHHHHHHHHHCHHEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHH
LSMIIFSIGCAIFQPVIFNASINVFPEIKGTASSAVSFIRAFIMAIFIGLTSYIYNGQTI
HHHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
SVAVLVLSAIVLELVFTVYLLRLKDPM
HHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]