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The map label for this gene is ispG [H]

Identifier: 225629987

GI number: 225629987

Start: 135312

End: 136592

Strand: Direct

Name: ispG [H]

Synonym: WRi_001360

Alternate gene names: 225629987

Gene position: 135312-136592 (Clockwise)

Preceding gene: 225629986

Following gene: 225629992

Centisome position: 9.36

GC content: 37.78

Gene sequence:

>1281_bases
ATGCTAGATAAAGACCTGATATTGAATGATAATACATATGAATTATCGCAGATTTCCAGGCACAAAACTCATGTTGTAGA
AGTTGGAAAAGTAAAGATAGGCGGAAATAATCCTGTAGTTGTACAATCTATGGCGCTTGGCGTGCATATAGATGCTGATA
ATATAAAAAGTAGCGCAAAACATTATGCAAAAGAGATAACAGAACTAGCGCGTACAGGTTCAGAATTGGTGCGAATTGCC
TTGAACTCAGAGGAGGTAGCAAGAGCAATACCTTATATAGTAGAAGAGATAAATAAAGAAGGTTTTGACGGTAAAATATT
GGTAGGCTGCGGTCAATATGAGCTAGATAAATTAGTTCAAGATTATCCAGACAACATCAAAATGCTGGGTAAAATTAGAA
TCAATCCAGGCAATATAGGCTTTGGCGACAAACGTGATGAGAAGTTTGAAAGGGTGATAGAGTATGCAATAATGCACGAT
CTTCCGGTTAGAATTGGGGTAAATTGGGGTAGTCTTGATAAATACCTTTTGCAAAAATTGATGGATGAAAACTCTTCGCT
TAGTAATTCAAGGCCTTCTGATGTTATACTGCGCAAAGCACTTGTAATGTCTGCTCTTGATAGTGCAAAAAAAGCTGAAG
GAATTGGTCTAAATTCAAACAAAATAATCATTTCATGTAAAGTTAGCAAAGTGCAAGATTTAATTTTAGTTTATATGGCA
CTTGCAAAATCTTCCAATTATGCGCTGCATTTGGGTTTAACTGAGGCTGGTATGGGCAATAAAGGTGTGGTAAATACCGC
AGCAGGGCTTACTTATTTATTGCAAAATGGCATTGGAGATACAATACGGGCTTCTTTGACTCAACGTCCTGGTGAATCGC
GTACTAATGAAGTGGTGGTGTGTCAGGAAATATTGCAGTCTATAGGCTTGCGATACTTTAACCCTCAGGTTAATTCATGT
CCTGGTTGTGGGCGCACGAGTAGCGATCGTTTTCGTATATTAACTGAAGAGGTGAATGGCTATATAAAAACTCATATACC
GATATGGAAGAAAAAAAATCCAGGTGTAGAGCATATGAGCATTGCTGTTATGGGATGCATAGTAAACGGTCCTGGAGAAA
GCAAACACGCAAATTTGGGAATCAGCTTGCCTGGATATGGAGAAAAACCTGTTTCAGCAGTCTACAAAGACGGCAAATAT
TTCAAAACTTTACAAGGTGATAATGTCTCTGAAGAATTTAAGGCAATTATTGATAATTATGTGAAGGAGCATTACACGTA
A

Upstream 100 bases:

>100_bases
AATTTGGATAACGTAGATGCTTATAGTTTGAATGGTTCAAGTTCATTTGGCAGTGTAGCTGACACAGTAAAATAGTATCA
CAATATTGGCCATTAATAAT

Downstream 100 bases:

>100_bases
CCTTTCACGGTTAATTAATAGATAAATCGCAAATAAAAAAGAAAATTAGACCTTGTTTTTCTTTTTTCGTTGGGAATGTT
CAAAAAAGCATACGCGTCAA

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MLDKDLILNDNTYELSQISRHKTHVVEVGKVKIGGNNPVVVQSMALGVHIDADNIKSSAKHYAKEITELARTGSELVRIA
LNSEEVARAIPYIVEEINKEGFDGKILVGCGQYELDKLVQDYPDNIKMLGKIRINPGNIGFGDKRDEKFERVIEYAIMHD
LPVRIGVNWGSLDKYLLQKLMDENSSLSNSRPSDVILRKALVMSALDSAKKAEGIGLNSNKIIISCKVSKVQDLILVYMA
LAKSSNYALHLGLTEAGMGNKGVVNTAAGLTYLLQNGIGDTIRASLTQRPGESRTNEVVVCQEILQSIGLRYFNPQVNSC
PGCGRTSSDRFRILTEEVNGYIKTHIPIWKKKNPGVEHMSIAVMGCIVNGPGESKHANLGISLPGYGEKPVSAVYKDGKY
FKTLQGDNVSEEFKAIIDNYVKEHYT

Sequences:

>Translated_426_residues
MLDKDLILNDNTYELSQISRHKTHVVEVGKVKIGGNNPVVVQSMALGVHIDADNIKSSAKHYAKEITELARTGSELVRIA
LNSEEVARAIPYIVEEINKEGFDGKILVGCGQYELDKLVQDYPDNIKMLGKIRINPGNIGFGDKRDEKFERVIEYAIMHD
LPVRIGVNWGSLDKYLLQKLMDENSSLSNSRPSDVILRKALVMSALDSAKKAEGIGLNSNKIIISCKVSKVQDLILVYMA
LAKSSNYALHLGLTEAGMGNKGVVNTAAGLTYLLQNGIGDTIRASLTQRPGESRTNEVVVCQEILQSIGLRYFNPQVNSC
PGCGRTSSDRFRILTEEVNGYIKTHIPIWKKKNPGVEHMSIAVMGCIVNGPGESKHANLGISLPGYGEKPVSAVYKDGKY
FKTLQGDNVSEEFKAIIDNYVKEHYT
>Mature_426_residues
MLDKDLILNDNTYELSQISRHKTHVVEVGKVKIGGNNPVVVQSMALGVHIDADNIKSSAKHYAKEITELARTGSELVRIA
LNSEEVARAIPYIVEEINKEGFDGKILVGCGQYELDKLVQDYPDNIKMLGKIRINPGNIGFGDKRDEKFERVIEYAIMHD
LPVRIGVNWGSLDKYLLQKLMDENSSLSNSRPSDVILRKALVMSALDSAKKAEGIGLNSNKIIISCKVSKVQDLILVYMA
LAKSSNYALHLGLTEAGMGNKGVVNTAAGLTYLLQNGIGDTIRASLTQRPGESRTNEVVVCQEILQSIGLRYFNPQVNSC
PGCGRTSSDRFRILTEEVNGYIKTHIPIWKKKNPGVEHMSIAVMGCIVNGPGESKHANLGISLPGYGEKPVSAVYKDGKY
FKTLQGDNVSEEFKAIIDNYVKEHYT

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=405, Percent_Identity=30.3703703703704, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 46925; Mature: 46925

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDKDLILNDNTYELSQISRHKTHVVEVGKVKIGGNNPVVVQSMALGVHIDADNIKSSAK
CCCCCEEECCCCHHHHHHHHHHHEEEEEEEEEECCCCCEEEEEEEEEEEEEHHHHHHHHH
HYAKEITELARTGSELVRIALNSEEVARAIPYIVEEINKEGFDGKILVGCGQYELDKLVQ
HHHHHHHHHHHCHHHHEEEEECHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHH
DYPDNIKMLGKIRINPGNIGFGDKRDEKFERVIEYAIMHDLPVRIGVNWGSLDKYLLQKL
HCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHH
MDENSSLSNSRPSDVILRKALVMSALDSAKKAEGIGLNSNKIIISCKVSKVQDLILVYMA
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHH
LAKSSNYALHLGLTEAGMGNKGVVNTAAGLTYLLQNGIGDTIRASLTQRPGESRTNEVVV
HHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHH
CQEILQSIGLRYFNPQVNSCPGCGRTSSDRFRILTEEVNGYIKTHIPIWKKKNPGVEHMS
HHHHHHHCCCEEECCCCCCCCCCCCCCCCCEEEEEHHCCCEEEECCCEEECCCCCCCCEE
IAVMGCIVNGPGESKHANLGISLPGYGEKPVSAVYKDGKYFKTLQGDNVSEEFKAIIDNY
EEEEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHCCCCEEEEECCCCHHHHHHHHHHHH
VKEHYT
HHHCCC
>Mature Secondary Structure
MLDKDLILNDNTYELSQISRHKTHVVEVGKVKIGGNNPVVVQSMALGVHIDADNIKSSAK
CCCCCEEECCCCHHHHHHHHHHHEEEEEEEEEECCCCCEEEEEEEEEEEEEHHHHHHHHH
HYAKEITELARTGSELVRIALNSEEVARAIPYIVEEINKEGFDGKILVGCGQYELDKLVQ
HHHHHHHHHHHCHHHHEEEEECHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHH
DYPDNIKMLGKIRINPGNIGFGDKRDEKFERVIEYAIMHDLPVRIGVNWGSLDKYLLQKL
HCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHH
MDENSSLSNSRPSDVILRKALVMSALDSAKKAEGIGLNSNKIIISCKVSKVQDLILVYMA
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHH
LAKSSNYALHLGLTEAGMGNKGVVNTAAGLTYLLQNGIGDTIRASLTQRPGESRTNEVVV
HHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHH
CQEILQSIGLRYFNPQVNSCPGCGRTSSDRFRILTEEVNGYIKTHIPIWKKKNPGVEHMS
HHHHHHHCCCEEECCCCCCCCCCCCCCCCCEEEEEHHCCCEEEECCCEEECCCCCCCCEE
IAVMGCIVNGPGESKHANLGISLPGYGEKPVSAVYKDGKYFKTLQGDNVSEEFKAIIDNY
EEEEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHCCCCEEEEECCCCHHHHHHHHHHHH
VKEHYT
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA