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The map label for this gene is metK [H]

Identifier: 225629969

GI number: 225629969

Start: 117477

End: 118649

Strand: Reverse

Name: metK [H]

Synonym: WRi_001150

Alternate gene names: 225629969

Gene position: 118649-117477 (Counterclockwise)

Preceding gene: 225629970

Following gene: 225629968

Centisome position: 8.21

GC content: 37.17

Gene sequence:

>1173_bases
ATGAGTTTATATAAAAATTTAGTAACCAGTGAATCAGTGGCGGCTGGTCATCCAGATAAAGTAGCAGATCAAATTTCAGA
TGCAATACTTGATGAATACCTTTTCACTGATCCTTTTGCGCGAGCTGCAATAGAGACTTTAGTCACCAAAGATAACGTTA
TTATAGCTGGGGAAGTGTTTGGGCCTAACATCAAAAATAGCAGGATTGAAAGTATTGTACGGAATACAATAAAAGATATT
GGTTACGAGCACGATGGATTCCACTGGAGAAAAGTGAAAGTAAATATATTGCTACATGAGCAATCAAATGACATTGCAAT
AGGGTTGGATCAAGGTGCTGGAGATCAGGGCATAATGTATGGCTATGCAACAACAGAGACAAAAAACCTTATGCCGGCAC
CCATTTTTTATGCACACTCGATTCTGAAAAATATCATGAGCGCAGTTAAAGAAGCAAAACTTGGTCCAGATGCAAAGTCA
CAAATCACTTTGGCATATGAGAATAACCTCCCGGTACGTGCTGAAAGTATTATTGTCTCAATACAGCACCCTGAGGATTT
GGATCAATCAAAAGTAAAAGAAATAATTTACCCTTACATAGTTTCATCTTTACCTGAAGGATGGATGTGTCCTGAGGAAA
ATCTCTTAGTCAATCCAACTGGTAGATTTGTTATTGGTGGACCGGTTAGTGATTGTGGATTAACCGGACGAAAAATTATG
GTTGATACTTATGGAGGTTATATTCCACACGGTGGAGGAGCGTTTTCCGGAAAAGATGCAACCAAAGTTGATAGATCTGC
GGCTTATATGGCAAGATATCTTGCTAAAAATATTGTCTTTGCAGGTTTGGCTGAACGTTGTCTTGTGCAGCTTTCTTATG
CAATTGGTGTATCAAAACCTACTTCATTCTATATTGATACATTTGGTGCGAATACGGTAGAGGAAGGAGTAATTAAAAAG
TTTATCGAGAATAATATAGATTTATCAACAAAAGGCATAATCAAGCATTTATCGCTTAATCATCCTATATATAAACGTAC
AGCCTGTTATGGACATTTTGGTAAACAGTCGGAAAGGGACGGTGGATTTTCTTGGGAAAACATGGACTTATCTGCTGATC
TTTGTAGAGAATTCAATATAGAAGGTAATAGTAAAATTTCTTTAGATTGCTAA

Upstream 100 bases:

>100_bases
GATAAGCGTGAGGTCGGAGGTTCAACTCTTCCCAGACCCACCATTTAGTAGTTACTAACTGTGTTTTCTATAGCGGTTTT
GATATTGTATAAGCAAAAAC

Downstream 100 bases:

>100_bases
AATTTTTGATTTACAGCCCCATATTTATATACTAGCTTATTGTTAAAGCATAGCACGCTTTTTCATAATAATTATACCAC
ATTAGTAATAATTTTTATAT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MSLYKNLVTSESVAAGHPDKVADQISDAILDEYLFTDPFARAAIETLVTKDNVIIAGEVFGPNIKNSRIESIVRNTIKDI
GYEHDGFHWRKVKVNILLHEQSNDIAIGLDQGAGDQGIMYGYATTETKNLMPAPIFYAHSILKNIMSAVKEAKLGPDAKS
QITLAYENNLPVRAESIIVSIQHPEDLDQSKVKEIIYPYIVSSLPEGWMCPEENLLVNPTGRFVIGGPVSDCGLTGRKIM
VDTYGGYIPHGGGAFSGKDATKVDRSAAYMARYLAKNIVFAGLAERCLVQLSYAIGVSKPTSFYIDTFGANTVEEGVIKK
FIENNIDLSTKGIIKHLSLNHPIYKRTACYGHFGKQSERDGGFSWENMDLSADLCREFNIEGNSKISLDC

Sequences:

>Translated_390_residues
MSLYKNLVTSESVAAGHPDKVADQISDAILDEYLFTDPFARAAIETLVTKDNVIIAGEVFGPNIKNSRIESIVRNTIKDI
GYEHDGFHWRKVKVNILLHEQSNDIAIGLDQGAGDQGIMYGYATTETKNLMPAPIFYAHSILKNIMSAVKEAKLGPDAKS
QITLAYENNLPVRAESIIVSIQHPEDLDQSKVKEIIYPYIVSSLPEGWMCPEENLLVNPTGRFVIGGPVSDCGLTGRKIM
VDTYGGYIPHGGGAFSGKDATKVDRSAAYMARYLAKNIVFAGLAERCLVQLSYAIGVSKPTSFYIDTFGANTVEEGVIKK
FIENNIDLSTKGIIKHLSLNHPIYKRTACYGHFGKQSERDGGFSWENMDLSADLCREFNIEGNSKISLDC
>Mature_389_residues
SLYKNLVTSESVAAGHPDKVADQISDAILDEYLFTDPFARAAIETLVTKDNVIIAGEVFGPNIKNSRIESIVRNTIKDIG
YEHDGFHWRKVKVNILLHEQSNDIAIGLDQGAGDQGIMYGYATTETKNLMPAPIFYAHSILKNIMSAVKEAKLGPDAKSQ
ITLAYENNLPVRAESIIVSIQHPEDLDQSKVKEIIYPYIVSSLPEGWMCPEENLLVNPTGRFVIGGPVSDCGLTGRKIMV
DTYGGYIPHGGGAFSGKDATKVDRSAAYMARYLAKNIVFAGLAERCLVQLSYAIGVSKPTSFYIDTFGANTVEEGVIKKF
IENNIDLSTKGIIKHLSLNHPIYKRTACYGHFGKQSERDGGFSWENMDLSADLCREFNIEGNSKISLDC

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=378, Percent_Identity=44.7089947089947, Blast_Score=290, Evalue=1e-78,
Organism=Homo sapiens, GI5174529, Length=377, Percent_Identity=44.0318302387268, Blast_Score=287, Evalue=2e-77,
Organism=Escherichia coli, GI1789311, Length=387, Percent_Identity=50.1291989664083, Blast_Score=365, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17538494, Length=384, Percent_Identity=44.53125, Blast_Score=297, Evalue=5e-81,
Organism=Caenorhabditis elegans, GI17538498, Length=394, Percent_Identity=43.9086294416244, Blast_Score=297, Evalue=5e-81,
Organism=Caenorhabditis elegans, GI25145633, Length=384, Percent_Identity=44.2708333333333, Blast_Score=290, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI17551082, Length=380, Percent_Identity=43.421052631579, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI32565905, Length=382, Percent_Identity=41.6230366492147, Blast_Score=262, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI32565911, Length=342, Percent_Identity=42.3976608187134, Blast_Score=243, Evalue=9e-65,
Organism=Caenorhabditis elegans, GI25145635, Length=332, Percent_Identity=42.4698795180723, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI32565903, Length=299, Percent_Identity=44.1471571906354, Blast_Score=228, Evalue=4e-60,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=46.9879518072289, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320710, Length=380, Percent_Identity=43.6842105263158, Blast_Score=286, Evalue=3e-78,
Organism=Saccharomyces cerevisiae, GI6323209, Length=378, Percent_Identity=42.5925925925926, Blast_Score=276, Evalue=4e-75,
Organism=Drosophila melanogaster, GI24580527, Length=377, Percent_Identity=44.0318302387268, Blast_Score=284, Evalue=7e-77,
Organism=Drosophila melanogaster, GI24580525, Length=377, Percent_Identity=44.0318302387268, Blast_Score=284, Evalue=7e-77,
Organism=Drosophila melanogaster, GI45552159, Length=377, Percent_Identity=44.0318302387268, Blast_Score=280, Evalue=9e-76,
Organism=Drosophila melanogaster, GI24580529, Length=377, Percent_Identity=44.0318302387268, Blast_Score=280, Evalue=9e-76,
Organism=Drosophila melanogaster, GI24580537, Length=377, Percent_Identity=44.0318302387268, Blast_Score=280, Evalue=9e-76,
Organism=Drosophila melanogaster, GI24580533, Length=377, Percent_Identity=44.0318302387268, Blast_Score=280, Evalue=9e-76,
Organism=Drosophila melanogaster, GI24580535, Length=377, Percent_Identity=44.0318302387268, Blast_Score=280, Evalue=9e-76,
Organism=Drosophila melanogaster, GI24580531, Length=377, Percent_Identity=44.0318302387268, Blast_Score=280, Evalue=9e-76,
Organism=Drosophila melanogaster, GI24580541, Length=365, Percent_Identity=43.013698630137, Blast_Score=267, Evalue=9e-72,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=41.1764705882353, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42899; Mature: 42768

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLYKNLVTSESVAAGHPDKVADQISDAILDEYLFTDPFARAAIETLVTKDNVIIAGEVF
CCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECC
GPNIKNSRIESIVRNTIKDIGYEHDGFHWRKVKVNILLHEQSNDIAIGLDQGAGDQGIMY
CCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEECCCCEEEEECCCCCCCCEEE
GYATTETKNLMPAPIFYAHSILKNIMSAVKEAKLGPDAKSQITLAYENNLPVRAESIIVS
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEEEEEE
IQHPEDLDQSKVKEIIYPYIVSSLPEGWMCPEENLLVNPTGRFVIGGPVSDCGLTGRKIM
EECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEECCCCCCCCCCCCEEE
VDTYGGYIPHGGGAFSGKDATKVDRSAAYMARYLAKNIVFAGLAERCLVQLSYAIGVSKP
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TSFYIDTFGANTVEEGVIKKFIENNIDLSTKGIIKHLSLNHPIYKRTACYGHFGKQSERD
CEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCC
GGFSWENMDLSADLCREFNIEGNSKISLDC
CCCCCCCCCCCHHHHHHCCCCCCCEEEECC
>Mature Secondary Structure 
SLYKNLVTSESVAAGHPDKVADQISDAILDEYLFTDPFARAAIETLVTKDNVIIAGEVF
CHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECC
GPNIKNSRIESIVRNTIKDIGYEHDGFHWRKVKVNILLHEQSNDIAIGLDQGAGDQGIMY
CCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEECCCCEEEEECCCCCCCCEEE
GYATTETKNLMPAPIFYAHSILKNIMSAVKEAKLGPDAKSQITLAYENNLPVRAESIIVS
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEEEEEE
IQHPEDLDQSKVKEIIYPYIVSSLPEGWMCPEENLLVNPTGRFVIGGPVSDCGLTGRKIM
EECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEECCCCCCCCCCCCEEE
VDTYGGYIPHGGGAFSGKDATKVDRSAAYMARYLAKNIVFAGLAERCLVQLSYAIGVSKP
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TSFYIDTFGANTVEEGVIKKFIENNIDLSTKGIIKHLSLNHPIYKRTACYGHFGKQSERD
CEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCC
GGFSWENMDLSADLCREFNIEGNSKISLDC
CCCCCCCCCCCHHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA