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The map label for this gene is glyS [H]

Identifier: 225629952

GI number: 225629952

Start: 96370

End: 98487

Strand: Direct

Name: glyS [H]

Synonym: WRi_000970

Alternate gene names: 225629952

Gene position: 96370-98487 (Clockwise)

Preceding gene: 225629951

Following gene: 225629953

Centisome position: 6.67

GC content: 34.04

Gene sequence:

>2118_bases
ATGTCGTTGCAGTTGTTATTTGAGTGCCTTTCAGAAGAAATACCACCGAGAATGCAGAATTCAGCTACAGTTCAAGTTAA
GAGCTGTATTGCCAATGTTTTTAACAAAAATAATGTAAAATTTACATCAATGGAGGTTTTTATAACGGCACGTCGCATTA
CCCTATTTGTCGACAACATAAATGCTTTGGAGCTAAAGGATTCCAATAACGAAGTTAAGGGACCAAACATTAACGCACCA
AAAAGTGCTATCGAAGGTTTTTTAAGAAAATATCAGAAAAATGAAGAAGATTTGCTCGTTCGAAAAGTAAATAATGAGGA
TTTTTACTTCATTAAAAGAGAAAGCTGCTCATTTAACATCAGAGAATTCCTCAAAAATCAACTGGAGGAAATGCTCAAAA
ACTTTTCTTGGTTGAAGAGCATGAGATGGGGCGAAGGAAAAGAAAGGTGGGTTAGGCCAATCAAAAACATTTTATGCATT
TTAAATGACGAAATAATACCTGTGTCTTTTGCAGGGATCACAGCATCTAACACAACATATGGCCATCGATTTCTCTCAAG
TGGTACAGCGTTAACTGTTAAAGCACCTAAAGACTATTTTGAATTGCTAGAAAAAAACAGTGTAATTCTCCAAATGGACA
AAAGAAAGCAATTTATACTAGATCAGATTAATAAATTCACAAAAGAGCAGAATTTACAACTTGAGAAAAATGATTATTTA
CTAAATGAATTGACAGGGCTTATAGAGTGGCCAATCGTACTGTTTGGTGAAGTGAATCAAGAAAAGTCATTTGGATTACC
GAAGGAAGTAATACTTAGTATAGTTAATACACAACAAAAATACCTTGCTTTAAGTAATGGAAAGAGAATTTCACACTTTG
TCACTGTTGTCAACGTCAACAATGGTGAAGTTGTTAAAGGACACGAAAGAATATTAGAAGCACGTCTTGCTGATGCCCAA
TTTTTAATATCTCAAGATAAAAAGGAAAATCTAGATTATTATGTCAAAAAATTAGGTTCGATATTATTTCATGCTTCACT
TGGCAGCGTGGGGGAAAAGGTGAAGCGTATTACGGCTCTGTCAAAGTATATAGCTATATTTATTCCACATGCTTCGCTGA
TAAAAGTTGAACGCGCTGCATATTTGGCAAAAGCTGATTTGGCAACATCGATAGTAAGAGAGTTTCCAGAATTACAAGGA
GTAATGGGTGGATATTATGCTTCTTATTTTCAAGAAGATAGAGAAATAGTGGAAGCTATAACTGAGCATTATAAGCCAAT
CGGGCCAGAGCAAGAGTGTCCTAAATCTCCTTCTGCAATTGCTGTGTCAATTGCAGACAAAGTGGATAGCTTAGTTGGTT
TAATTGCAGCAGGTGAGAAAATCTCTGGTTCGTATGATCAGTTTGGTTTGCGGAGAATGACAATTGGTATCATTAGGACA
ATACTTGAAAATAATTTGCATATTCCAATTAGGCTAATGATAGATAAGTCAGTATCTTTATATTCAAGGCTTCTATTTAA
TAAAAATACAACATCAGTTGATAAGCCAAATAGAAAACAAATTTCAGAACTAGTATTTAGATTCTGCTTAGAAAGATTCA
AGGTTATTTTAAAAAATAGAGATATAAGGCAAGATATTGTAGATTCAATACTATATAAAATCGGTATTAATGATCTGCTG
ACAGCAGAAAAGCAAACTGTTATATTGGATCGTTATCTTAGTATGCCAGAAGGTGAACAGATTCTAAGCACTTACAAAAG
AGTCAGTAACATGATGAGCAAAGTAAGAAAAAGTGATGGCACTACTTATAGTGCATCTTACGGTAAGAGGTTTTTGATTG
AAAATGAAGAAATTGCGCTATCAAATTGTGCTATAACTGCTTGCAAAAACATAAAACAAGCAATAAAAAATAACCACTTT
AATGCAGCGCTTGATGAACTTGCTGGTTTTGCTCCGTTTATCAATCAGTTTATGGACAGTGTAAAGATTAACTGTGATTC
TGATAAGCTGAGAAGAAACAGATTATCTTTGCTTGAAAATGTAGTTTCCATCTTTCATTTAGTAGCAGATTTTAACCTCA
TACAGGTTAAGCAATGGATAAATGCTCAGGCAATATAA

Upstream 100 bases:

>100_bases
TGCAAGAGGTGTGCTTGGTGTGAATGAACGTACAGCTTATATTGGTAGAGTTAGAGAGCTGACAAAAAAATGTTGTGAAT
TGTACATGAGTAAATAGCGT

Downstream 100 bases:

>100_bases
AGAACAAATTAAATCATCTCCACAGTCCTGTGGCGTGTATAAGATGGTTGGAGATAAGAATAAAGTTTTATATGTTGGAA
AAGCAAAAAACTTAAAGTCA

Product: glycyl-tRNA synthetase, beta subunit

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 705; Mature: 704

Protein sequence:

>705_residues
MSLQLLFECLSEEIPPRMQNSATVQVKSCIANVFNKNNVKFTSMEVFITARRITLFVDNINALELKDSNNEVKGPNINAP
KSAIEGFLRKYQKNEEDLLVRKVNNEDFYFIKRESCSFNIREFLKNQLEEMLKNFSWLKSMRWGEGKERWVRPIKNILCI
LNDEIIPVSFAGITASNTTYGHRFLSSGTALTVKAPKDYFELLEKNSVILQMDKRKQFILDQINKFTKEQNLQLEKNDYL
LNELTGLIEWPIVLFGEVNQEKSFGLPKEVILSIVNTQQKYLALSNGKRISHFVTVVNVNNGEVVKGHERILEARLADAQ
FLISQDKKENLDYYVKKLGSILFHASLGSVGEKVKRITALSKYIAIFIPHASLIKVERAAYLAKADLATSIVREFPELQG
VMGGYYASYFQEDREIVEAITEHYKPIGPEQECPKSPSAIAVSIADKVDSLVGLIAAGEKISGSYDQFGLRRMTIGIIRT
ILENNLHIPIRLMIDKSVSLYSRLLFNKNTTSVDKPNRKQISELVFRFCLERFKVILKNRDIRQDIVDSILYKIGINDLL
TAEKQTVILDRYLSMPEGEQILSTYKRVSNMMSKVRKSDGTTYSASYGKRFLIENEEIALSNCAITACKNIKQAIKNNHF
NAALDELAGFAPFINQFMDSVKINCDSDKLRRNRLSLLENVVSIFHLVADFNLIQVKQWINAQAI

Sequences:

>Translated_705_residues
MSLQLLFECLSEEIPPRMQNSATVQVKSCIANVFNKNNVKFTSMEVFITARRITLFVDNINALELKDSNNEVKGPNINAP
KSAIEGFLRKYQKNEEDLLVRKVNNEDFYFIKRESCSFNIREFLKNQLEEMLKNFSWLKSMRWGEGKERWVRPIKNILCI
LNDEIIPVSFAGITASNTTYGHRFLSSGTALTVKAPKDYFELLEKNSVILQMDKRKQFILDQINKFTKEQNLQLEKNDYL
LNELTGLIEWPIVLFGEVNQEKSFGLPKEVILSIVNTQQKYLALSNGKRISHFVTVVNVNNGEVVKGHERILEARLADAQ
FLISQDKKENLDYYVKKLGSILFHASLGSVGEKVKRITALSKYIAIFIPHASLIKVERAAYLAKADLATSIVREFPELQG
VMGGYYASYFQEDREIVEAITEHYKPIGPEQECPKSPSAIAVSIADKVDSLVGLIAAGEKISGSYDQFGLRRMTIGIIRT
ILENNLHIPIRLMIDKSVSLYSRLLFNKNTTSVDKPNRKQISELVFRFCLERFKVILKNRDIRQDIVDSILYKIGINDLL
TAEKQTVILDRYLSMPEGEQILSTYKRVSNMMSKVRKSDGTTYSASYGKRFLIENEEIALSNCAITACKNIKQAIKNNHF
NAALDELAGFAPFINQFMDSVKINCDSDKLRRNRLSLLENVVSIFHLVADFNLIQVKQWINAQAI
>Mature_704_residues
SLQLLFECLSEEIPPRMQNSATVQVKSCIANVFNKNNVKFTSMEVFITARRITLFVDNINALELKDSNNEVKGPNINAPK
SAIEGFLRKYQKNEEDLLVRKVNNEDFYFIKRESCSFNIREFLKNQLEEMLKNFSWLKSMRWGEGKERWVRPIKNILCIL
NDEIIPVSFAGITASNTTYGHRFLSSGTALTVKAPKDYFELLEKNSVILQMDKRKQFILDQINKFTKEQNLQLEKNDYLL
NELTGLIEWPIVLFGEVNQEKSFGLPKEVILSIVNTQQKYLALSNGKRISHFVTVVNVNNGEVVKGHERILEARLADAQF
LISQDKKENLDYYVKKLGSILFHASLGSVGEKVKRITALSKYIAIFIPHASLIKVERAAYLAKADLATSIVREFPELQGV
MGGYYASYFQEDREIVEAITEHYKPIGPEQECPKSPSAIAVSIADKVDSLVGLIAAGEKISGSYDQFGLRRMTIGIIRTI
LENNLHIPIRLMIDKSVSLYSRLLFNKNTTSVDKPNRKQISELVFRFCLERFKVILKNRDIRQDIVDSILYKIGINDLLT
AEKQTVILDRYLSMPEGEQILSTYKRVSNMMSKVRKSDGTTYSASYGKRFLIENEEIALSNCAITACKNIKQAIKNNHFN
AALDELAGFAPFINQFMDSVKINCDSDKLRRNRLSLLENVVSIFHLVADFNLIQVKQWINAQAI

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=704, Percent_Identity=27.6988636363636, Blast_Score=286, Evalue=3e-78,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 80475; Mature: 80344

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQLLFECLSEEIPPRMQNSATVQVKSCIANVFNKNNVKFTSMEVFITARRITLFVDNI
CCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEEEEEEEEEECC
NALELKDSNNEVKGPNINAPKSAIEGFLRKYQKNEEDLLVRKVNNEDFYFIKRESCSFNI
CEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCH
REFLKNQLEEMLKNFSWLKSMRWGEGKERWVRPIKNILCILNDEIIPVSFAGITASNTTY
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECEEECCCCH
GHRFLSSGTALTVKAPKDYFELLEKNSVILQMDKRKQFILDQINKFTKEQNLQLEKNDYL
HHHHHCCCCEEEEECCHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHCCCEECCCCHH
LNELTGLIEWPIVLFGEVNQEKSFGLPKEVILSIVNTQQKYLALSNGKRISHFVTVVNVN
HHHHHHHHHCCHHEEECCCCCCCCCCCHHHHHHHHCCHHHHEEECCCCEEEEEEEEEECC
NGEVVKGHERILEARLADAQFLISQDKKENLDYYVKKLGSILFHASLGSVGEKVKRITAL
CCCEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKYIAIFIPHASLIKVERAAYLAKADLATSIVREFPELQGVMGGYYASYFQEDREIVEAI
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TEHYKPIGPEQECPKSPSAIAVSIADKVDSLVGLIAAGEKISGSYDQFGLRRMTIGIIRT
HHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
ILENNLHIPIRLMIDKSVSLYSRLLFNKNTTSVDKPNRKQISELVFRFCLERFKVILKNR
HHHCCCCEEEEEEECCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
DIRQDIVDSILYKIGINDLLTAEKQTVILDRYLSMPEGEQILSTYKRVSNMMSKVRKSDG
CHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
TTYSASYGKRFLIENEEIALSNCAITACKNIKQAIKNNHFNAALDELAGFAPFINQFMDS
CEEECCCCCEEEEECCCEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
VKINCDSDKLRRNRLSLLENVVSIFHLVADFNLIQVKQWINAQAI
CEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
SLQLLFECLSEEIPPRMQNSATVQVKSCIANVFNKNNVKFTSMEVFITARRITLFVDNI
CHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEEEEEEEEEECC
NALELKDSNNEVKGPNINAPKSAIEGFLRKYQKNEEDLLVRKVNNEDFYFIKRESCSFNI
CEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCH
REFLKNQLEEMLKNFSWLKSMRWGEGKERWVRPIKNILCILNDEIIPVSFAGITASNTTY
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECEEECCCCH
GHRFLSSGTALTVKAPKDYFELLEKNSVILQMDKRKQFILDQINKFTKEQNLQLEKNDYL
HHHHHCCCCEEEEECCHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHCCCEECCCCHH
LNELTGLIEWPIVLFGEVNQEKSFGLPKEVILSIVNTQQKYLALSNGKRISHFVTVVNVN
HHHHHHHHHCCHHEEECCCCCCCCCCCHHHHHHHHCCHHHHEEECCCCEEEEEEEEEECC
NGEVVKGHERILEARLADAQFLISQDKKENLDYYVKKLGSILFHASLGSVGEKVKRITAL
CCCEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKYIAIFIPHASLIKVERAAYLAKADLATSIVREFPELQGVMGGYYASYFQEDREIVEAI
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TEHYKPIGPEQECPKSPSAIAVSIADKVDSLVGLIAAGEKISGSYDQFGLRRMTIGIIRT
HHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
ILENNLHIPIRLMIDKSVSLYSRLLFNKNTTSVDKPNRKQISELVFRFCLERFKVILKNR
HHHCCCCEEEEEEECCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
DIRQDIVDSILYKIGINDLLTAEKQTVILDRYLSMPEGEQILSTYKRVSNMMSKVRKSDG
CHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
TTYSASYGKRFLIENEEIALSNCAITACKNIKQAIKNNHFNAALDELAGFAPFINQFMDS
CEEECCCCCEEEEECCCEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
VKINCDSDKLRRNRLSLLENVVSIFHLVADFNLIQVKQWINAQAI
CEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA