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The map label for this gene is hemB [H]

Identifier: 225629949

GI number: 225629949

Start: 93582

End: 94577

Strand: Direct

Name: hemB [H]

Synonym: WRi_000940

Alternate gene names: 225629949

Gene position: 93582-94577 (Clockwise)

Preceding gene: 225629948

Following gene: 225629950

Centisome position: 6.47

GC content: 35.24

Gene sequence:

>996_bases
ATGTTTAATTTCCCAAATACAAGATTAAGACGCAGGCGCTCGAGCAAATGGGTTCGCAATTTAACAAGTGAAAATAGTTT
ATCAGTAAATGATCTGGTTCTTCCTCTGTTTGTTCATGACAGAGAAGAAACAACTGAACCAATTTCTGGCTTACCAGATG
TAAAGTGTTATTCAATAGATGGATTAGTGTCTATAGTTAAGGAAGCTAAAGATTTAGGAATTAATGCTGTTGCAATTTTT
CCTGTAGTTGATAGTAAACTAAAATCTGAAAACGCTGAGGAAGCATATAATTCTGACAACTTAATCTGCAGAGCAATTTG
TGCTGTAAAATTAAAGGTACCTGAAATTGGTATTATTGCAGACATTGCATTGGATCCATACACTATTCATGGCCATGACG
GCATTTTAAAAGATAATCAGATGGATGTAGAAAACGATGAAACTATATCAGTACTGTGTAAGCAAGCACTTGCTTTAGCG
AAGGCAGGATGTGATATAGTTGCTCCTTCTGATATGATGGATGGTAGAATAGGAAGAATCAGAAAATCATTAGATGATAA
CAACTTTCAAGACGTATTAATATTATCTTATGCAGTGAAATACTGCTCTAGCTTCTATGCTCCATTCAGGCAAGTCGTTG
GTTCATGTGGGCTATCACATTCTATAGACAAAAGTGGTTATCAAATGGATTATAAAAATGCGCGCGAAGCAATGTGCGAA
ATTGAAATGGATATAAATGAAGGTGCAGACTTTATTATGATTAAACCAGGTATGCCATATTTAGATATTATCAAAACAGC
AAGTGATAAGTTTAATTTTCCGATTTTTGCTTACCAAGTAAGCGGTGAGTACGCAATGATAAAAGCTGCTGCAAATAATA
GCTGGCTGGATTATGACAAGGTGATTTATGAATCTTTGATTGGTTTCAAACGTGCGGGTGCAAGTGCAATATTCACTTAC
GCTGCACTTGATATTGCAAAAAATTTAAGTGCATAA

Upstream 100 bases:

>100_bases
CTAATGCGCCTTGGTTGGAAAGTATTTCTACCAATATCAGTGTTTTGGGTGATACTCATTTCAGGGGTGTTGCTCTTTAC
TGGGAATTTGCCTGGATCCA

Downstream 100 bases:

>100_bases
GCCCTATAAAATTATTAAATTTTTGGCGCACGTTCTTCTACTGTTGCTGCATATTACCTAAAATCTTCCAATACATGTTT
TAAGTTATTGATAAGTCCGT

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MFNFPNTRLRRRRSSKWVRNLTSENSLSVNDLVLPLFVHDREETTEPISGLPDVKCYSIDGLVSIVKEAKDLGINAVAIF
PVVDSKLKSENAEEAYNSDNLICRAICAVKLKVPEIGIIADIALDPYTIHGHDGILKDNQMDVENDETISVLCKQALALA
KAGCDIVAPSDMMDGRIGRIRKSLDDNNFQDVLILSYAVKYCSSFYAPFRQVVGSCGLSHSIDKSGYQMDYKNAREAMCE
IEMDINEGADFIMIKPGMPYLDIIKTASDKFNFPIFAYQVSGEYAMIKAAANNSWLDYDKVIYESLIGFKRAGASAIFTY
AALDIAKNLSA

Sequences:

>Translated_331_residues
MFNFPNTRLRRRRSSKWVRNLTSENSLSVNDLVLPLFVHDREETTEPISGLPDVKCYSIDGLVSIVKEAKDLGINAVAIF
PVVDSKLKSENAEEAYNSDNLICRAICAVKLKVPEIGIIADIALDPYTIHGHDGILKDNQMDVENDETISVLCKQALALA
KAGCDIVAPSDMMDGRIGRIRKSLDDNNFQDVLILSYAVKYCSSFYAPFRQVVGSCGLSHSIDKSGYQMDYKNAREAMCE
IEMDINEGADFIMIKPGMPYLDIIKTASDKFNFPIFAYQVSGEYAMIKAAANNSWLDYDKVIYESLIGFKRAGASAIFTY
AALDIAKNLSA
>Mature_331_residues
MFNFPNTRLRRRRSSKWVRNLTSENSLSVNDLVLPLFVHDREETTEPISGLPDVKCYSIDGLVSIVKEAKDLGINAVAIF
PVVDSKLKSENAEEAYNSDNLICRAICAVKLKVPEIGIIADIALDPYTIHGHDGILKDNQMDVENDETISVLCKQALALA
KAGCDIVAPSDMMDGRIGRIRKSLDDNNFQDVLILSYAVKYCSSFYAPFRQVVGSCGLSHSIDKSGYQMDYKNAREAMCE
IEMDINEGADFIMIKPGMPYLDIIKTASDKFNFPIFAYQVSGEYAMIKAAANNSWLDYDKVIYESLIGFKRAGASAIFTY
AALDIAKNLSA

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. Required for nodule development [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=301, Percent_Identity=36.2126245847176, Blast_Score=197, Evalue=1e-50,
Organism=Escherichia coli, GI87081728, Length=321, Percent_Identity=41.4330218068536, Blast_Score=229, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6321398, Length=309, Percent_Identity=36.2459546925566, Blast_Score=176, Evalue=4e-45,
Organism=Drosophila melanogaster, GI21358291, Length=295, Percent_Identity=35.5932203389831, Blast_Score=201, Evalue=6e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36736; Mature: 36736

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNFPNTRLRRRRSSKWVRNLTSENSLSVNDLVLPLFVHDREETTEPISGLPDVKCYSID
CCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEEEECCHHCCCCCCCCCCCEEEEHH
GLVSIVKEAKDLGINAVAIFPVVDSKLKSENAEEAYNSDNLICRAICAVKLKVPEIGIIA
HHHHHHHHHHHCCCCEEEEEEEHHHHHHCCCHHHHCCCCCHHEEEEEHEEEECCCCCEEE
DIALDPYTIHGHDGILKDNQMDVENDETISVLCKQALALAKAGCDIVAPSDMMDGRIGRI
EEECCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCHHHCCHHHHH
RKSLDDNNFQDVLILSYAVKYCSSFYAPFRQVVGSCGLSHSIDKSGYQMDYKNAREAMCE
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECHHHHHHHHEE
IEMDINEGADFIMIKPGMPYLDIIKTASDKFNFPIFAYQVSGEYAMIKAAANNSWLDYDK
EEEECCCCCCEEEECCCCCHHHHHHHCCCCCCCCEEEEEECCCEEEEEEECCCCCCHHHH
VIYESLIGFKRAGASAIFTYAALDIAKNLSA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFNFPNTRLRRRRSSKWVRNLTSENSLSVNDLVLPLFVHDREETTEPISGLPDVKCYSID
CCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEEEECCHHCCCCCCCCCCCEEEEHH
GLVSIVKEAKDLGINAVAIFPVVDSKLKSENAEEAYNSDNLICRAICAVKLKVPEIGIIA
HHHHHHHHHHHCCCCEEEEEEEHHHHHHCCCHHHHCCCCCHHEEEEEHEEEECCCCCEEE
DIALDPYTIHGHDGILKDNQMDVENDETISVLCKQALALAKAGCDIVAPSDMMDGRIGRI
EEECCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCHHHCCHHHHH
RKSLDDNNFQDVLILSYAVKYCSSFYAPFRQVVGSCGLSHSIDKSGYQMDYKNAREAMCE
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECHHHHHHHHEE
IEMDINEGADFIMIKPGMPYLDIIKTASDKFNFPIFAYQVSGEYAMIKAAANNSWLDYDK
EEEECCCCCCEEEECCCCCHHHHHHHCCCCCCCCEEEEEECCCEEEEEEECCCCCCHHHH
VIYESLIGFKRAGASAIFTYAALDIAKNLSA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226669; 12597275 [H]