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The map label for this gene is def [H]

Identifier: 225629944

GI number: 225629944

Start: 88054

End: 88593

Strand: Direct

Name: def [H]

Synonym: WRi_000890

Alternate gene names: 225629944

Gene position: 88054-88593 (Clockwise)

Preceding gene: 225629941

Following gene: 225629945

Centisome position: 6.09

GC content: 35.0

Gene sequence:

>540_bases
ATGTCCATATTACCAATTGTAATTGCCCCTGATGAAAGACTAATCACACGTGCCAGTGAAGTAACAGATATAAACGATAA
AATTAAAGAATTAGTAAACGACATGTTCGAAACTATGTACGATGCAGAAGGTCTTGGCCTTGCTGCAGTGCAAGTTGGAG
TGTTAAAGAGAATTTTTGTTGTGGATGTTCAGCTAGAAACAATTGAAAATGAGCCAGCAGGATATGGCTCAACTGGCAAA
TTTTACATGATTAATCCTGAGATTACAGAATTATCCGATGAACAAGTAATTCTCAAGGAAGGATGCCTTTCAATTCCAGA
GCAAAGTCATGAAATTAAGCGTCCAAAGTATCTAACTGTGAAATATAAAGACTTAGATAATGAAGAACAAACACTAAAAG
CTAGTGGTTGGCTTGCAAGGTGTATTCAGCATGAATTAGATCACTTAAACGGCATATTATATATTAGACATCTATCTAAA
TTAAAGTATGATATGGCGATAACAAAAGCACAGAAGATTAAAAAGCAACATGAGCAATGA

Upstream 100 bases:

>100_bases
TTTCTTTAATAATTTATATTTCTATTATAGATATAGCAACATTTTAATATACACTAAATTCTGGACAAAAGGAAAAGCAA
AACATAGAATTATAATTTTT

Downstream 100 bases:

>100_bases
AGACCTAGAATTATTAAAATTTTACCATGAAGTGGGCGTTGATTGCACACTAATGGAGGGTGAAGAGGAAAAAAAGTTGG
AGAGCAAAGAACCCTCTGTT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MSILPIVIAPDERLITRASEVTDINDKIKELVNDMFETMYDAEGLGLAAVQVGVLKRIFVVDVQLETIENEPAGYGSTGK
FYMINPEITELSDEQVILKEGCLSIPEQSHEIKRPKYLTVKYKDLDNEEQTLKASGWLARCIQHELDHLNGILYIRHLSK
LKYDMAITKAQKIKKQHEQ

Sequences:

>Translated_179_residues
MSILPIVIAPDERLITRASEVTDINDKIKELVNDMFETMYDAEGLGLAAVQVGVLKRIFVVDVQLETIENEPAGYGSTGK
FYMINPEITELSDEQVILKEGCLSIPEQSHEIKRPKYLTVKYKDLDNEEQTLKASGWLARCIQHELDHLNGILYIRHLSK
LKYDMAITKAQKIKKQHEQ
>Mature_178_residues
SILPIVIAPDERLITRASEVTDINDKIKELVNDMFETMYDAEGLGLAAVQVGVLKRIFVVDVQLETIENEPAGYGSTGKF
YMINPEITELSDEQVILKEGCLSIPEQSHEIKRPKYLTVKYKDLDNEEQTLKASGWLARCIQHELDHLNGILYIRHLSKL
KYDMAITKAQKIKKQHEQ

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=175, Percent_Identity=43.4285714285714, Blast_Score=130, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24645726, Length=158, Percent_Identity=29.1139240506329, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20443; Mature: 20312

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILPIVIAPDERLITRASEVTDINDKIKELVNDMFETMYDAEGLGLAAVQVGVLKRIFV
CCEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VDVQLETIENEPAGYGSTGKFYMINPEITELSDEQVILKEGCLSIPEQSHEIKRPKYLTV
EEEEEEECCCCCCCCCCCCEEEEECCCHHCCCCCCHHHHHHCCCCCCCCHHCCCCEEEEE
KYKDLDNEEQTLKASGWLARCIQHELDHLNGILYIRHLSKLKYDMAITKAQKIKKQHEQ
EECCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SILPIVIAPDERLITRASEVTDINDKIKELVNDMFETMYDAEGLGLAAVQVGVLKRIFV
CEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VDVQLETIENEPAGYGSTGKFYMINPEITELSDEQVILKEGCLSIPEQSHEIKRPKYLTV
EEEEEEECCCCCCCCCCCCEEEEECCCHHCCCCCCHHHHHHCCCCCCCCHHCCCCEEEEE
KYKDLDNEEQTLKASGWLARCIQHELDHLNGILYIRHLSKLKYDMAITKAQKIKKQHEQ
EECCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA