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The map label for this gene is proP [H]

Identifier: 225629941

GI number: 225629941

Start: 84487

End: 85734

Strand: Direct

Name: proP [H]

Synonym: WRi_000860

Alternate gene names: 225629941

Gene position: 84487-85734 (Clockwise)

Preceding gene: 225629938

Following gene: 225629944

Centisome position: 5.84

GC content: 34.29

Gene sequence:

>1248_bases
ATGCAGCAAATAACAAAAGTAATATTTTCAAGTTTGATTTGTAACACTTTATTGTGGTATGACCACATGCTTTTTGGCCA
CTTAATAAACATAATAGGTGGTGTGTTTTTTCCATCGGATGATCAACTAACCAGCGTATTGAAGGCTTTTGGAGTGTTTG
CAGTGGGTTTCTTAATGAGACCAGTCGGTGCTGCTATATTTGGTCACATTGGTGATAAACATGGCAGAAGAGTTGCCTTA
TTGCTCTCGATTATATTAATGACTTTATCAACCGTATTAATTGCTTTTGTTCCAGGTTATCAAAGCATTGGAATACTAGC
GTCAATGCTGGTAGTGTGTTTAAGATTATTGCAGGGTATATCTCTTGGAGGAGAAGCAGGAAATGCGCCATTTTTAATAG
AACATGCTCCTAAAAACAAAAAAGGATTTTTTGGCAGCATTGAAGTATTAAGCGCAATTTTTGGTTCAGTATTAAGCTTG
ATAGCGGTACTCATATGCAAAAAAGTATCTGATTTTGAATCTTGGGGGTGGAGATTACCTTTCATTTTCAGTTTAGCAAT
GGGATTAATCAGCATATATATGAGATATATACTTGATGAAAGCCCAGAGTATAAAAGACAAAAAAATCCGTCTAAATTGC
CATTAAAAGAGTTATTAAACAATTACAAAAAGCCTGTTCTAATATCAATAGGTATTGACTTGGTTGAAAATGCGTCTCTC
TATATATATTTGGTCTTTTTCAATGTGCTTACGAATAAAATATTAACAGTAAATACTCATTTTAATCATATAGTGGAAAT
ACTGAGTCAAATTGCACTTGGAGGATTGACAATATTGTTTGCAATACTTTCTGATAAGGTAGGAAGAGAAAAAGTTATGA
AGTTTGCATTTATAGCTTTTGTAATAGTGAGTTATCCAGTTTTTTCAATGTTATGTAGTGGCAATTACTTGCTGGCACAC
ATTCTTTTTATAATTCCAATAGCTGCATCGCTTGGTCCTGTCAGTGCACTTATGTGCGAATTATTTCCAACAAAGGTTCG
GTACAGTGGATTTGGTTTATCAAGAAATATAGCTGCTGGATTTTTTGGCGGCCTTGCACCATTTATATGCACAACAATTA
TCAAGGTTACAGGACAAGAGACTTCAGCTAGTTTTTACATGATTTTCTGTGCATTAATTGGGCTAGTTGCTATATCGCAA
ATTAAGGGTGAGCTTTCTTCAAAATCACCTAAAGTAAACCAAGTATAA

Upstream 100 bases:

>100_bases
GCAATAATATTAAAATCAAGGTTTAATATTGTTAATATAATATTCACATAACGTTCACATTTTTTTTTATATATAAGGTC
AAACAGGCTATAGGGAAATT

Downstream 100 bases:

>100_bases
TTGCAGTATAGCAGAATTTAAAAAAATCTAATTATAAGGTGGGAAATGCCAATTCTTAGGTGATAATGTGAATATTTCAG
CACCATCTTTTGTTACTCCA

Product: major facilitator family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MQQITKVIFSSLICNTLLWYDHMLFGHLINIIGGVFFPSDDQLTSVLKAFGVFAVGFLMRPVGAAIFGHIGDKHGRRVAL
LLSIILMTLSTVLIAFVPGYQSIGILASMLVVCLRLLQGISLGGEAGNAPFLIEHAPKNKKGFFGSIEVLSAIFGSVLSL
IAVLICKKVSDFESWGWRLPFIFSLAMGLISIYMRYILDESPEYKRQKNPSKLPLKELLNNYKKPVLISIGIDLVENASL
YIYLVFFNVLTNKILTVNTHFNHIVEILSQIALGGLTILFAILSDKVGREKVMKFAFIAFVIVSYPVFSMLCSGNYLLAH
ILFIIPIAASLGPVSALMCELFPTKVRYSGFGLSRNIAAGFFGGLAPFICTTIIKVTGQETSASFYMIFCALIGLVAISQ
IKGELSSKSPKVNQV

Sequences:

>Translated_415_residues
MQQITKVIFSSLICNTLLWYDHMLFGHLINIIGGVFFPSDDQLTSVLKAFGVFAVGFLMRPVGAAIFGHIGDKHGRRVAL
LLSIILMTLSTVLIAFVPGYQSIGILASMLVVCLRLLQGISLGGEAGNAPFLIEHAPKNKKGFFGSIEVLSAIFGSVLSL
IAVLICKKVSDFESWGWRLPFIFSLAMGLISIYMRYILDESPEYKRQKNPSKLPLKELLNNYKKPVLISIGIDLVENASL
YIYLVFFNVLTNKILTVNTHFNHIVEILSQIALGGLTILFAILSDKVGREKVMKFAFIAFVIVSYPVFSMLCSGNYLLAH
ILFIIPIAASLGPVSALMCELFPTKVRYSGFGLSRNIAAGFFGGLAPFICTTIIKVTGQETSASFYMIFCALIGLVAISQ
IKGELSSKSPKVNQV
>Mature_415_residues
MQQITKVIFSSLICNTLLWYDHMLFGHLINIIGGVFFPSDDQLTSVLKAFGVFAVGFLMRPVGAAIFGHIGDKHGRRVAL
LLSIILMTLSTVLIAFVPGYQSIGILASMLVVCLRLLQGISLGGEAGNAPFLIEHAPKNKKGFFGSIEVLSAIFGSVLSL
IAVLICKKVSDFESWGWRLPFIFSLAMGLISIYMRYILDESPEYKRQKNPSKLPLKELLNNYKKPVLISIGIDLVENASL
YIYLVFFNVLTNKILTVNTHFNHIVEILSQIALGGLTILFAILSDKVGREKVMKFAFIAFVIVSYPVFSMLCSGNYLLAH
ILFIIPIAASLGPVSALMCELFPTKVRYSGFGLSRNIAAGFFGGLAPFICTTIIKVTGQETSASFYMIFCALIGLVAISQ
IKGELSSKSPKVNQV

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=417, Percent_Identity=29.4964028776978, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1788292, Length=396, Percent_Identity=32.8282828282828, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1788942, Length=413, Percent_Identity=31.9612590799031, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1789941, Length=385, Percent_Identity=33.5064935064935, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI87082404, Length=386, Percent_Identity=27.2020725388601, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87082231, Length=207, Percent_Identity=26.5700483091787, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45471; Mature: 45471

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQITKVIFSSLICNTLLWYDHMLFGHLINIIGGVFFPSDDQLTSVLKAFGVFAVGFLMR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
PVGAAIFGHIGDKHGRRVALLLSIILMTLSTVLIAFVPGYQSIGILASMLVVCLRLLQGI
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
SLGGEAGNAPFLIEHAPKNKKGFFGSIEVLSAIFGSVLSLIAVLICKKVSDFESWGWRLP
CCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
FIFSLAMGLISIYMRYILDESPEYKRQKNPSKLPLKELLNNYKKPVLISIGIDLVENASL
HHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHCCCCEEEEECCHHHCCCCH
YIYLVFFNVLTNKILTVNTHFNHIVEILSQIALGGLTILFAILSDKVGREKVMKFAFIAF
HHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIVSYPVFSMLCSGNYLLAHILFIIPIAASLGPVSALMCELFPTKVRYSGFGLSRNIAAG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHH
FFGGLAPFICTTIIKVTGQETSASFYMIFCALIGLVAISQIKGELSSKSPKVNQV
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQQITKVIFSSLICNTLLWYDHMLFGHLINIIGGVFFPSDDQLTSVLKAFGVFAVGFLMR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
PVGAAIFGHIGDKHGRRVALLLSIILMTLSTVLIAFVPGYQSIGILASMLVVCLRLLQGI
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
SLGGEAGNAPFLIEHAPKNKKGFFGSIEVLSAIFGSVLSLIAVLICKKVSDFESWGWRLP
CCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
FIFSLAMGLISIYMRYILDESPEYKRQKNPSKLPLKELLNNYKKPVLISIGIDLVENASL
HHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHCCCCEEEEECCHHHCCCCH
YIYLVFFNVLTNKILTVNTHFNHIVEILSQIALGGLTILFAILSDKVGREKVMKFAFIAF
HHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIVSYPVFSMLCSGNYLLAHILFIIPIAASLGPVSALMCELFPTKVRYSGFGLSRNIAAG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHH
FFGGLAPFICTTIIKVTGQETSASFYMIFCALIGLVAISQIKGELSSKSPKVNQV
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]