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The map label for this gene is pdxJ [H]

Identifier: 225629918

GI number: 225629918

Start: 60655

End: 61362

Strand: Direct

Name: pdxJ [H]

Synonym: WRi_000550

Alternate gene names: 225629918

Gene position: 60655-61362 (Clockwise)

Preceding gene: 225629917

Following gene: 225629922

Centisome position: 4.2

GC content: 36.72

Gene sequence:

>708_bases
GTGAAACTAGGTGTTAATATCGATCACGTTGCAACCCTCCGCAACGCACGCGGAACTTCTTATCCAGATCCATTGAAAGC
AGCAGAAATAGCCATTGACGCTGGAGCAGACTTTATTACTGTACACTTACGAGAAGATAGAAGGCATATTAGAGATGAAG
ACGTATTTAACCTAAAAAAAAGCATTAGCACTGAGTTAAATCTTGAAATTGCAGCCACGGAAGAAATGCTTGAAATAGCA
AAAAAGGTAAAACCCTATTCGATTAGTATAGTACCAGAAAAAAGAGAAGAATTAACAACCGAAGGTGGCCTGGATATCGT
GAATATGCACAGTAAACTTTCTAGTATAATAGAGGAAATGCATAGCTCTAGCATAAAAGTCTCACTGTTTATCGATCCAA
ATATTGATCAACTAAAATATCTTGAAAAGCTAGGGACAAAACCTGACATAATAGAAATTCACACAGGGGATTACTGTGAT
AATCCATCAGAAAAAAAGTTACAGTTAATTACTAATGCTGCAGAGTACGTCAATAAATTAGGAATAGAATGCCACGCAGG
ACATGGCATAACTTATGAACACGCTAAGAAGATGACAAGAATACCTCACATCTCAGCTCTTAACATAGGCCACTATTTAA
TTAGCGAAGCTGTATTTTACGGCTTACACAGCGTAGTAAAAACAATGAAAATGACAATGTCTAACTAA

Upstream 100 bases:

>100_bases
CTCCTTTCTTTCTCTCCACTTTTTCTGGGTAAGGTCAAACAATGACTATGCAAAAAAACTAATAACATGTATAATCTATA
AATAATTTCTGAGGTAGATT

Downstream 100 bases:

>100_bases
CTGCTGCTTTTTCCTTCTCATTTAAAATACTTAAAAGAGTTTGGCTTGCTTTAAATTTTACCCTTGTCTTTTTAGGAACA
GTCATGATCTCACCACTTTG

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MKLGVNIDHVATLRNARGTSYPDPLKAAEIAIDAGADFITVHLREDRRHIRDEDVFNLKKSISTELNLEIAATEEMLEIA
KKVKPYSISIVPEKREELTTEGGLDIVNMHSKLSSIIEEMHSSSIKVSLFIDPNIDQLKYLEKLGTKPDIIEIHTGDYCD
NPSEKKLQLITNAAEYVNKLGIECHAGHGITYEHAKKMTRIPHISALNIGHYLISEAVFYGLHSVVKTMKMTMSN

Sequences:

>Translated_235_residues
MKLGVNIDHVATLRNARGTSYPDPLKAAEIAIDAGADFITVHLREDRRHIRDEDVFNLKKSISTELNLEIAATEEMLEIA
KKVKPYSISIVPEKREELTTEGGLDIVNMHSKLSSIIEEMHSSSIKVSLFIDPNIDQLKYLEKLGTKPDIIEIHTGDYCD
NPSEKKLQLITNAAEYVNKLGIECHAGHGITYEHAKKMTRIPHISALNIGHYLISEAVFYGLHSVVKTMKMTMSN
>Mature_235_residues
MKLGVNIDHVATLRNARGTSYPDPLKAAEIAIDAGADFITVHLREDRRHIRDEDVFNLKKSISTELNLEIAATEEMLEIA
KKVKPYSISIVPEKREELTTEGGLDIVNMHSKLSSIIEEMHSSSIKVSLFIDPNIDQLKYLEKLGTKPDIIEIHTGDYCD
NPSEKKLQLITNAAEYVNKLGIECHAGHGITYEHAKKMTRIPHISALNIGHYLISEAVFYGLHSVVKTMKMTMSN

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate [H]

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788917, Length=235, Percent_Identity=50.6382978723404, Blast_Score=236, Evalue=1e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004569 [H]

Pfam domain/function: PF03740 PdxJ [H]

EC number: =2.6.99.2 [H]

Molecular weight: Translated: 26349; Mature: 26349

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLGVNIDHVATLRNARGTSYPDPLKAAEIAIDAGADFITVHLREDRRHIRDEDVFNLKK
CCCCCCHHHHHHHHHCCCCCCCCCCCHHHEEEECCCCEEEEEEEHHHHHCCCCHHHHHHH
SISTELNLEIAATEEMLEIAKKVKPYSISIVPEKREELTTEGGLDIVNMHSKLSSIIEEM
HCCCCEEEEEECHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCEEEHHHHHHHHHHHHH
HSSSIKVSLFIDPNIDQLKYLEKLGTKPDIIEIHTGDYCDNPSEKKLQLITNAAEYVNKL
CCCCEEEEEEECCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHC
GIECHAGHGITYEHAKKMTRIPHISALNIGHYLISEAVFYGLHSVVKTMKMTMSN
CCEEECCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLGVNIDHVATLRNARGTSYPDPLKAAEIAIDAGADFITVHLREDRRHIRDEDVFNLKK
CCCCCCHHHHHHHHHCCCCCCCCCCCHHHEEEECCCCEEEEEEEHHHHHCCCCHHHHHHH
SISTELNLEIAATEEMLEIAKKVKPYSISIVPEKREELTTEGGLDIVNMHSKLSSIIEEM
HCCCCEEEEEECHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCEEEHHHHHHHHHHHHH
HSSSIKVSLFIDPNIDQLKYLEKLGTKPDIIEIHTGDYCDNPSEKKLQLITNAAEYVNKL
CCCCEEEEEEECCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHC
GIECHAGHGITYEHAKKMTRIPHISALNIGHYLISEAVFYGLHSVVKTMKMTMSN
CCEEECCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA