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The map label for this gene is dnaA

Identifier: 225629873

GI number: 225629873

Start: 153

End: 1535

Strand: Direct

Name: dnaA

Synonym: WRi_000010

Alternate gene names: 225629873

Gene position: 153-1535 (Clockwise)

Preceding gene: NA

Following gene: 225629875

Centisome position: 0.01

GC content: 34.49

Gene sequence:

>1383_bases
ATGAACTTAACTAGCCCTAAGGTTTCTACTATGTTTTTTGATCAGATTATTACAGTAACGGATCACAATGTTACTTGGGA
AAAAATCCAAAATTGTCTTTATAATCTTTATGGAGAAGCAACATATAATAGCTGGCTGAGTTCACTAAAATTTGTCAGTA
GCAGAAATGGGGAAGTTCTTTTATCCGTGTCAACAAGGTTTATAAAAGAGTGGATTACAGTTCATTACATGAAAAAAATA
TTATCATTATGGCAAAGCGAAGATAAAAGTATACGTTCTATTGATATTCAAGTAATTGAGGAGAGGAATTCAAATTTTAA
TGTTATACTAAAAAACAGAGAGGAAAGTAATCATAATCTTGGTTCGCCGCTGGATCCAAGGTTCACCTTTGATAATTTTG
TAGTGGGAAAGCCAAATGAATTAGCATTTACGGCGGCAAAGCGTGTGGCAGAATCTATAGATCCAATATTAGGCAGCAAT
CCTTTGTTTCTATATGGTGGAGTGGGACTTGGTAAAACACATCTAATGCATGCTATAGCTTGGCACATAGTCAATTCCCC
ATCAGCAAAAAGAAAAGTGGTGTATTTATCAGCAGAGAAATTCATGTACCAATATATTACAGCGCTGCGAAGCAAAGATA
TTATGTTATTTAAAGAGCAATTTAGATCAGTAGATGTATTGATGGTAGATGATGTGCAATTTATCAGTGGTAAAGATAGT
ACACAAGAAGAATTTTTTCACACTTTCAATGCATTGATAGACCAAAATAAACAATTGGTTATATCAGCTGATAGGTCTCC
TAGTGATCTTGATGGAGTGGAAGAAAGAATAAAATCACGACTCGGTTGGGGGTTGGTGGCAGATATTAATGAAACAACTT
TTGAATTAAGGCTTGGTATATTGCAGGCAAAAGTGGAGCAGATGAATATGTATGTTCCGAAAGATGTCCTAGAGTTTTTA
GCAAGGAACATAAAATCTAATATAAGAGAATTAGAAGGAGCATTAAATAAGGTTACCCATACCTCATTAATTGGAAGAAG
TATGACGGTAGAATCAGCTAGTGAAACCCTAATCGATCTTCTTAGGTCAAATCATAGGTCAGTCACAATAGAAGAAATAC
AAAAGAAAGTAGCTGAATTTTTCAATATAAAGGTTGCAGATATGCAATCCAATAGAAGGCTTCGCAGTCTTGCAAGGCCA
AGACAAATAGCTATGTATTTTGCCAAAAAGTTTACGCAAAAAAGCCTACCAGATATTGGGAGAAACTTTGGTGGCAGAGA
CCATGCTACTGTTATACATGCAGTAAAACAAGTAGAAAATTTTATAAAGACCGACTCAAAATTTGCTGATGAAATCAATC
GATTAAAAAAAATGTTTAAGTAG

Upstream 100 bases:

>100_bases
AATTTTTAGTATGTATTAAGTAGTATTAGCTTTTCATTTTGCAGTAAGCTATTGATTATCTTATATTTTTCTAATTATTG
CTTTTTTCTTGGTGTGGTAT

Downstream 100 bases:

>100_bases
CGTTTTTAAAATAGCTAATAACAAATCCTAAAATGAATCGACTATAGTTTTAATCCGTTTAAGTTTAAAATCTTCTGTTA
GCTTCGCAGAATACATTTCC

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MNLTSPKVSTMFFDQIITVTDHNVTWEKIQNCLYNLYGEATYNSWLSSLKFVSSRNGEVLLSVSTRFIKEWITVHYMKKI
LSLWQSEDKSIRSIDIQVIEERNSNFNVILKNREESNHNLGSPLDPRFTFDNFVVGKPNELAFTAAKRVAESIDPILGSN
PLFLYGGVGLGKTHLMHAIAWHIVNSPSAKRKVVYLSAEKFMYQYITALRSKDIMLFKEQFRSVDVLMVDDVQFISGKDS
TQEEFFHTFNALIDQNKQLVISADRSPSDLDGVEERIKSRLGWGLVADINETTFELRLGILQAKVEQMNMYVPKDVLEFL
ARNIKSNIRELEGALNKVTHTSLIGRSMTVESASETLIDLLRSNHRSVTIEEIQKKVAEFFNIKVADMQSNRRLRSLARP
RQIAMYFAKKFTQKSLPDIGRNFGGRDHATVIHAVKQVENFIKTDSKFADEINRLKKMFK

Sequences:

>Translated_460_residues
MNLTSPKVSTMFFDQIITVTDHNVTWEKIQNCLYNLYGEATYNSWLSSLKFVSSRNGEVLLSVSTRFIKEWITVHYMKKI
LSLWQSEDKSIRSIDIQVIEERNSNFNVILKNREESNHNLGSPLDPRFTFDNFVVGKPNELAFTAAKRVAESIDPILGSN
PLFLYGGVGLGKTHLMHAIAWHIVNSPSAKRKVVYLSAEKFMYQYITALRSKDIMLFKEQFRSVDVLMVDDVQFISGKDS
TQEEFFHTFNALIDQNKQLVISADRSPSDLDGVEERIKSRLGWGLVADINETTFELRLGILQAKVEQMNMYVPKDVLEFL
ARNIKSNIRELEGALNKVTHTSLIGRSMTVESASETLIDLLRSNHRSVTIEEIQKKVAEFFNIKVADMQSNRRLRSLARP
RQIAMYFAKKFTQKSLPDIGRNFGGRDHATVIHAVKQVENFIKTDSKFADEINRLKKMFK
>Mature_460_residues
MNLTSPKVSTMFFDQIITVTDHNVTWEKIQNCLYNLYGEATYNSWLSSLKFVSSRNGEVLLSVSTRFIKEWITVHYMKKI
LSLWQSEDKSIRSIDIQVIEERNSNFNVILKNREESNHNLGSPLDPRFTFDNFVVGKPNELAFTAAKRVAESIDPILGSN
PLFLYGGVGLGKTHLMHAIAWHIVNSPSAKRKVVYLSAEKFMYQYITALRSKDIMLFKEQFRSVDVLMVDDVQFISGKDS
TQEEFFHTFNALIDQNKQLVISADRSPSDLDGVEERIKSRLGWGLVADINETTFELRLGILQAKVEQMNMYVPKDVLEFL
ARNIKSNIRELEGALNKVTHTSLIGRSMTVESASETLIDLLRSNHRSVTIEEIQKKVAEFFNIKVADMQSNRRLRSLARP
RQIAMYFAKKFTQKSLPDIGRNFGGRDHATVIHAVKQVENFIKTDSKFADEINRLKKMFK

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family

Homologues:

Organism=Escherichia coli, GI2367267, Length=446, Percent_Identity=40.1345291479821, Blast_Score=328, Evalue=3e-91,
Organism=Escherichia coli, GI226510964, Length=201, Percent_Identity=23.3830845771144, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAA_WOLPM (Q73IZ0)

Other databases:

- EMBL:   AE017196
- RefSeq:   NP_965836.1
- ProteinModelPortal:   Q73IZ0
- SMR:   Q73IZ0
- EnsemblBacteria:   EBWOLT00000004306
- GeneID:   2738730
- GenomeReviews:   AE017196_GR
- KEGG:   wol:WD0001
- TIGR:   WD_0001
- GeneTree:   EBGT00050000030965
- HOGENOM:   HBG695666
- OMA:   CHTLNAL
- PhylomeDB:   Q73IZ0
- ProtClustDB:   PRK00149
- BioCyc:   WPIP955:WD_0001-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00377
- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921
- Gene3D:   G3DSA:1.10.1750.10
- PRINTS:   PR00051
- SMART:   SM00382
- SMART:   SM00760
- TIGRFAMs:   TIGR00362

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C; SSF48295 Trp_repress_rep

EC number: NA

Molecular weight: Translated: 52807; Mature: 52807

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLTSPKVSTMFFDQIITVTDHNVTWEKIQNCLYNLYGEATYNSWLSSLKFVSSRNGEVL
CCCCCCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEE
LSVSTRFIKEWITVHYMKKILSLWQSEDKSIRSIDIQVIEERNSNFNVILKNREESNHNL
EEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEHEEEECCCCCEEEEEECCCCCCCCC
GSPLDPRFTFDNFVVGKPNELAFTAAKRVAESIDPILGSNPLFLYGGVGLGKTHLMHAIA
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHH
WHIVNSPSAKRKVVYLSAEKFMYQYITALRSKDIMLFKEQFRSVDVLMVDDVQFISGKDS
HHHCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCHHHCCCCC
TQEEFFHTFNALIDQNKQLVISADRSPSDLDGVEERIKSRLGWGLVADINETTFELRLGI
HHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEECCCCHHHHHHHHH
LQAKVEQMNMYVPKDVLEFLARNIKSNIRELEGALNKVTHTSLIGRSMTVESASETLIDL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LRSNHRSVTIEEIQKKVAEFFNIKVADMQSNRRLRSLARPRQIAMYFAKKFTQKSLPDIG
HHCCCCEEEHHHHHHHHHHHHCCEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCHHH
RNFGGRDHATVIHAVKQVENFIKTDSKFADEINRLKKMFK
HCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLTSPKVSTMFFDQIITVTDHNVTWEKIQNCLYNLYGEATYNSWLSSLKFVSSRNGEVL
CCCCCCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEE
LSVSTRFIKEWITVHYMKKILSLWQSEDKSIRSIDIQVIEERNSNFNVILKNREESNHNL
EEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEHEEEECCCCCEEEEEECCCCCCCCC
GSPLDPRFTFDNFVVGKPNELAFTAAKRVAESIDPILGSNPLFLYGGVGLGKTHLMHAIA
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHH
WHIVNSPSAKRKVVYLSAEKFMYQYITALRSKDIMLFKEQFRSVDVLMVDDVQFISGKDS
HHHCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCHHHCCCCC
TQEEFFHTFNALIDQNKQLVISADRSPSDLDGVEERIKSRLGWGLVADINETTFELRLGI
HHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEECCCCHHHHHHHHH
LQAKVEQMNMYVPKDVLEFLARNIKSNIRELEGALNKVTHTSLIGRSMTVESASETLIDL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LRSNHRSVTIEEIQKKVAEFFNIKVADMQSNRRLRSLARPRQIAMYFAKKFTQKSLPDIG
HHCCCCEEEHHHHHHHHHHHHCCEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCHHH
RNFGGRDHATVIHAVKQVENFIKTDSKFADEINRLKKMFK
HCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA