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The map label for this gene is braE [H]

Identifier: 222527143

GI number: 222527143

Start: 4873977

End: 4875020

Strand: Direct

Name: braE [H]

Synonym: Chy400_3925

Alternate gene names: 222527143

Gene position: 4873977-4875020 (Clockwise)

Preceding gene: 222527142

Following gene: 222527144

Centisome position: 92.5

GC content: 58.24

Gene sequence:

>1044_bases
ATGCTGACACGTTTGAAAGCACCACAATGGGCCGGTCTGGCCTTGATCGGCGGTCTGATCGCCTTCCCCTATCTGATAGC
ACTGCTCACCGGGCAGCCAATTGACAGTGGGATACCGAAATTCTGGCAGGGTATGCTCATCCAGGTCTTCATTCTGGCCG
TTTTTGCGATGAGCTACGACATTCTGATGGGCTATACCGGCATTCTCTCCTTCGGCCACGCCCTCTTCTTCGGCACAGGT
GCCTACACGATTGGGATTTTGCTCAAACATGCCGGCTGGGATCTCGGCTCAGCATTCGTGGCCGTCATCGTCATTGCGGT
ACTGCAAAGCCTGATCGTTGGCCTGCTCTCGTTGCGGGTCAGCGGTGTCTACTTCGCGATGGTGACACTGGCCTTTGCCC
AGATGTTCTTCCTCCTGGCCGAAGCAACCGACTTTCGCCAGTGGACAGGGGCAGAGGATGGGTTGCAAAGTATTCCGGTA
CCGGCGTGGATCAGCCCGACCAACGAACGTCTGCGCTTCTACTATCTTGCGTTAGGATTTGCAGTAGTGATGTATCTCCT
GGCCCGCCAGGTCGTCAATTCGCCAACCGGGCGGGTGATGATGGCGATCCGCGACAACGAGGTACGGGCCAGAGTCTTGG
GCTACCATACGCTCACCTACAAGCTGATCGCCATCACCATCAGCGGTGTTATGGCAGCGCTGGCCGGTGCATTCAACGCA
CTGTGGAATCTCAACGCAAACCCGGCGGTGCTCAGCGTAGGGACAACGATCAATGCGCTCCTGATGACGATCATTGGTGG
CGTCGGTTCGCTGATCGGGCCGATGCTTGGCGCCGCCGTGATCCAACTGCTGGGGTACTGGCTGAACTTCTTCTTCGGGC
CGGCATGGCCACTGCTGTTTGGGATCGTGTACATTCTCATCGTGCTCTTCCTGCCATACGGCATCGTCGGTACCTGGCGG
TTGCGCGGTACGAGTTGGGTGACGATGTGGCGCAACCGCTGGCAGGAATTAGCCAGGCGACGCGGTGACGGCGTAGCGCG
GTAG

Upstream 100 bases:

>100_bases
CACCTTCTCACCTTCAATTGCGCGCGCTTCAACAGTCTTGATCATGGCGCTGGTGCTCTTGCTGCGCCCGGCAGGTCTCT
TTGGGAAGAAGGAGTAGGCG

Downstream 100 bases:

>100_bases
GGTGGTGATACTTCATAAGAGCGGGGTGGATGTCCACCTCGCTTTTGCGTGTATTGCAGGTGATGTTTTGCTGCGGAGGG
GGATGTGCATTCAAGACTGG

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MLTRLKAPQWAGLALIGGLIAFPYLIALLTGQPIDSGIPKFWQGMLIQVFILAVFAMSYDILMGYTGILSFGHALFFGTG
AYTIGILLKHAGWDLGSAFVAVIVIAVLQSLIVGLLSLRVSGVYFAMVTLAFAQMFFLLAEATDFRQWTGAEDGLQSIPV
PAWISPTNERLRFYYLALGFAVVMYLLARQVVNSPTGRVMMAIRDNEVRARVLGYHTLTYKLIAITISGVMAALAGAFNA
LWNLNANPAVLSVGTTINALLMTIIGGVGSLIGPMLGAAVIQLLGYWLNFFFGPAWPLLFGIVYILIVLFLPYGIVGTWR
LRGTSWVTMWRNRWQELARRRGDGVAR

Sequences:

>Translated_347_residues
MLTRLKAPQWAGLALIGGLIAFPYLIALLTGQPIDSGIPKFWQGMLIQVFILAVFAMSYDILMGYTGILSFGHALFFGTG
AYTIGILLKHAGWDLGSAFVAVIVIAVLQSLIVGLLSLRVSGVYFAMVTLAFAQMFFLLAEATDFRQWTGAEDGLQSIPV
PAWISPTNERLRFYYLALGFAVVMYLLARQVVNSPTGRVMMAIRDNEVRARVLGYHTLTYKLIAITISGVMAALAGAFNA
LWNLNANPAVLSVGTTINALLMTIIGGVGSLIGPMLGAAVIQLLGYWLNFFFGPAWPLLFGIVYILIVLFLPYGIVGTWR
LRGTSWVTMWRNRWQELARRRGDGVAR
>Mature_347_residues
MLTRLKAPQWAGLALIGGLIAFPYLIALLTGQPIDSGIPKFWQGMLIQVFILAVFAMSYDILMGYTGILSFGHALFFGTG
AYTIGILLKHAGWDLGSAFVAVIVIAVLQSLIVGLLSLRVSGVYFAMVTLAFAQMFFLLAEATDFRQWTGAEDGLQSIPV
PAWISPTNERLRFYYLALGFAVVMYLLARQVVNSPTGRVMMAIRDNEVRARVLGYHTLTYKLIAITISGVMAALAGAFNA
LWNLNANPAVLSVGTTINALLMTIIGGVGSLIGPMLGAAVIQLLGYWLNFFFGPAWPLLFGIVYILIVLFLPYGIVGTWR
LRGTSWVTMWRNRWQELARRRGDGVAR

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=295, Percent_Identity=24.7457627118644, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 38022; Mature: 38022

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRLKAPQWAGLALIGGLIAFPYLIALLTGQPIDSGIPKFWQGMLIQVFILAVFAMSYD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ILMGYTGILSFGHALFFGTGAYTIGILLKHAGWDLGSAFVAVIVIAVLQSLIVGLLSLRV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SGVYFAMVTLAFAQMFFLLAEATDFRQWTGAEDGLQSIPVPAWISPTNERLRFYYLALGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHH
AVVMYLLARQVVNSPTGRVMMAIRDNEVRARVLGYHTLTYKLIAITISGVMAALAGAFNA
HHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWNLNANPAVLSVGTTINALLMTIIGGVGSLIGPMLGAAVIQLLGYWLNFFFGPAWPLLF
HHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
GIVYILIVLFLPYGIVGTWRLRGTSWVTMWRNRWQELARRRGDGVAR
HHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLTRLKAPQWAGLALIGGLIAFPYLIALLTGQPIDSGIPKFWQGMLIQVFILAVFAMSYD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ILMGYTGILSFGHALFFGTGAYTIGILLKHAGWDLGSAFVAVIVIAVLQSLIVGLLSLRV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SGVYFAMVTLAFAQMFFLLAEATDFRQWTGAEDGLQSIPVPAWISPTNERLRFYYLALGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHH
AVVMYLLARQVVNSPTGRVMMAIRDNEVRARVLGYHTLTYKLIAITISGVMAALAGAFNA
HHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWNLNANPAVLSVGTTINALLMTIIGGVGSLIGPMLGAAVIQLLGYWLNFFFGPAWPLLF
HHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
GIVYILIVLFLPYGIVGTWRLRGTSWVTMWRNRWQELARRRGDGVAR
HHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]