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The map label for this gene is amy [H]

Identifier: 222527025

GI number: 222527025

Start: 4735575

End: 4737368

Strand: Reverse

Name: amy [H]

Synonym: Chy400_3804

Alternate gene names: 222527025

Gene position: 4737368-4735575 (Counterclockwise)

Preceding gene: 222527027

Following gene: 222527022

Centisome position: 89.91

GC content: 52.51

Gene sequence:

>1794_bases
ATGCCCGGCACTCGCTTTCCCTCGCTTCGTCGGCTCGTCCTCGTTGTCGCCCTTCTCATGGTGGTAAGTAGTCTTCCGTT
CGGTCCGGTGCACCATTCAACCGCACGTGCCCAAACCTCATCACCACGTACCGTATTTGTTCATCTCTTTGAATGGAAGT
GGACGGACATTGCCCAGGAATGCGAGAACTTTCTGGGGCCACGCGGCTTTGCGGCAGTGCAGGTGTCGCCACCGCAAGAG
CACGCGATTGTTGCCGGTTATCCGTGGTGGCAACGGTATCAACCGGTCAGTTATCAATTGACCAGTCGTAGCGGGACACG
GGCTGAATTCGCCAATATGGTTGCCCGTTGCAAAGCGGTCGGTGTTGACATTTATGTTGATGCGGTCATCAATCATATGA
CCGGCGTCGGCAGCGGTGTCGGATCGGCTGGCTCAACGTATAGCCCGTACAACTATCCGGGCATCTATCAATATCAGGAT
TTTCACCACTGCGGCAGAAATGGCAACGATGACATCCAGAATTATGGTGATCGGTACGAAGTTCAGAACTGCGAACTGGT
GAATCTTGCCGATCTCGATACCGGATCATCGTATGTGCGGGATCGCTTAGCTGCCTATTTGAACGATCTCATCAGTCTGG
GAGTTGCCGGTTTTCGGATTGACGCAGCTAAACACATTGCTGCCGGGGATATTGCCGCAATTTTATCCCGTGTGAATGGG
AGTCCGTACATTTACCAGGAAGTGATCGGTGCGGCTGGCGAACCGATTACACCGTGGGAATACACAAATAATGGTGATGT
CACTGAATTTAAGTATAGCAACGAGATCGGGCGGGTCTTTTTGAATGGTAAGCTGGCATGGCTGAGTCAGTTTGGCGAAG
CCTGGGGGATGCTGCCAAGCGACAAAGCGATTGTCTTCGTTGATAATCACGACAACCAGCGCGGGCATGGCGGTGGTGGG
ACTGTGGTCACATACAAGAATGGTGTGCTGTACGATCTGGCAAACGTGTTTATGCTAGCGTGGCCGTATGGGTACCCCCA
GGTGATGTCAAGTTATGAGTTTAGCAATGATTTTCAAGGGCCACCGAGTGATGCGAACGGCAACACGCGCAGCGTCTATG
TTAACGGCCAGCCCAATTGCTTTGGCGAATGGAAATGCGAGCATCGCTGGCGACCAATTGCGAATATGGTAGCGTTCCGC
AATGCCACAGCGAGTACATTCAGTGTGAGTGATTGGTGGAGTAACGGCAACAACCAGATCGCCTTTGGTCGTGGCGATAA
AGGGTTTGTCGTTATCAATCGTGAGGATACAACGCTGAATCGCACGTTTCAGACGAGTATGGCGCCTGGGGTCTACTGCA
ATGTGATTGTTGCCGATTTTACAAACGGTACGTGCAGTGGGCAAACCGTCACCGTGGACAGTAATCGACGGATAACGGTC
TCTATTCCGCCTTTCAGTGCTCTTGCCATCCATGTAGGAGCGAAGTTGTCTACGCAACCGGCAACTGTTGCGGTTACTTT
CAACGTGAATGCGACGACCTACTGGGGGCAGAACGTGTTTGTGGTTGGGAATATCCCGCAATTGGGCAACTGGAACCCGG
CGCAGGCTGTGCCCCTTTCAGCGGCTACGTATCCGGTCTGGAGTGGTACCGTTAATCTGCCGGCAAATACCACCATCGAA
TACAAGTACATTAAGCGTGACGGATCAAATGTGGTGTGGGAGTGTTGTAATAATCGCGTTATTACGACGCCAGGTAGTGG
CTCGATGACGCTGAATGAGACGTGGCGTCCGTGA

Upstream 100 bases:

>100_bases
AACAAATGCAAGAAAAACAGTTCGGTAAGCATCATTCCACTACTCACTGTTGTTGAGTCTGGTCAGCGTTGGCCGCTTCC
TGGAGCAAAGGAGCCTGTTT

Downstream 100 bases:

>100_bases
GGTGATGCCAGTATTCGCCGAGAGAGGGGCAAGTATCGCTCCTCTCTCGGTTTATTTTGCCGTTATGTCGCCGCTTACTC
CATGCGGTGCATCGTTTGGG

Product: alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 597; Mature: 596

Protein sequence:

>597_residues
MPGTRFPSLRRLVLVVALLMVVSSLPFGPVHHSTARAQTSSPRTVFVHLFEWKWTDIAQECENFLGPRGFAAVQVSPPQE
HAIVAGYPWWQRYQPVSYQLTSRSGTRAEFANMVARCKAVGVDIYVDAVINHMTGVGSGVGSAGSTYSPYNYPGIYQYQD
FHHCGRNGNDDIQNYGDRYEVQNCELVNLADLDTGSSYVRDRLAAYLNDLISLGVAGFRIDAAKHIAAGDIAAILSRVNG
SPYIYQEVIGAAGEPITPWEYTNNGDVTEFKYSNEIGRVFLNGKLAWLSQFGEAWGMLPSDKAIVFVDNHDNQRGHGGGG
TVVTYKNGVLYDLANVFMLAWPYGYPQVMSSYEFSNDFQGPPSDANGNTRSVYVNGQPNCFGEWKCEHRWRPIANMVAFR
NATASTFSVSDWWSNGNNQIAFGRGDKGFVVINREDTTLNRTFQTSMAPGVYCNVIVADFTNGTCSGQTVTVDSNRRITV
SIPPFSALAIHVGAKLSTQPATVAVTFNVNATTYWGQNVFVVGNIPQLGNWNPAQAVPLSAATYPVWSGTVNLPANTTIE
YKYIKRDGSNVVWECCNNRVITTPGSGSMTLNETWRP

Sequences:

>Translated_597_residues
MPGTRFPSLRRLVLVVALLMVVSSLPFGPVHHSTARAQTSSPRTVFVHLFEWKWTDIAQECENFLGPRGFAAVQVSPPQE
HAIVAGYPWWQRYQPVSYQLTSRSGTRAEFANMVARCKAVGVDIYVDAVINHMTGVGSGVGSAGSTYSPYNYPGIYQYQD
FHHCGRNGNDDIQNYGDRYEVQNCELVNLADLDTGSSYVRDRLAAYLNDLISLGVAGFRIDAAKHIAAGDIAAILSRVNG
SPYIYQEVIGAAGEPITPWEYTNNGDVTEFKYSNEIGRVFLNGKLAWLSQFGEAWGMLPSDKAIVFVDNHDNQRGHGGGG
TVVTYKNGVLYDLANVFMLAWPYGYPQVMSSYEFSNDFQGPPSDANGNTRSVYVNGQPNCFGEWKCEHRWRPIANMVAFR
NATASTFSVSDWWSNGNNQIAFGRGDKGFVVINREDTTLNRTFQTSMAPGVYCNVIVADFTNGTCSGQTVTVDSNRRITV
SIPPFSALAIHVGAKLSTQPATVAVTFNVNATTYWGQNVFVVGNIPQLGNWNPAQAVPLSAATYPVWSGTVNLPANTTIE
YKYIKRDGSNVVWECCNNRVITTPGSGSMTLNETWRP
>Mature_596_residues
PGTRFPSLRRLVLVVALLMVVSSLPFGPVHHSTARAQTSSPRTVFVHLFEWKWTDIAQECENFLGPRGFAAVQVSPPQEH
AIVAGYPWWQRYQPVSYQLTSRSGTRAEFANMVARCKAVGVDIYVDAVINHMTGVGSGVGSAGSTYSPYNYPGIYQYQDF
HHCGRNGNDDIQNYGDRYEVQNCELVNLADLDTGSSYVRDRLAAYLNDLISLGVAGFRIDAAKHIAAGDIAAILSRVNGS
PYIYQEVIGAAGEPITPWEYTNNGDVTEFKYSNEIGRVFLNGKLAWLSQFGEAWGMLPSDKAIVFVDNHDNQRGHGGGGT
VVTYKNGVLYDLANVFMLAWPYGYPQVMSSYEFSNDFQGPPSDANGNTRSVYVNGQPNCFGEWKCEHRWRPIANMVAFRN
ATASTFSVSDWWSNGNNQIAFGRGDKGFVVINREDTTLNRTFQTSMAPGVYCNVIVADFTNGTCSGQTVTVDSNRRITVS
IPPFSALAIHVGAKLSTQPATVAVTFNVNATTYWGQNVFVVGNIPQLGNWNPAQAVPLSAATYPVWSGTVNLPANTTIEY
KYIKRDGSNVVWECCNNRVITTPGSGSMTLNETWRP

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI56549664, Length=519, Percent_Identity=46.6281310211946, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI56549662, Length=519, Percent_Identity=46.6281310211946, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI40254482, Length=519, Percent_Identity=46.6281310211946, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI56549660, Length=519, Percent_Identity=46.6281310211946, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI10280622, Length=496, Percent_Identity=47.5806451612903, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI4502085, Length=496, Percent_Identity=47.1774193548387, Blast_Score=431, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17558728, Length=563, Percent_Identity=39.2539964476021, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI18859631, Length=507, Percent_Identity=48.1262327416174, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24654362, Length=476, Percent_Identity=50, Blast_Score=443, Evalue=1e-124,
Organism=Drosophila melanogaster, GI17137384, Length=475, Percent_Identity=47.7894736842105, Blast_Score=430, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR006046
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 65644; Mature: 65513

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS51166 CBM20

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGTRFPSLRRLVLVVALLMVVSSLPFGPVHHSTARAQTSSPRTVFVHLFEWKWTDIAQE
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHH
CENFLGPRGFAAVQVSPPQEHAIVAGYPWWQRYQPVSYQLTSRSGTRAEFANMVARCKAV
HHHHCCCCCEEEEEECCCCCCEEEECCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHC
GVDIYVDAVINHMTGVGSGVGSAGSTYSPYNYPGIYQYQDFHHCGRNGNDDIQNYGDRYE
CEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECHHHHHCCCCCCHHHHCCCCEE
VQNCELVNLADLDTGSSYVRDRLAAYLNDLISLGVAGFRIDAAKHIAAGDIAAILSRVNG
ECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCC
SPYIYQEVIGAAGEPITPWEYTNNGDVTEFKYSNEIGRVFLNGKLAWLSQFGEAWGMLPS
CCCHHHHHHCCCCCCCCCCEECCCCCEEEEEECCCCEEEEECCEEHHHHHHHHHHCCCCC
DKAIVFVDNHDNQRGHGGGGTVVTYKNGVLYDLANVFMLAWPYGYPQVMSSYEFSNDFQG
CCEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHCCCEEECCCCCHHHHHCCCCCCCCCC
PPSDANGNTRSVYVNGQPNCFGEWKCEHRWRPIANMVAFRNATASTFSVSDWWSNGNNQI
CCCCCCCCEEEEEECCCCCCCCCCEECCCCCCHHHHHHHCCCCCCEEEEHHHHCCCCCEE
AFGRGDKGFVVINREDTTLNRTFQTSMAPGVYCNVIVADFTNGTCSGQTVTVDSNRRITV
EECCCCCCEEEEECCCCCCCEEEECCCCCCEEEEEEEEECCCCCCCCCEEEECCCCEEEE
SIPPFSALAIHVGAKLSTQPATVAVTFNVNATTYWGQNVFVVGNIPQLGNWNPAQAVPLS
EECCCCEEEEEECCEECCCCEEEEEEEEECEEEEECCEEEEEECCCCCCCCCCCCCCCCC
AATYPVWSGTVNLPANTTIEYKYIKRDGSNVVWECCNNRVITTPGSGSMTLNETWRP
CCCCEEECCEEECCCCCEEEEEEEEECCCCHHHHHCCCCEEECCCCCCEEECCCCCC
>Mature Secondary Structure 
PGTRFPSLRRLVLVVALLMVVSSLPFGPVHHSTARAQTSSPRTVFVHLFEWKWTDIAQE
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHH
CENFLGPRGFAAVQVSPPQEHAIVAGYPWWQRYQPVSYQLTSRSGTRAEFANMVARCKAV
HHHHCCCCCEEEEEECCCCCCEEEECCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHC
GVDIYVDAVINHMTGVGSGVGSAGSTYSPYNYPGIYQYQDFHHCGRNGNDDIQNYGDRYE
CEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECHHHHHCCCCCCHHHHCCCCEE
VQNCELVNLADLDTGSSYVRDRLAAYLNDLISLGVAGFRIDAAKHIAAGDIAAILSRVNG
ECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCC
SPYIYQEVIGAAGEPITPWEYTNNGDVTEFKYSNEIGRVFLNGKLAWLSQFGEAWGMLPS
CCCHHHHHHCCCCCCCCCCEECCCCCEEEEEECCCCEEEEECCEEHHHHHHHHHHCCCCC
DKAIVFVDNHDNQRGHGGGGTVVTYKNGVLYDLANVFMLAWPYGYPQVMSSYEFSNDFQG
CCEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHCCCEEECCCCCHHHHHCCCCCCCCCC
PPSDANGNTRSVYVNGQPNCFGEWKCEHRWRPIANMVAFRNATASTFSVSDWWSNGNNQI
CCCCCCCCEEEEEECCCCCCCCCCEECCCCCCHHHHHHHCCCCCCEEEEHHHHCCCCCEE
AFGRGDKGFVVINREDTTLNRTFQTSMAPGVYCNVIVADFTNGTCSGQTVTVDSNRRITV
EECCCCCCEEEEECCCCCCCEEEECCCCCCEEEEEEEEECCCCCCCCCEEEECCCCEEEE
SIPPFSALAIHVGAKLSTQPATVAVTFNVNATTYWGQNVFVVGNIPQLGNWNPAQAVPLS
EECCCCEEEEEECCEECCCCEEEEEEEEECEEEEECCEEEEEECCCCCCCCCCCCCCCCC
AATYPVWSGTVNLPANTTIEYKYIKRDGSNVVWECCNNRVITTPGSGSMTLNETWRP
CCCCEEECCEEECCCCCEEEEEEEEECCCCHHHHHCCCCEEECCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1544904; 9575155; 9541387 [H]