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The map label for this gene is gph [C]

Identifier: 222526278

GI number: 222526278

Start: 3754299

End: 3755030

Strand: Reverse

Name: gph [C]

Synonym: Chy400_3044

Alternate gene names: 222526278

Gene position: 3755030-3754299 (Counterclockwise)

Preceding gene: 222526281

Following gene: 222526274

Centisome position: 71.27

GC content: 59.29

Gene sequence:

>732_bases
ATGGGCATGAAGAGACAACACCGTTTTCGCGCAATCCTGTTCGATCTTGACGGCACACTGACCGATCCCTTCACCGGTAT
TGCCCGCAGCGTCAATTATGCCCTGCAACGGCTAGGGCATCCACTCCTCAGCGACGACGAGCTTCGGCGCTGGATTGGGC
CTCCCTTGCACGCCTCGTTCGAGCAACTGCTTGACGACCCACGGTTAGCCGATCAGGCGGTGGCTCACTATCGGCAACGC
TATTGGGAAACCGGCCTGTACGAGAACACCGTCTTCCCTGGCATTCCCGAACTCCTGTCTGACCTCAAAGCCGCAGGCAT
CCGGCTCTTCGTCGCCACCTCGAAAATTCGTCCGCCTACCGAACGGATTCTGGCCCACTTTGGGCTGCAACCCTTCTTCG
AGGCAATCGCTGCCGTCGATCCCGATGACCGCGATGGCAACAAGGCAATGGTCATCGGCACCCTCTTACCAACGCTCGGT
GCTGATCGCGCCTGGACGGTGATGGTGGGGGATACGATCTTCGACATCCACGGCGCACGTGCCCATCGGATGCCCTGTAT
TGCGGTGACCTATGGATATGGGGAGTACCGTGATCTCTACCTTGCCGCCCCGCTCGCCCTGGCTAGCACGGTGGGTACAT
TGCGAGAGTTATTGAGCGCGGATGATCCACATTCGGTCAGATACCGTCATTCACGGCCCGGCACGGGAAGCCAACATGAT
CCTACAGCGTAG

Upstream 100 bases:

>100_bases
CAGGCGTCTCAGATGCATATCTCGGTTATTCAGACCGGATGAGAGTATTATAACACCGTTTCCCGGCGTTTGCCGGGTAG
CACGGTTTTTGCGTTATACT

Downstream 100 bases:

>100_bases
CGAAGTGAGTTTGTGCGAAGAGTATTGGAAGAGACCAGAACAGAGATCATGGTAGGCTGAACATCGACATCAGACGTAGT
CGAAGCGGGACATGAACATC

Product: HAD-superfamily hydrolase

Products: NA

Alternate protein names: Nucleoside 5'-monophosphate phosphohydrolase [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MGMKRQHRFRAILFDLDGTLTDPFTGIARSVNYALQRLGHPLLSDDELRRWIGPPLHASFEQLLDDPRLADQAVAHYRQR
YWETGLYENTVFPGIPELLSDLKAAGIRLFVATSKIRPPTERILAHFGLQPFFEAIAAVDPDDRDGNKAMVIGTLLPTLG
ADRAWTVMVGDTIFDIHGARAHRMPCIAVTYGYGEYRDLYLAAPLALASTVGTLRELLSADDPHSVRYRHSRPGTGSQHD
PTA

Sequences:

>Translated_243_residues
MGMKRQHRFRAILFDLDGTLTDPFTGIARSVNYALQRLGHPLLSDDELRRWIGPPLHASFEQLLDDPRLADQAVAHYRQR
YWETGLYENTVFPGIPELLSDLKAAGIRLFVATSKIRPPTERILAHFGLQPFFEAIAAVDPDDRDGNKAMVIGTLLPTLG
ADRAWTVMVGDTIFDIHGARAHRMPCIAVTYGYGEYRDLYLAAPLALASTVGTLRELLSADDPHSVRYRHSRPGTGSQHD
PTA
>Mature_242_residues
GMKRQHRFRAILFDLDGTLTDPFTGIARSVNYALQRLGHPLLSDDELRRWIGPPLHASFEQLLDDPRLADQAVAHYRQRY
WETGLYENTVFPGIPELLSDLKAAGIRLFVATSKIRPPTERILAHFGLQPFFEAIAAVDPDDRDGNKAMVIGTLLPTLGA
DRAWTVMVGDTIFDIHGARAHRMPCIAVTYGYGEYRDLYLAAPLALASTVGTLRELLSADDPHSVRYRHSRPGTGSQHDP
TA

Specific function: Specifically dephosphorylates nucleoside 5'- monophosphates to nucleosides and inorganic phosphate. Displays high activity toward 5'-UMP and 5'-IMP, significant activity against 5'-XMP and 5'-TMP, and low activity against 5'-CMP [H]

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=248, Percent_Identity=27.4193548387097, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.5 [H]

Molecular weight: Translated: 27101; Mature: 26970

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMKRQHRFRAILFDLDGTLTDPFTGIARSVNYALQRLGHPLLSDDELRRWIGPPLHASF
CCCCHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHH
EQLLDDPRLADQAVAHYRQRYWETGLYENTVFPGIPELLSDLKAAGIRLFVATSKIRPPT
HHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEEEEEECCCCCHH
ERILAHFGLQPFFEAIAAVDPDDRDGNKAMVIGTLLPTLGADRAWTVMVGDTIFDIHGAR
HHHHHHHCHHHHHHHHHHCCCCCCCCCEEEEEEEHHHHCCCCCEEEEEECCEEEECCCCH
AHRMPCIAVTYGYGEYRDLYLAAPLALASTVGTLRELLSADDPHSVRYRHSRPGTGSQHD
HHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCC
PTA
CCC
>Mature Secondary Structure 
GMKRQHRFRAILFDLDGTLTDPFTGIARSVNYALQRLGHPLLSDDELRRWIGPPLHASF
CCCHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHH
EQLLDDPRLADQAVAHYRQRYWETGLYENTVFPGIPELLSDLKAAGIRLFVATSKIRPPT
HHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEEEEEECCCCCHH
ERILAHFGLQPFFEAIAAVDPDDRDGNKAMVIGTLLPTLGADRAWTVMVGDTIFDIHGAR
HHHHHHHCHHHHHHHHHHCCCCCCCCCEEEEEEEHHHHCCCCCEEEEEECCEEEECCCCH
AHRMPCIAVTYGYGEYRDLYLAAPLALASTVGTLRELLSADDPHSVRYRHSRPGTGSQHD
HHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCC
PTA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]