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The map label for this gene is fusA [H]

Identifier: 222526151

GI number: 222526151

Start: 3591835

End: 3593946

Strand: Direct

Name: fusA [H]

Synonym: Chy400_2908

Alternate gene names: 222526151

Gene position: 3591835-3593946 (Clockwise)

Preceding gene: 222526150

Following gene: 222526152

Centisome position: 68.17

GC content: 57.29

Gene sequence:

>2112_bases
ATGCGAGCGTTCGAATCGGAAAGGATCCACAACATCGGCATATTCGGTCACCTCGGCAGCGGGAAGACAACGCTGGCCGA
GGCAATGCTGATGACCGCCCACGCCATCCCGCGGATGGGGCGCGTCGAGGACGGGTCTACGACGAGTGACTACGACCCCG
ACGAGCACCGACGCGGGATGTCTATTTCGCTGAGTGTGCTGCCGCTGGAGTGGAACGGCGATAAGATCAACCTGATCGAC
GTGCCCGGTGCCGCTGATTTTGCCGGTGAAGCTGCCGCCGCGATGCGTATCATCGATGGCGCATTGATTGTGCTCGATGC
AAGTGCCGGTGTTGAAGTCGGTACCGAACTCTTCTGGGAGATGGCCGTCCAACAGCGCATTCCACGCATCCTGTTTGTCA
ATAAACTCGACCGCGAAAATGCGAATTTCTACCGCGTGATCGAGCAGGCCCGTGAGCTGCTCGATGCCGCTGTTATTCCG
ATGCAGATTCCTATCGGTGCCGGTAAAGAGTTCAAGGGTATTATCTCGCTGCGTCAACAACGAGCCTGGCTGACCAGCCC
GAAACACGATGGCGGCTACATCGAGGCTGATGTGCCGGCGGAACTCAATGATCTGATGCACGAGTGGCGCACCGCGCTGA
TTGATAAAATTGCCGCAACCAATGATCATCTGATCGAACGGTATCTTGAAGGCGGTGAAGATGCGCTGACCCGCGAGGAA
TTGCTGCTCGGTCTGCGCACCGGTATTGCCGATGGCTCAATCGTGCCGGTGTTCTGCGGTTCAGCAACTGAAGTCGTGGG
GATTGCCCAGCTCCTTAACGGAATCGTCGACTCAATTCCTTCCGCAGGCCGCAAGACAACCACCGCCACCGATCTGAACA
CCGACCAGGAGGTTGAGCTACGTCCAGACCGCGCCGAACCACTGGCAGCACTGGTCTTTAAGACGGTCTCTGACACCTAC
GGCAAGCTTAGCTATTTCCGCGTCTTTTCGGGTGAGGTGCGGGCCGGTATGACGCTCATGAATGCACGCACCCGTAAGGA
AGAGCGCGTCGCCCACGTGTATATTGTGCGTGGTAAAGAGCAGATTGAGGTTGAGTCGGTCGGGCCTGGCGATATTGGTT
TGTTAACGAAGCTCGGTGATACGCAAACCAACGATACCCTCTGCCTGTCGTCGCGACCACTCGCACTTACTCCTATCCAA
TTCCCGGCACCGGCCTTCATTGCAACGGTCAAACCACGCAGCCGTTCCGACCTTGACAAACTCAGCTCTGCGCTCACCAG
GATGACCGAAGAGGATCCGTCTCTCCACGTATCCCGCGATCCGCGTACCGGCGAAGCGTTGCTCAGTGGTCTAAGCGAGA
CGCATCTGCAGATCATTGCCGAACGGATGAAGCGCAAGTTCGATGTGAATATTGACCTCGAACTGCCCCGCATCCCCTAC
CGCGAGACGATCCGTAGTGTTGCGACTGCTCAGTACCGCCACAAGAAACAAACCGGTGGGGCCGGTCAGTTCGCCGATGT
CGCGCTGCGTGTTGAGCCGCTCCCACCCGATCCGAACCGTGAAGACCCGCTTGAGTTCGTGAACGAGATTGTGGGTGGCG
TAATCTCGCGCGGGTTTATGCCGGCAATCGAAAAGGGTATTCGCGAGGCGATGGAAGAAGGCATCATTTCGGGTAATCCG
GTTGTCGATGTACGGGCTGCTGTCTATGACGGGAAAGAACATCCGGTTGACTCGAAGGAAATTGCCTTCAAGACCGCTGC
GAAAGAGGCGTTCCGCCTGGCTGCCCAAAAGGCCGGTGTGATCATTCTCGAACCGATTTACAACATGGAGATCATTGTTC
CCGATCAGTTTGCCGGCGATGTGATGAGTGATATGAGCACGCGCCGTGGTCGGGTACAGGGGATGATGCCAACCGGCACC
GGCAAGACGGTGATCCACGCCCAGGCTCCTCTGGTCGAGATTCAACGCTACGCAACCGATTTACGAGGGATGACCCAGGG
CCGTGGCCGCTTCTCGATCAGTTTCGCGGGTTACGAAGAGGTGCCGCCACATCTGGTTAACCAGATTGTCGAAGCGCACA
AGAAAGAACTGGAAGCGGCGCATAGCCACTGA

Upstream 100 bases:

>100_bases
TGACGCAACCATCACAACCCTGATAATCTGTTCTTAACAACTTTATTTTCCTTTCCAGCACACGCCCAACGGGCGCGGGC
TTGCGTCAAGGAGGACAGCT

Downstream 100 bases:

>100_bases
GACGGTTCTGTCCGAAGGGATCAATGTAGGGGCGGGTTTGGAATAGGTTGAGAGTAGGGGCGGGTTTGGAACCCGCCCCT
ACAGCATTGGGCCTATGGAA

Product: small GTP-binding protein

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 703; Mature: 703

Protein sequence:

>703_residues
MRAFESERIHNIGIFGHLGSGKTTLAEAMLMTAHAIPRMGRVEDGSTTSDYDPDEHRRGMSISLSVLPLEWNGDKINLID
VPGAADFAGEAAAAMRIIDGALIVLDASAGVEVGTELFWEMAVQQRIPRILFVNKLDRENANFYRVIEQARELLDAAVIP
MQIPIGAGKEFKGIISLRQQRAWLTSPKHDGGYIEADVPAELNDLMHEWRTALIDKIAATNDHLIERYLEGGEDALTREE
LLLGLRTGIADGSIVPVFCGSATEVVGIAQLLNGIVDSIPSAGRKTTTATDLNTDQEVELRPDRAEPLAALVFKTVSDTY
GKLSYFRVFSGEVRAGMTLMNARTRKEERVAHVYIVRGKEQIEVESVGPGDIGLLTKLGDTQTNDTLCLSSRPLALTPIQ
FPAPAFIATVKPRSRSDLDKLSSALTRMTEEDPSLHVSRDPRTGEALLSGLSETHLQIIAERMKRKFDVNIDLELPRIPY
RETIRSVATAQYRHKKQTGGAGQFADVALRVEPLPPDPNREDPLEFVNEIVGGVISRGFMPAIEKGIREAMEEGIISGNP
VVDVRAAVYDGKEHPVDSKEIAFKTAAKEAFRLAAQKAGVIILEPIYNMEIIVPDQFAGDVMSDMSTRRGRVQGMMPTGT
GKTVIHAQAPLVEIQRYATDLRGMTQGRGRFSISFAGYEEVPPHLVNQIVEAHKKELEAAHSH

Sequences:

>Translated_703_residues
MRAFESERIHNIGIFGHLGSGKTTLAEAMLMTAHAIPRMGRVEDGSTTSDYDPDEHRRGMSISLSVLPLEWNGDKINLID
VPGAADFAGEAAAAMRIIDGALIVLDASAGVEVGTELFWEMAVQQRIPRILFVNKLDRENANFYRVIEQARELLDAAVIP
MQIPIGAGKEFKGIISLRQQRAWLTSPKHDGGYIEADVPAELNDLMHEWRTALIDKIAATNDHLIERYLEGGEDALTREE
LLLGLRTGIADGSIVPVFCGSATEVVGIAQLLNGIVDSIPSAGRKTTTATDLNTDQEVELRPDRAEPLAALVFKTVSDTY
GKLSYFRVFSGEVRAGMTLMNARTRKEERVAHVYIVRGKEQIEVESVGPGDIGLLTKLGDTQTNDTLCLSSRPLALTPIQ
FPAPAFIATVKPRSRSDLDKLSSALTRMTEEDPSLHVSRDPRTGEALLSGLSETHLQIIAERMKRKFDVNIDLELPRIPY
RETIRSVATAQYRHKKQTGGAGQFADVALRVEPLPPDPNREDPLEFVNEIVGGVISRGFMPAIEKGIREAMEEGIISGNP
VVDVRAAVYDGKEHPVDSKEIAFKTAAKEAFRLAAQKAGVIILEPIYNMEIIVPDQFAGDVMSDMSTRRGRVQGMMPTGT
GKTVIHAQAPLVEIQRYATDLRGMTQGRGRFSISFAGYEEVPPHLVNQIVEAHKKELEAAHSH
>Mature_703_residues
MRAFESERIHNIGIFGHLGSGKTTLAEAMLMTAHAIPRMGRVEDGSTTSDYDPDEHRRGMSISLSVLPLEWNGDKINLID
VPGAADFAGEAAAAMRIIDGALIVLDASAGVEVGTELFWEMAVQQRIPRILFVNKLDRENANFYRVIEQARELLDAAVIP
MQIPIGAGKEFKGIISLRQQRAWLTSPKHDGGYIEADVPAELNDLMHEWRTALIDKIAATNDHLIERYLEGGEDALTREE
LLLGLRTGIADGSIVPVFCGSATEVVGIAQLLNGIVDSIPSAGRKTTTATDLNTDQEVELRPDRAEPLAALVFKTVSDTY
GKLSYFRVFSGEVRAGMTLMNARTRKEERVAHVYIVRGKEQIEVESVGPGDIGLLTKLGDTQTNDTLCLSSRPLALTPIQ
FPAPAFIATVKPRSRSDLDKLSSALTRMTEEDPSLHVSRDPRTGEALLSGLSETHLQIIAERMKRKFDVNIDLELPRIPY
RETIRSVATAQYRHKKQTGGAGQFADVALRVEPLPPDPNREDPLEFVNEIVGGVISRGFMPAIEKGIREAMEEGIISGNP
VVDVRAAVYDGKEHPVDSKEIAFKTAAKEAFRLAAQKAGVIILEPIYNMEIIVPDQFAGDVMSDMSTRRGRVQGMMPTGT
GKTVIHAQAPLVEIQRYATDLRGMTQGRGRFSISFAGYEEVPPHLVNQIVEAHKKELEAAHSH

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=707, Percent_Identity=33.6633663366337, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI19923640, Length=721, Percent_Identity=30.7905686546463, Blast_Score=318, Evalue=1e-86,
Organism=Homo sapiens, GI25306283, Length=444, Percent_Identity=31.5315315315315, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI25306287, Length=284, Percent_Identity=36.9718309859155, Blast_Score=167, Evalue=4e-41,
Organism=Homo sapiens, GI157426893, Length=226, Percent_Identity=27.8761061946903, Blast_Score=83, Evalue=1e-15,
Organism=Homo sapiens, GI4503483, Length=147, Percent_Identity=34.0136054421769, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=36.5671641791045, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI217272894, Length=138, Percent_Identity=33.3333333333333, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI217272892, Length=138, Percent_Identity=33.3333333333333, Blast_Score=76, Evalue=1e-13,
Organism=Escherichia coli, GI1789738, Length=700, Percent_Identity=37.4285714285714, Blast_Score=477, Evalue=1e-136,
Organism=Escherichia coli, GI1790835, Length=473, Percent_Identity=26.215644820296, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI48994988, Length=177, Percent_Identity=31.638418079096, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=685, Percent_Identity=32.1167883211679, Blast_Score=358, Evalue=4e-99,
Organism=Caenorhabditis elegans, GI17556745, Length=724, Percent_Identity=24.7237569060773, Blast_Score=199, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI17552882, Length=805, Percent_Identity=21.7391304347826, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=158, Percent_Identity=32.2784810126582, Blast_Score=84, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=152, Percent_Identity=30.2631578947368, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=33.0827067669173, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=33.0827067669173, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=702, Percent_Identity=33.3333333333333, Blast_Score=375, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6322359, Length=520, Percent_Identity=28.0769230769231, Blast_Score=213, Evalue=9e-56,
Organism=Saccharomyces cerevisiae, GI6324707, Length=816, Percent_Identity=23.1617647058824, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320593, Length=816, Percent_Identity=23.1617647058824, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322675, Length=142, Percent_Identity=35.2112676056338, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24582462, Length=698, Percent_Identity=34.3839541547278, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI221458488, Length=724, Percent_Identity=28.1767955801105, Blast_Score=274, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24585711, Length=150, Percent_Identity=34, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24585713, Length=150, Percent_Identity=34, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24585709, Length=150, Percent_Identity=34, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21357743, Length=136, Percent_Identity=34.5588235294118, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI78706572, Length=153, Percent_Identity=30.0653594771242, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI28574573, Length=159, Percent_Identity=32.0754716981132, Blast_Score=67, Evalue=6e-11,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 77154; Mature: 77154

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAFESERIHNIGIFGHLGSGKTTLAEAMLMTAHAIPRMGRVEDGSTTSDYDPDEHRRGM
CCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCC
SISLSVLPLEWNGDKINLIDVPGAADFAGEAAAAMRIIDGALIVLDASAGVEVGTELFWE
EEEEEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHH
MAVQQRIPRILFVNKLDRENANFYRVIEQARELLDAAVIPMQIPIGAGKEFKGIISLRQQ
HHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHH
RAWLTSPKHDGGYIEADVPAELNDLMHEWRTALIDKIAATNDHLIERYLEGGEDALTREE
HHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHH
LLLGLRTGIADGSIVPVFCGSATEVVGIAQLLNGIVDSIPSAGRKTTTATDLNTDQEVEL
HHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEEE
RPDRAEPLAALVFKTVSDTYGKLSYFRVFSGEVRAGMTLMNARTRKEERVAHVYIVRGKE
CCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCHHHCCHHHHHHHHHHHCEEEEEEEECCC
QIEVESVGPGDIGLLTKLGDTQTNDTLCLSSRPLALTPIQFPAPAFIATVKPRSRSDLDK
EEEEECCCCCCEEEEEECCCCCCCCEEEECCCCCEECCCCCCCCCEEEEECCCCCCHHHH
LSSALTRMTEEDPSLHVSRDPRTGEALLSGLSETHLQIIAERMKRKFDVNIDLELPRIPY
HHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCH
RETIRSVATAQYRHKKQTGGAGQFADVALRVEPLPPDPNREDPLEFVNEIVGGVISRGFM
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCH
PAIEKGIREAMEEGIISGNPVVDVRAAVYDGKEHPVDSKEIAFKTAAKEAFRLAAQKAGV
HHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCE
IILEPIYNMEIIVPDQFAGDVMSDMSTRRGRVQGMMPTGTGKTVIHAQAPLVEIQRYATD
EEEECCCCCEEEECCHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCHHHHHHHHHH
LRGMTQGRGRFSISFAGYEEVPPHLVNQIVEAHKKELEAAHSH
HHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRAFESERIHNIGIFGHLGSGKTTLAEAMLMTAHAIPRMGRVEDGSTTSDYDPDEHRRGM
CCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCC
SISLSVLPLEWNGDKINLIDVPGAADFAGEAAAAMRIIDGALIVLDASAGVEVGTELFWE
EEEEEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHH
MAVQQRIPRILFVNKLDRENANFYRVIEQARELLDAAVIPMQIPIGAGKEFKGIISLRQQ
HHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHH
RAWLTSPKHDGGYIEADVPAELNDLMHEWRTALIDKIAATNDHLIERYLEGGEDALTREE
HHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHH
LLLGLRTGIADGSIVPVFCGSATEVVGIAQLLNGIVDSIPSAGRKTTTATDLNTDQEVEL
HHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEEE
RPDRAEPLAALVFKTVSDTYGKLSYFRVFSGEVRAGMTLMNARTRKEERVAHVYIVRGKE
CCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCHHHCCHHHHHHHHHHHCEEEEEEEECCC
QIEVESVGPGDIGLLTKLGDTQTNDTLCLSSRPLALTPIQFPAPAFIATVKPRSRSDLDK
EEEEECCCCCCEEEEEECCCCCCCCEEEECCCCCEECCCCCCCCCEEEEECCCCCCHHHH
LSSALTRMTEEDPSLHVSRDPRTGEALLSGLSETHLQIIAERMKRKFDVNIDLELPRIPY
HHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCH
RETIRSVATAQYRHKKQTGGAGQFADVALRVEPLPPDPNREDPLEFVNEIVGGVISRGFM
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCH
PAIEKGIREAMEEGIISGNPVVDVRAAVYDGKEHPVDSKEIAFKTAAKEAFRLAAQKAGV
HHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCE
IILEPIYNMEIIVPDQFAGDVMSDMSTRRGRVQGMMPTGTGKTVIHAQAPLVEIQRYATD
EEEECCCCCEEEECCHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCHHHHHHHHHH
LRGMTQGRGRFSISFAGYEEVPPHLVNQIVEAHKKELEAAHSH
HHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA