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The map label for this gene is cobD [H]

Identifier: 222526012

GI number: 222526012

Start: 3412295

End: 3413380

Strand: Reverse

Name: cobD [H]

Synonym: Chy400_2768

Alternate gene names: 222526012

Gene position: 3413380-3412295 (Counterclockwise)

Preceding gene: 222526013

Following gene: 222526011

Centisome position: 64.78

GC content: 62.62

Gene sequence:

>1086_bases
ATGGATGCGTACAAACCGGTAGTGCACGGTGCGCTCGACGAGGCTGAACTGGCCCGGCTTGGCCTGCGCGCCGACCATCT
GATCGACTTCAGCAGCAACATCAACCCCTTCGGGCCACCAGAATCGGTTCTACAGGCGCTGCGTACCCTCAATCCAGCCC
CCTACCCTGATCGCAGTTGTCTGCACCTGCGCAGGCATCTGGCAGCCATCCATCACTGTCAGCCGGAGGCCGTTCTGGTG
GGAAACGGGGCGAATGAGCTGATCCACCTGGTAGCCCGTGCCCTCGGTGAACCCGGTGCCACAGCTCTGGTCGTAGCCCC
AACCTACGGCGAATACGCCTACGCCTCACACCTGGCCGGCATGCGGGTGATCGAGATACGGAGCACCGTTGGCAACGGCT
TCCAACCCGATCTCGCCGAACTCCTGCTCACCATCGAACGACTGCGTCCACGGCTGATCTGGCTGTGCTCCCCCAACAAT
CCCACCGGGGTCAATCTGTCGGTAACGACGATCACAGAACTGGCTCATACCTGCGCACTTCATCAAGGCTGGCTCATCCT
CGACCGGGCCTATCTGGCGTTGGGTCGTGACGAGACCGGTCACGATCCACTCGACCACAACAGCCCGTCGCGCCTGATCC
GTATCTACTCGCTCACCAAAAGCTATGCGGTTGCCGGCCTCCGCATTGGCTACATGATTGCCGATCCCGACGTGATAGCC
GCAACTGCCCGCTTCCAACCGGCGTGGAGCGTGAGCAGTGCAGCGCAGGCTGCCGCCGCAGCGATGCTGCACGATACCGG
CTTTCTTGCCACCACGCTCCCGGCGCTATGGGATGCCAGCAGCGAACTGGCCCAAAACCTGCGCCAGATCGGCCTGACCG
TCTGGCGCGCCCATCTGCCGTTTATGCTTGTCCATTGCGACAATGCGGCACTGACCCGCCAGCAACTGCTGGTGCGCGGG
TGCGTTGTGCGCGACTGTACATCGTTCGGGTTACCGGAATGGGTGCGGGTTGCGCCCCGACGCCCATCCGAGAATGCCCG
GTTACTGGCAGCCTGGCGCGATGTCCACGCCAGAACTCAGGTATAA

Upstream 100 bases:

>100_bases
ATCTCCTGGGTCGGGTCAATCAGCAGATCGCCGCCGCCGCCGCCGAAGTCTATCTGGTGGTATGCGGCATACCAGTCGAA
TTACGCTCTCTGGAGGCAGC

Downstream 100 bases:

>100_bases
GCCCGCGCTGTTACAAACGAGAGGATTGACAATGCAAGCGCCTGTACTCATGGTCGTCGGAACTGCATCATCAGTTGGCA
AGAGCACCCTGGTCACCGCC

Product: class I and II aminotransferase

Products: NA

Alternate protein names: L-threonine-O-3-phosphate decarboxylase [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MDAYKPVVHGALDEAELARLGLRADHLIDFSSNINPFGPPESVLQALRTLNPAPYPDRSCLHLRRHLAAIHHCQPEAVLV
GNGANELIHLVARALGEPGATALVVAPTYGEYAYASHLAGMRVIEIRSTVGNGFQPDLAELLLTIERLRPRLIWLCSPNN
PTGVNLSVTTITELAHTCALHQGWLILDRAYLALGRDETGHDPLDHNSPSRLIRIYSLTKSYAVAGLRIGYMIADPDVIA
ATARFQPAWSVSSAAQAAAAAMLHDTGFLATTLPALWDASSELAQNLRQIGLTVWRAHLPFMLVHCDNAALTRQQLLVRG
CVVRDCTSFGLPEWVRVAPRRPSENARLLAAWRDVHARTQV

Sequences:

>Translated_361_residues
MDAYKPVVHGALDEAELARLGLRADHLIDFSSNINPFGPPESVLQALRTLNPAPYPDRSCLHLRRHLAAIHHCQPEAVLV
GNGANELIHLVARALGEPGATALVVAPTYGEYAYASHLAGMRVIEIRSTVGNGFQPDLAELLLTIERLRPRLIWLCSPNN
PTGVNLSVTTITELAHTCALHQGWLILDRAYLALGRDETGHDPLDHNSPSRLIRIYSLTKSYAVAGLRIGYMIADPDVIA
ATARFQPAWSVSSAAQAAAAAMLHDTGFLATTLPALWDASSELAQNLRQIGLTVWRAHLPFMLVHCDNAALTRQQLLVRG
CVVRDCTSFGLPEWVRVAPRRPSENARLLAAWRDVHARTQV
>Mature_361_residues
MDAYKPVVHGALDEAELARLGLRADHLIDFSSNINPFGPPESVLQALRTLNPAPYPDRSCLHLRRHLAAIHHCQPEAVLV
GNGANELIHLVARALGEPGATALVVAPTYGEYAYASHLAGMRVIEIRSTVGNGFQPDLAELLLTIERLRPRLIWLCSPNN
PTGVNLSVTTITELAHTCALHQGWLILDRAYLALGRDETGHDPLDHNSPSRLIRIYSLTKSYAVAGLRIGYMIADPDVIA
ATARFQPAWSVSSAAQAAAAAMLHDTGFLATTLPALWDASSELAQNLRQIGLTVWRAHLPFMLVHCDNAALTRQQLLVRG
CVVRDCTSFGLPEWVRVAPRRPSENARLLAAWRDVHARTQV

Specific function: Decarboxylates L-threonine-O-3-phosphate to yield (R)-1- amino-2-propanol O-2-phosphate, the precursor for the linkage between the nucleotide loop and the corrin ring in cobalamin [H]

COG id: COG0079

COG function: function code E; Histidinol-phosphate/aromatic aminotransferase and cobyric acid decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788332, Length=229, Percent_Identity=30.1310043668122, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788627, Length=232, Percent_Identity=29.3103448275862, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322075, Length=231, Percent_Identity=26.8398268398268, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR005860
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =4.1.1.81 [H]

Molecular weight: Translated: 39475; Mature: 39475

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS00105 AA_TRANSFER_CLASS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAYKPVVHGALDEAELARLGLRADHLIDFSSNINPFGPPESVLQALRTLNPAPYPDRSC
CCCCCCHHHCCCCHHHHHHHCCCHHHEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCHHH
LHLRRHLAAIHHCQPEAVLVGNGANELIHLVARALGEPGATALVVAPTYGEYAYASHLAG
HHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHCC
MRVIEIRSTVGNGFQPDLAELLLTIERLRPRLIWLCSPNNPTGVNLSVTTITELAHTCAL
EEEEEEEHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHH
HQGWLILDRAYLALGRDETGHDPLDHNSPSRLIRIYSLTKSYAVAGLRIGYMIADPDVIA
HCCCCEEHHHHHHCCCCCCCCCCCCCCCHHHEEEHHHHHHHHHHHHEEEEEEEECCCCEE
ATARFQPAWSVSSAAQAAAAAMLHDTGFLATTLPALWDASSELAQNLRQIGLTVWRAHLP
EECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
FMLVHCDNAALTRQQLLVRGCVVRDCTSFGLPEWVRVAPRRPSENARLLAAWRDVHARTQ
EEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEHHHHHHHHHCC
V
C
>Mature Secondary Structure
MDAYKPVVHGALDEAELARLGLRADHLIDFSSNINPFGPPESVLQALRTLNPAPYPDRSC
CCCCCCHHHCCCCHHHHHHHCCCHHHEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCHHH
LHLRRHLAAIHHCQPEAVLVGNGANELIHLVARALGEPGATALVVAPTYGEYAYASHLAG
HHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHCC
MRVIEIRSTVGNGFQPDLAELLLTIERLRPRLIWLCSPNNPTGVNLSVTTITELAHTCAL
EEEEEEEHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHH
HQGWLILDRAYLALGRDETGHDPLDHNSPSRLIRIYSLTKSYAVAGLRIGYMIADPDVIA
HCCCCEEHHHHHHCCCCCCCCCCCCCCCHHHEEEHHHHHHHHHHHHEEEEEEEECCCCEE
ATARFQPAWSVSSAAQAAAAAMLHDTGFLATTLPALWDASSELAQNLRQIGLTVWRAHLP
EECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
FMLVHCDNAALTRQQLLVRGCVVRDCTSFGLPEWVRVAPRRPSENARLLAAWRDVHARTQ
EEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEHHHHHHHHHCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]