Click here to switch to the map view.

The map label for this gene is pspA [C]

Identifier: 222525735

GI number: 222525735

Start: 3130522

End: 3131184

Strand: Direct

Name: pspA [C]

Synonym: Chy400_2486

Alternate gene names: 222525735

Gene position: 3130522-3131184 (Clockwise)

Preceding gene: 222525733

Following gene: 222525737

Centisome position: 59.41

GC content: 56.56

Gene sequence:

>663_bases
ATGTCCATTCTCGGTCGAATTAAAGATCTGATCAGTGCCAACATTAACGCCATGCTCGACAAAGCGGAGGATCCGGAGAA
GATGGCGAACGAGTACCTGCGCCAGTTAACGAACGAGCTATACGAGGCGCGAACGGGGGTTGCTCAGGCGATGGCTGATG
AAACCCGCCTTGAACAGCGGCGTATTGCTGCCGAGGGTGAGGCCGAGCAGTGGCAAATTAAGGCCGAGCGTGCGTTACGT
GCTGGTGACGAAGCGTTAGCGAAAGCCGCATTGGCGCGTAAGGTGCAGGCGCAGAAACTGGCTGAGCAATACCGTGCCCA
GGAAAAGGCTCAGGAAGAGCAGGTGGAAGCTCTCCAGCAGGCATTGATTGACCTCGAAACCCGGATCGCTGAGGTTAAAG
CACGCAAAGAGCTGATTATCGCCAAAAAGAACCGTGCTCAGACCCAAGAGGCACTTCAGCGCACGGCGGCTTCGATTGGT
CGGGTCAGTGCCCTTGATAAGCTCGATCAGCTCGAAGAAAAGGTGGATGACCGCCTGGCCCGCGCCGAAGCAATGGCGAA
ACTGGAAGGGGACTCGCTCGAAGCACGCTTCCGTGATCTGGAAAAAGAGACCGAGGTGGATAGCGAGCTGGCTGAGCTGA
AGCGTAAACTCGGCTTGAGCTGA

Upstream 100 bases:

>100_bases
AGCGGGATCGGCGTGCTACAATGCAGGCAGCATCTCATCTCCGGACACGTATCTCTACAGCCAGGGTCTGTTTCATGTCG
CAACCAAAGGAGGAGCGTCC

Downstream 100 bases:

>100_bases
TTGACATTCGTCTCCAATGCACCTGGTGATGGGGCGGGCTGATGCTCGCCCCATTGCTACAGCTTATACCGCTGGCTCGT
AACAGGTCCGGCAGCGCGCT

Product: phage shock protein PspA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MSILGRIKDLISANINAMLDKAEDPEKMANEYLRQLTNELYEARTGVAQAMADETRLEQRRIAAEGEAEQWQIKAERALR
AGDEALAKAALARKVQAQKLAEQYRAQEKAQEEQVEALQQALIDLETRIAEVKARKELIIAKKNRAQTQEALQRTAASIG
RVSALDKLDQLEEKVDDRLARAEAMAKLEGDSLEARFRDLEKETEVDSELAELKRKLGLS

Sequences:

>Translated_220_residues
MSILGRIKDLISANINAMLDKAEDPEKMANEYLRQLTNELYEARTGVAQAMADETRLEQRRIAAEGEAEQWQIKAERALR
AGDEALAKAALARKVQAQKLAEQYRAQEKAQEEQVEALQQALIDLETRIAEVKARKELIIAKKNRAQTQEALQRTAASIG
RVSALDKLDQLEEKVDDRLARAEAMAKLEGDSLEARFRDLEKETEVDSELAELKRKLGLS
>Mature_219_residues
SILGRIKDLISANINAMLDKAEDPEKMANEYLRQLTNELYEARTGVAQAMADETRLEQRRIAAEGEAEQWQIKAERALRA
GDEALAKAALARKVQAQKLAEQYRAQEKAQEEQVEALQQALIDLETRIAEVKARKELIIAKKNRAQTQEALQRTAASIGR
VSALDKLDQLEEKVDDRLARAEAMAKLEGDSLEARFRDLEKETEVDSELAELKRKLGLS

Specific function: The Phage Shock Protein (Psp) Operon (Pspabce) May Play A Significant Role In The Competition For Survival Under Nutrient- Or Energy-Limited Conditions. Pspa Has A Role As A Negative Regulator, Is Required For Efficient Translocation And Has An Unknown Fu

COG id: COG1842

COG function: function code KT; Phage shock protein A (IM30), suppresses sigma54-dependent transcription

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PspA/IM30 family [H]

Homologues:

Organism=Escherichia coli, GI2367118, Length=217, Percent_Identity=29.0322580645161, Blast_Score=87, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007157 [H]

Pfam domain/function: PF04012 PspA_IM30 [H]

EC number: NA

Molecular weight: Translated: 24755; Mature: 24624

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILGRIKDLISANINAMLDKAEDPEKMANEYLRQLTNELYEARTGVAQAMADETRLEQR
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIAAEGEAEQWQIKAERALRAGDEALAKAALARKVQAQKLAEQYRAQEKAQEEQVEALQQ
HHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALIDLETRIAEVKARKELIIAKKNRAQTQEALQRTAASIGRVSALDKLDQLEEKVDDRLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAEAMAKLEGDSLEARFRDLEKETEVDSELAELKRKLGLS
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SILGRIKDLISANINAMLDKAEDPEKMANEYLRQLTNELYEARTGVAQAMADETRLEQR
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIAAEGEAEQWQIKAERALRAGDEALAKAALARKVQAQKLAEQYRAQEKAQEEQVEALQQ
HHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALIDLETRIAEVKARKELIIAKKNRAQTQEALQRTAASIGRVSALDKLDQLEEKVDDRLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAEAMAKLEGDSLEARFRDLEKETEVDSELAELKRKLGLS
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]