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The map label for this gene is rsmG [H]

Identifier: 222525733

GI number: 222525733

Start: 3128093

End: 3129421

Strand: Direct

Name: rsmG [H]

Synonym: Chy400_2484

Alternate gene names: 222525733

Gene position: 3128093-3129421 (Clockwise)

Preceding gene: 222525732

Following gene: 222525735

Centisome position: 59.37

GC content: 58.47

Gene sequence:

>1329_bases
ATGACCACTGTTCGCCGCCTGGGGATTTATGGAGGTACGTTCGACCCAATTCATTTCGGTCATCTCGCCATTGCCGAAGA
AGTGCGCTGGGTCTGTGACCTTGATCAGGTTCTGATTATTCCGGCTGCTGCCCAGCCTCTAAAGCCGACCCACAGTGCTG
CCCCCCATCACCGGTTGGCGATGGTCAGGCTGGCCTGTGCCGGGAATGCTGCACTGATTCCCTCGCCGCTTGAATTGGAA
CGACCACCACCCTCGTATACCATCGATACGCTCCGCATTTGTCAGGAGCGCTATGGTGTTGGAGTGCATCTCACCCTTAT
CGTTGGTGCCGATGCTGCCGGCGACCTGCCCCGCTGGCGCGATCCGGATCAGATTGCCCGTATCGCCCATCTGGCAGTTG
TTGAACGGCCCGGGCATCTCTTCGATCCGGCAACCCTGCTTGCTGCCGTGCCCGCTTTCACTGGTCGGATAACGGTCATT
AAGGGGCCTCAACTTGCCATCTCAAGCACCGATCTGCGCCACCGCCTTGCCACCGGTCGTCCGGTACGCTACCAGTTGCC
AGACGCCGTTCTTGATTACATTACTCGCCCGCCTGTATCAAGCCGGGGAAGCATGACCAATAGCACTGCCCATCTGCTCA
GAAAAACCACCGCTGATTGGGGTCTGTCGCTGAACGATCACCAACTGGCGCAGTTTGAACGCTATCTGGACGAGCTGATG
ACCTGGAACGAACGCTTTAACCTGACTGCCGTTCGTGACCGGGCTGCGATGGTTCGCCGGCATCTGCTCGACTCGCTCTG
GTTGGCCAGGGTCTGGGAAACACCACCGGATAGTTTGATCGATATTGGGAGCGGTGCCGGCTTCCCGGCGCTGCCGCTCA
AAATCCTCTATCCTGCGCTGCCGGTTACTCTGGTAGAGGCTACCGGCAAAAAGGCCACGTTTCTGCGTCACGTCATTGAT
TGCCTTCAGTTGCACAATATTCGAGTGTTAAACGAGCGCATTGAAACCGTCGGCCAAAACCCCACCGAACGCGAACACTA
TCAGGTGGTGACTGCTCGTGCCGTCGCCGAATTACGGGTGTTGCTCGAATACGCCTTGCCACTCTTGCGCCTCGGCGGGC
ATCTGCTTGCTCCCAAAGGGCGGCATCCAGAAGTAGAAATTGCTGCTGCCCAGAATGCCCTCGCCCTGCTTGGTGGTGAA
GTCAGCGCCTGCCTGCCGGTCGAGATTCCTGATGAAGAGCCACGTAGTCTGGTGATTGTAACCAAAACTGCTCCTACTCC
CAACCGCTTCCCCCGCGCCGTTGGCATCCCGGCGCGCCGGCCTTTGTAG

Upstream 100 bases:

>100_bases
CGATGACCTCCATCCCGATGATCAGAACGATTTGGTCTGGCTCGTCTGGCACCTTTTTCAGGAAGCACGCGAGCTGAGTG
AAGAATAAGCTGCGCAAGCC

Downstream 100 bases:

>100_bases
AGAGTGATACCCGCCGGCATGAGCTTATGTCGTGCGTACCTCAAACCAACGTCACCCCGCCGCGCCACCTGATCACCATC
GTGCGCAAAACCGGTATCAC

Product: methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTTVRRLGIYGGTFDPIHFGHLAIAEEVRWVCDLDQVLIIPAAAQPLKPTHSAAPHHRLAMVRLACAGNAALIPSPLELE
RPPPSYTIDTLRICQERYGVGVHLTLIVGADAAGDLPRWRDPDQIARIAHLAVVERPGHLFDPATLLAAVPAFTGRITVI
KGPQLAISSTDLRHRLATGRPVRYQLPDAVLDYITRPPVSSRGSMTNSTAHLLRKTTADWGLSLNDHQLAQFERYLDELM
TWNERFNLTAVRDRAAMVRRHLLDSLWLARVWETPPDSLIDIGSGAGFPALPLKILYPALPVTLVEATGKKATFLRHVID
CLQLHNIRVLNERIETVGQNPTEREHYQVVTARAVAELRVLLEYALPLLRLGGHLLAPKGRHPEVEIAAAQNALALLGGE
VSACLPVEIPDEEPRSLVIVTKTAPTPNRFPRAVGIPARRPL

Sequences:

>Translated_442_residues
MTTVRRLGIYGGTFDPIHFGHLAIAEEVRWVCDLDQVLIIPAAAQPLKPTHSAAPHHRLAMVRLACAGNAALIPSPLELE
RPPPSYTIDTLRICQERYGVGVHLTLIVGADAAGDLPRWRDPDQIARIAHLAVVERPGHLFDPATLLAAVPAFTGRITVI
KGPQLAISSTDLRHRLATGRPVRYQLPDAVLDYITRPPVSSRGSMTNSTAHLLRKTTADWGLSLNDHQLAQFERYLDELM
TWNERFNLTAVRDRAAMVRRHLLDSLWLARVWETPPDSLIDIGSGAGFPALPLKILYPALPVTLVEATGKKATFLRHVID
CLQLHNIRVLNERIETVGQNPTEREHYQVVTARAVAELRVLLEYALPLLRLGGHLLAPKGRHPEVEIAAAQNALALLGGE
VSACLPVEIPDEEPRSLVIVTKTAPTPNRFPRAVGIPARRPL
>Mature_441_residues
TTVRRLGIYGGTFDPIHFGHLAIAEEVRWVCDLDQVLIIPAAAQPLKPTHSAAPHHRLAMVRLACAGNAALIPSPLELER
PPPSYTIDTLRICQERYGVGVHLTLIVGADAAGDLPRWRDPDQIARIAHLAVVERPGHLFDPATLLAAVPAFTGRITVIK
GPQLAISSTDLRHRLATGRPVRYQLPDAVLDYITRPPVSSRGSMTNSTAHLLRKTTADWGLSLNDHQLAQFERYLDELMT
WNERFNLTAVRDRAAMVRRHLLDSLWLARVWETPPDSLIDIGSGAGFPALPLKILYPALPVTLVEATGKKATFLRHVIDC
LQLHNIRVLNERIETVGQNPTEREHYQVVTARAVAELRVLLEYALPLLRLGGHLLAPKGRHPEVEIAAAQNALALLGGEV
SACLPVEIPDEEPRSLVIVTKTAPTPNRFPRAVGIPARRPL

Specific function: Specifically methylates the N7 position of a guanosine in 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=184, Percent_Identity=37.5, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786858, Length=205, Percent_Identity=34.1463414634146, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: 2.1.-.-

Molecular weight: Translated: 48697; Mature: 48566

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVRRLGIYGGTFDPIHFGHLAIAEEVRWVCDLDQVLIIPAAAQPLKPTHSAAPHHRLA
CCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHEE
MVRLACAGNAALIPSPLELERPPPSYTIDTLRICQERYGVGVHLTLIVGADAAGDLPRWR
EEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCC
DPDQIARIAHLAVVERPGHLFDPATLLAAVPAFTGRITVIKGPQLAISSTDLRHRLATGR
CHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCEEEECHHHHHHHHCCC
PVRYQLPDAVLDYITRPPVSSRGSMTNSTAHLLRKTTADWGLSLNDHQLAQFERYLDELM
CEEEECCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
TWNERFNLTAVRDRAAMVRRHLLDSLWLARVWETPPDSLIDIGSGAGFPALPLKILYPAL
HHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEECCCCCCCCCCHHHHHHCCC
PVTLVEATGKKATFLRHVIDCLQLHNIRVLNERIETVGQNPTEREHYQVVTARAVAELRV
CEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
LLEYALPLLRLGGHLLAPKGRHPEVEIAAAQNALALLGGEVSACLPVEIPDEEPRSLVIV
HHHHHHHHHHHCCEEECCCCCCCCEEEEECCCHHHHHCCCCCEEEEEECCCCCCCEEEEE
TKTAPTPNRFPRAVGIPARRPL
EECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTVRRLGIYGGTFDPIHFGHLAIAEEVRWVCDLDQVLIIPAAAQPLKPTHSAAPHHRLA
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHEE
MVRLACAGNAALIPSPLELERPPPSYTIDTLRICQERYGVGVHLTLIVGADAAGDLPRWR
EEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCC
DPDQIARIAHLAVVERPGHLFDPATLLAAVPAFTGRITVIKGPQLAISSTDLRHRLATGR
CHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCEEEECHHHHHHHHCCC
PVRYQLPDAVLDYITRPPVSSRGSMTNSTAHLLRKTTADWGLSLNDHQLAQFERYLDELM
CEEEECCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
TWNERFNLTAVRDRAAMVRRHLLDSLWLARVWETPPDSLIDIGSGAGFPALPLKILYPAL
HHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEECCCCCCCCCCHHHHHHCCC
PVTLVEATGKKATFLRHVIDCLQLHNIRVLNERIETVGQNPTEREHYQVVTARAVAELRV
CEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
LLEYALPLLRLGGHLLAPKGRHPEVEIAAAQNALALLGGEVSACLPVEIPDEEPRSLVIV
HHHHHHHHHHHCCEEECCCCCCCCEEEEECCCHHHHHCCCCCEEEEEECCCCCCCEEEEE
TKTAPTPNRFPRAVGIPARRPL
EECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA