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The map label for this gene is fcl [C]

Identifier: 222525537

GI number: 222525537

Start: 2892201

End: 2893208

Strand: Direct

Name: fcl [C]

Synonym: Chy400_2286

Alternate gene names: 222525537

Gene position: 2892201-2893208 (Clockwise)

Preceding gene: 222525536

Following gene: 222525538

Centisome position: 54.89

GC content: 54.96

Gene sequence:

>1008_bases
ATGCATCTCAACAATCATCACTGGCCTGATAGCTATGAGTTCTGGCAGGACAAATCTGTTGTAGTGACGGGTGGTGCCGG
CTTTCTCGGTTCGTATGTAGTCGAAAAGCTACATGAGCGTGGTGCGCGGCGGATCGTTGTTCCACGTTCGCACCAGTACG
ATCTGCGCCAGCTTGAGGCTATTCGCCAGCTTCTAGCCGATGCGCAACCCGATATTGTCATCCATATGGCGGCCCGGGTT
GGCGGTATCGGTGCCAATCGCGATCATCCGGCAGAGTTCTTCTACGATAACCTGATGATGGGCGTACAGCTTTTGCACGA
GAGCTGGCGGTTTGGCGTACAGAAGTTCGTGACGATTGGTACGGTTTGTGCATATCCCAAATATACGCCGGTGCCGTTTA
AGGAAGACGACCTCTGGAACGGCTACCCGGAAGAGACGAACGCGCCATACGGTCTGGCGAAAAAGATGCTGCTCGTGCAG
GGTGAGGCGTACCGCCAGCAGTACGGTTTCAATTCGATCTTTCTATTGCCGGTCAATCTCTACGGCCCACGCGATAATTT
CGATTTAGAAACGTCGCACGTGATTCCTGCCCTGATCCGCAAATGTATTGAAGCCACCGAACGCGGTGACGATGAGATTG
TGGTTTGGGGTGACGGTTCACCAACCCGCGAGTTCATTTATGCCGCTGATGCTGCTGAAGGTATCTTGCTGGCGAGTGAG
CGTTACAACGATCCGGCACCGGTGAACATTGGCAGTAGTTACGAAATTAGCATTCGCGATCTGGTCACCTTGATCGCCGA
TCTGACCGGCTTTCGCGGGCGGATTGTCTGGGATACGACGAAGCCCAATGGTCAACCGCGTCGTAAGCTCGATGTGAGCC
GGGCGTGGGAGCGGTTTGGCTTCCGGGCCGAAACGACGTTTGCCGATGGTCTGCGGGCAACGATTGCATGGTATCGCAGC
CAGCGTGAGTCGCTTATGGCAATGCGAGCAGTTGGTGAGGCACACTGA

Upstream 100 bases:

>100_bases
GGCCAGTGTCTTGACGAACGGTGTGATCAAAGACGCACTTGATAGCCAGTACCGCAACGCTCAGTTCATCGTCCAGTAAG
CCCTGCTCTGTGAGGTTTCT

Downstream 100 bases:

>100_bases
TATGACAATGGTACATGGTTCGGCACCAGCAATTACCGATCTACCACCACCACCGGCTAACCGGTCGGGCTGGCCGTGGA
CGGTCGCAACGCCACCGGCT

Product: NAD-dependent epimerase/dehydratase

Products: GDP-4-dehydro-6-L-deoxygalactose; guanosine diphosphate fucose; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MHLNNHHWPDSYEFWQDKSVVVTGGAGFLGSYVVEKLHERGARRIVVPRSHQYDLRQLEAIRQLLADAQPDIVIHMAARV
GGIGANRDHPAEFFYDNLMMGVQLLHESWRFGVQKFVTIGTVCAYPKYTPVPFKEDDLWNGYPEETNAPYGLAKKMLLVQ
GEAYRQQYGFNSIFLLPVNLYGPRDNFDLETSHVIPALIRKCIEATERGDDEIVVWGDGSPTREFIYAADAAEGILLASE
RYNDPAPVNIGSSYEISIRDLVTLIADLTGFRGRIVWDTTKPNGQPRRKLDVSRAWERFGFRAETTFADGLRATIAWYRS
QRESLMAMRAVGEAH

Sequences:

>Translated_335_residues
MHLNNHHWPDSYEFWQDKSVVVTGGAGFLGSYVVEKLHERGARRIVVPRSHQYDLRQLEAIRQLLADAQPDIVIHMAARV
GGIGANRDHPAEFFYDNLMMGVQLLHESWRFGVQKFVTIGTVCAYPKYTPVPFKEDDLWNGYPEETNAPYGLAKKMLLVQ
GEAYRQQYGFNSIFLLPVNLYGPRDNFDLETSHVIPALIRKCIEATERGDDEIVVWGDGSPTREFIYAADAAEGILLASE
RYNDPAPVNIGSSYEISIRDLVTLIADLTGFRGRIVWDTTKPNGQPRRKLDVSRAWERFGFRAETTFADGLRATIAWYRS
QRESLMAMRAVGEAH
>Mature_335_residues
MHLNNHHWPDSYEFWQDKSVVVTGGAGFLGSYVVEKLHERGARRIVVPRSHQYDLRQLEAIRQLLADAQPDIVIHMAARV
GGIGANRDHPAEFFYDNLMMGVQLLHESWRFGVQKFVTIGTVCAYPKYTPVPFKEDDLWNGYPEETNAPYGLAKKMLLVQ
GEAYRQQYGFNSIFLLPVNLYGPRDNFDLETSHVIPALIRKCIEATERGDDEIVVWGDGSPTREFIYAADAAEGILLASE
RYNDPAPVNIGSSYEISIRDLVTLIADLTGFRGRIVWDTTKPNGQPRRKLDVSRAWERFGFRAETTFADGLRATIAWYRS
QRESLMAMRAVGEAH

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=310, Percent_Identity=33.5483870967742, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI7657641, Length=337, Percent_Identity=24.6290801186944, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI42516563, Length=322, Percent_Identity=23.9130434782609, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1788365, Length=315, Percent_Identity=35.2380952380952, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI48994969, Length=343, Percent_Identity=23.0320699708455, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786974, Length=331, Percent_Identity=22.6586102719033, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17554428, Length=312, Percent_Identity=33.0128205128205, Blast_Score=184, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI115532424, Length=339, Percent_Identity=24.188790560472, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922778, Length=311, Percent_Identity=31.1897106109325, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI21356223, Length=322, Percent_Identity=25.776397515528, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 1.1.1.271 [C]

Molecular weight: Translated: 38053; Mature: 38053

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLNNHHWPDSYEFWQDKSVVVTGGAGFLGSYVVEKLHERGARRIVVPRSHQYDLRQLEA
CCCCCCCCCCCCHHHCCCEEEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHH
IRQLLADAQPDIVIHMAARVGGIGANRDHPAEFFYDNLMMGVQLLHESWRFGVQKFVTIG
HHHHHCCCCCCEEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVCAYPKYTPVPFKEDDLWNGYPEETNAPYGLAKKMLLVQGEAYRQQYGFNSIFLLPVNL
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCEEEEEEEEE
YGPRDNFDLETSHVIPALIRKCIEATERGDDEIVVWGDGSPTREFIYAADAAEGILLASE
ECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHEEEECCCCCCEEEECC
RYNDPAPVNIGSSYEISIRDLVTLIADLTGFRGRIVWDTTKPNGQPRRKLDVSRAWERFG
CCCCCCCEECCCCCEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHC
FRAETTFADGLRATIAWYRSQRESLMAMRAVGEAH
CEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHLNNHHWPDSYEFWQDKSVVVTGGAGFLGSYVVEKLHERGARRIVVPRSHQYDLRQLEA
CCCCCCCCCCCCHHHCCCEEEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHH
IRQLLADAQPDIVIHMAARVGGIGANRDHPAEFFYDNLMMGVQLLHESWRFGVQKFVTIG
HHHHHCCCCCCEEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVCAYPKYTPVPFKEDDLWNGYPEETNAPYGLAKKMLLVQGEAYRQQYGFNSIFLLPVNL
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCEEEEEEEEE
YGPRDNFDLETSHVIPALIRKCIEATERGDDEIVVWGDGSPTREFIYAADAAEGILLASE
ECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHEEEECCCCCCEEEECC
RYNDPAPVNIGSSYEISIRDLVTLIADLTGFRGRIVWDTTKPNGQPRRKLDVSRAWERFG
CCCCCCCEECCCCCEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHC
FRAETTFADGLRATIAWYRSQRESLMAMRAVGEAH
CEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-4-dehydro-6-deoxy-D-mannose; GDP-4-dehydro-6-L-deoxygalactose; NADPH; Proton [C]

Specific reaction: GDP-4-dehydro-6-deoxy-D-mannose = GDP-4-dehydro-6-L-deoxygalactose GDP-4-dehydro-6-L-deoxygalactose + NADPH + Proton = guanosine diphosphate fucose + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]