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The map label for this gene is lacA [C]

Identifier: 222525531

GI number: 222525531

Start: 2884991

End: 2885680

Strand: Direct

Name: lacA [C]

Synonym: Chy400_2280

Alternate gene names: 222525531

Gene position: 2884991-2885680 (Clockwise)

Preceding gene: 222525530

Following gene: 222525532

Centisome position: 54.75

GC content: 59.86

Gene sequence:

>690_bases
ATGCGACAACGACTGGTACGGCTCTGGACTGACTGGTGGATGCGGCAGGCCGGCCCTGGGCGCTGGGGGAAAGTGGCGAC
ATGGCTGGCAGCGTGGCCGCACGCGCCATACAAAGCACGGGTCGCGCTCGCTCATCACTATCCACAGGGCTATGTTTCGC
CATCGGCCCAATTGAGTGGTCGTCGTATCCGCCGAGGGCGGCATGTCTTTATCGGCGACGGCGTTGTGATCTATCAGCGT
CAGGATGGCGGCCAGATTGATCTGGGTGATTACGTAGAACTCCACCGCGATACGATCATCGAATGTGGTCGTGGTGGTAG
TCTGCGAATCGGCGAACGAACCGGTATTCAGCCCCGCTGTCAACTATCGGCGTATATTGAACAGATTGTCATCGGGCGTG
GCTGCCAGATTGCACCACAATGTGCATTCTACCCCTACGACCACGGTACAGCACCCGGTCAGCCGATAGGGAGCCAGCCC
CTGACTAGCAAGGGGCCTATCGTGCTGGAAGATGATGTCTGGTTGGGTTACGGGGTTGTGGTGCTGTCGGGGGTCACGAT
TGGTAGCGGGACGGTGGTGGGAGCCGGGAGTGTTGTAACCCGCAGTCTGCCGGCAGGGGTGATTGCCGCCGGTATTCCGG
CGCGTATTGTACGTGAGCGAACTGAGCAGGATGTGGAACGTACGAGGTGA

Upstream 100 bases:

>100_bases
CGCCCGCCGATGAGGAACTGTTCTGGCAATACCATCGCGCGGGCATGCTGGCTGCCGGTTACGGAGATCGAGCGGCCTGA
TCATAGCGGGAGAAGCCGTT

Downstream 100 bases:

>100_bases
ACAATGGCAGCGACGGTAACGGTGGCAATCCCGGTTTACAAGCGACTAACTTACGTCGCCCAGGCCATCCGATCAGTAGC
TGATCAGGACTATCCGGCTA

Product: transferase hexapeptide repeat containing protein

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MRQRLVRLWTDWWMRQAGPGRWGKVATWLAAWPHAPYKARVALAHHYPQGYVSPSAQLSGRRIRRGRHVFIGDGVVIYQR
QDGGQIDLGDYVELHRDTIIECGRGGSLRIGERTGIQPRCQLSAYIEQIVIGRGCQIAPQCAFYPYDHGTAPGQPIGSQP
LTSKGPIVLEDDVWLGYGVVVLSGVTIGSGTVVGAGSVVTRSLPAGVIAAGIPARIVRERTEQDVERTR

Sequences:

>Translated_229_residues
MRQRLVRLWTDWWMRQAGPGRWGKVATWLAAWPHAPYKARVALAHHYPQGYVSPSAQLSGRRIRRGRHVFIGDGVVIYQR
QDGGQIDLGDYVELHRDTIIECGRGGSLRIGERTGIQPRCQLSAYIEQIVIGRGCQIAPQCAFYPYDHGTAPGQPIGSQP
LTSKGPIVLEDDVWLGYGVVVLSGVTIGSGTVVGAGSVVTRSLPAGVIAAGIPARIVRERTEQDVERTR
>Mature_229_residues
MRQRLVRLWTDWWMRQAGPGRWGKVATWLAAWPHAPYKARVALAHHYPQGYVSPSAQLSGRRIRRGRHVFIGDGVVIYQR
QDGGQIDLGDYVELHRDTIIECGRGGSLRIGERTGIQPRCQLSAYIEQIVIGRGCQIAPQCAFYPYDHGTAPGQPIGSQP
LTSKGPIVLEDDVWLGYGVVVLSGVTIGSGTVVGAGSVVTRSLPAGVIAAGIPARIVRERTEQDVERTR

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=101, Percent_Identity=37.6237623762376, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788345, Length=108, Percent_Identity=37.037037037037, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1786664, Length=133, Percent_Identity=30.0751879699248, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1788367, Length=69, Percent_Identity=50.7246376811594, Blast_Score=61, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322243, Length=106, Percent_Identity=38.6792452830189, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 25101; Mature: 25101

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQRLVRLWTDWWMRQAGPGRWGKVATWLAAWPHAPYKARVALAHHYPQGYVSPSAQLSG
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCC
RRIRRGRHVFIGDGVVIYQRQDGGQIDLGDYVELHRDTIIECGRGGSLRIGERTGIQPRC
HHHHCCCEEEEECCEEEEEECCCCEECCHHHHHHHHHHHEECCCCCCEEECCCCCCCCHH
QLSAYIEQIVIGRGCQIAPQCAFYPYDHGTAPGQPIGSQPLTSKGPIVLEDDVWLGYGVV
HHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHEEEE
VLSGVTIGSGTVVGAGSVVTRSLPAGVIAAGIPARIVRERTEQDVERTR
HHCCEEECCCCEEECCHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRQRLVRLWTDWWMRQAGPGRWGKVATWLAAWPHAPYKARVALAHHYPQGYVSPSAQLSG
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCC
RRIRRGRHVFIGDGVVIYQRQDGGQIDLGDYVELHRDTIIECGRGGSLRIGERTGIQPRC
HHHHCCCEEEEECCEEEEEECCCCEECCHHHHHHHHHHHEECCCCCCEEECCCCCCCCHH
QLSAYIEQIVIGRGCQIAPQCAFYPYDHGTAPGQPIGSQPLTSKGPIVLEDDVWLGYGVV
HHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHEEEE
VLSGVTIGSGTVVGAGSVVTRSLPAGVIAAGIPARIVRERTEQDVERTR
HHCCEEECCCCEEECCHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]