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The map label for this gene is tilS [H]

Identifier: 222525450

GI number: 222525450

Start: 2785006

End: 2786430

Strand: Direct

Name: tilS [H]

Synonym: Chy400_2198

Alternate gene names: 222525450

Gene position: 2785006-2786430 (Clockwise)

Preceding gene: 222525449

Following gene: 222525451

Centisome position: 52.86

GC content: 59.79

Gene sequence:

>1425_bases
ATGCTTGATCTATTGCGCCAGTTTTTACGTGCTGAGGGCGTCGAGCAGGCTTCTGCTCGCCTGCTGGTTGCTGTCTCTGG
CGGCAGCGACTCACTTGCCCTGCTGCACATGCTCTGCACATTGCACCGTGCTGGTGGCCCGACCCCAATTGTAGCGCATC
TCGATCACGGCCTGCGAGGTGCTGAATCTGCGGCAGATGCCGACTTCGTCGCTGCTACTGCGACGGCCTGGGGGGTACGG
GCAATTGTCGGACGTGCCCACTTGCCACCAGGAACAACACCTGCCGAGGCGCGAACTGCACGCTACACCTTTCTTGCCAC
AACCGCCCTCGCCGAACACGTCGATGCTGTGCTCGTTGCTCATCAGGCTGATGATCAGGCGGAAACGGTCTTGATGCACT
TACTCCGTGGCGCCGGGCCGGCTGGCTTGCGCGGTATGCGGGCGCGGGTTGAGTGGTCGGAATGGGCAGCAGCCCTGATT
GAGAGCGGACAGGCTCCGCCCCACGGCCCACCATTGTTGCGACCACTGCTAACGGTCAGTCGGTCTGAACTGGCGGCGTA
CTGCGCTGCTCATCAGTTAACCCCACGCGACGATCCAACAAACCGCAATCTCCATTACGTGCGGGCACGTATCAGGCATG
TGCTCTTGCCTCAGCTTGCAAGCGAGAATCCTCAGATCACTGCTGCCCTTACTCGTACTGCCCGTATCTGTGCTGAAGAT
TACGATTTTATTCAGACCATGCTGGCAGATCAGTGGCCGAACCTCATCGTTGAGCAGCAGTCTGACAAAGTGGTATTTGA
GCGTGCTGCCCTGATGAGTATCCATCCAGCCCTACGCCGCTATGCGTTGCGGCGTGCGATTGCTGAACTCGGTGCAGAGG
AACCGGGGTTAGCCCATCTCGATGCCGCACTGCATCTCATCGGAGGGCAGTCTGGTCGCTCGACGCAGATCGCTCCCCAG
GTTACACTGGTGTGCGATCAGCAGACAGTGACATTGCACTTGCCATCGGCATTACCGCCACCTGCCCCGCAGTTGACAAC
ACCTGTGACATTACCGGATCGGGGGATCGTGCCACTGGCGAATGACTGGCAGTGTATCGTGCAGGATCATCCCCCGGCGC
ATCCTGACCGCTGGTGGTTAGGACTGGCTCAGCGTCCGTCTGCTCCTTTGCAGTTACGACCACGGCGCCCCGGTGATCGG
ATGCGGCCTGCCGGTGCGCCGGGGCGGCGACGCTTACAAGATGTGTTCGTTGATTTGCGCGTGCCACGTATGCTGCGGGC
TGCCTGGCCGATTCTCACCACTGTCGATGATCAGATTCTCTGGGTCGTTGGCTTGCGAGTTGCTGCCGGCGTCGCTGCAT
CTGAGCCGAATCAGGCTACAATGTGGATTGGGATCGTTCACTCTGCCACAACAAGACAGGAGTAA

Upstream 100 bases:

>100_bases
CGGCTCATAATCGTTAGGTCGCGGGTTCGAATCCCGCCTCCGGCACCAGTCGCCGGTGAGCAACGCTCACCGGCGTTTCC
GTTCATCGTGCAATACCACC

Downstream 100 bases:

>100_bases
GCATGCGTCAGGACATTGATTACATCTTGATTGGGGAAGATCAGATCAAACAGCGGGTTGCGGAGCTGGCTGCTGAGATC
AGTGCCGATTATCGCGACCT

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MLDLLRQFLRAEGVEQASARLLVAVSGGSDSLALLHMLCTLHRAGGPTPIVAHLDHGLRGAESAADADFVAATATAWGVR
AIVGRAHLPPGTTPAEARTARYTFLATTALAEHVDAVLVAHQADDQAETVLMHLLRGAGPAGLRGMRARVEWSEWAAALI
ESGQAPPHGPPLLRPLLTVSRSELAAYCAAHQLTPRDDPTNRNLHYVRARIRHVLLPQLASENPQITAALTRTARICAED
YDFIQTMLADQWPNLIVEQQSDKVVFERAALMSIHPALRRYALRRAIAELGAEEPGLAHLDAALHLIGGQSGRSTQIAPQ
VTLVCDQQTVTLHLPSALPPPAPQLTTPVTLPDRGIVPLANDWQCIVQDHPPAHPDRWWLGLAQRPSAPLQLRPRRPGDR
MRPAGAPGRRRLQDVFVDLRVPRMLRAAWPILTTVDDQILWVVGLRVAAGVAASEPNQATMWIGIVHSATTRQE

Sequences:

>Translated_474_residues
MLDLLRQFLRAEGVEQASARLLVAVSGGSDSLALLHMLCTLHRAGGPTPIVAHLDHGLRGAESAADADFVAATATAWGVR
AIVGRAHLPPGTTPAEARTARYTFLATTALAEHVDAVLVAHQADDQAETVLMHLLRGAGPAGLRGMRARVEWSEWAAALI
ESGQAPPHGPPLLRPLLTVSRSELAAYCAAHQLTPRDDPTNRNLHYVRARIRHVLLPQLASENPQITAALTRTARICAED
YDFIQTMLADQWPNLIVEQQSDKVVFERAALMSIHPALRRYALRRAIAELGAEEPGLAHLDAALHLIGGQSGRSTQIAPQ
VTLVCDQQTVTLHLPSALPPPAPQLTTPVTLPDRGIVPLANDWQCIVQDHPPAHPDRWWLGLAQRPSAPLQLRPRRPGDR
MRPAGAPGRRRLQDVFVDLRVPRMLRAAWPILTTVDDQILWVVGLRVAAGVAASEPNQATMWIGIVHSATTRQE
>Mature_474_residues
MLDLLRQFLRAEGVEQASARLLVAVSGGSDSLALLHMLCTLHRAGGPTPIVAHLDHGLRGAESAADADFVAATATAWGVR
AIVGRAHLPPGTTPAEARTARYTFLATTALAEHVDAVLVAHQADDQAETVLMHLLRGAGPAGLRGMRARVEWSEWAAALI
ESGQAPPHGPPLLRPLLTVSRSELAAYCAAHQLTPRDDPTNRNLHYVRARIRHVLLPQLASENPQITAALTRTARICAED
YDFIQTMLADQWPNLIVEQQSDKVVFERAALMSIHPALRRYALRRAIAELGAEEPGLAHLDAALHLIGGQSGRSTQIAPQ
VTLVCDQQTVTLHLPSALPPPAPQLTTPVTLPDRGIVPLANDWQCIVQDHPPAHPDRWWLGLAQRPSAPLQLRPRRPGDR
MRPAGAPGRRRLQDVFVDLRVPRMLRAAWPILTTVDDQILWVVGLRVAAGVAASEPNQATMWIGIVHSATTRQE

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=241, Percent_Identity=34.8547717842324, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 51487; Mature: 51487

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLLRQFLRAEGVEQASARLLVAVSGGSDSLALLHMLCTLHRAGGPTPIVAHLDHGLRG
CHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
AESAADADFVAATATAWGVRAIVGRAHLPPGTTPAEARTARYTFLATTALAEHVDAVLVA
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEEHHHHHHHHHHHHHHEEEE
HQADDQAETVLMHLLRGAGPAGLRGMRARVEWSEWAAALIESGQAPPHGPPLLRPLLTVS
ECCCCHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
RSELAAYCAAHQLTPRDDPTNRNLHYVRARIRHVLLPQLASENPQITAALTRTARICAED
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHH
YDFIQTMLADQWPNLIVEQQSDKVVFERAALMSIHPALRRYALRRAIAELGAEEPGLAHL
HHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
DAALHLIGGQSGRSTQIAPQVTLVCDQQTVTLHLPSALPPPAPQLTTPVTLPDRGIVPLA
HHHHHHHCCCCCCCCCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCEECCCCCCCCCC
NDWQCIVQDHPPAHPDRWWLGLAQRPSAPLQLRPRRPGDRMRPAGAPGRRRLQDVFVDLR
CCCEEEECCCCCCCCCCEEEECCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
VPRMLRAAWPILTTVDDQILWVVGLRVAAGVAASEPNQATMWIGIVHSATTRQE
HHHHHHHHCCHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCC
>Mature Secondary Structure
MLDLLRQFLRAEGVEQASARLLVAVSGGSDSLALLHMLCTLHRAGGPTPIVAHLDHGLRG
CHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
AESAADADFVAATATAWGVRAIVGRAHLPPGTTPAEARTARYTFLATTALAEHVDAVLVA
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEEHHHHHHHHHHHHHHEEEE
HQADDQAETVLMHLLRGAGPAGLRGMRARVEWSEWAAALIESGQAPPHGPPLLRPLLTVS
ECCCCHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
RSELAAYCAAHQLTPRDDPTNRNLHYVRARIRHVLLPQLASENPQITAALTRTARICAED
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHH
YDFIQTMLADQWPNLIVEQQSDKVVFERAALMSIHPALRRYALRRAIAELGAEEPGLAHL
HHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
DAALHLIGGQSGRSTQIAPQVTLVCDQQTVTLHLPSALPPPAPQLTTPVTLPDRGIVPLA
HHHHHHHCCCCCCCCCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCEECCCCCCCCCC
NDWQCIVQDHPPAHPDRWWLGLAQRPSAPLQLRPRRPGDRMRPAGAPGRRRLQDVFVDLR
CCCEEEECCCCCCCCCCEEEECCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
VPRMLRAAWPILTTVDDQILWVVGLRVAAGVAASEPNQATMWIGIVHSATTRQE
HHHHHHHHCCHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA