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The map label for this gene is bhbA [H]

Identifier: 222525255

GI number: 222525255

Start: 2529888

End: 2531582

Strand: Reverse

Name: bhbA [H]

Synonym: Chy400_1995

Alternate gene names: 222525255

Gene position: 2531582-2529888 (Counterclockwise)

Preceding gene: 222525256

Following gene: 222525254

Centisome position: 48.05

GC content: 58.29

Gene sequence:

>1695_bases
ATGATGTCTGACATCGAGCAGCTCGCTGCCGCCCACGAACAATGGGAACAGCAGTGCCTCTGGCCAGCCCTGCGCCGCAC
GCCTGAACGTTCTGGCCCCTTTATGACGACCAGTAGTGCACCAATTGAGCGTCTCTACACCCCGCTTGATCTCACTCGTG
ATGGCGAAACCCTGACCGACCACTTCCTGCGCAACATCGCCTACCCCGGTCAGTATCCGTTCACGCGCGGCATTCATCCG
ACCGGCTATCGCGGCAAATTGTGGACGATGCGCATGTTTGCCGGCTTCGGTAGCGCCGAGGAGACAAATGCCCGTTTCAA
GTATCTGCTCGAGCAAGGGCAGACCGGATTGTCAATTGCGTTTGATATGCCCACGTTGTACGGGCGTGACACGGATCATC
CGCTGGTTGAGGGTGAATTTGGCAAGTGTGGGGTGGCTGTCTCGTCACTGGCCGATATGGAGATTTTGCTGGCCGATCTG
CCGCTGGATCAGGTCAGCACCTCAATGACCATCAATTCGCCGGCGGCGATGATTTGGGCCATGTATCTGGTGGTGGCCGA
AAAGCGCGGCATTCCGTGGAGCCAGTTGCGGGGTACGATTCAGAACGATATTCTGAAGGAGTACATTGCCCAGAACGAAT
ACATTTTCCCACCTGAACCGAGTATGCGGCTGGTGGTCGATACCATCGAATTTGCCGCTCGCCACGTACCACAATGGAAC
CCGATTAGCGTGAGTGGGTATCACATCCGCGAGGCTGGTAGCACTGCTGTGCAAGAGCTGGCGTTTACGCTGGCCGATGG
CTTTGCCTATGTCGAAGCGGCGCTGGAGCGGGGTCTCGATATTGATGAATTTGCCCCCCGGATCAGCTTCTTCTTCAATG
CCCACAATGACTTCTTCGAGGAGATTGCCAAGTATCGCGCTGCCCGGCGCATTTGGGCGCGGGCGATGCGTGAACGCTAC
GGTGCGAAGAATGAGCGCTCGTGGTGGCTGCGCTTCCACACCCAAACCGCCGGCTGCTCGCTCACTGCCCAGCAACCTGA
GATCAACATCGTCCGCACGGCGATCCAGGCGCTGGCAGCCGTGCTCGGTGGCACACAGAGCCTGCATACCAACTCGATGG
ACGAGGCGCTGGCGTTGCCGTCGGAAAAGGCGGTAACGATTGCTCTGCGCACGCAGCAGATTATTGCCTACGAGAGTGGT
GTTGCCAACACGGTTGATCCACTCGGCGGTAGCTATTTCGTCGAAGCGTTAACCGACCGCATGGAGCGTGAAGCTCAGCA
GATCTTTGACGCGATCAATGCCCAGGGTGGGGTGATTGCAGCGATCCGCAACGGCTACTATCACCGCGAGATTGCCGATG
CTGCCTATCGCTATCAGCAAGAGATCGACCGTGGGGAGCGCATTATTGTGGGAGTGAATGCGTTCCAGGATGATGAGCCG
CTGACGATTCCGATCTTGCAGATGGATCCGGAGGGCGAGAAGCGTCATCTGGAGCGACTGAATAGGGTGCGGCGTGAGCG
CGATCAGGCGCTGGTCGCTCGCCGGCTGGCAGAGTTGCGCGCCGCCGCTCAGGGGAACGAGAACATGATGCCGGCAATCC
TCGACTGTGTACGGGCGTATTGTACACTCGGCGAGATGTGTGATGTGCTGCGTGAAGTGTTCGGGGTCTATCAGCAAGAC
TCGGTGATCGTGTAG

Upstream 100 bases:

>100_bases
TGCGCTGGCGCGTTCGCTGATTGACGACTCCGTTGTACAATAGAAGTATACGCGCACAGAGTGCGCTTCGGCAATCTACC
ATTCTCAGTCAGGAGGGACG

Downstream 100 bases:

>100_bases
CAACGGCTTCATGGTCAGAGAGGGATTGTTTTCAGGGGCGCAGGGCATGCGCCCCTGTGATTTTTTCTGCACAGGGGCTG
TCTGTTGATAATATCCTATC

Product: methylmalonyl-CoA mutase large subunit

Products: NA

Alternate protein names: MCM [H]

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MMSDIEQLAAAHEQWEQQCLWPALRRTPERSGPFMTTSSAPIERLYTPLDLTRDGETLTDHFLRNIAYPGQYPFTRGIHP
TGYRGKLWTMRMFAGFGSAEETNARFKYLLEQGQTGLSIAFDMPTLYGRDTDHPLVEGEFGKCGVAVSSLADMEILLADL
PLDQVSTSMTINSPAAMIWAMYLVVAEKRGIPWSQLRGTIQNDILKEYIAQNEYIFPPEPSMRLVVDTIEFAARHVPQWN
PISVSGYHIREAGSTAVQELAFTLADGFAYVEAALERGLDIDEFAPRISFFFNAHNDFFEEIAKYRAARRIWARAMRERY
GAKNERSWWLRFHTQTAGCSLTAQQPEINIVRTAIQALAAVLGGTQSLHTNSMDEALALPSEKAVTIALRTQQIIAYESG
VANTVDPLGGSYFVEALTDRMEREAQQIFDAINAQGGVIAAIRNGYYHREIADAAYRYQQEIDRGERIIVGVNAFQDDEP
LTIPILQMDPEGEKRHLERLNRVRRERDQALVARRLAELRAAAQGNENMMPAILDCVRAYCTLGEMCDVLREVFGVYQQD
SVIV

Sequences:

>Translated_564_residues
MMSDIEQLAAAHEQWEQQCLWPALRRTPERSGPFMTTSSAPIERLYTPLDLTRDGETLTDHFLRNIAYPGQYPFTRGIHP
TGYRGKLWTMRMFAGFGSAEETNARFKYLLEQGQTGLSIAFDMPTLYGRDTDHPLVEGEFGKCGVAVSSLADMEILLADL
PLDQVSTSMTINSPAAMIWAMYLVVAEKRGIPWSQLRGTIQNDILKEYIAQNEYIFPPEPSMRLVVDTIEFAARHVPQWN
PISVSGYHIREAGSTAVQELAFTLADGFAYVEAALERGLDIDEFAPRISFFFNAHNDFFEEIAKYRAARRIWARAMRERY
GAKNERSWWLRFHTQTAGCSLTAQQPEINIVRTAIQALAAVLGGTQSLHTNSMDEALALPSEKAVTIALRTQQIIAYESG
VANTVDPLGGSYFVEALTDRMEREAQQIFDAINAQGGVIAAIRNGYYHREIADAAYRYQQEIDRGERIIVGVNAFQDDEP
LTIPILQMDPEGEKRHLERLNRVRRERDQALVARRLAELRAAAQGNENMMPAILDCVRAYCTLGEMCDVLREVFGVYQQD
SVIV
>Mature_564_residues
MMSDIEQLAAAHEQWEQQCLWPALRRTPERSGPFMTTSSAPIERLYTPLDLTRDGETLTDHFLRNIAYPGQYPFTRGIHP
TGYRGKLWTMRMFAGFGSAEETNARFKYLLEQGQTGLSIAFDMPTLYGRDTDHPLVEGEFGKCGVAVSSLADMEILLADL
PLDQVSTSMTINSPAAMIWAMYLVVAEKRGIPWSQLRGTIQNDILKEYIAQNEYIFPPEPSMRLVVDTIEFAARHVPQWN
PISVSGYHIREAGSTAVQELAFTLADGFAYVEAALERGLDIDEFAPRISFFFNAHNDFFEEIAKYRAARRIWARAMRERY
GAKNERSWWLRFHTQTAGCSLTAQQPEINIVRTAIQALAAVLGGTQSLHTNSMDEALALPSEKAVTIALRTQQIIAYESG
VANTVDPLGGSYFVEALTDRMEREAQQIFDAINAQGGVIAAIRNGYYHREIADAAYRYQQEIDRGERIIVGVNAFQDDEP
LTIPILQMDPEGEKRHLERLNRVRRERDQALVARRLAELRAAAQGNENMMPAILDCVRAYCTLGEMCDVLREVFGVYQQD
SVIV

Specific function: Catalyzes the interconversion of succinyl-CoA and methylmalonyl-CoA during the polyhydroxyalkanoate degradation pathway [H]

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=519, Percent_Identity=46.0500963391137, Blast_Score=474, Evalue=1e-134,
Organism=Escherichia coli, GI1789284, Length=523, Percent_Identity=45.5066921606119, Blast_Score=446, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI25144500, Length=520, Percent_Identity=45.1923076923077, Blast_Score=469, Evalue=1e-132,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 63612; Mature: 63612

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSDIEQLAAAHEQWEQQCLWPALRRTPERSGPFMTTSSAPIERLYTPLDLTRDGETLTD
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHCCCCCCCCCCCHHHH
HFLRNIAYPGQYPFTRGIHPTGYRGKLWTMRMFAGFGSAEETNARFKYLLEQGQTGLSIA
HHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEE
FDMPTLYGRDTDHPLVEGEFGKCGVAVSSLADMEILLADLPLDQVSTSMTINSPAAMIWA
EECCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCEEECCHHHHHHH
MYLVVAEKRGIPWSQLRGTIQNDILKEYIAQNEYIFPPEPSMRLVVDTIEFAARHVPQWN
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCEEHHHHHHHHHHHCCCCC
PISVSGYHIREAGSTAVQELAFTLADGFAYVEAALERGLDIDEFAPRISFFFNAHNDFFE
CEECCCEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEECCCHHHHH
EIAKYRAARRIWARAMRERYGAKNERSWWLRFHTQTAGCSLTAQQPEINIVRTAIQALAA
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEECCCCCHHHHHHHHHHHHH
VLGGTQSLHTNSMDEALALPSEKAVTIALRTQQIIAYESGVANTVDPLGGSYFVEALTDR
HHCCCCCCCCCCHHHHHCCCCCCEEEEEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
MEREAQQIFDAINAQGGVIAAIRNGYYHREIADAAYRYQQEIDRGERIIVGVNAFQDDEP
HHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
LTIPILQMDPEGEKRHLERLNRVRRERDQALVARRLAELRAAAQGNENMMPAILDCVRAY
CEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
CTLGEMCDVLREVFGVYQQDSVIV
HHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MMSDIEQLAAAHEQWEQQCLWPALRRTPERSGPFMTTSSAPIERLYTPLDLTRDGETLTD
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHCCCCCCCCCCCHHHH
HFLRNIAYPGQYPFTRGIHPTGYRGKLWTMRMFAGFGSAEETNARFKYLLEQGQTGLSIA
HHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEE
FDMPTLYGRDTDHPLVEGEFGKCGVAVSSLADMEILLADLPLDQVSTSMTINSPAAMIWA
EECCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCEEECCHHHHHHH
MYLVVAEKRGIPWSQLRGTIQNDILKEYIAQNEYIFPPEPSMRLVVDTIEFAARHVPQWN
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCEEHHHHHHHHHHHCCCCC
PISVSGYHIREAGSTAVQELAFTLADGFAYVEAALERGLDIDEFAPRISFFFNAHNDFFE
CEECCCEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEECCCHHHHH
EIAKYRAARRIWARAMRERYGAKNERSWWLRFHTQTAGCSLTAQQPEINIVRTAIQALAA
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEECCCCCHHHHHHHHHHHHH
VLGGTQSLHTNSMDEALALPSEKAVTIALRTQQIIAYESGVANTVDPLGGSYFVEALTDR
HHCCCCCCCCCCHHHHHCCCCCCEEEEEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
MEREAQQIFDAINAQGGVIAAIRNGYYHREIADAAYRYQQEIDRGERIIVGVNAFQDDEP
HHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
LTIPILQMDPEGEKRHLERLNRVRRERDQALVARRLAELRAAAQGNENMMPAILDCVRAY
CEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
CTLGEMCDVLREVFGVYQQDSVIV
HHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9889346; 11481431 [H]