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The map label for this gene is pcrA [H]

Identifier: 222525244

GI number: 222525244

Start: 2517408

End: 2519342

Strand: Direct

Name: pcrA [H]

Synonym: Chy400_1984

Alternate gene names: 222525244

Gene position: 2517408-2519342 (Clockwise)

Preceding gene: 222525242

Following gene: 222525258

Centisome position: 47.78

GC content: 58.97

Gene sequence:

>1935_bases
ATGGAACAACTCAACCTGCTGAATCCCGCCCAGCGCGCGGCTGTTACTGCCCCAATTGGGCCGATACTGGTCAAGGCCGG
GGCCGGTAGTGGCAAAACACGGGTCTTGACGCTGAGGATCGCCTATCTGATTACGCACTACGGGGTAGCGCCGCAGCAGA
TTCTGGCGGTTACTTTTACCAATAAGGCGGCACGCGAGATGCGTGAGCGGTTAAGCAATCTGCCAGGGCTGCGCATTCGT
GGTTTGACCAGCGGTACTTTTCATGCCATCTGCACACGTATCCTGCGCGAGTCGATTGAGGGTCGGCTGAAAGGATATAC
CTCTTCGTTCTCGATCTATGCCGGCGAGGAGCAATTGCAACTGGCGGCTGAGGCATTGGCGGGCGTAGGCGAGACACCAC
CCCGGTTGATCGAAGCCGATGAGGTGTTGCGGCTGATTTCACGGGCTAAAAGCCGGATGTTGACCCCACGCCTGATGGCA
CGGTTCGCCACCGACCCGCTTGATCGCTTTATCGCTGCCTGTTACCGGCGCTATCAGCGGGCACTCGAACAGGCGAATGC
CCTCGATTTCGATGATCTGATCCTGACCGCGTATCGCCTGCTGAGCGATGATCCCGATTTGCTGGCAACGTATCAGCAGC
GCTGGCAACACGTGCTGGTCGACGAGTACCAGGATACCGATCCGAGTCAGCACGCGCTGATTGAGTTGCTCACCCGGCCA
ACGGCGCAGCGTCCGCGCTCGCTCTTCGTGGTTGGCGATGCAATGCAGAGTATCTACGGCTTTCGCAACGCCGATCACAG
CATTATCAACCGCTTTGCTACCGATTTTCCCGATGCTCGTGTGATCGAGTTGACAACGAACTACCGTTCGCGCCAGCCTA
TTCTCGATGCGGCTTATGCAATTATTCGCCACAGCCGCAGCGTGGCACCGATGGAATTGCGTGCTGCGGCACGGAATGTT
GATGAACGTTGTGTGTTGATCAGCGAAGCCAGAGATAGTCGTGATGAGGCCGATCAGGTAGCTCGTTCGATTGCCGATCT
GCAACGGCAAGGGCGACGGTTGCGCGATATTGCCGTGCTCTACCGCACCCGTCATATGAGCCGACCGTTCGAGCAAGCCT
TGCGCCATGCCCGGATTCCGTACCAGGTGCGCGGTGGAGTGAGTTTCTTTGATCGGGCGGTGGTGCGTGATGCCCTGGCC
TATCTTCGCTGTATTGCCAATCCGGCGGATAACCTCAGTTTGACGCGCATTGCGAATGTGCCGGCGCGAGGGTTAGGCGG
GCAGGCGCTGGCAACCATTGCCGCCTATGCGGCAGCCCAATCGTTGCCGATCAGTACAGCTCTTGGCCATGCCCGGAATA
TTCCCGGTCTGAGTCCACGGGCGGTCGAGGGTGCCCATCGGCTCTTCGCATTGTTGCAACGCTGGTGGCGGTTGGCCGAA
AGCACCATGCCGCCGGATCACGTGCTGGCCGATGTGCTGGAGCAGAGTGGGTATATGGCGGCATTAGCTGAACGTTTCGA
CGCCGAGGAGCTGGCCGAGGCGCGTGCCCATTTGCAGGAGTTGCTGCGGGCAGCGGAAGAGCACACTGATCTGCGTTCGT
TCCTGCAAGAAGTTGCGCTGATGACGAATACAGACGATGAAGATGATGAGCGTGATCGGGTTCAGCTCTTGACGATCCAC
GCCGCCAAGGGGTTGGAGTGGCCATTTGTGTTTGTGGTGGGCTTAGAGGAGGGAACGCTGCCGCACGAACGGAGTATCGG
CGATCCGGCGGCTATCGAAGAAGAACGGCGCTTATGCTATGTGGCGCTGACCCGTGCCGCCGAACGGCTCTATCTCTCGT
GGACGGCAAGCCGGCAGCGTGGTCAGCGTCTGAAACCGTCGCGTTTTCTCGATGAGATCATTGCATTTGGACGAGAACGT
GCGCGAGGGCGATGA

Upstream 100 bases:

>100_bases
GGGGTAGCGGTCGCATTGCTGCACGTTACCCACTGCTGATCGACATACATCATTCAGCAAAGCATTATTGTCTAACAGCT
TCTAGCAAAACTGGCACGAT

Downstream 100 bases:

>100_bases
GGGAGCGAGAAGTCATCTCATTCATGGATTAGGCAAAGTGCGCAGAGTTGCAAGGCAGATGCAGCGACTCCCGCCCCAGC
GGGTTCAGGGGTGGACAGAA

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 644; Mature: 644

Protein sequence:

>644_residues
MEQLNLLNPAQRAAVTAPIGPILVKAGAGSGKTRVLTLRIAYLITHYGVAPQQILAVTFTNKAAREMRERLSNLPGLRIR
GLTSGTFHAICTRILRESIEGRLKGYTSSFSIYAGEEQLQLAAEALAGVGETPPRLIEADEVLRLISRAKSRMLTPRLMA
RFATDPLDRFIAACYRRYQRALEQANALDFDDLILTAYRLLSDDPDLLATYQQRWQHVLVDEYQDTDPSQHALIELLTRP
TAQRPRSLFVVGDAMQSIYGFRNADHSIINRFATDFPDARVIELTTNYRSRQPILDAAYAIIRHSRSVAPMELRAAARNV
DERCVLISEARDSRDEADQVARSIADLQRQGRRLRDIAVLYRTRHMSRPFEQALRHARIPYQVRGGVSFFDRAVVRDALA
YLRCIANPADNLSLTRIANVPARGLGGQALATIAAYAAAQSLPISTALGHARNIPGLSPRAVEGAHRLFALLQRWWRLAE
STMPPDHVLADVLEQSGYMAALAERFDAEELAEARAHLQELLRAAEEHTDLRSFLQEVALMTNTDDEDDERDRVQLLTIH
AAKGLEWPFVFVVGLEEGTLPHERSIGDPAAIEEERRLCYVALTRAAERLYLSWTASRQRGQRLKPSRFLDEIIAFGRER
ARGR

Sequences:

>Translated_644_residues
MEQLNLLNPAQRAAVTAPIGPILVKAGAGSGKTRVLTLRIAYLITHYGVAPQQILAVTFTNKAAREMRERLSNLPGLRIR
GLTSGTFHAICTRILRESIEGRLKGYTSSFSIYAGEEQLQLAAEALAGVGETPPRLIEADEVLRLISRAKSRMLTPRLMA
RFATDPLDRFIAACYRRYQRALEQANALDFDDLILTAYRLLSDDPDLLATYQQRWQHVLVDEYQDTDPSQHALIELLTRP
TAQRPRSLFVVGDAMQSIYGFRNADHSIINRFATDFPDARVIELTTNYRSRQPILDAAYAIIRHSRSVAPMELRAAARNV
DERCVLISEARDSRDEADQVARSIADLQRQGRRLRDIAVLYRTRHMSRPFEQALRHARIPYQVRGGVSFFDRAVVRDALA
YLRCIANPADNLSLTRIANVPARGLGGQALATIAAYAAAQSLPISTALGHARNIPGLSPRAVEGAHRLFALLQRWWRLAE
STMPPDHVLADVLEQSGYMAALAERFDAEELAEARAHLQELLRAAEEHTDLRSFLQEVALMTNTDDEDDERDRVQLLTIH
AAKGLEWPFVFVVGLEEGTLPHERSIGDPAAIEEERRLCYVALTRAAERLYLSWTASRQRGQRLKPSRFLDEIIAFGRER
ARGR
>Mature_644_residues
MEQLNLLNPAQRAAVTAPIGPILVKAGAGSGKTRVLTLRIAYLITHYGVAPQQILAVTFTNKAAREMRERLSNLPGLRIR
GLTSGTFHAICTRILRESIEGRLKGYTSSFSIYAGEEQLQLAAEALAGVGETPPRLIEADEVLRLISRAKSRMLTPRLMA
RFATDPLDRFIAACYRRYQRALEQANALDFDDLILTAYRLLSDDPDLLATYQQRWQHVLVDEYQDTDPSQHALIELLTRP
TAQRPRSLFVVGDAMQSIYGFRNADHSIINRFATDFPDARVIELTTNYRSRQPILDAAYAIIRHSRSVAPMELRAAARNV
DERCVLISEARDSRDEADQVARSIADLQRQGRRLRDIAVLYRTRHMSRPFEQALRHARIPYQVRGGVSFFDRAVVRDALA
YLRCIANPADNLSLTRIANVPARGLGGQALATIAAYAAAQSLPISTALGHARNIPGLSPRAVEGAHRLFALLQRWWRLAE
STMPPDHVLADVLEQSGYMAALAERFDAEELAEARAHLQELLRAAEEHTDLRSFLQEVALMTNTDDEDDERDRVQLLTIH
AAKGLEWPFVFVVGLEEGTLPHERSIGDPAAIEEERRLCYVALTRAAERLYLSWTASRQRGQRLKPSRFLDEIIAFGRER
ARGR

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=649, Percent_Identity=37.1340523882897, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI48994965, Length=644, Percent_Identity=38.5093167701863, Blast_Score=346, Evalue=3e-96,
Organism=Escherichia coli, GI1787196, Length=332, Percent_Identity=30.421686746988, Blast_Score=119, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6322369, Length=461, Percent_Identity=32.7548806941432, Blast_Score=174, Evalue=4e-44,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 72362; Mature: 72362

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQLNLLNPAQRAAVTAPIGPILVKAGAGSGKTRVLTLRIAYLITHYGVAPQQILAVTFT
CCCCCCCCHHHHHEECCCCCCEEEEECCCCCCCEEEHHHHHHHHHHCCCCHHHEEEEEEC
NKAAREMRERLSNLPGLRIRGLTSGTFHAICTRILRESIEGRLKGYTSSFSIYAGEEQLQ
CHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHH
LAAEALAGVGETPPRLIEADEVLRLISRAKSRMLTPRLMARFATDPLDRFIAACYRRYQR
HHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHH
ALEQANALDFDDLILTAYRLLSDDPDLLATYQQRWQHVLVDEYQDTDPSQHALIELLTRP
HHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
TAQRPRSLFVVGDAMQSIYGFRNADHSIINRFATDFPDARVIELTTNYRSRQPILDAAYA
CCCCCCEEEEECHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHH
IIRHSRSVAPMELRAAARNVDERCVLISEARDSRDEADQVARSIADLQRQGRRLRDIAVL
HHHHCCCCCHHHHHHHHCCCCHHEEEEHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHH
YRTRHMSRPFEQALRHARIPYQVRGGVSFFDRAVVRDALAYLRCIANPADNLSLTRIANV
HHHHHCCCHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHCCCC
PARGLGGQALATIAAYAAAQSLPISTALGHARNIPGLSPRAVEGAHRLFALLQRWWRLAE
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
STMPPDHVLADVLEQSGYMAALAERFDAEELAEARAHLQELLRAAEEHTDLRSFLQEVAL
CCCCHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTNTDDEDDERDRVQLLTIHAAKGLEWPFVFVVGLEEGTLPHERSIGDPAAIEEERRLCY
HCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
VALTRAAERLYLSWTASRQRGQRLKPSRFLDEIIAFGRERARGR
HHHHHHHHHHHHEEHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEQLNLLNPAQRAAVTAPIGPILVKAGAGSGKTRVLTLRIAYLITHYGVAPQQILAVTFT
CCCCCCCCHHHHHEECCCCCCEEEEECCCCCCCEEEHHHHHHHHHHCCCCHHHEEEEEEC
NKAAREMRERLSNLPGLRIRGLTSGTFHAICTRILRESIEGRLKGYTSSFSIYAGEEQLQ
CHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHH
LAAEALAGVGETPPRLIEADEVLRLISRAKSRMLTPRLMARFATDPLDRFIAACYRRYQR
HHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHH
ALEQANALDFDDLILTAYRLLSDDPDLLATYQQRWQHVLVDEYQDTDPSQHALIELLTRP
HHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
TAQRPRSLFVVGDAMQSIYGFRNADHSIINRFATDFPDARVIELTTNYRSRQPILDAAYA
CCCCCCEEEEECHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHH
IIRHSRSVAPMELRAAARNVDERCVLISEARDSRDEADQVARSIADLQRQGRRLRDIAVL
HHHHCCCCCHHHHHHHHCCCCHHEEEEHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHH
YRTRHMSRPFEQALRHARIPYQVRGGVSFFDRAVVRDALAYLRCIANPADNLSLTRIANV
HHHHHCCCHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHCCCC
PARGLGGQALATIAAYAAAQSLPISTALGHARNIPGLSPRAVEGAHRLFALLQRWWRLAE
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
STMPPDHVLADVLEQSGYMAALAERFDAEELAEARAHLQELLRAAEEHTDLRSFLQEVAL
CCCCHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTNTDDEDDERDRVQLLTIHAAKGLEWPFVFVVGLEEGTLPHERSIGDPAAIEEERRLCY
HCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
VALTRAAERLYLSWTASRQRGQRLKPSRFLDEIIAFGRERARGR
HHHHHHHHHHHHEEHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]