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The map label for this gene is pimB [H]

Identifier: 222525208

GI number: 222525208

Start: 2472445

End: 2473497

Strand: Reverse

Name: pimB [H]

Synonym: Chy400_1948

Alternate gene names: 222525208

Gene position: 2473497-2472445 (Counterclockwise)

Preceding gene: 222525209

Following gene: 222525207

Centisome position: 46.94

GC content: 60.59

Gene sequence:

>1053_bases
ATGCGCATCGCTTTTCTTGATTCATGGCTTCAGCACGTGGCTGAAGGGAGTGGTACCGCCGCCGCGATTGGCGGGCTGGG
ACGCGCTCTGCGTGCCCATGGTCATCATGTGGCCCGGATTGCGCCACCGGTGGCCTGGCCGGCGAACCTGACGTTGCGGC
GCTTGTTGTTCAATCTGTACGTGCCGGCGCTGCTGCGCACGCTGCCCTACGACCTGATCGTGGGGGTGGATATTGATGGG
GTGTTTGTCGCCGGGCGCGTCACCACCCCCTATGTGTGCAGTATTAAGGGTGTGATCGCTGAAGAGCTGCAACACGAACG
TGGTAATGTCCGCCGCTTGCTCTGGTTGCTGTCGCGCCTCGAACGGATGAATGCCCGTCAGTCACCACTGATTATTACCA
CGAGCAACTATTGTCGGCAGAGTATTCACCGCCATTACGGTGTGCCGCCGGAGCGGATTCGGCTGGTGCCGGAAGGGATT
GATTTGACGCAATGGCCGGCACCTGTGGCACGTGATGGTGGGCAGACCATTCTCTGTGTGGCGCGGCAATACCCGCGCAA
GCATATTGCCGACCTGATCCGGGCATTTGCGCAGGTGCGGCAGCGGCTACCTGACGCCCGTCTGGTCATTATTGGTGATG
GCCCGGAACACGCCAATCTGAAAACTCTGGCCCACACGCTCGGTTTGGGAGCGTCCTGTCAGCTTCTCGGTGCGTTACCG
TCTGATGATGAAGTGCGGTCGTGGTACTACCGGGCCGATCTCTTCTGTCTGCCGAGTGTGCAGGAGGGCTTTGGGATTGT
GTTTCTGGAGGCGATGGCTGCCAGTTTGCCGATTGTGGCGACCACGGCAGCCGCGATCCCGGAAGTTGTACCGCACGGTA
TGGCCGGTTTGCTGGTGCCGCCCGCTGACGTAGCGGCCCTGGCCGACGCGCTGACCACTCTGCTTGGTGATGTGTCGTTG
CGTCGGCGCTACGGATCGTTTGGGCGCGATTATGTGGCTCAGTTTGACTGGATGCGGGTTGCCGAACAATTTCTCACCGT
CACACGTGGTTGA

Upstream 100 bases:

>100_bases
ACACGCATTGCCGTGATGTGACAGGCGTTCTGTGATATACTCCTAAGCGGAATGCTTTGACTACGCTTGCCTGCCCTGAT
AGCAAGGGCGAGAAACATCA

Downstream 100 bases:

>100_bases
AACCAATGTCAATTGAAGTTCGCGGTCTGCGCAAGTACTATCAGGTGCATCGCAAAGAAGCCGGCTTGATGGGATCAATC
CGGGCGTTTGTCCGTCGTCA

Product: group 1 glycosyl transferase

Products: NA

Alternate protein names: Alpha-D-mannose-alpha-(1-6)-phosphatidylmyo-inositol-mannosyltransferase; Alpha-mannosyltransferase; Alpha-manT; Guanosine diphosphomannose-phosphatidyl-inositol alpha-mannosyltransferase; Phosphatidylinositol alpha-mannosyltransferase; PI alpha-mannosyltransferase [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MRIAFLDSWLQHVAEGSGTAAAIGGLGRALRAHGHHVARIAPPVAWPANLTLRRLLFNLYVPALLRTLPYDLIVGVDIDG
VFVAGRVTTPYVCSIKGVIAEELQHERGNVRRLLWLLSRLERMNARQSPLIITTSNYCRQSIHRHYGVPPERIRLVPEGI
DLTQWPAPVARDGGQTILCVARQYPRKHIADLIRAFAQVRQRLPDARLVIIGDGPEHANLKTLAHTLGLGASCQLLGALP
SDDEVRSWYYRADLFCLPSVQEGFGIVFLEAMAASLPIVATTAAAIPEVVPHGMAGLLVPPADVAALADALTTLLGDVSL
RRRYGSFGRDYVAQFDWMRVAEQFLTVTRG

Sequences:

>Translated_350_residues
MRIAFLDSWLQHVAEGSGTAAAIGGLGRALRAHGHHVARIAPPVAWPANLTLRRLLFNLYVPALLRTLPYDLIVGVDIDG
VFVAGRVTTPYVCSIKGVIAEELQHERGNVRRLLWLLSRLERMNARQSPLIITTSNYCRQSIHRHYGVPPERIRLVPEGI
DLTQWPAPVARDGGQTILCVARQYPRKHIADLIRAFAQVRQRLPDARLVIIGDGPEHANLKTLAHTLGLGASCQLLGALP
SDDEVRSWYYRADLFCLPSVQEGFGIVFLEAMAASLPIVATTAAAIPEVVPHGMAGLLVPPADVAALADALTTLLGDVSL
RRRYGSFGRDYVAQFDWMRVAEQFLTVTRG
>Mature_350_residues
MRIAFLDSWLQHVAEGSGTAAAIGGLGRALRAHGHHVARIAPPVAWPANLTLRRLLFNLYVPALLRTLPYDLIVGVDIDG
VFVAGRVTTPYVCSIKGVIAEELQHERGNVRRLLWLLSRLERMNARQSPLIITTSNYCRQSIHRHYGVPPERIRLVPEGI
DLTQWPAPVARDGGQTILCVARQYPRKHIADLIRAFAQVRQRLPDARLVIIGDGPEHANLKTLAHTLGLGASCQLLGALP
SDDEVRSWYYRADLFCLPSVQEGFGIVFLEAMAASLPIVATTAAAIPEVVPHGMAGLLVPPADVAALADALTTLLGDVSL
RRRYGSFGRDYVAQFDWMRVAEQFLTVTRG

Specific function: Catalyzes the addition of a mannose residue from GDP-D- mannose to the position 6 of a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1) to generate phosphatidyl-myo-inositol bearing alpha-1,2- and alpha-1,6-linked mannose resid

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Homo sapiens, GI11863130, Length=197, Percent_Identity=28.9340101522843, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788357, Length=168, Percent_Identity=33.9285714285714, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI9755344, Length=223, Percent_Identity=26.0089686098655, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: =2.4.1.57 [H]

Molecular weight: Translated: 38430; Mature: 38430

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAFLDSWLQHVAEGSGTAAAIGGLGRALRAHGHHVARIAPPVAWPANLTLRRLLFNLY
CEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHH
VPALLRTLPYDLIVGVDIDGVFVAGRVTTPYVCSIKGVIAEELQHERGNVRRLLWLLSRL
HHHHHHHCCHHEEEECCCCCEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERMNARQSPLIITTSNYCRQSIHRHYGVPPERIRLVPEGIDLTQWPAPVARDGGQTILCV
HHCCCCCCCEEEECCHHHHHHHHHHCCCCHHHEEEECCCCCCCCCCCCHHCCCCCEEEEE
ARQYPRKHIADLIRAFAQVRQRLPDARLVIIGDGPEHANLKTLAHTLGLGASCQLLGALP
EHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCCHHEEECCC
SDDEVRSWYYRADLFCLPSVQEGFGIVFLEAMAASLPIVATTAAAIPEVVPHGMAGLLVP
CCHHHHHHHHHHCEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEC
PADVAALADALTTLLGDVSLRRRYGSFGRDYVAQFDWMRVAEQFLTVTRG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRIAFLDSWLQHVAEGSGTAAAIGGLGRALRAHGHHVARIAPPVAWPANLTLRRLLFNLY
CEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHH
VPALLRTLPYDLIVGVDIDGVFVAGRVTTPYVCSIKGVIAEELQHERGNVRRLLWLLSRL
HHHHHHHCCHHEEEECCCCCEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERMNARQSPLIITTSNYCRQSIHRHYGVPPERIRLVPEGIDLTQWPAPVARDGGQTILCV
HHCCCCCCCEEEECCHHHHHHHHHHCCCCHHHEEEECCCCCCCCCCCCHHCCCCCEEEEE
ARQYPRKHIADLIRAFAQVRQRLPDARLVIIGDGPEHANLKTLAHTLGLGASCQLLGALP
EHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCCHHEEECCC
SDDEVRSWYYRADLFCLPSVQEGFGIVFLEAMAASLPIVATTAAAIPEVVPHGMAGLLVP
CCHHHHHHHHHHCEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEC
PADVAALADALTTLLGDVSLRRRYGSFGRDYVAQFDWMRVAEQFLTVTRG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]