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The map label for this gene is potD-A [H]

Identifier: 222525177

GI number: 222525177

Start: 2421009

End: 2422061

Strand: Direct

Name: potD-A [H]

Synonym: Chy400_1915

Alternate gene names: 222525177

Gene position: 2421009-2422061 (Clockwise)

Preceding gene: 222525176

Following gene: 222525178

Centisome position: 45.95

GC content: 52.04

Gene sequence:

>1053_bases
ATGATACGACCACTAACACTATGTTTGTTGTTACTCACGTTGGTTGCCTGTAGCACATCAGCACCTGCGCCTGTAGCAGA
CAGATTGGTCATTACGGGTTGGGCCGGCTATATGCCGCAGGCGATTCTGGATGCCTTCCAGGCTGAGACTGGGGTTACGG
TTGAGTACCGTATCTATGAAGAACAGCCCGCTGCTATCGAGCAAATGCGGGCCGGCGCCGATTACGACCTGGTGGTCATG
GGGAGTTTTTTTGTACCGCAACTGATCAAGGATGGTCTGCTTGCACCGATTGATTATCGTCAGATTCCCAAATTCCGCTA
TGTCAGTGCGAATTTTCGCGATCTGAGTTACGACCCCGGCGGGAAACATTCAATTGTCTATCAGTGGGGGGTTGGAGGAT
TGGTGGTACGGCCTGATTTACTTGAGCGACCGGTGACGCGCTGGGCCGATCTGTGGGATCCGTCTTTGGACGGTAAGATT
GCCATGTGGGTGACAGAAGATGACTTGTTTGCCATTGCCCTCAAATCAATCGGCCAACCCCTGGGTACGACCGATCCGGC
AGTACTAGACCAGGCTGCCGAACGCATTCGTGAACTACTCCCTCGGATTGTTGCCCTCGATCCCACGATGCCTAATGCCG
CAACATTGTTGGCTGACGGCACCTATCCGATTGTGTATGGCTGGGCGTTTGATGCACTGGAAGCACAGTCGCTCAATCCG
GCGGTTGAGTTTGTATTGCCGGAAGAAGGGGCCATACTCTGGATCGATACATTTGTTGTACCGGCCAGTAGCACTCGCAA
GTCACTGGCGTGGCAGTTTATCGATTTTGTCTTGCGTCCAGAAATGAGTGCCCTGATTACCAACGAGATCTTTGTTGCTA
CTGCGAATGAATTTGCGTCGCAATTCATCGATCCAGCATTGCGTGATCATCCCTGGGTCTTTCCCAGTGGAACCGATCTG
ATGAACGCCGAATATCTGGAAGAACTACCACCGGACATCCTCTCTTATCGCCATCAACTCTGGCAAGAATTAGAGAGTAT
CACTACGAGGTGA

Upstream 100 bases:

>100_bases
GTCTTACAGAACGCTGAAATAGAATTGTCACTTGACCCGACAACACAAGCAATACATAATGAAATATACGCAGCCTTCAT
ATCAGCAAAACCGTAAATGT

Downstream 100 bases:

>100_bases
TCTGTGCACTCCCTGCGTCGTCGCCTACATCACTTGTTTACTCACTTTAACACCAGCTTGCAAGCCAAACTGGTGCTCTT
TACGAGCCTCATGCTAACGG

Product: family 1 extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MIRPLTLCLLLLTLVACSTSAPAPVADRLVITGWAGYMPQAILDAFQAETGVTVEYRIYEEQPAAIEQMRAGADYDLVVM
GSFFVPQLIKDGLLAPIDYRQIPKFRYVSANFRDLSYDPGGKHSIVYQWGVGGLVVRPDLLERPVTRWADLWDPSLDGKI
AMWVTEDDLFAIALKSIGQPLGTTDPAVLDQAAERIRELLPRIVALDPTMPNAATLLADGTYPIVYGWAFDALEAQSLNP
AVEFVLPEEGAILWIDTFVVPASSTRKSLAWQFIDFVLRPEMSALITNEIFVATANEFASQFIDPALRDHPWVFPSGTDL
MNAEYLEELPPDILSYRHQLWQELESITTR

Sequences:

>Translated_350_residues
MIRPLTLCLLLLTLVACSTSAPAPVADRLVITGWAGYMPQAILDAFQAETGVTVEYRIYEEQPAAIEQMRAGADYDLVVM
GSFFVPQLIKDGLLAPIDYRQIPKFRYVSANFRDLSYDPGGKHSIVYQWGVGGLVVRPDLLERPVTRWADLWDPSLDGKI
AMWVTEDDLFAIALKSIGQPLGTTDPAVLDQAAERIRELLPRIVALDPTMPNAATLLADGTYPIVYGWAFDALEAQSLNP
AVEFVLPEEGAILWIDTFVVPASSTRKSLAWQFIDFVLRPEMSALITNEIFVATANEFASQFIDPALRDHPWVFPSGTDL
MNAEYLEELPPDILSYRHQLWQELESITTR
>Mature_350_residues
MIRPLTLCLLLLTLVACSTSAPAPVADRLVITGWAGYMPQAILDAFQAETGVTVEYRIYEEQPAAIEQMRAGADYDLVVM
GSFFVPQLIKDGLLAPIDYRQIPKFRYVSANFRDLSYDPGGKHSIVYQWGVGGLVVRPDLLERPVTRWADLWDPSLDGKI
AMWVTEDDLFAIALKSIGQPLGTTDPAVLDQAAERIRELLPRIVALDPTMPNAATLLADGTYPIVYGWAFDALEAQSLNP
AVEFVLPEEGAILWIDTFVVPASSTRKSLAWQFIDFVLRPEMSALITNEIFVATANEFASQFIDPALRDHPWVFPSGTDL
MNAEYLEELPPDILSYRHQLWQELESITTR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=314, Percent_Identity=27.7070063694268, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1787078, Length=359, Percent_Identity=25.0696378830084, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39047; Mature: 39047

Theoretical pI: Translated: 4.15; Mature: 4.15

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRPLTLCLLLLTLVACSTSAPAPVADRLVITGWAGYMPQAILDAFQAETGVTVEYRIYE
CCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCEEEEEEEC
EQPAAIEQMRAGADYDLVVMGSFFVPQLIKDGLLAPIDYRQIPKFRYVSANFRDLSYDPG
CCCHHHHHHHCCCCCEEEEECHHHHHHHHHCCCCCCCCHHHCCCEEEEECCCCCCCCCCC
GKHSIVYQWGVGGLVVRPDLLERPVTRWADLWDPSLDGKIAMWVTEDDLFAIALKSIGQP
CCEEEEEEECCCCEEECCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHCCCC
LGTTDPAVLDQAAERIRELLPRIVALDPTMPNAATLLADGTYPIVYGWAFDALEAQSLNP
CCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEECCCCEEEEEEEHHHHHHCCCCC
AVEFVLPEEGAILWIDTFVVPASSTRKSLAWQFIDFVLRPEMSALITNEIFVATANEFAS
EEEEEECCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHCCEEEEEHHHHHH
QFIDPALRDHPWVFPSGTDLMNAEYLEELPPDILSYRHQLWQELESITTR
HHHCHHHHCCCEECCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIRPLTLCLLLLTLVACSTSAPAPVADRLVITGWAGYMPQAILDAFQAETGVTVEYRIYE
CCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCEEEEEEEC
EQPAAIEQMRAGADYDLVVMGSFFVPQLIKDGLLAPIDYRQIPKFRYVSANFRDLSYDPG
CCCHHHHHHHCCCCCEEEEECHHHHHHHHHCCCCCCCCHHHCCCEEEEECCCCCCCCCCC
GKHSIVYQWGVGGLVVRPDLLERPVTRWADLWDPSLDGKIAMWVTEDDLFAIALKSIGQP
CCEEEEEEECCCCEEECCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHCCCC
LGTTDPAVLDQAAERIRELLPRIVALDPTMPNAATLLADGTYPIVYGWAFDALEAQSLNP
CCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEECCCCEEEEEEEHHHHHHCCCCC
AVEFVLPEEGAILWIDTFVVPASSTRKSLAWQFIDFVLRPEMSALITNEIFVATANEFAS
EEEEEECCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHCCEEEEEHHHHHH
QFIDPALRDHPWVFPSGTDLMNAEYLEELPPDILSYRHQLWQELESITTR
HHHCHHHHCCCEECCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]