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The map label for this gene is potD-A [H]

Identifier: 222525176

GI number: 222525176

Start: 2419952

End: 2421004

Strand: Direct

Name: potD-A [H]

Synonym: Chy400_1914

Alternate gene names: 222525176

Gene position: 2419952-2421004 (Clockwise)

Preceding gene: 222525175

Following gene: 222525177

Centisome position: 45.93

GC content: 50.81

Gene sequence:

>1053_bases
ATGCGAATACTGTTTATCATGGCGAGCCTGTTGCTCGCGGCGTGCTCGCCTGGGGCTATCATCAACCCGGTCTCAACTCC
TCCCAAACTGACGGTGACCGGTTGGGTTGGGTATATGCCTCAAGCCATCATTGATGCATTCAGTGCTGAGACTGGAGTGA
GCGTCGAATATATTGGATACGACACACCCGAAGAGGCATTGATCGCCCTGGAACAGGGTGCTAACTACGATTTGATGATC
ATGGTCAATTCCTTAGTACCCGATCTGATTGCTGCCGGTAAACTTGCCCCCTTAAACTACGCGAACATTCCCAATGCCCG
CAATATCAGCCCTAGCTTTCGCGATCTGATCTTTGATCCTGGGGGACGCCATTCAATCGTCTATCAGTGGGGCACAACTG
GCTTACTGGTACGTACCGATCTGGTTGAACAACCAATCACACGCTGGGCCGATCTGTGGAAACCAGACCTCGCAGGGAAG
ATCGTGCTCTGGCCAATTCCCCGCGATACCATCAATGTGTTATTAAAGTCGCTTGGCTATTCGATCAACACTACTGATCC
CGGCCAACTTGCTGTAGCTCGTGAACGGGCTGCCGAACTGGCTCACCGGGTAACCCTGACCCAGGAAGGTGATGAGGTTG
TGACGCCCTATCTCCTGTCTGACGAGCACGTGGTTGCGCTCGGTTGGACGTATGACGCATTTGATGCTCAGGCGCAGAAC
GAGAATATCGCATACATCATACCTGCTGAGGGTACCATTCTCTGGTTTGACAACTTTGTCATTCCGACCACCAGCTCAAA
CAGGGCATTGGCCGAACGCTTTATCGATTTTGTGTTACGACCCGAGATGAGTGCACTTGTAACGAACGAACTGGCGGTAG
CAACCGGTAACGAAGTAGCCTATCCACTGATTGATCCTGCTCTCCGTAACAACCCGGCTATTTTTCCATCCGGTGATGTC
TTACAGAACGCTGAAATAGAATTGTCACTTGACCCGACAACACAAGCAATACATAATGAAATATACGCAGCCTTCATATC
AGCAAAACCGTAA

Upstream 100 bases:

>100_bases
TTTTTACAGCAACATCTGTTGCTGGTAACATTGTGGTAATGCCAGGACGGTATATATCGAATGTATATGCAGTCGTCACG
TGGGGAAAGTTCATACCGCT

Downstream 100 bases:

>100_bases
ATGTATGATACGACCACTAACACTATGTTTGTTGTTACTCACGTTGGTTGCCTGTAGCACATCAGCACCTGCGCCTGTAG
CAGACAGATTGGTCATTACG

Product: family 1 extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MRILFIMASLLLAACSPGAIINPVSTPPKLTVTGWVGYMPQAIIDAFSAETGVSVEYIGYDTPEEALIALEQGANYDLMI
MVNSLVPDLIAAGKLAPLNYANIPNARNISPSFRDLIFDPGGRHSIVYQWGTTGLLVRTDLVEQPITRWADLWKPDLAGK
IVLWPIPRDTINVLLKSLGYSINTTDPGQLAVARERAAELAHRVTLTQEGDEVVTPYLLSDEHVVALGWTYDAFDAQAQN
ENIAYIIPAEGTILWFDNFVIPTTSSNRALAERFIDFVLRPEMSALVTNELAVATGNEVAYPLIDPALRNNPAIFPSGDV
LQNAEIELSLDPTTQAIHNEIYAAFISAKP

Sequences:

>Translated_350_residues
MRILFIMASLLLAACSPGAIINPVSTPPKLTVTGWVGYMPQAIIDAFSAETGVSVEYIGYDTPEEALIALEQGANYDLMI
MVNSLVPDLIAAGKLAPLNYANIPNARNISPSFRDLIFDPGGRHSIVYQWGTTGLLVRTDLVEQPITRWADLWKPDLAGK
IVLWPIPRDTINVLLKSLGYSINTTDPGQLAVARERAAELAHRVTLTQEGDEVVTPYLLSDEHVVALGWTYDAFDAQAQN
ENIAYIIPAEGTILWFDNFVIPTTSSNRALAERFIDFVLRPEMSALVTNELAVATGNEVAYPLIDPALRNNPAIFPSGDV
LQNAEIELSLDPTTQAIHNEIYAAFISAKP
>Mature_350_residues
MRILFIMASLLLAACSPGAIINPVSTPPKLTVTGWVGYMPQAIIDAFSAETGVSVEYIGYDTPEEALIALEQGANYDLMI
MVNSLVPDLIAAGKLAPLNYANIPNARNISPSFRDLIFDPGGRHSIVYQWGTTGLLVRTDLVEQPITRWADLWKPDLAGK
IVLWPIPRDTINVLLKSLGYSINTTDPGQLAVARERAAELAHRVTLTQEGDEVVTPYLLSDEHVVALGWTYDAFDAQAQN
ENIAYIIPAEGTILWFDNFVIPTTSSNRALAERFIDFVLRPEMSALVTNELAVATGNEVAYPLIDPALRNNPAIFPSGDV
LQNAEIELSLDPTTQAIHNEIYAAFISAKP

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=325, Percent_Identity=29.8461538461538, Blast_Score=161, Evalue=7e-41,
Organism=Escherichia coli, GI1787078, Length=341, Percent_Identity=25.5131964809384, Blast_Score=117, Evalue=8e-28,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38230; Mature: 38230

Theoretical pI: Translated: 4.19; Mature: 4.19

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILFIMASLLLAACSPGAIINPVSTPPKLTVTGWVGYMPQAIIDAFSAETGVSVEYIGY
CCHHHHHHHHHHHHCCCCCEECCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCEEEEECC
DTPEEALIALEQGANYDLMIMVNSLVPDLIAAGKLAPLNYANIPNARNISPSFRDLIFDP
CCCHHHEEEEECCCCEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHEECC
GGRHSIVYQWGTTGLLVRTDLVEQPITRWADLWKPDLAGKIVLWPIPRDTINVLLKSLGY
CCCEEEEEEECCCEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHCC
SINTTDPGQLAVARERAAELAHRVTLTQEGDEVVTPYLLSDEHVVALGWTYDAFDAQAQN
EECCCCCCHHHHHHHHHHHHHHHEEEECCCCCEECCEEECCCEEEEEEEEECCCCCCCCC
ENIAYIIPAEGTILWFDNFVIPTTSSNRALAERFIDFVLRPEMSALVTNELAVATGNEVA
CCEEEEEECCCEEEEECCEEEECCCCCHHHHHHHHHHHHCCCHHHHHHCCEEEECCCCEE
YPLIDPALRNNPAIFPSGDVLQNAEIELSLDPTTQAIHNEIYAAFISAKP
ECCCCHHHCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHEEEEEECCC
>Mature Secondary Structure
MRILFIMASLLLAACSPGAIINPVSTPPKLTVTGWVGYMPQAIIDAFSAETGVSVEYIGY
CCHHHHHHHHHHHHCCCCCEECCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCEEEEECC
DTPEEALIALEQGANYDLMIMVNSLVPDLIAAGKLAPLNYANIPNARNISPSFRDLIFDP
CCCHHHEEEEECCCCEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHEECC
GGRHSIVYQWGTTGLLVRTDLVEQPITRWADLWKPDLAGKIVLWPIPRDTINVLLKSLGY
CCCEEEEEEECCCEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHCC
SINTTDPGQLAVARERAAELAHRVTLTQEGDEVVTPYLLSDEHVVALGWTYDAFDAQAQN
EECCCCCCHHHHHHHHHHHHHHHEEEECCCCCEECCEEECCCEEEEEEEEECCCCCCCCC
ENIAYIIPAEGTILWFDNFVIPTTSSNRALAERFIDFVLRPEMSALVTNELAVATGNEVA
CCEEEEEECCCEEEEECCEEEECCCCCHHHHHHHHHHHHCCCHHHHHHCCEEEECCCCEE
YPLIDPALRNNPAIFPSGDVLQNAEIELSLDPTTQAIHNEIYAAFISAKP
ECCCCHHHCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]