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The map label for this gene is maeB [C]

Identifier: 222525016

GI number: 222525016

Start: 2213252

End: 2214670

Strand: Reverse

Name: maeB [C]

Synonym: Chy400_1751

Alternate gene names: 222525016

Gene position: 2214670-2213252 (Counterclockwise)

Preceding gene: 222525026

Following gene: 222525007

Centisome position: 42.03

GC content: 57.43

Gene sequence:

>1419_bases
ATGTCCGTTGGGGTGGCTTACACGCTGACGTTGCGCTGCCAGATTGAAAATCGTCCTGGGATGCTGGGGCGGTTGACCAC
CTTGATCGGCGAAGTCGGCGGTGACATCGGTGCGATTGACATTGTGCGCGCTGAACGGAACATTCTGGTTCGCGATATTA
CGGTGCGCGTACAAGACGAGCAACACGGGGAGCAACTGGTAGCAGCAATTAACACGTTGCAGAACATCAAAGTGCTTCAG
GTAAGTGACCGTGTCTTTCTTACCCACCTCGGCGGCAAGCTGGCCACGCAGAGTCGCGTACCTCTGAAGACGCGCGATGA
TCTTTCGCTGGCCTACACTCCCGGTGTGGCCCGTGTCTGTCGGGCAATTGCCGATGATCCGGAGAAGGTGTACGCACTGA
CCTGGAAAGGCAACAGTGTGGCCGTCGTGAGCGATGGTTCGGCCATTTTGGGGCTGGGCAACCTGGGGCCGGAAGCGGCG
ATGCCGGTGATGGAGGGCAAAGCGATTCTGTTTAAAGAATTGGCGAACATCGATGCGGTGCCGATCTGTCTGCGCTCGCA
AGACCCTGATGTGATTGTGCAGACGGTTGAGCAGATCGCGCCGAGTTTTGGTGGCATTAACCTCGAAGATATTGCTGCTC
CAAATTGTTTCATTGTCGAGGGTCGGCTCGAAGAGAGTCTCGATATGCCGGTGATGCACGATGACCAGCACGGGACAGCG
GTGGTGGTGTTGGCCGCGCTGCGCAATGCGGTACGGATTGTCGGCAAGCGACTGGCAGATGTGCGTGTGGTTGTAAATGG
GATCGGGGCAGCCGGTACGGCGATCATTCGCACGCTGATCGAAGCCGGGGTTGGCGAAATTACTGCGGTGGATCGGTTTG
GGATCCTGGTCGAAGGTGATCATGCCCGTCAGACCCCGATGCAGCGGATTATTGCTTCACAGACGAACCGGGAACGACGG
CGCGGTGATTTAGCGGTGGCGTTGCGCGGTGCCGATGTCTTTATTGGGGTCTCGAAGGGCAATATCCTGACGCCCGACCA
TATTCGGGTGATGAGTAGTGATCCGATTGTCTTTGCGCTGGCCAATCCGATCCCCGAAGGCGATCCTGATATGTTGCGTC
AGTATGCACGTGTGGTTGCAACTGGCCGCAGTGATCAGCCAAATCAGATTAACAATGTGCTCAGCTTTCCCGGTATCTTC
CGTGGTGCGCTCGATGTACACGCAAGGCGTATTACCTCGGCCATGCGGCTCGCTGCCGCCGAAGCACTGGCGACGGTGAT
TCCACCGGAAGAGATGAATGAAGATTACATCGTGCCTAGTGTGTTTAACCGGCAGGTTGTGCCGGCCATTGCTGCGGCTG
TGGCACAGGCGGCGATTGCTGAAGGGGTGGCGCGGCGAACGCATATGCCGGGTGATTAG

Upstream 100 bases:

>100_bases
ACAAATCAAGACTTGTGGTACTATACCATGATAAGGCGATGATGCCCGTCGTGGTGACGGGCATTTTTGTGTGCTTATTT
CAGAAAACGAGGTGAGGGCT

Downstream 100 bases:

>100_bases
CGGGTTGAACGGTGCGACACGCGACCGGTATCGAGCATGGGCGTGCGGCAGCACGGCTGCCGCACGTCAACTTACGCGCC
ACGATCATCGTTGGGTGTAG

Product: Malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSVGVAYTLTLRCQIENRPGMLGRLTTLIGEVGGDIGAIDIVRAERNILVRDITVRVQDEQHGEQLVAAINTLQNIKVLQ
VSDRVFLTHLGGKLATQSRVPLKTRDDLSLAYTPGVARVCRAIADDPEKVYALTWKGNSVAVVSDGSAILGLGNLGPEAA
MPVMEGKAILFKELANIDAVPICLRSQDPDVIVQTVEQIAPSFGGINLEDIAAPNCFIVEGRLEESLDMPVMHDDQHGTA
VVVLAALRNAVRIVGKRLADVRVVVNGIGAAGTAIIRTLIEAGVGEITAVDRFGILVEGDHARQTPMQRIIASQTNRERR
RGDLAVALRGADVFIGVSKGNILTPDHIRVMSSDPIVFALANPIPEGDPDMLRQYARVVATGRSDQPNQINNVLSFPGIF
RGALDVHARRITSAMRLAAAEALATVIPPEEMNEDYIVPSVFNRQVVPAIAAAVAQAAIAEGVARRTHMPGD

Sequences:

>Translated_472_residues
MSVGVAYTLTLRCQIENRPGMLGRLTTLIGEVGGDIGAIDIVRAERNILVRDITVRVQDEQHGEQLVAAINTLQNIKVLQ
VSDRVFLTHLGGKLATQSRVPLKTRDDLSLAYTPGVARVCRAIADDPEKVYALTWKGNSVAVVSDGSAILGLGNLGPEAA
MPVMEGKAILFKELANIDAVPICLRSQDPDVIVQTVEQIAPSFGGINLEDIAAPNCFIVEGRLEESLDMPVMHDDQHGTA
VVVLAALRNAVRIVGKRLADVRVVVNGIGAAGTAIIRTLIEAGVGEITAVDRFGILVEGDHARQTPMQRIIASQTNRERR
RGDLAVALRGADVFIGVSKGNILTPDHIRVMSSDPIVFALANPIPEGDPDMLRQYARVVATGRSDQPNQINNVLSFPGIF
RGALDVHARRITSAMRLAAAEALATVIPPEEMNEDYIVPSVFNRQVVPAIAAAVAQAAIAEGVARRTHMPGD
>Mature_471_residues
SVGVAYTLTLRCQIENRPGMLGRLTTLIGEVGGDIGAIDIVRAERNILVRDITVRVQDEQHGEQLVAAINTLQNIKVLQV
SDRVFLTHLGGKLATQSRVPLKTRDDLSLAYTPGVARVCRAIADDPEKVYALTWKGNSVAVVSDGSAILGLGNLGPEAAM
PVMEGKAILFKELANIDAVPICLRSQDPDVIVQTVEQIAPSFGGINLEDIAAPNCFIVEGRLEESLDMPVMHDDQHGTAV
VVLAALRNAVRIVGKRLADVRVVVNGIGAAGTAIIRTLIEAGVGEITAVDRFGILVEGDHARQTPMQRIIASQTNRERRR
GDLAVALRGADVFIGVSKGNILTPDHIRVMSSDPIVFALANPIPEGDPDMLRQYARVVATGRSDQPNQINNVLSFPGIFR
GALDVHARRITSAMRLAAAEALATVIPPEEMNEDYIVPSVFNRQVVPAIAAAVAQAAIAEGVARRTHMPGD

Specific function: In addition to the NAD-dependent oxidative decarboxylation of L-malate, the enzyme catalyzes the decarboxylation of oxaloacetate [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505145, Length=402, Percent_Identity=27.8606965174129, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI4505143, Length=384, Percent_Identity=24.4791666666667, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI62420882, Length=403, Percent_Identity=24.8138957816377, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI62420880, Length=403, Percent_Identity=24.8138957816377, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI239049447, Length=403, Percent_Identity=24.8138957816377, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI270265879, Length=328, Percent_Identity=27.7439024390244, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1788806, Length=393, Percent_Identity=43.0025445292621, Blast_Score=311, Evalue=7e-86,
Organism=Escherichia coli, GI87081919, Length=405, Percent_Identity=27.9012345679012, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17537199, Length=450, Percent_Identity=23.5555555555556, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322823, Length=315, Percent_Identity=28.2539682539683, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI78707242, Length=392, Percent_Identity=27.2959183673469, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI78707238, Length=392, Percent_Identity=27.2959183673469, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI281362674, Length=392, Percent_Identity=26.2755102040816, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI281362672, Length=392, Percent_Identity=26.2755102040816, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21356279, Length=392, Percent_Identity=26.2755102040816, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI78707240, Length=389, Percent_Identity=26.7352185089974, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 50513; Mature: 50382

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVGVAYTLTLRCQIENRPGMLGRLTTLIGEVGGDIGAIDIVRAERNILVRDITVRVQDE
CCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEEEECCH
QHGEQLVAAINTLQNIKVLQVSDRVFLTHLGGKLATQSRVPLKTRDDLSLAYTPGVARVC
HHHHHHHHHHHHHHCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCHHHHH
RAIADDPEKVYALTWKGNSVAVVSDGSAILGLGNLGPEAAMPVMEGKAILFKELANIDAV
HHHCCCCCCEEEEEECCCEEEEEECCCEEEEECCCCCHHCCCHHCCCHHHHHHHCCCCCE
PICLRSQDPDVIVQTVEQIAPSFGGINLEDIAAPNCFIVEGRLEESLDMPVMHDDQHGTA
EEEECCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEECCHHHCCCCCCEECCCCCHH
VVVLAALRNAVRIVGKRLADVRVVVNGIGAAGTAIIRTLIEAGVGEITAVDRFGILVEGD
HHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEEEEEECC
HARQTPMQRIIASQTNRERRRGDLAVALRGADVFIGVSKGNILTPDHIRVMSSDPIVFAL
CCHHHHHHHHHHHHCCHHHHCCCEEEEEECCEEEEEECCCCEECCCCEEEECCCCEEEEE
ANPIPEGDPDMLRQYARVVATGRSDQPNQINNVLSFPGIFRGALDVHARRITSAMRLAAA
ECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
EALATVIPPEEMNEDYIVPSVFNRQVVPAIAAAVAQAAIAEGVARRTHMPGD
HHHHHCCCHHHCCCCEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SVGVAYTLTLRCQIENRPGMLGRLTTLIGEVGGDIGAIDIVRAERNILVRDITVRVQDE
CCCEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEEEECCH
QHGEQLVAAINTLQNIKVLQVSDRVFLTHLGGKLATQSRVPLKTRDDLSLAYTPGVARVC
HHHHHHHHHHHHHHCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCHHHHH
RAIADDPEKVYALTWKGNSVAVVSDGSAILGLGNLGPEAAMPVMEGKAILFKELANIDAV
HHHCCCCCCEEEEEECCCEEEEEECCCEEEEECCCCCHHCCCHHCCCHHHHHHHCCCCCE
PICLRSQDPDVIVQTVEQIAPSFGGINLEDIAAPNCFIVEGRLEESLDMPVMHDDQHGTA
EEEECCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEECCHHHCCCCCCEECCCCCHH
VVVLAALRNAVRIVGKRLADVRVVVNGIGAAGTAIIRTLIEAGVGEITAVDRFGILVEGD
HHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEEEEEECC
HARQTPMQRIIASQTNRERRRGDLAVALRGADVFIGVSKGNILTPDHIRVMSSDPIVFAL
CCHHHHHHHHHHHHCCHHHHCCCEEEEEECCEEEEEECCCCEECCCCEEEECCCCEEEEE
ANPIPEGDPDMLRQYARVVATGRSDQPNQINNVLSFPGIFRGALDVHARRITSAMRLAAA
ECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
EALATVIPPEEMNEDYIVPSVFNRQVVPAIAAAVAQAAIAEGVARRTHMPGD
HHHHHCCCHHHCCCCEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644282 [H]