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The map label for this gene is tcmA [H]

Identifier: 222525005

GI number: 222525005

Start: 2196837

End: 2198273

Strand: Direct

Name: tcmA [H]

Synonym: Chy400_1740

Alternate gene names: 222525005

Gene position: 2196837-2198273 (Clockwise)

Preceding gene: 222525004

Following gene: 222525006

Centisome position: 41.69

GC content: 58.46

Gene sequence:

>1437_bases
GTGAACGAAGGGCGCAAATGGTGGGCATTAGCAGCGGTCGGCACCGGTGTGCTGGTTTCGACCATTGATGGCTCGATTGT
CAATATTGCTCTGAACACCCTGGTACAGGCGTTTGCCAGCAATCTGAATGTTGTCGAATGGGTGGTCTTGGGCTACTTAC
TGACCCTGGTCTGCACACTCTTGATCATGGGTCGTCTGGGAGATATGTATGGCAAACGCCGGATGTATCTGATCGGCTTT
GCCCTCTTTACCATCGCTTCGTTGCTCTGCGCCACCGCGCCGTCGATTGCAGCCCTGATCGGCTTTCGGGTGCTACAGGG
AATTGGGGCCGCAATGATTCAAGCCGTCGGCCCGGCATTGCTGGTAACTGCCTTCCCTGCCCGTGAACGTGGCATGGCGC
TGGGAGCGATTGGCTCCTTCGTCGCCGCCGGTATTCTAATCGGGCCGGCCCTGGGTGGCGTGCTGTTGCAGTACGTCGGC
TGGGAAGCGATCTTCTTCGTCAACTTGCCGATTGGTGTGCTGGGCTGGTGGCTCACCATCCGCTCAATCGCACCCGACCA
CACGACGCGCCCCGGTCAGCGGTTTGATCTGATCGGAGCGGTATTGATGGCTGTCACCCTGTTTTGCCTCCTGCTGGGGC
TGACCGAAGGGCCATTGTGGGGATGGGGTGATAGTCGCGTGATCGGCCTCTTTGCAGCCTCGCTGATCGGTGGAATACTG
TTTGTCTGGTGGGAATGGCGTCATCCGCAGCCAATGCTGCAATTGCGCATGTTCCGCCGACTGGCCTTCTCGCTCAATCT
GCTGGCCGCGCTGATCCTCTTTCTTGGTATTTCCTTCAACCTGCTGCTGACGCCACTCTTCTTTCAACTGGTATACCAGT
TTGATCTCCAGCGCACCGGCTTGATGCTCATGGCGCTCCCCATTGCCCTCTCGCTCACCTCGCCGATCAGCGGACGGCTT
TCAGACCGGTTTGGGCCGCGAGTGTTGACTATCGTCGGATTGCTGGTGACCGGATGTGCCTTGCTGGGCCTGAGTACGAC
CACACCGGTCACACCACCGCTGCAAATGTTGAGTTTTCTGATATTACTGGGATTTGGAGTAGGTTTGTTCCAAAGCCCGA
ATAACAGTACAGTGTTAGGCAACGCCCCGCCAGAGGCATTGGGGGTGGCGAGTGGTTTACTGGCGGTCGTCCGCACTGTC
GGCCAAACCGGTGGCATTGCTCTGGCCGGTGCGATATGGGCATCGCAGATCTTTGCGTTGAACGGCGGCCCGGTGACTCC
CATCACTGCGGCACCGCCACCGATTCTGGCCGGCGGCTACGATATAGCAATGCGCGTTGCCGCGTTGCTTGCTGCAATCG
CGATTGTGCCATCGTTGATCGGCGGCTATGCACTTCAACGTCAAATGGCACCGGCCAGACCGCCGGTTGCCGATTAA

Upstream 100 bases:

>100_bases
CATCCAGCCGGGCACAACACTAAAGGCGAAACGTATCGTCATTCCTGCCATTGTGCGGTAGAATGCACCCGGTAACAATG
ATACGACGACGAGGTGGCGT

Downstream 100 bases:

>100_bases
TATACGTCGATGCAATCTTCACACCTGACACCCTTACCCCTCAACCGGATGCTTCATAACAGAGCGAATCTGGGATGAGG
TGAGGGTAGGTAAACACGGG

Product: EmrB/QacA subfamily drug resistance transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MNEGRKWWALAAVGTGVLVSTIDGSIVNIALNTLVQAFASNLNVVEWVVLGYLLTLVCTLLIMGRLGDMYGKRRMYLIGF
ALFTIASLLCATAPSIAALIGFRVLQGIGAAMIQAVGPALLVTAFPARERGMALGAIGSFVAAGILIGPALGGVLLQYVG
WEAIFFVNLPIGVLGWWLTIRSIAPDHTTRPGQRFDLIGAVLMAVTLFCLLLGLTEGPLWGWGDSRVIGLFAASLIGGIL
FVWWEWRHPQPMLQLRMFRRLAFSLNLLAALILFLGISFNLLLTPLFFQLVYQFDLQRTGLMLMALPIALSLTSPISGRL
SDRFGPRVLTIVGLLVTGCALLGLSTTTPVTPPLQMLSFLILLGFGVGLFQSPNNSTVLGNAPPEALGVASGLLAVVRTV
GQTGGIALAGAIWASQIFALNGGPVTPITAAPPPILAGGYDIAMRVAALLAAIAIVPSLIGGYALQRQMAPARPPVAD

Sequences:

>Translated_478_residues
MNEGRKWWALAAVGTGVLVSTIDGSIVNIALNTLVQAFASNLNVVEWVVLGYLLTLVCTLLIMGRLGDMYGKRRMYLIGF
ALFTIASLLCATAPSIAALIGFRVLQGIGAAMIQAVGPALLVTAFPARERGMALGAIGSFVAAGILIGPALGGVLLQYVG
WEAIFFVNLPIGVLGWWLTIRSIAPDHTTRPGQRFDLIGAVLMAVTLFCLLLGLTEGPLWGWGDSRVIGLFAASLIGGIL
FVWWEWRHPQPMLQLRMFRRLAFSLNLLAALILFLGISFNLLLTPLFFQLVYQFDLQRTGLMLMALPIALSLTSPISGRL
SDRFGPRVLTIVGLLVTGCALLGLSTTTPVTPPLQMLSFLILLGFGVGLFQSPNNSTVLGNAPPEALGVASGLLAVVRTV
GQTGGIALAGAIWASQIFALNGGPVTPITAAPPPILAGGYDIAMRVAALLAAIAIVPSLIGGYALQRQMAPARPPVAD
>Mature_478_residues
MNEGRKWWALAAVGTGVLVSTIDGSIVNIALNTLVQAFASNLNVVEWVVLGYLLTLVCTLLIMGRLGDMYGKRRMYLIGF
ALFTIASLLCATAPSIAALIGFRVLQGIGAAMIQAVGPALLVTAFPARERGMALGAIGSFVAAGILIGPALGGVLLQYVG
WEAIFFVNLPIGVLGWWLTIRSIAPDHTTRPGQRFDLIGAVLMAVTLFCLLLGLTEGPLWGWGDSRVIGLFAASLIGGIL
FVWWEWRHPQPMLQLRMFRRLAFSLNLLAALILFLGISFNLLLTPLFFQLVYQFDLQRTGLMLMALPIALSLTSPISGRL
SDRFGPRVLTIVGLLVTGCALLGLSTTTPVTPPLQMLSFLILLGFGVGLFQSPNNSTVLGNAPPEALGVASGLLAVVRTV
GQTGGIALAGAIWASQIFALNGGPVTPITAAPPPILAGGYDIAMRVAALLAAIAIVPSLIGGYALQRQMAPARPPVAD

Specific function: Resistance to tetracenomycin C by an active tetracenomycin C efflux system which is probably energized by transmembrane electrochemical gradients [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=427, Percent_Identity=32.3185011709602, Blast_Score=195, Evalue=7e-51,
Organism=Escherichia coli, GI1790195, Length=399, Percent_Identity=27.5689223057644, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1789042, Length=412, Percent_Identity=26.2135922330097, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1788710, Length=417, Percent_Identity=24.220623501199, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1788392, Length=436, Percent_Identity=28.8990825688073, Blast_Score=111, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6323735, Length=318, Percent_Identity=24.5283018867925, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322958, Length=412, Percent_Identity=24.5145631067961, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321663, Length=358, Percent_Identity=24.3016759776536, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324264, Length=203, Percent_Identity=31.0344827586207, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=132, Percent_Identity=31.8181818181818, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324954, Length=392, Percent_Identity=23.469387755102, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50444; Mature: 50444

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEGRKWWALAAVGTGVLVSTIDGSIVNIALNTLVQAFASNLNVVEWVVLGYLLTLVCTL
CCCCCEEEEEEHHCCCCEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LIMGRLGDMYGKRRMYLIGFALFTIASLLCATAPSIAALIGFRVLQGIGAAMIQAVGPAL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHH
LVTAFPARERGMALGAIGSFVAAGILIGPALGGVLLQYVGWEAIFFVNLPIGVLGWWLTI
HHEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHH
RSIAPDHTTRPGQRFDLIGAVLMAVTLFCLLLGLTEGPLWGWGDSRVIGLFAASLIGGIL
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
FVWWEWRHPQPMLQLRMFRRLAFSLNLLAALILFLGISFNLLLTPLFFQLVYQFDLQRTG
HHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMLMALPIALSLTSPISGRLSDRFGPRVLTIVGLLVTGCALLGLSTTTPVTPPLQMLSFL
HHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
ILLGFGVGLFQSPNNSTVLGNAPPEALGVASGLLAVVRTVGQTGGIALAGAIWASQIFAL
HHHHCCCHHEECCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEE
NGGPVTPITAAPPPILAGGYDIAMRVAALLAAIAIVPSLIGGYALQRQMAPARPPVAD
CCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNEGRKWWALAAVGTGVLVSTIDGSIVNIALNTLVQAFASNLNVVEWVVLGYLLTLVCTL
CCCCCEEEEEEHHCCCCEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LIMGRLGDMYGKRRMYLIGFALFTIASLLCATAPSIAALIGFRVLQGIGAAMIQAVGPAL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHH
LVTAFPARERGMALGAIGSFVAAGILIGPALGGVLLQYVGWEAIFFVNLPIGVLGWWLTI
HHEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHH
RSIAPDHTTRPGQRFDLIGAVLMAVTLFCLLLGLTEGPLWGWGDSRVIGLFAASLIGGIL
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
FVWWEWRHPQPMLQLRMFRRLAFSLNLLAALILFLGISFNLLLTPLFFQLVYQFDLQRTG
HHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMLMALPIALSLTSPISGRLSDRFGPRVLTIVGLLVTGCALLGLSTTTPVTPPLQMLSFL
HHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
ILLGFGVGLFQSPNNSTVLGNAPPEALGVASGLLAVVRTVGQTGGIALAGAIWASQIFAL
HHHHCCCHHEECCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEE
NGGPVTPITAAPPPILAGGYDIAMRVAALLAAIAIVPSLIGGYALQRQMAPARPPVAD
CCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1592819 [H]