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The map label for this gene is mdeA [H]

Identifier: 222524854

GI number: 222524854

Start: 2010996

End: 2012192

Strand: Reverse

Name: mdeA [H]

Synonym: Chy400_1581

Alternate gene names: 222524854

Gene position: 2012192-2010996 (Counterclockwise)

Preceding gene: 222524855

Following gene: 222524853

Centisome position: 38.19

GC content: 57.89

Gene sequence:

>1197_bases
ATGGCTATCGATCAGTTTACCGGACCCAGTACGGCCGCAGTTCACGCGGGTGAACCGCGTCAGCGTGCATTTGATGCCAT
CACCCCACCGGTTGTTCACAGTGCCACCTACACCTTCCGTGATAGCGCTGAGTTGATCGCTTTCCAGTCAGGCGACCTGG
AGCGTGAAGAGTACGGTCGCTACGGCAATCCGACTGTACGAGCCGTTGAAGCGCGATTAGCCGCTCTCGAATCACCAACG
GCACCTGCCGCAGCACTGCTTTGCGCTTCCGGGATGAATGCATTAACGACGGTGATGCTGGCACTTCTCCCATCAGGAAG
CCACGTTATTCTGACCGATGACGGTTATCGGCGCACCCGCCAGTTCGTGCGTACCATGCTGGCCCGGCTGGGTGTGACGC
ATAGCGTTGTAGCTGCCGCCGATCCTGCCGCGATTGCGGCTGCTATCGAGCCGGGACGCACTCGCCTGATTGTTACGGAA
GCGCCTACCAATCCTTACCTGCGGGTGATCGATCTGGCTGCCGTGGCAAGCATTGCCCGCGAGCATCGGATTAAGACGCT
CATCGATGCCACCTTCGCTACCCCCTACAATATGCGGCCACTGGAATACGGGATCGACCTGGTGGTGCATAGCTGTAGCA
AATATCTGGCAGGTCACAACGATCTGCTGGCCGGTGTGATCATTGGTCGGCCACCCATCATCAGTGCGCTGCGCGAAACT
CAGGGTATCCTCGGTGGTATCTGTGATCCGCATACCGCGTATCTGCTCGGTCGTGGGTTGAAGACCTTCGCCCTGCGCAT
GGAGCGCCACAATCACAATGGTCAGGCAGTAGCCGAATTTCTGGCCCGTCATCCACGGGTAAGCCGGGTGCATTATCCGG
GATTACCCGAACACCCCGACCACGCCGTCGCCCGTGCCCAAATGCGCGGCTTTGGCGGCGTTGTCTCATTTGAAGTAGTC
GGCGATTTGCAGAGTGCCATGGCTGTGGTCGACCGGTTGCGTCTGCCGTACATTGCACCGAGCTTTGGTGGTGTTGAAAG
CCTGGTTGAACAACCGGCACTTATGAGTTACTATGAATTGAGTAGTGAAGAACGGCTGGCGGTGGGTATTCGCGACAACC
TCATCCGCCTTTCGTGCGGGATTGAGGATGTGAGTGACTTGATCGCCGATCTGCAACAGGCTCTTGCCGACGAATAA

Upstream 100 bases:

>100_bases
GGCAGCATAAAGCTGGCAGATGAGAGGAGAGCGGCGTCTTCCCGTTAATCGGTAGAAGAACGTCATCTGACCTCTCGTCG
CCTTGATGAGAGGTTTTCTT

Downstream 100 bases:

>100_bases
CCTATGCATTCTACCCTTCAGCGCCGGCTCGCTGCCGGCCAGATTGTCGTTACCGGCGAGATTGCACCGCCAAAGGGGGC
GGCACGTAGCCATCTCGAAG

Product: Cystathionine gamma-synthase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MAIDQFTGPSTAAVHAGEPRQRAFDAITPPVVHSATYTFRDSAELIAFQSGDLEREEYGRYGNPTVRAVEARLAALESPT
APAAALLCASGMNALTTVMLALLPSGSHVILTDDGYRRTRQFVRTMLARLGVTHSVVAAADPAAIAAAIEPGRTRLIVTE
APTNPYLRVIDLAAVASIAREHRIKTLIDATFATPYNMRPLEYGIDLVVHSCSKYLAGHNDLLAGVIIGRPPIISALRET
QGILGGICDPHTAYLLGRGLKTFALRMERHNHNGQAVAEFLARHPRVSRVHYPGLPEHPDHAVARAQMRGFGGVVSFEVV
GDLQSAMAVVDRLRLPYIAPSFGGVESLVEQPALMSYYELSSEERLAVGIRDNLIRLSCGIEDVSDLIADLQQALADE

Sequences:

>Translated_398_residues
MAIDQFTGPSTAAVHAGEPRQRAFDAITPPVVHSATYTFRDSAELIAFQSGDLEREEYGRYGNPTVRAVEARLAALESPT
APAAALLCASGMNALTTVMLALLPSGSHVILTDDGYRRTRQFVRTMLARLGVTHSVVAAADPAAIAAAIEPGRTRLIVTE
APTNPYLRVIDLAAVASIAREHRIKTLIDATFATPYNMRPLEYGIDLVVHSCSKYLAGHNDLLAGVIIGRPPIISALRET
QGILGGICDPHTAYLLGRGLKTFALRMERHNHNGQAVAEFLARHPRVSRVHYPGLPEHPDHAVARAQMRGFGGVVSFEVV
GDLQSAMAVVDRLRLPYIAPSFGGVESLVEQPALMSYYELSSEERLAVGIRDNLIRLSCGIEDVSDLIADLQQALADE
>Mature_397_residues
AIDQFTGPSTAAVHAGEPRQRAFDAITPPVVHSATYTFRDSAELIAFQSGDLEREEYGRYGNPTVRAVEARLAALESPTA
PAAALLCASGMNALTTVMLALLPSGSHVILTDDGYRRTRQFVRTMLARLGVTHSVVAAADPAAIAAAIEPGRTRLIVTEA
PTNPYLRVIDLAAVASIAREHRIKTLIDATFATPYNMRPLEYGIDLVVHSCSKYLAGHNDLLAGVIIGRPPIISALRETQ
GILGGICDPHTAYLLGRGLKTFALRMERHNHNGQAVAEFLARHPRVSRVHYPGLPEHPDHAVARAQMRGFGGVVSFEVVG
DLQSAMAVVDRLRLPYIAPSFGGVESLVEQPALMSYYELSSEERLAVGIRDNLIRLSCGIEDVSDLIADLQQALADE

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=394, Percent_Identity=39.3401015228426, Blast_Score=245, Evalue=5e-65,
Organism=Homo sapiens, GI299473759, Length=394, Percent_Identity=36.5482233502538, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI25453487, Length=393, Percent_Identity=34.8600508905852, Blast_Score=192, Evalue=3e-49,
Organism=Escherichia coli, GI1790375, Length=390, Percent_Identity=36.6666666666667, Blast_Score=238, Evalue=7e-64,
Organism=Escherichia coli, GI1789383, Length=396, Percent_Identity=31.0606060606061, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI71980708, Length=375, Percent_Identity=38.4, Blast_Score=255, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI17538055, Length=390, Percent_Identity=38.2051282051282, Blast_Score=244, Evalue=7e-65,
Organism=Caenorhabditis elegans, GI17560056, Length=390, Percent_Identity=38.7179487179487, Blast_Score=241, Evalue=5e-64,
Organism=Saccharomyces cerevisiae, GI6319307, Length=400, Percent_Identity=35.75, Blast_Score=213, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=29.9539170506912, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6321254, Length=410, Percent_Identity=28.2926829268293, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6321170, Length=330, Percent_Identity=28.1818181818182, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6321904, Length=398, Percent_Identity=25.6281407035176, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19922554, Length=395, Percent_Identity=38.2278481012658, Blast_Score=268, Evalue=6e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 42955; Mature: 42823

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIDQFTGPSTAAVHAGEPRQRAFDAITPPVVHSATYTFRDSAELIAFQSGDLEREEYGR
CCCCCCCCCCCEEEECCCHHHHHHHHCCCCHHHCCEEEECCCCEEEEEECCCCCHHHCCC
YGNPTVRAVEARLAALESPTAPAAALLCASGMNALTTVMLALLPSGSHVILTDDGYRRTR
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCHHHHH
QFVRTMLARLGVTHSVVAAADPAAIAAAIEPGRTRLIVTEAPTNPYLRVIDLAAVASIAR
HHHHHHHHHHCCHHHHHHHCCCHHHEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHH
EHRIKTLIDATFATPYNMRPLEYGIDLVVHSCSKYLAGHNDLLAGVIIGRPPIISALRET
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCHHHHHHHHH
QGILGGICDPHTAYLLGRGLKTFALRMERHNHNGQAVAEFLARHPRVSRVHYPGLPEHPD
HHHHCCCCCCHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEECCCCCCCCC
HAVARAQMRGFGGVVSFEVVGDLQSAMAVVDRLRLPYIAPSFGGVESLVEQPALMSYYEL
HHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCHHHHHHHC
SSEERLAVGIRDNLIRLSCGIEDVSDLIADLQQALADE
CCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIDQFTGPSTAAVHAGEPRQRAFDAITPPVVHSATYTFRDSAELIAFQSGDLEREEYGR
CCCCCCCCCCEEEECCCHHHHHHHHCCCCHHHCCEEEECCCCEEEEEECCCCCHHHCCC
YGNPTVRAVEARLAALESPTAPAAALLCASGMNALTTVMLALLPSGSHVILTDDGYRRTR
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCHHHHH
QFVRTMLARLGVTHSVVAAADPAAIAAAIEPGRTRLIVTEAPTNPYLRVIDLAAVASIAR
HHHHHHHHHHCCHHHHHHHCCCHHHEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHH
EHRIKTLIDATFATPYNMRPLEYGIDLVVHSCSKYLAGHNDLLAGVIIGRPPIISALRET
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCHHHHHHHHH
QGILGGICDPHTAYLLGRGLKTFALRMERHNHNGQAVAEFLARHPRVSRVHYPGLPEHPD
HHHHCCCCCCHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEECCCCCCCCC
HAVARAQMRGFGGVVSFEVVGDLQSAMAVVDRLRLPYIAPSFGGVESLVEQPALMSYYEL
HHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCHHHHHHHC
SSEERLAVGIRDNLIRLSCGIEDVSDLIADLQQALADE
CCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]