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The map label for this gene is cobS [H]

Identifier: 222524587

GI number: 222524587

Start: 1678203

End: 1678964

Strand: Reverse

Name: cobS [H]

Synonym: Chy400_1311

Alternate gene names: 222524587

Gene position: 1678964-1678203 (Counterclockwise)

Preceding gene: 222524588

Following gene: 222524586

Centisome position: 31.87

GC content: 63.39

Gene sequence:

>762_bases
ATGAGCGACACATCGACACGGCCCACGCCTGGTTTGATCGAAGCGATCCGCTTTCTGACCATCATTCCGCTCCCCGGTCT
GTCACCGATGAGCGAACAGAGTGTGGTGCGGGCAATCCCGTGGTTTCCGGCAGTGGGGCTGGTGATTGGTGGAGTCTTGG
TGGCGGTTGACGCGCTGGCGCGTCCGTTGTGGGGCGATCTGACCGCTGCCATGCTGGTAGTCGTTGGGTGGGGGATTATC
ACCGGTGGCCTGCATCTCGATGGGCTGAGCGATACTTTCGATGCGGTGATGAGCTGGCGGTCGCGTGAGCGCAAGCTGGA
GATTATGAAAGATAGTCGGATCGGGGCGATGGGTGCCCTGGCGCTGATGGCTGTGCTGCTGCTGAAAGTGGCATTTGTGG
CCGGCGCGGCACCGGCCTGGCCGGCTCTTCTCCTGGCACCGATGCTGGGCCGTTGGGCAGATTGCTACGGCATCTATCGC
TTTCCGCCGGCGCGGGAAGGGGGGTTGGGGCGTACATTCAATGCGCAGGTACGCCAGTCCGATCTCTGGCTGGCATCGCT
GTTGATGGTCGCCAGTGCCTGGATTATCGCCGGCCTGGCCGGCCTGATCACGGTTGGCCTGGTGTTCGGCGTGGCTCACG
TGCTAGCGCGCTGGTGGGTGCGCGATCTGGGCGGATTGACCGGCGATACCTACGGTGCCCTCTGTGAGATTGGTGAGGTC
GTCGCGTTAGCCGCGCTTACCGTGCGGTGGGGGGGAATGTGA

Upstream 100 bases:

>100_bases
TTGATTTGGGGAGTATAACGGCCCTCGACACCTACGGTACGACGATCATTGTCCGCACCGTTAACGCGGTTCCGCGGTTG
GTGGGTGCAGGAGAGAGCGT

Downstream 100 bases:

>100_bases
TGCACACACGCACATGGCTCTGCTGGCTGATTGCCACTACCACCATCGCCATCCTGGCCCCGCACCCACTCTATCACATC
CTGCTCATGCTGGTCGTGAC

Product: cobalamin 5'-phosphate synthase

Products: Cobalamin 5'-Phosphate; GMP; Cobalamin [C]

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MSDTSTRPTPGLIEAIRFLTIIPLPGLSPMSEQSVVRAIPWFPAVGLVIGGVLVAVDALARPLWGDLTAAMLVVVGWGII
TGGLHLDGLSDTFDAVMSWRSRERKLEIMKDSRIGAMGALALMAVLLLKVAFVAGAAPAWPALLLAPMLGRWADCYGIYR
FPPAREGGLGRTFNAQVRQSDLWLASLLMVASAWIIAGLAGLITVGLVFGVAHVLARWWVRDLGGLTGDTYGALCEIGEV
VALAALTVRWGGM

Sequences:

>Translated_253_residues
MSDTSTRPTPGLIEAIRFLTIIPLPGLSPMSEQSVVRAIPWFPAVGLVIGGVLVAVDALARPLWGDLTAAMLVVVGWGII
TGGLHLDGLSDTFDAVMSWRSRERKLEIMKDSRIGAMGALALMAVLLLKVAFVAGAAPAWPALLLAPMLGRWADCYGIYR
FPPAREGGLGRTFNAQVRQSDLWLASLLMVASAWIIAGLAGLITVGLVFGVAHVLARWWVRDLGGLTGDTYGALCEIGEV
VALAALTVRWGGM
>Mature_252_residues
SDTSTRPTPGLIEAIRFLTIIPLPGLSPMSEQSVVRAIPWFPAVGLVIGGVLVAVDALARPLWGDLTAAMLVVVGWGIIT
GGLHLDGLSDTFDAVMSWRSRERKLEIMKDSRIGAMGALALMAVLLLKVAFVAGAAPAWPALLLAPMLGRWADCYGIYRF
PPAREGGLGRTFNAQVRQSDLWLASLLMVASAWIIAGLAGLITVGLVFGVAHVLARWWVRDLGGLTGDTYGALCEIGEVV
ALAALTVRWGGM

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=239, Percent_Identity=35.9832635983264, Blast_Score=114, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 26843; Mature: 26712

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHCCCC
>Mature Secondary Structure 
SDTSTRPTPGLIEAIRFLTIIPLPGLSPMSEQSVVRAIPWFPAVGLVIGGVLVAVDALA
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RPLWGDLTAAMLVVVGWGIITGGLHLDGLSDTFDAVMSWRSRERKLEIMKDSRIGAMGAL
HHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ALMAVLLLKVAFVAGAAPAWPALLLAPMLGRWADCYGIYRFPPAREGGLGRTFNAQVRQS
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
DLWLASLLMVASAWIIAGLAGLITVGLVFGVAHVLARWWVRDLGGLTGDTYGALCEIGEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
VALAALTVRWGGM
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-Cobinamide; Alpha-Ribazole 5'-Phosphate; Alpha-Ribazole [C]

Specific reaction: GDP-Cobinamide + Alpha-Ribazole 5'-Phosphate (or Alpha-Ribazole) = Cobalamin 5'-Phosphate (or GDP-Cobalamin) + GMP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA