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The map label for this gene is purB [H]

Identifier: 222524553

GI number: 222524553

Start: 1634548

End: 1635948

Strand: Reverse

Name: purB [H]

Synonym: Chy400_1277

Alternate gene names: 222524553

Gene position: 1635948-1634548 (Counterclockwise)

Preceding gene: 222524561

Following gene: 222524552

Centisome position: 31.05

GC content: 57.39

Gene sequence:

>1401_bases
ATGACAACAGACGATATGACGCGGCTGGCGGCACTGGGACCACTCGATGGCCGTTACCGTGCTGATGTGGCAAGTCTGGC
CAGCTTTTTCAGTGAAGCTGCTCTCTTTCGCTATCGTGTGCGGGTAGAGGTGGAATATCTCATCTTTCTCTCCCGTGCCC
GTGGCATCCATTTTGTCCCGCCGCTGACCCCGCCGCAACAGGCTGCGCTCCGTGCCCTCTACCGTCAGTTTGGTGATGAT
GATGCGCTGGCGATTGCGGCATGGGATCGGCGTGTCAATCACGATGTGAAAGCGGTTGAGTACTGGTTACGCGAACGGTT
AACAACACTCGGTTTAACCGGTCATCTGGAAGCGGTGCATTTCGCGCTGACCTCTGAAGATGTGAACAATCTGGCGTATG
CGCTGATGGTCAAAGAGGCACGTGAAATGGTCTTGCTGCCGGCACTTGAGTCCTTGCTCGAACGCATTCGGCAGTTGGCC
GACGAGGAAGCAGCGACGCCGATGCTGGCCCGCACGCACGGACAACCCGCGACGCCAACAACGTTCGGCAAAGAGATGAA
CGTCTTCTTTATGCGGTTACGACGGGCGATCAACGACATGATGGCGATCAAGATCACCGGTAAGCTCAACGGCGCAAGTG
GTGTTTTTGCCGCCCACCACGTTGCCCTGCCCCAGATCGACTGGCTAAAATTCTCGCGTGCCTTTGTGCGTTCGCTCGAT
CTGGAACCGATTTTGCTCACGACCCAGATTGAACCGCACGACACACTGGCAGCGCTCTGCGATGCGCTCAAACGGATTGG
CGCAATTCTGACCGACCTTAGCCAGGATTGCTGGCGCTACATTAGCGATGGTTATCTGGTACAGGCGGCCCGGGCCGGTG
AGGTTGGCTCTTCAACTATGCCGCATAAGGTGAATCCTATCGATTTTGAAAATGCCGAAGGCAATCTGGCCCTGGCCGGT
GCTTTGCTGGAATTGTTCAGTCGTAAGCTACCGGTTTCGCGCCTCCAACGCGATCTCTCCGACAGCACGGTCTTGCGTAA
TCTGGGTCTGGCCTTCGGCTATTGTCTCCTCGCTTACCAGCGCCTGCTGCGCGGCCTGCAAAAAGTGGCTGTTGATCGCG
CCCGCTTGCGCCGCGATCTCGATGCGCATCCCGAAGTCCTGGCCGAGGCCATTCAGACAATCCTCCGCCGTGAGGGTTTT
GCTGAACCCTACGAGTTGTTGAAGTCTTTTTCGCGTGGTCGTGCGCTGACTGCCGAAGATATTGCCCGCTTCATCGACAG
TTTACTGGTCAGTGATGCGGTGCGGGCCGAGTTGCAGGCGCTCGCACCTGCGGCGTATATCGGTCTGGCGGTCAAGCTTG
CCCAACTACGCGATGAGGCGACAGTTGGTAACTGGACGTAG

Upstream 100 bases:

>100_bases
TATTCAACGCCGACGGCGTCAGTAATCGCTCGTTACTCACCACTTGCGATTTCTATGGTACCCTTCGTAACGGCAACAAC
TCGTGGAACAGGAGGCAAGT

Downstream 100 bases:

>100_bases
CGATAGTGCAATATAATTCCACTGTCACGCTGATCACTGTCGTGACAGTGGAAGAGTTTGTTGCCGGTACATGCACAATT
TTATATTTGCAATCTATTGA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MTTDDMTRLAALGPLDGRYRADVASLASFFSEAALFRYRVRVEVEYLIFLSRARGIHFVPPLTPPQQAALRALYRQFGDD
DALAIAAWDRRVNHDVKAVEYWLRERLTTLGLTGHLEAVHFALTSEDVNNLAYALMVKEAREMVLLPALESLLERIRQLA
DEEAATPMLARTHGQPATPTTFGKEMNVFFMRLRRAINDMMAIKITGKLNGASGVFAAHHVALPQIDWLKFSRAFVRSLD
LEPILLTTQIEPHDTLAALCDALKRIGAILTDLSQDCWRYISDGYLVQAARAGEVGSSTMPHKVNPIDFENAEGNLALAG
ALLELFSRKLPVSRLQRDLSDSTVLRNLGLAFGYCLLAYQRLLRGLQKVAVDRARLRRDLDAHPEVLAEAIQTILRREGF
AEPYELLKSFSRGRALTAEDIARFIDSLLVSDAVRAELQALAPAAYIGLAVKLAQLRDEATVGNWT

Sequences:

>Translated_466_residues
MTTDDMTRLAALGPLDGRYRADVASLASFFSEAALFRYRVRVEVEYLIFLSRARGIHFVPPLTPPQQAALRALYRQFGDD
DALAIAAWDRRVNHDVKAVEYWLRERLTTLGLTGHLEAVHFALTSEDVNNLAYALMVKEAREMVLLPALESLLERIRQLA
DEEAATPMLARTHGQPATPTTFGKEMNVFFMRLRRAINDMMAIKITGKLNGASGVFAAHHVALPQIDWLKFSRAFVRSLD
LEPILLTTQIEPHDTLAALCDALKRIGAILTDLSQDCWRYISDGYLVQAARAGEVGSSTMPHKVNPIDFENAEGNLALAG
ALLELFSRKLPVSRLQRDLSDSTVLRNLGLAFGYCLLAYQRLLRGLQKVAVDRARLRRDLDAHPEVLAEAIQTILRREGF
AEPYELLKSFSRGRALTAEDIARFIDSLLVSDAVRAELQALAPAAYIGLAVKLAQLRDEATVGNWT
>Mature_465_residues
TTDDMTRLAALGPLDGRYRADVASLASFFSEAALFRYRVRVEVEYLIFLSRARGIHFVPPLTPPQQAALRALYRQFGDDD
ALAIAAWDRRVNHDVKAVEYWLRERLTTLGLTGHLEAVHFALTSEDVNNLAYALMVKEAREMVLLPALESLLERIRQLAD
EEAATPMLARTHGQPATPTTFGKEMNVFFMRLRRAINDMMAIKITGKLNGASGVFAAHHVALPQIDWLKFSRAFVRSLDL
EPILLTTQIEPHDTLAALCDALKRIGAILTDLSQDCWRYISDGYLVQAARAGEVGSSTMPHKVNPIDFENAEGNLALAGA
LLELFSRKLPVSRLQRDLSDSTVLRNLGLAFGYCLLAYQRLLRGLQKVAVDRARLRRDLDAHPEVLAEAIQTILRREGFA
EPYELLKSFSRGRALTAEDIARFIDSLLVSDAVRAELQALAPAAYIGLAVKLAQLRDEATVGNWT

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=450, Percent_Identity=47.7777777777778, Blast_Score=426, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51921; Mature: 51789

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDDMTRLAALGPLDGRYRADVASLASFFSEAALFRYRVRVEVEYLIFLSRARGIHFVP
CCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHCCCEEECC
PLTPPQQAALRALYRQFGDDDALAIAAWDRRVNHDVKAVEYWLRERLTTLGLTGHLEAVH
CCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHH
FALTSEDVNNLAYALMVKEAREMVLLPALESLLERIRQLADEEAATPMLARTHGQPATPT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCC
TFGKEMNVFFMRLRRAINDMMAIKITGKLNGASGVFAAHHVALPQIDWLKFSRAFVRSLD
CCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCC
LEPILLTTQIEPHDTLAALCDALKRIGAILTDLSQDCWRYISDGYLVQAARAGEVGSSTM
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
PHKVNPIDFENAEGNLALAGALLELFSRKLPVSRLQRDLSDSTVLRNLGLAFGYCLLAYQ
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLRGLQKVAVDRARLRRDLDAHPEVLAEAIQTILRREGFAEPYELLKSFSRGRALTAED
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHH
IARFIDSLLVSDAVRAELQALAPAAYIGLAVKLAQLRDEATVGNWT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TTDDMTRLAALGPLDGRYRADVASLASFFSEAALFRYRVRVEVEYLIFLSRARGIHFVP
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHCCCEEECC
PLTPPQQAALRALYRQFGDDDALAIAAWDRRVNHDVKAVEYWLRERLTTLGLTGHLEAVH
CCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHH
FALTSEDVNNLAYALMVKEAREMVLLPALESLLERIRQLADEEAATPMLARTHGQPATPT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCC
TFGKEMNVFFMRLRRAINDMMAIKITGKLNGASGVFAAHHVALPQIDWLKFSRAFVRSLD
CCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCC
LEPILLTTQIEPHDTLAALCDALKRIGAILTDLSQDCWRYISDGYLVQAARAGEVGSSTM
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
PHKVNPIDFENAEGNLALAGALLELFSRKLPVSRLQRDLSDSTVLRNLGLAFGYCLLAYQ
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLRGLQKVAVDRARLRRDLDAHPEVLAEAIQTILRREGFAEPYELLKSFSRGRALTAED
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHH
IARFIDSLLVSDAVRAELQALAPAAYIGLAVKLAQLRDEATVGNWT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]